The nature of the slurry from the stone-crushing and sand-making processes is analyzed to develop a novel separation process.The process comprises hydro-cyclone separation followed by screening of the fines,clarificat...The nature of the slurry from the stone-crushing and sand-making processes is analyzed to develop a novel separation process.The process comprises hydro-cyclone separation followed by screening of the fines,clarification,and filtration.Recovering fine sand and clean wastewater for recycle is demonstrated.The +0.045 mm fine sand fraction and à0.045 mm ultra-fine clay in the slurry are separated and recovered.Fine sand that was previously lost and wasted is now recoverable.The cleaned and reused water is as much as 94% of the total.展开更多
Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage ...Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage line U937 (as control) and U937 with MCP-1 was extracted, made reverse transcript to cDNA and tested with gene expression chip HO2 human. Results: Some chemotactic-related gene expressions were changed in all analyzed genes. Regulated upon activation, normal T cell expressed and secreted (RANTES) was up-regulated over 2-fold and 7 chemotactic-related genes (CCR2, CCR5, CCL16, GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2) were down-regulated over 2-fold in MCP-1 treated U937 cells at mRNA level. Conclusion: MCP-1 can influence some chemokines and receptors expression in macrophage in vitro, in which MCP-1 mainly down-regulates the chemotactic genes expression of those influencing neutrophilic granulocyte (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2). Another novel finding is that it can also down-regulate the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.展开更多
基金financially supported by Xinkaiyuan Crushed Stones Co. Ltd
文摘The nature of the slurry from the stone-crushing and sand-making processes is analyzed to develop a novel separation process.The process comprises hydro-cyclone separation followed by screening of the fines,clarification,and filtration.Recovering fine sand and clean wastewater for recycle is demonstrated.The +0.045 mm fine sand fraction and à0.045 mm ultra-fine clay in the slurry are separated and recovered.Fine sand that was previously lost and wasted is now recoverable.The cleaned and reused water is as much as 94% of the total.
文摘Objective: To study the chemotactic superfamily genes expression profiling of macrophage line U937 treated with monocyte chemoattractant protein-1 (MCP-1) using gene chip technique. Methods: Total RNA from macrophage line U937 (as control) and U937 with MCP-1 was extracted, made reverse transcript to cDNA and tested with gene expression chip HO2 human. Results: Some chemotactic-related gene expressions were changed in all analyzed genes. Regulated upon activation, normal T cell expressed and secreted (RANTES) was up-regulated over 2-fold and 7 chemotactic-related genes (CCR2, CCR5, CCL16, GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2) were down-regulated over 2-fold in MCP-1 treated U937 cells at mRNA level. Conclusion: MCP-1 can influence some chemokines and receptors expression in macrophage in vitro, in which MCP-1 mainly down-regulates the chemotactic genes expression of those influencing neutrophilic granulocyte (GROβ, GROγ, IL-8 and granulocyte chemotactic protein 2). Another novel finding is that it can also down-regulate the mRNA level of CCR5, which plays a critical role in many disorders and illnesses.
