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中药复方对大鼠肾小球系膜细胞增殖影响的实验研究 被引量:2
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作者 顾江萍 张岩 +1 位作者 梁鑫淼 徐青 《中国中医药科技》 CAS 2006年第2期77-77,共1页
关键词 肾小球系膜细胞增殖 细胞:大鼠 中药复方 二甲基亚砜(DMSO) 实验研 乙二胺四乙酸二钠 L-谷氨酰胺 药品检验所 Gibco 新生牛血清
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内皮祖细胞自体移植联合阿托伐他汀治疗大鼠急性心肌梗死效果观察 被引量:1
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作者 梁鹍 牛颖 魏经汉 《郑州大学学报(医学版)》 CAS 北大核心 2009年第6期1197-1201,共5页
目的:探讨内皮祖细胞(EPCs)自体移植联合阿托伐他汀治疗对急性心肌梗死(AMI)大鼠心功能的影响。方法:30只SD大鼠随机分为心肌梗死对照组(组Ⅰ)、单纯EPCs移植组(组Ⅱ)和阿托伐他汀联合EPCs移植组(组Ⅲ),每组10只。大鼠无菌心脏采血,应... 目的:探讨内皮祖细胞(EPCs)自体移植联合阿托伐他汀治疗对急性心肌梗死(AMI)大鼠心功能的影响。方法:30只SD大鼠随机分为心肌梗死对照组(组Ⅰ)、单纯EPCs移植组(组Ⅱ)和阿托伐他汀联合EPCs移植组(组Ⅲ),每组10只。大鼠无菌心脏采血,应用密度梯度离心法分离单个核细胞,体外培养,获得较纯的EPCs。3组大鼠均结扎左冠状动脉前降支,制造大鼠AMI模型。组Ⅱ、组Ⅲ在模型制备后,于梗死周边区注射5-溴-2’-脱氧尿嘧啶核苷(BrdU)标记的EPCs,组Ⅰ注射等量培养液。24h后,组Ⅲ以阿托伐他汀50mg/(kg.d)灌胃。8周后观察大鼠心功能及血流动力学指标的改变,并以免疫组化方法检测心肌组织内EPCs及修复情况。结果:8周后,3组左室短轴缩短率、射血分数、左室收缩压、左室舒张末压及压力变化速率最大值比较,差异均有统计学意义(F分别为95.7、296.5、844.9、67.6、127.0,P均<0.001)。组Ⅲ大鼠心功能及血流动力学指标较组Ⅱ和组Ⅰ改善(P均<0.05),缺血区域的移植细胞部分分化为血管内皮细胞。组Ⅰ大鼠心肌组织结构紊乱,心肌细胞被瘢痕组织替代;组Ⅱ和组Ⅲ心肌结构排列明显有序。结论:EPCs移植联合阿托伐他汀治疗AMI效果优于单纯EPCs移植。 展开更多
关键词 内皮祖细胞 急性心肌梗死 阿托伐他汀 细胞移植:大鼠
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注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤细胞增殖与氧化损伤的影响 被引量:2
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作者 蒋玉辉 梁蔚阳 《中国医药导报》 CAS 2011年第10期30-31,共2页
目的:探讨注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤细胞的增殖与过氧化氢氧化损伤的影响。方法:采用MTT法,观察不同浓度的注射用脑蛋白水解物对体外培养的大鼠肾上腺嗜铬瘤细胞(PC12)生长及过氧化氢损伤保护的作用。结果:注射用脑蛋... 目的:探讨注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤细胞的增殖与过氧化氢氧化损伤的影响。方法:采用MTT法,观察不同浓度的注射用脑蛋白水解物对体外培养的大鼠肾上腺嗜铬瘤细胞(PC12)生长及过氧化氢损伤保护的作用。结果:注射用脑蛋白水解物对PC12细胞的增殖及氧化损伤的保护作用存在量效关系。结论:注射用脑蛋白水解物可促进PC12细胞生长,并可保护细胞减轻过氧化氢的损伤。 展开更多
关键词 注射用脑蛋白水解物:大鼠肾上腺嗜铬瘤细胞 MTT
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适合膜片钳实验的心肌细胞分离方法研究 被引量:4
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作者 赵临 汪玲芳 +3 位作者 尹永强 吴红 康毅 娄建石 《天津医科大学学报》 2010年第1期12-14,共3页
目的:摸索并建立简便可行的适于做膜片钳实验的心肌细胞急性分离方法。方法:采用自行改进的Langendorff心脏灌流装置,用无钙台氏液和酶解液逆行主动脉灌流之后,剪取心肌组织置于KB液中保存以供全细胞膜片钳方式记录电流。结果:分离所得... 目的:摸索并建立简便可行的适于做膜片钳实验的心肌细胞急性分离方法。方法:采用自行改进的Langendorff心脏灌流装置,用无钙台氏液和酶解液逆行主动脉灌流之后,剪取心肌组织置于KB液中保存以供全细胞膜片钳方式记录电流。结果:分离所得80%~90%的细胞呈杆状,边缘清楚,表面光滑,纹理清晰,可记录到典型的快钠电流。结论:控制好分离心肌细胞的实验条件以及熟练掌握实验操作的方法,是分离出适合于膜片钳实验的心肌细胞的重要因素。 展开更多
关键词 膜片钳:大鼠:心肌细胞:细胞分离
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不同剂量维生素E对去甲肾上腺素诱导的心肌细胞肥大的影响
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作者 陈吉 吴强 +4 位作者 韩烨 郭小平 陈敏 蔡运昌 黄岚 《贵阳医学院学报》 CAS 2004年第1期18-20,24,共4页
目的 :观察不同剂量维生素E对去甲肾上腺素 (NE)诱导心肌细胞肥大的影响。方法 :培养乳鼠心肌细胞暴露于NE(2 0 μmol/L)和维生素E(5 μmol/L ,5 0 μmol/L ,5 0 0 μmol/L) 36h ,检测心肌细胞3 H 亮氨酸掺入率和蛋白质含量的变化。结果... 目的 :观察不同剂量维生素E对去甲肾上腺素 (NE)诱导心肌细胞肥大的影响。方法 :培养乳鼠心肌细胞暴露于NE(2 0 μmol/L)和维生素E(5 μmol/L ,5 0 μmol/L ,5 0 0 μmol/L) 36h ,检测心肌细胞3 H 亮氨酸掺入率和蛋白质含量的变化。结果 :NE可导致心肌细胞3 H 亮氨酸掺入率和蛋白质含量明显增高 ,5 0 μmol/L ,5 0 0 μmol/L的维生素E干预可抑制NE的致心肌细胞肥大的作用。结论 :NE的促心肌细胞肥大作用可能与活性氧生成增加有关 。 展开更多
关键词 维生素E 去甲肾上腺素 肥大 心肌 细胞:大鼠
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Targeted migration and differentiation of engrafted neural precursor cells in amyloid β-treated hippocampus in rats 被引量:1
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作者 唐军 徐海伟 +4 位作者 范晓棠 李志方 李达兵 杨丽 周光纪 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第5期263-270,共8页
