细析考点 交融层级 活化方法——2010年“默写常见的名句名篇”的有效复习策略

“默写常见的名句名篇”的有效复习策略是：在细析考点的基础上，交融层级、活化方法。“交融层级”指在默写名句名篇复习中，不要拘泥于该考点考试层级为识记层级（A）的规定，复习层级不等同于考试层级。具体地，要以理解层级（B）、应用层级（E）和识记层级（A）交融的方式，复习考试层级为识记层级（A）的默写名句名篇的考点。因为理解的甚至能应用的知识才能更有效地掌握。“活化方法”指在“默写名句名篇”的复习中。避免一味死记硬背的方法，活化优化记忆方法，从而达到有效、高效的复习效果。

基于多任务学习的同行评审细粒度情感分析模型

学术论文同行评审能够直接反映审稿人对论文的主观评价,对审稿文本进行情感分析有利于挖掘审稿人对论文多维度的评价信息。现有的情感分析模型仅能挖掘专家单一的评审维度和相应的情感倾向,本文提出了一种基于多任务学习的同行评审细粒度情感分析模型。该模型在多任务学习框架下,通过在BERT-LCF模型的基础上增加BiLSTM-CRF模块,使其具备了同时完成属性词抽取和细粒度情感分析任务的能力。与传统的基于Pipeline模式的单任务细粒度情感分析模型相比,本模型在保证精度的情况下可以同时完成评审属性提取和情感分析任务。在这两项任务中,所提出模型的F1分数分别达到了89.01%和90.71%。对比实验证明,在多任务场景下,引入BiLSTM-CRF模块对评审文本属性词提取任务有一定的提升作用。

血细胞分析仪计数血小板的准确性评价

目的了解自动化血细胞分析仪计数血小板的准确性以及影响其准确计数的因素。方法对血细胞分析仪计数的血小板在100—300×109／L50例健康人，70例血小板减少（PLT〈100×10^9／L）的患者，60例血小板增多（PLT〉300×10^9／L）的患者进行末梢血手工显微镜计数法计数血小板作为参考方法，比较两种方法计数血小板数目的差异，进行统计分析。结果50例健康人两种方法计数血小板差异无统计学意义t=0．91，P〉0.05）．血小板〈100×10^9／L的70患者中58例两种方法计数血小板差异无统计学意义（t=0．78，P〉0．05）,12例两种计数方法差异有统计学意义（t=3．67，P〈0．05）。计数PLT）300×10^9／L60例患者中52例两种方法计数血小板差异无统计学意义（t=0．56，P〉0．05），8例两种计数法计数血小板差异有统计学意义t=3．72，P〈0．05）。结论血细胞分析仪计数血小板的准确性易受EDTA抗剂剂引起的血小板聚集和小红细胞的影响。对血小板数目异常的，与临床联系，如与临床症状不符，要进行末梢血手工计数法计数血小板。

Determination of Five Organic Acids in Radix Isatidis by Column Partition Chromatography and Capillary Zone Electrophoresis柱分配色谱-毛细管区带电泳联用分析板蓝根中五种微量有机酸(英文)

Aim To determine five organic acids in Radix Isatidis . Method The extraction method and the column partition chromatographic conditions were studied. Then a capillary zone electrophoretic method was set up for the determination. Results The linear ranges of quinazolinone acid, n anthranilic acid, benzoic acid, salicylic acid, and syringic acid were 5 52-92 0 μg·mL -1 , 5 12-102 μg·mL -1 , 2 28-84 4 μg·mL-1 , 4 78-159 μg·mL -1 , and 1 74-87 0 μg·mL -1 respectively. Conclusion The established method is accurate and simple.

