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细粒焦渣对废水中酚的吸附研究 被引量:3
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作者 黄念东 夏畅斌 李德良 《湘潭矿业学院学报》 EI 2000年第2期63-66,共4页
利用焦化厂细粒焦渣对苯酚的吸附 ,研究了各种因素 (焦粒大小、pH值和溶液的滤速等 )对吸附的影响 结果表明 ,在 2 5℃ ,滤速为 4 .5mL/min ,pH为 2~ 2 .5,浓度为 30mg/L的苯酚溶液中 ,酚的去除率可达 98% 探讨了细粒焦渣对焦化厂含... 利用焦化厂细粒焦渣对苯酚的吸附 ,研究了各种因素 (焦粒大小、pH值和溶液的滤速等 )对吸附的影响 结果表明 ,在 2 5℃ ,滤速为 4 .5mL/min ,pH为 2~ 2 .5,浓度为 30mg/L的苯酚溶液中 ,酚的去除率可达 98% 探讨了细粒焦渣对焦化厂含酚废水处理的可行性 图 4 ,参 展开更多
关键词 细粒焦 吸附性能 废水处理 化厂
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细粒焦对废水中酚的吸附研究 被引量:1
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作者 陈津端 许中坚 《湘潭师范学院学报(自然科学版)》 2004年第2期20-22,共3页
用焦化厂细粒焦对废水中苯酚进行吸附 ,并研究了各种主要因素对苯酚吸附率的影响。实验结果表明 :在2 5℃时 ,细粒焦的用量为 2 .5 % ,细粒焦粒径为 0 .0 76 0 .0 97mm ,pH =2 .0 ,反应时间为 12 0min ,在浓度为 4 0mg/L的苯酚溶液中 ,... 用焦化厂细粒焦对废水中苯酚进行吸附 ,并研究了各种主要因素对苯酚吸附率的影响。实验结果表明 :在2 5℃时 ,细粒焦的用量为 2 .5 % ,细粒焦粒径为 0 .0 76 0 .0 97mm ,pH =2 .0 ,反应时间为 12 0min ,在浓度为 4 0mg/L的苯酚溶液中 ,苯酚的去除率可达 98%以上。同时探讨了细粒焦对焦化厂含酚废水处理的可行性。 展开更多
关键词 细粒焦 废水处理 苯酚 吸附性能
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Construction of recombinant plasmid pEGFP-C2-L539fs/47 and its expression in HEK293 cells 被引量:2
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作者 Lue Ying Zhang Aifeng +6 位作者 Han Wenqi Li Guoliang Zhang Junbo Gao Jie Pan Junqiang Zhang Yong Sun Chaofeng 《Journal of Medical Colleges of PLA(China)》 CAS 2012年第3期125-133,共9页
Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusion protein expressed in HEK293 cells(human embryo kidney cells).Methods:After double digestion of... Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusion protein expressed in HEK293 cells(human embryo kidney cells).Methods:After double digestion of pcDNA3-L539fs/47 and pEGFP-C2-HERG with sbf I and Eco91 I,the small product fragment,from pcDNA3-L539fs/47,was subcloned into the big fragment of pEGFP-C2-HERG under T4 ligase.pEGFP-C2-L539fs/47 was identified by agarose gel electrophoresis and sequencing.pcDNA3-L539fs/47 and pEGFP-C2-L539fs/47 were transiently transfected into HEK293 cells by Lipofect,respectively.The expression of fusion protein in HEK293 cells was detected through immunofluorescence,laser confocal imaging scanning in vivo,Western blot and PCR.Results:Mutation region cDNA fragment(about 1 kb) and target vector fragment(about 7.2 kb) were ligated after purification and gel recovery.Agarose gel electrophoresis and sequencing successfully demonstrated eukaryotic expression plasmid pEGFP-C2-L539fs/47,constructed approximately 8.2 kb,sequencing consistent with template gene.The transfection efficiency of recombinant plasmid by fluorescence microscopy was more than60%.Western blot analysis detected pcDNA3-L539fs/47 expression of the protein size 60 KD,the expression of pEGFP-C2 fusion protein size of approximately 90 KD.The L539fs/47 gene expression in HEK293 cells was significant by PCR analysis.Confocal laser imaging showed that pEGFP-C2-L539fs/47 protein was successfully expressed in cytoplasm and cytomembrane of HEK293 cells.Conclusion:pEGFP-C2-L539fs/47 containing the HERG gene mutant was successfully constructed by double digestion method and expressed fusion protein in HEK293 cells,which laid a foundation for the further study on L539fs/47. 展开更多
关键词 HERG gene Nonsense mutations Eukaryotic expression vector PEGFP HEK293 cells
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Effect of Ermiao Fang with Xixin(Herba Asari Mandshurici) on bone marrow stem cell directional homing to a focal zone in an osteoarthritis rat model 被引量:3