Objective To observe the migration and differentiation of the neural precursor cells (NPCs) that derived from murine embryonic stem cells (ESCs) when they were transplanted into amyloid β (Aβ)-treated rat hipp... Objective To observe the migration and differentiation of the neural precursor cells (NPCs) that derived from murine embryonic stem cells (ESCs) when they were transplanted into amyloid β (Aβ)-treated rat hippocampus. Methods MESPU35, a murine ESC cell line that express the enhanced green fluorescent protein (EGFP), was induced differentiation into nestin-positive NPCs by modified serum-free methods. The Aβ plaques and the differentiation of the grafted cells were observed by immunofluorescent staining. Results Comparing 16 weeks with 4 weeks post-transplantation, the migration distance increased about 5 times; the rate of migratory NPCs differentiating into glial fibrillary acidic protein (GFAP)-positive cells kept rising from (30.41 ± 1.45)% to (49.25± 1.23)%, and the rate of NPCs differentiating into neurofilament 200 (NF200) positive cells increased from (16.68±0.95)% to (27.94± 1.21)%. Meanwhile, the GFAP-positive cells targeting to the ipsilateral side of Aβ plaques increased from 60.2% to 81.3 %, while the NF200-positive cells increased from 61.3% to 84.1%. The migration distance had significant positive linear correlations to the neuronal differentiation rate (r = 0.991) and to the astrocytic differentiation rate (r = 0.953). Conclusion Engrafted NPCs migrate targetedly to the Aβ injection site and differentiate into neurons and astrocytes. 展开更多
关键词 embryonic stem cells amyloid β peptide cell transplantation DIFFERENTIATION MIGRATION rat
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Isolation of Ca^(2+) Tolerant Cardiomyocytes from Aadult Rats for Patch Clamp Studies 被引量:2
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作者 徐华娥 陶金 +2 位作者 陈洁 汪红仪 李胜男 《Journal of Nanjing Medical University》 2004年第1期4-6,共3页
Objective: To investigate the factors affecting the viability and Ca 2+ tolerance of isolated rats' cardiac myocytes for patch clamp research. Methods: Hearts were firstly perfused by the Langendorff perfusion ... Objective: To investigate the factors affecting the viability and Ca 2+ tolerance of isolated rats' cardiac myocytes for patch clamp research. Methods: Hearts were firstly perfused by the Langendorff perfusion apparatus with normal Tyrode's solution, then with Ca 2+-free Tyrode's solution and subsequently with low Ca 2+ enzyme solution containing collagenase 0.1-0.2 g/L. All the solutions were saturated with oxygen and the perfusion temperature was kept at 37 ℃. Finally hearts were washed by Ca 2+-free Tyrode's solution, after which the ventricles were minced into small pieces in KB solution, dispersed and filtered. The isolated myocytes were stored in KB solution at room temperature for 1 h and recovered to normal calcium concentration before patch clamp experiments.Results: When all the factors such as water, enzyme, Ca 2+,pH, and oxygen were well controlled, the well constructed and rod-like cardiac myocytes with a yielding rate of 30%-50% came out.Conclusion: All the factors should be well controlled, which ensured the isolated cells Ca 2+ tolerant and appropriate for patch clamp experiments. 展开更多
关键词 ISOLATION cardiac myocyte PATCH-CLAMP Tyrode
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Morphological Characteristics of Smooth Muscle Cells Isolated from the Rat Ductus Deferens