Bacterial Protein Profiling——Comparison of Three Mass Spectrometry Methodologies

Profiling the protein composition of bacteria is essential for understanding their biology,physiology and interaction with environment.Mass spectrometry has become a pivotal tool for protein analysis,facilitating the examination of expression levels,molecular masses and structural modifications.In this study,we compared the performance of three widely-used mass spectrometry methods,i.e.,matrix-assisted laser desorption/ionization(MALDI)protein fingerprinting,top-down proteomics and bottom-up proteomics,in the profiling of bacterial protein composition.It was revealed that bottom-up proteomics provided the highest protein coverage and exhibited the greatest protein profile overlap between bacterial species.In contrast,MALDI protein fingerprinting demonstrated superior detection reproducibility and effectiveness in distinguishing between bacterial species.Although top-down proteomics identified fewer proteins than bottom-up approach,it complemented MALDI fingerprinting in the discovery of bacterial protein markers,both favoring abundant,stable,and hydrophilic bacterial ribosomal proteins.This study represents the most systematic and comprehensive comparison of mass spectrometry-based protein profiling methodologies to date.It provides valuable guidelines for the selection of appropriate profiling strategies for specific analytical purposes.This will facilitate studies across various fields,including infection diagnosis,antimicrobial resistance detection and pharmaceutical target discovery.

怎样开发《雨的四季》的审辨思维资源

我们把《雨的四季》这篇课文所存在的问题当作审辨思维资源来开发,真备课,真讲课,讲真课,避免人云亦云、颠倒是非、混淆美丑,引导学生分析问题、解决问题,构建生动课堂,有助于学生的批判性、创造性等思维品质的培养。

血细胞分析仪计数血小板的准确性评价

目的了解自动化血细胞分析仪计数血小板的准确性以及影响其准确计数的因素。方法对血细胞分析仪计数的血小板(PLT)在100～300×109/L50例健康人,70例PLT减少(PLT<100×109/L)的患者,60例PLT增多(PLT>300×109/L)的患者进行末梢血手工显微镜计数法计数PLT作为参考方法,比较2种方法计数PLT的准确性。结果 50例健康人2种方法计数PLT差异无统计学意义(t=0.91,P>0.05),PLT<100×109/L的70例患者中58例2种方法计数PLT差异无统计学意义(t=0.78,P>0.05),12例假性减少,2种计数方法差异有统计学意义(t=3.67,P<0.05)。PLT>300×109/L60例患者中52例2种方法计数PLT差异无统计学意义(t=0.56,P>0.05),8例假性增多,2种计数法差异有统计学意义(t=3.72,P<0.05)。结论血细胞分析仪计数PLT的准确性易受EDTA抗剂剂引起的PLT聚集和体积较小的红细胞的影响。PLT数目异常时,应与临床联系,如与临床症状不符,应进行末梢血手工计数法计数PLT。

Cell Cycle Kinetic Analysis in the Cortical Regions of the Lentil Primary Root During Germination

Cell cycle kinetic activity in the cortical cells of the lentil (Lens culinaris Me-die. cv. Verte du Puy) primary root during germination was examined both temporally and spatially. Immunohistochemical and cytological evidence indicated that DNA replication and cell division started in the cortical cells of tire lentil primary root after around 13 and 17 h of imbibition, respectively. The first cells in DNA synthesis and the First mitotic figures all appeared in the cortical cells about I mm front the root-cap junction, but these divided cells had synthesized their DNA during the maturity of seed instead of during germination. The kinetic pattern of activity of the first cell cycle showed that these cells were not activated synchronously, but re-entered the cell cycle in turn depending on their places in the root tip, However, the adjacent cells partially synchronously proceeded their cell cycle.

AFLP Analysis of Cytoplasmic Male-sterile Line and Its Maintainer Line of Pepper

[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers.[Method] Cytoplasmic male sterility(CMS)line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A,while the specific amplified fragments were recovered or sequenced,and analyzed by BLAST...