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作者 Ying Xu Guojing Dai +5 位作者 Qian Liu Hongwei Zhu Weiheng Chen Ping Zhang Tiejun Zhao Na Lin 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2014年第4期477-487,共11页
OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induc... OBJECTIVE: To investigate the effects of Ermiao Fang(EM) with medical guide Xixin(Herba Asari Mandshurici)(HAM) on bone marrow stem cell migration to a focal zone in osteoarthritis(OA) rats.METHODS: OA rats were induced by arthrectomy and assigned to sham-operated, model, EM, or EM plus HAM groups.All rats were injected with recombinant human granulocyte colony-stimulating factor 30μg·kg-1·d-1for7 days and treated with EMor EM plus HAM at 1.6 or 1.9 g·kg-1·d-1 for 3 or 6 weeks, respectively. Chondrocyte apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Levels of interleukin-1 beta(IL-1β) tumor necrosis factor alpha(TNF-α) nitric oxide(NO), and inducible nitric oxide synthase(iNOS) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay. Matrix metalloproteinases(MMPs)-13,tissue inhibitors of metalloproteinases(TIMPs)-1,Bromodeoxyuridine(BrdU), cluster of differentiation 34(CD34), and stromal cell-derived factor 1(SDF-1) were measured by immunohistochemical assay.RESULTS:The EM and EM plus HAM groups had significantly less cartilage damage and synovium inflammation the model group. Moreover, the EM and EM plus HAM groups had less chondrocyte apoptosis and more proteoglycan and collagen content than the model group.The EM and EMplus HAM groups had obviously higher MMPs-13 and TIMPs-1 expression in the cartilage than the model group. Moreover, the two formula groups had less release of IL-1β, TNF-α, NO, and iNOS than model group. Importantly, the expressions of BrdU, CD34,and SDF-1 in cartilage were significantly higher in the EM and EM plus HAM-Medtreated rats than model group. Notably, the EM plus HAM treatment seemed to have the greatest effects.CONCLUSION: HAM improves the therapeutic effects of EM on OA rats by enhancing BMSC directional homing to the focal zone. 展开更多
关键词 Osteoarthritis Myeloid progenitor cells Homing Ermiao Fang Xixin(Herba Asari Mandshurici)
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Cytotoxicity of mitochondrial-targeting silica-coated manganese oxide nanoparticles
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作者 Jie Wei Chao Yu +4 位作者 Li Wang Jun Wang Zhiguo Zhou Hong Yang Shiping Yang 《Science China Chemistry》 SCIE EI CAS CSCD 2015年第10期1537-1543,共7页
The mitochondrion is a promising target for diagnosis and therapy. Mitochondrial-targeting silica-coated manganese oxide nanoparticles(Mn O@Si O2-PPh3+ NPs) were successfully synthesized to explore the mitochondrial c... The mitochondrion is a promising target for diagnosis and therapy. Mitochondrial-targeting silica-coated manganese oxide nanoparticles(Mn O@Si O2-PPh3+ NPs) were successfully synthesized to explore the mitochondrial cytotoxicity of nanoparticles. The mitochondrial targeting property was confirmed by a laser scanning confocal microscopy experiment. Even after incubation for only 4 h, the cytotoxicity of Mn O@Si O2-PPh3+ NPs against cancer cells was obvious; the ATP content was significantly decreased to 40%; and the mitochondrial membrane potential was depleted. All of these results indicated the collapse of mitochondrial function and the start of a cell apoptosis pathway. Our findings suggest that mitochondrial-mediated apoptosis could be strengthened by targeting to the subcellular compartment. 展开更多
关键词 mitochondrial targeting CYTOTOXICITY APOPTOSIS ATP content mitochondrial membrane potential
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