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作者 聂红 尹震 +3 位作者 林倩旋 冯雪莹 张建宇 李孔燕 《Zoological Research》 CAS CSCD 北大核心 2008年第6期633-636,共4页
The aim of this study was to establish a method of isolating and culturing smooth muscle cells from the ductus deferens of rats. Smooth muscle cells were prepared from ductus deferens by explanting technique after dis... The aim of this study was to establish a method of isolating and culturing smooth muscle cells from the ductus deferens of rats. Smooth muscle cells were prepared from ductus deferens by explanting technique after dissection of adventitia and intimae, and cultured in vitro. The identification of the smooth muscle cells were verified by using anti u-smooth muscle actin (a-SMA) immunohistochemistry studies. The result suggested that the cells are multi-morphous, showing long fusiform or star shapes. The apophysis of cells contacted and coalesced to each other, in some regions the cells overlapped in multilayer, while in the other regions they formed monolayer that fluctuated and showed a "peak-valley" shape. They presented a positive reaction through immunohistochemistry studies. The purity of the cells was more than 99% through this method. The culturing of smooth muscle cells by explanting technique is simple and stable. 展开更多
关键词 Ductus deferens smooth muscle cells Cell culture RAT
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Urocortin, the neuropeptide, inhibits the viability of ECV304 cells and rat vascular smooth muscle cells
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作者 陈洁 汪红仪 +3 位作者 陶金 徐华娥 杨荣 李胜男 《Journal of Nanjing Medical University》 2004年第1期1-3,共3页
Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' t... Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 cells and VSMC by using a tetrazolium (MTT) assay.Results: Ucn (10 -7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively(P<0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC. Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling (VR). 展开更多
关键词 UROCORTIN ECV304 vascular smooth muscle cells MTT assay ATP-sensitive potassium channels
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EFFECTS OF ELECTROACUPUNCTURE ON EXPRESSION OF INTERCELLULAR ADHESION MOLECULE-1 IN THE RAT OF LOCAL CEREBRAL ISCHEMIA-REPERFUSION 被引量:2
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作者 孔立红 毛庆菊 陈邦国 《World Journal of Acupuncture-Moxibustion》 2007年第1期31-36,共6页
Objective To investigate effects of electroacupuncture (EA) on expression of intercellular adhesion molecule-1 (ICAM-1) in the rat of local cerebral ischemia-reperfusion. Methods Eighty SD rats were randomly divid... Objective To investigate effects of electroacupuncture (EA) on expression of intercellular adhesion molecule-1 (ICAM-1) in the rat of local cerebral ischemia-reperfusion. Methods Eighty SD rats were randomly divided into a normal control group, a sham operation group, a model group and an EA treatment group, 20 rats in each group. The thread-obstruction method was used for preparation of ischemia-reperfusion model. Zea-Longa rating criteria were used for evaluation of nervous function disorder; Immunohistochemical SABC method was used for detection of ICAM-1 expression in the microvascular endothelial cell of the ischemic brain region, and ELISA method for the soluble ICAM-1 (slCAM-1) content in peripheral blood. Re. suits After cerebral