Determination of IL-2/IL-2R system in patients with liver cirrhosis.and carcinoma

AIMS To.determine the interleuking-2 and interleukin- 2 receptor (IL-2/IL-2R) system in patients with liver cirrhosis and carcinoma and compare their immune functions. The clinical significance is also discussed. METHODS Fifty patients with Liver cirrhosis (LC), 50 patients with hepatocellular carcinoma (HCC) and 30 normal controls were studied. The expression of mlL-2R was examined by immunofluorescence. IL-2's activity and serum level of soluble interleukin-2 recep- tor (sIL-2R) were measured by enzyme linked im- munosorbent assay. RESULTS IL-2's activity and the percentage of mIL- 2R expression in carcinoma were significantly lower than those in cirrhosis (P<0.01) and controls (P< 0.01),while the IL-2's activity and the expression of mlL-2R in cirrhosis were also lower than normal controls (P<0.05). The serum level of sIL-2R in carcinoma was significantly higher than that in cirrhosis (P<0.05) and controls (P<0.01),and the level in cirrhosis was higher than in controls (P<0.05). CONCLUSIONS Patients with liver cirhosis and car- cinoma share the decreased immune function of similar nature,but the latter has a more profound degree. Such resemblance in immune disturbances may be the important factor affecting the carcinogenesis of cirrhotic liver.

Meso-mechanical analyses of shape memory alloy wires reinforced smart structures with damages

The thermo-mechanical behaviors of shape memory alloy (SMA) wires reinforced smart structures with damages are analyzed through variational principle and meso-mechanical method.A governing equation on the structure is derived.Mathematical expressions on meso-displacement field,stress-strain field of typical element with damages are presented.A failure criterion for interface failure between SMA wires and matrix is established under two kinds of actuation which are dead-load and temperature,where the temperature is included in effective free restoring strain.In addition,there are some other composing factors in the failure criterion such as interface properties,thermodynamical properties of SMA,initial debonding length L-l,etc.The results are significant to understand structural strength self-adaptive control and failure mechanism of SMA wires reinforced smart structures with damages,and provide a theoretical foundation for further study on the integrity of SMA smart structures.

Effect of minor Sc and Zr addition on microstructure and properties of ultra-high strength aluminum alloy

The Al-9Zn-2.8Mg-2.5Cu-xZr-ySc alloys （x=0, 0.15%, 0.15%; y=0, 0.05%, 0.15%）, produced by low-frequent electromagnetic casting technology, were subjected to homogenization treatment, hot extrusion, solution and aging treatment. The effects of minor Sc and Zr addition on microstructure, recrystallization and properties of alloys were studied by optical microscopy （OM）, scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. The results show that Sc and Zr addition can refine grains of the as-cast alloy by precipitation of primary Al3（Sc,Zr） particles formed during solidification as heterogeneous nuclei. Secondary Al3（Sc,Zr） precipitates formed during homogenization treatment strongly pin the movement of dislocation and subgrain boundaries, which can effectively inhibit the alloys recrystallization. Compared with the alloy without Sc and Zr addition, the Al-Zn-Mg-Cu-Zr alloy with 0.05%Sc and 0.15%Zr shows the increase in tensile strength and yield strength by 172 MPa and 218 MPa, respectively. Strengthening comes from the contributions of precipitation, substructure and grain refining.

以生为本,建构初中数学有效课堂

本文论述了构建初中数学有效课堂的策略与措施，从善提会问激发学生思考，精讲细析突破学生疑问，适时归纳提升学生能力三方面进行详细分析，不断提高学生的数学素养，提高教学效率。