ischemia-reperfusion, both ICAM-1 expression level in the microvascular endethelium cell of the ischemic brain region and slCAM-1 content in the peripheral blood significantly increased in the model group as compared with the normal group and the sham operation group (P〈0.01); After EA treatment, the ICAM-1 expression level in the microvascular endothelial cell of the ischemic brain region and slCAM-1 content in the peripheral blood were significantly down-regulated in the EA treatment group as com- pared with the model group (P〈 0.05). Conclusion After cerebral ischemia-reperfusion, the microvascular endothelial cell of the ischemic brain region releases ICAM-1, which induces inflammatory injury of cerebral tissues; EA treatment can decease the expression of ICAM-1, so as to prevent the brain from the injury. 展开更多
关键词 Electroacupuncture Cerebral ischemia and reperfusion Intercellularadhesion molecule-1 (I-CAM-1 Soluble intercellular adhesion molecule-1 (slCAM-1)
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Antineoplastic Effect of Calcium Channel Blocker-Verapamil and 5-Fluorouracil Intraperitoneal Chemotherapy on Hepatocarcinoma-Bearing Rats
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作者 曹天生 史海安 周亚魁 《The Chinese-German Journal of Clinical Oncology》 CAS 2002年第2期84-87,共4页
Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy onhepatocarcinoma-bearing rats, and examine the action between calcium channel bloc... Objective To study the antineoplastic effect of the calcium channel blocker verapamil and 5-fluorouracil intraperitoneal chemotherapy onhepatocarcinoma-bearing rats, and examine the action between calcium channel blockers and cytotoxic drugs.Methods We adopted the method of subcapsular implantation of carcinoma tissues of walker-256 in the left liver lobe as a model of livercarcinoma-bearing rats. All experimental animals were divided into four groups. On the sixth day post implantation, in group A (controlgroup) 6 ml of saline was injected intraperitoneally once a day for 3 days. In group B (single chemotherapy group) 6 ml of 5-Fu 75 mg/kg was injected intraperitoneally once a day for 3 days. In group C (combination of treatment group) both 5-Fu (75 mg/kg) and verapamil(25 mg/kg) were administered simultaneously as in A and B. In group D (simple verapamil group) only 6 ml of verapamil (25 mg/kg)was administered as above.Results Compared with groups A, B and D, The volume of cancer and the contents of liver cancer DNA and protein were significantlyreduced. The rates of inhibiting cancer (89.9% in group C and 35.4% in group B) were significantly increased in group C. Group C hadsignificantly long survival time compared to groups A, B and D ( P < 0.05) . By light microscopy, a number of focal necroses were foundin cancer tissue in group C.Conclusion Calcium channel blockers can enhance the antineoplastic effect of 5-Fu intraperitoneal chemotherapy to liver cancer ; Theuse of verapamil can not increase the toxicity of 5-Fu. 展开更多
关键词 calcium channel blockers VERAPAMIL 5-FLUOROURACIL HEPATOCARCINOMA intraperitoneal chemotherapy
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Effects of V2O5 on Proliferation and Differentiation of Limb Bud Cells of Rat
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作者 张天宝 孙棉龄 《Developmental and Reproductive Biology》 1997年第2期39-45,共7页
The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious i... The micromass culture was used to determine the effects of vanadium pentoxide (V2O5 ) on the proliferation and differentiation of limb bud cells of rat. In the in vitro test, the results showed that V2O5 had obvious inhibiting effects on both proliferation and differentiation of limb bud cells with a dosedependent response, its proliferating and differentiating IC50 being 13.64 and 4.77μmol/L, respectively. In the in vivo/in vitro test, the results showed that V2O5 had no obvious effect on cell proliferation but had obvious inhibiting effect on cell differentiation. These results indicated that V2O5 might have a specific inhibiting effect on the differentiation of limb bud cells. 展开更多
关键词 micromass culture limb bud cells developmental toxicity V2O5 rat.
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Mechanical stretch induces mitochondria-dependent apoptosis in neonatal rat cardiomyocytes and G_(2)/M accumulation in cardiac fibroblasts 被引量:6
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作者 XuDongLIAO XiaoHuiWANG +2 位作者 HaiJingJIN LanYingCHEN QuanCHEN 《Cell Research》 SCIE CAS CSCD 2004年第1期16-26,共11页
Heart remodeling is associated with the loss of cardiomyocytes and increase of fibrous tissue owing to abnormal mechanical load in a number of heart disease conditions. In present study, a well-described in vitro sust... Heart remodeling is associated with the loss of cardiomyocytes and increase of fibrous tissue owing to abnormal mechanical load in a number of heart disease conditions. In present study, a well-described in vitro sustained stretch model was employed to study mechanical stretch-induced responses in both neonatal cardiomyocytes and cardiac fibroblasts. Cardiomyocytes, but not cardiac fibroblasts, underwent mitochondria-dependent apoptosis as evidenced by cytochrome c (cyto c) and Smac/DIABLO release from mitochondria into cytosol accompanied by mitochondrial membrane potential (△ψ_m) reduction, indicative of mitochondrial permeability transition pore (PTP) opening. Cyclosporin A, an inhibitor of PTP, inhibited stretch-induced cyto c release, △ψ_m reduction and apoptosis, suggesting an important role of mitochondrial PTP in stretch-induced apoptosis. The stretch also resulted in increased expression of the pro-apoptotic Bcl-2 family proteins, including Bax and Bad, in cardiomyocytes, but not in fibroblasts. Bax was accumulated in mitochondria following stretch. Cell permeable Bid-BH3 peptide could induce and facilitate stretch-induced apoptosis and △ψ_m reduction in cardiomyocytes. These results suggest that Bcl-2 family proteins play an important role in coupling stretch signaling to mitochondrial death machinery, probably by targeting to PTP. Interestingly, the levels of p53 were increased at 12 h after stretch although we observed that Bax upregulation and apoptosis occurred as early as 1 h. Adenovirus delivered dominant negative p53 blocked Bax upregulation in cardiomyocytes but showed partial effect on preventing stretch-induced apoptosis, suggesting that p53 was only partially involved in mediating stretch-induced apoptosis. Furthermore, we showed that p21 was upregulated and cyclin B1 was downregulated only in cardiac fibroblasts, which may be associated with G_2/M accumulation in response to mechanical stretch. 展开更多
关键词 APOPTOSIS mechanical stretch Bcl-2 and its family proteins MITOCHONDRIA cardiomyocyte.