A Novel Three-parameter Flow Cytometric Analysis for Cell Cycle

To set up a three-parameter method for cell cycle analysis by two-laser flowcy-tometer, which can detect two types of cyclin plus DNA content in one measurement, and thatanalyze unscheduled expression of cyclins. Methods: Three-color fluorescence was used for analysisof two types of cyclins and DNA content simultaneously in individual cells by two-laser flowcytometry. MOLT-4 cells were used to study the expression of major cyclins in mammalian cells. ATriton-X100 permeabilization procedure was optimized for detection of two types of cyclins. Onecyclin was stained directly with a FITC-conjugated monoclonal antibody (mAb), and the other,indirectly with RPE-Cy5-conjugated secondary antibody, while DNA was stained with the fluorochromeDAPI. mAMSA and mimosine treated MOLT-4 cells were used to test this three-parameter method.Results: Permeabilization with 0.5% Triton-XlOO in PBS containing 1% BSA for 5 min on ice providedoptimal conditions for the simultaneous labelling of two cyclins plus DNA in single cells. It wasfound that the emission spectrum of the three dyes (DAPI, FITC and RPE-Cy5) could be measured withno compensation. Based on cyclinA/cyclinE/DNA flow cytometric analysis, asynchronously growingMOLT-4 cells could be divided into 6 compartments (G1o, G1e, G1l, S, G2, and M) simultaneously,allowing for analysis of cell cycle phase specific perturbations without the necessity of cellsynchronization. Unscheduled cyclin B1 expression was observed in G1 cells treated with mimosine andcyclin E in G2 cells treated with mAMSA. We found that unscheduled cyclin expression paralleledexpected cyclin expression. Conclusion: Thus, three-color FCM analysis of cells may not only beapplied to measure unscheduled vs. expected cyclin expression but may also be used to estimate thefraction of cycling cells in up to 6 cell populations.

Effect of electromagnetic field on microstructure and macrosegregation of flat ingot of 2524 aluminium alloy

Low frequency electromagnetic casting （LFEC） process with the application of an induction coil outside the conventional direct chill （DC） casting mould was used to prepare the flat ingot of 2524 alloy and the effect of electromagnetic field on the microstructure and macrosegregation of this alloy was systematically studied. The results show that the fiat ingot prepared by the LFEC process has a finer and more uniform as-cast microstructure and the grain morphology is transformed from dendrite and rosette-like to equiaxed structure. The LFEC process also shows a significant effect on macrosegregation, and with the application of electromagnetic field during casting process, the segregation in the centre of the ingot is obviously reduced. The mechanism of these effects was also discussed.

Characterization of a Cyclophilin cDNA from Soybean Cells

A cDNA clone encoded for cyclophilin (GmCyp1) was isolated by RT-PCR method from suspension-cultured soybean (Glycine max L.) cells. The deduced amino acid sequence was 91% identical to a kidney bean cyclophilin in the open reading frame of the gene. Results from Southern blotting analysis suggests that the GmCyp1 belong to a small gene family in soybean cells. The time course of GmCyp1 mRNA accumulation upon treatment of elicitor from yeast extract did not show significant change in the time period examined. The data suggest that the GmCyp1 was not regulated much by biotic factors. A possible role of the cyclophilin in the plant-pathogen interaction was discussed.

Fine-grained Mg−1Mn−0.5Al−0.5Ca−0.5Zn alloy with high strength and good ductility fabricated by conventional extrusion

Mg−1Mn−0.5Al−0.5Ca−0.5Zn(wt.%)alloy was fabricated by conventional extrusion at 673 K with an extrusion ratio of 25:1,followed by aging at 473 K.The microstructure was characterized by scanning electron microscopy,electron back-scattered diffraction,and transmission electron microscopy.The mechanical properties were determined by the tensile test.The peak-aged sample shows fine recrystallized grains with an average grain size of 1.7μm.Area fraction of Al−Ca particles in the alloy increases significantly after peak aging.Meanwhile,botháañandác+añdislocations were observed to remain in the alloy after hot extrusion.Thus,the peak-aged sample exhibits simultaneously high strength and good ductility with the ultimate tensile stress,tensile yield stress,and tension fracture elongation of 320 MPa,314 MPa,and 19.0%,respectively.