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Fentanyl inhibits glucose-stimulated insulin release from β-cells in rat pancreatic islets 被引量:6
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作者 Tao-Lai Qian Xin-Hua Wang +2 位作者 Sheng Liu Liang Ma Ying Lu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第33期4163-4169,共7页
AIM: TO explore the effects of fentanyl on insulin release from freshly isolated rat pancreatic islets in static culture. METHODS: Islets were isolated from the pancreas of mature Sprague Dawley rats by common bile ... AIM: TO explore the effects of fentanyl on insulin release from freshly isolated rat pancreatic islets in static culture. METHODS: Islets were isolated from the pancreas of mature Sprague Dawley rats by common bile duct intraductal collagenase V digestion and were purified by discontinuous Ficoll density gradient centrifugation. The islets were divided into four groups according to the fentanyl concentration: control group (0 ng/mL), group I (0.3 ng/mL), group I (3.0 ng/mL), and group III (30 ng/mL). In each group, the islets were co-cultured for 48 h with drugs under static conditions with fentanyl alone, fentanyl + 0.1 μg/mL naloxone or fentanyl + 1.0 μg/mL naloxone. Cell viability was assessed by the MTT assay. Insulin release in response to low and high concentrations (2.8 mmol/L and 16.7 mmol/L, respectively) of glucose was investigated and electron microscopy morphological assessment was performed. RESULTS: Low- and high-glucose-stimulated insulin release in the control group was significantly higher than in groups I and II (62.33 ± 9.67 μIU vs 47.75 ± 8.47 μIU, 39.67 ± 6.18 μIU and 125.5 ± 22.04 μIU vs 96.17 ± 14.17 μIU, 75.17 ± 13.57 μIU, respectively, P 〈 0.01) and was lowest in group III (P 〈 0.01). After adding 1 μg/mL naloxone, insulin release in groups II and II was not different from the control group. Electron microscopy studies showed that the islets were damaged by 30 ng/ml fentanyl. CONCLUSION: Fentanyl inhibited glucose-stimulated insulin release from rat islets, which could be prevented by naloxone. Higher concentrations of fentanyl significantly damaged β-cells of rat islets. 展开更多
关键词 FENTANYL INHIBITION Insulin release ISLETS
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The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras
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作者 郭继彤 李雪峰 +6 位作者 Shahnaz Fida 苟克勉 Nakisa Malakooti ZHANG Chun-fang John R Morrison Alan O Trounson DU Zhong-tao 《Zoological Research》 CAS CSCD 北大核心 2009年第2期158-164,共7页
The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In ... The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In result, three rat chimeras were produced by day 5 (D5) Sprague-Dawley (SD) blastocysts injected with ICMs derived from day 6 (D6) and D5 Dark Agouti (DA) blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs arc able to contribute to chimeras, but their potential decreases significantly after culture in vitro (P〈0.05), and rFNS cells only have the potential to contribute to early fetal development. 展开更多
关键词 Rat chimeras Inner cell mass Rat fetal neural stem cells Blastocyst injection
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Neurogenesis by Activation of Inherent Neural Stem Cells in the Rat Hippocampus after Cerebral Infarction 被引量:14
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作者 Bo Zhang Ren-zhi wang +2 位作者 Zhi-gang Lian Yang Song Yong Yao 《Chinese Medical Sciences Journal》 CAS CSCD 2009年第1期41-45,共5页
Objective To investigate the changes of neural stem cells (NSCs) in the rat hippocampus after cerebral infarction (CI) and to evaluate the neurogenesis caused by the activation of NSCs. Methods CI models of rats were ... Objective To investigate the changes of neural stem cells (NSCs) in the rat hippocampus after cerebral infarction (CI) and to evaluate the neurogenesis caused by the activation of NSCs. Methods CI models of rats were made and rats were assigned to 6 groups: sham-operated, 1 day, 3 days, 7 days, 14 days, and 28 days after CI. The dynamic expression of bromodeoxyuridine (BrdU), polysialylated neural cell adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP), and neuronal nuclear antigen (NeuN) were determined by immunohistochemistry and immunofluorescence staining. BrdU was used to mark the proliferated NSCs. PSA-NCAM was used to mark the plasticity of activated NSCs. GFAP and NeuN were used to mark the differentiated NSCs. Results Compared with the controls, the number of BrdU+ cells in the hippocampus increased significantly at 1 day after CI (P<0.05), reached peak at 7 days after CI (P<0.05), decreased but still elevated compared with the controls at 14 days after CI (P<0.05), and nearly unchanged at 28 days after CI. The number of BrdU+/PSA-NCAM+ cells increased significantly at 7 days after CI (P<0.05), reached peak at 14 days after CI (P<0.05), and decreased but still elevated compared with the controls at 28 days after CI (P<0.05). The number of BrdU+/PSA-NCAM+ cells was equal to 60% of the number of BrdU+ cells in all the same period. The number of BrdU+/NeuN+ cells in the hippocampus increased significantly at 14 days after CI (P<0.05) and reached peak at 28 day after CI (P<0.05). The number of BrdU+/GFAP+cells in the hippocampus nearly unchanged after CI. Conclusion CI can stimulate the proliferation of inherent NSCs, and most proliferated NSCs may differentiate into neurons and represent neural plasticity. 展开更多
关键词 cerebral infarction neural stem cells NEUROGENESIS HIPPOCAMPUS
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Correlation between anti-fibrotic effect of baicalin and serum cytokines in rat hepatic fibrosis 被引量:28
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作者 Xiao-Dong Peng Li-Li Dai +2 位作者 Chang-Quan Huang Chun-Mei He Li-Juan Chen 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第37期4720-4725,共6页
AIM: To investigate the correlation between the antifibrotic effect of baicalin and serum cytokine production in rat hepatic fibrosis, METHODS: Forty male Sprague-Dawley rats were divided randomly into four groups:... AIM: To investigate the correlation between the antifibrotic effect of baicalin and serum cytokine production in rat hepatic fibrosis, METHODS: Forty male Sprague-Dawley rats were divided randomly into four groups: normal control group, model group, baicalin-treated group, and colchicine-treated group. Except for the normal control group, all rats in the other groups were administered with carbon tetrachloride to induce hepatic fibrosis. At the same time, the last two groups were also treated with baicalin or colchicine. At the end of the 8 wk, all animals were sacrificed. Serum alanine aminotransferase (ALl'), aspartate aminotransferase (AST), transforming growth factor (TGF)-β1, tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-10 were measured. Liver index, hepatic hydroxyproline content and the degree of liver fibrosis were also evaluated. RESULTS: The levels of ALT, AST and liver index in the baicalin-treated group were markedly lower than those in the model group (ALT: 143.88 ± 14.55 U/L vs 193.58± 24.35 U/L; AST: 263.66 ± 44.23 U/L vs 404.37± 68.29 U/L; liver index: 0.033 ± 0.005 vs 0.049± 0.009, P 〈 0.01). Baicalin therapy also significantly attenuated the degree of hepatic fibrosis, collagen area and collagen area percentage in liver tissue (P 〈 0.01). Furthermore, the levels of serum TGF-β1, TNF-α and IL-6 were strikingly reduced in the baicalin-treated group compared with the model group, while the production of IL-10 was up-regulated: (TGF-β1:260.21 ± 31.01 pg/mL vs 375.49 ± 57.47 pg/mL; TNF-α: 193.40±15.18 pg/mL vs 260.04 ± 37.70 pg/mL; IL-α:339.87 ± 72.95 pg/mL vs 606.47 ± 130.73 pg/mL; IL-10:506.22 ± 112.07 pg/mL vs 316.95 ± 62.74 pg/mL, P 〈 0.01). CONCLUSION: Baicalin shows certain therapeutic effects on hepatic fibrosis, probably by immunoregulating the imbalance between profibrotic and antifibrotic cytokines. 展开更多
关键词 BAICALIN Hepatic fibrosis Hepatic stellate cell CYTOKINES