Ascitic fluid analysis for diagnosis and monitoring of spontaneous bacterial peritonitis

Polymorphonuclear (PMN) cell count in the ascitic fluid is essential for the diagnosis and management of spontaneous bacterial peritonitis (SBP). To date, PMN cell count is routinely performed by traditional manual counting. However, this method is time-consuming, costly, and not always timely available. Therefore, considerable efforts have been made in recent years to develop an alternative test for a more rapid diagnosis and monitoring of SBP. The use of urinary reagent strips was proposed to achieve an "instant" bedside diagnosis of SBP. A series of reports evaluated the urine strip test for SBP diagnosis and reported promising results. However, a recent large multicenter study revealed a surprising lack of diagnostic effi cacy of the urine screening test for SBP diagnosis. Another method, more recently proposed as an alternative to the manual PMN count, is the measurement of lactoferrin in ascitic fluid, but the data available on the diagnostic value of this test are limited to a single study. However, both urinary reagent strips and ascitic lactoferrin tests are qualitative methods and need, therefore, to be further confirmed by standard cytology of the ascitic fluid. To date, the only quantitative method proposed as a valid alternative to manual PMN counting is automated blood cell counters, commonly used in all laboratories for blood cell counting. Data available in the literature on the diagnostic performance of this method are limited but very promising, and this tool seems to have the potential to replace the manual counting method.

Cloning and expression analysis of a ubiquitin gene (Ub_(L40)) in the haemocytes of Crassostrea hongkongensis under bacterial challenge

Ubiquitin, a highly conserved stress-related protein, is assigned multiple functions, such as DNA processing, protein degradation, and ribosome synthesis. The Crassostrea hongkongensis ubiquitin gene (designated ChUbL40) was cloned by a combination of suppressive subtractive hybridization (SSH) and rapid amplification of cDNA ends (RACE). The full-length cDNA of ChUbL40 is 496 bp in length, consisting of a 5' untranslated region (UTR) of 34 bp, a 3'-UTR of 75 bp and an open reading frame of 387 bp encoding a ubiquitin fusion protein of 128 amino acids. Analysis of the amino acid sequence of ChUbL40 reveals that UbL40 is highly conservative during evolution. The expression patterns of ChUbL40 gene in various tissues were examined by real-time PCR. The expression level of ChUbL40 in haemocytes is down-regulated at 4 h and gradually returned to its original level from 6 h to 24 h after Vibrio alginolyticus challenge. Our results suggest that ChUbL40 is ubiquitously expressed and plays an important role in immune defense against bacterial challenge.

miR-200 family expression is downregulated upon neoplastic progression of Barrett's esophagus

AIM: To investigate miR-200 family expression in Barrett's epithelium, gastric and duodenal epithelia, and esophageal adenocarcinoma. METHODS: Real-time reverse transcriptase-polymerase chain reaction was used to measure miR-200, ZEB1 and ZEB2 expression. Ingenuity Pathway Analysis of miR-200 targets was used to predict biological outcomes. RESULTS: Barrett's epithelium expressed lower levels of miR-141 and miR-200c than did gastric and duodenal epithelia (P < 0.001). In silico analysis indicated roles for the miR-200 family in molecular pathways that distinguish Barrett's epithelium from gastric and duodenalepithelia, and which control apoptosis and proliferation. All miR-200 members were downregulated in adenocarcinoma (P < 0.02), and miR-200c expression was also downregulated in non-invasive epithelium adjacent to adenocarcinoma (P < 0.02). The expression of all miR-200 members was lower in Barrett's epithelium derived high-grade dysplastic cell lines than in a cell line derived from benign Barrett's epithelium. We observed signif icant inverse correlations between miR-200 family expression and ZEB1 and ZEB2 expression in Barrett's epithelium and esophageal adenocarcinoma (P < 0.05). CONCLUSION: miR-200 expression might contribute to the anti-apoptotic and proliferative phenotype of Barrett's epithelium and regulate key neoplastic processes in this epithelium.