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Cognitive improvement following transvenous adipose-derived mesenchymal stem cell transplantation in a rat model of traumatic brain injury 被引量:7
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作者 Dongfei Li Chun Yang Rongmei Qu Huiying Yang Meichun Yu Hui Tao Jingxing Dai Lin Yuan 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第10期732-737,共6页
The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model... The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model of traumatic brain injury following ADMSC transplantation via the tail vein. Cell transplants were observed in injured cerebral cortex, and expression of brain-derived nerve growth factor was significantly increased in the injured hippocampus following transplantation. Results demonstrated that transvenous ADMSC transplants migrated to the injured cerebral cortex and significantly improved cognitive function. 展开更多
关键词 adipose-derived mesenchymal stem cells traumatic brain injury brain-derived nerve growth factor CORTEX stem cell transplantation neural regeneration
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Moxibustion activates mast cell degranulation at the ST25 in rats with colitis 被引量:4
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作者 Yin Shi Li Qi +4 位作者 Jing Wang Ming-Shu Xu Dan Zhang Lu-Yi Wu Huan-Gan Wu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第32期3733-3738,共6页
AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Spragu... AIM: To investigate the effects of moxibustion on the morphology and function of mast cells (MC) at Tianshu (ST25) in rats with trinitro-benzene-sulfonic acid (TNBS)-induced colitis. METHODS: A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxi-bustion group, moxibustion plus disodium cromoglycate (M + DC) group and moxibustion plus normal saline (M+ NS) group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M + DC and M + NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxy- lin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas. RESULTS: An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group (45.91 ± 11.41 vs 32.58 ± 8.28, P < 0.05). After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed. CONCLUSION: Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint. 展开更多
关键词 Disodium cromoglycate COLITIS Mast cell MOXIBUSTION ST25 acupoint
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Prunella vulgaris L. extract improves cellular immunity in MDR-TB challenged rats 被引量:3
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作者 Lu Jun Qin Rui +1 位作者 Ye Song Yang Mei 《Journal of Medical Colleges of PLA(China)》 CAS 2011年第4期230-237,共8页
Objective: To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis (MDR-TB). Methods: Experimental animal model in rats was induced by MDR-TB. Normal group mode... Objective: To study the effect of the extract of Prunella vulgaris L. on multiple drugs resistant bacillus tuberculosis (MDR-TB). Methods: Experimental animal model in rats was induced by MDR-TB. Normal group model group and Prunella vulgaris L. group were set up. The contents of IFN-7, IL-4, IL-10 and IL-12 were examined by ELISA. Their genome mRNAs were extracted, the target genes were amplified by PCR. RT-PCR was used to detect the mRNA levels of them. Results: The content of IFN-q, of the extract of Prunella vulgaris L. group was 1.98±0.67 pg/ml, IL-4 was 6.47±1.46 pg/ml, IL-10 was 12.13±3.43 pg/ml and IL-12 was 3.02±0.86 pg/ml. Compared with the model group, Prunella vulgaris L. group was notable difference in serum IFN-γ, IL-12 and IL-10 (P〈0.05). The mRNA levels of IFN-γ, IL-12 increased and IL-10 decreased obviously, the differences were quite significant (P〈0.05), but IL-4 had no obvious change. Conclusion: The extract of Prunella vulgaris L. can enhance the cellar immunological function in rats from up-regulation of the level of genetic transcription, accordingly provide the theory basis of healing of tuberculosis with it. 展开更多
关键词 Prunella vulgaris L. EXTRACT Multiple drugs resistant bacillus tuberculosi CYTOKINE Immunological function RATS
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