Both NOD2 (CARD15) alleles aremutated in roughly 15%of patients with Crohn’s disease, but functional effects are unclear. We analysed the cytokine response of peripheral blood mononuclear cells to muramyl dipeptide (...Both NOD2 (CARD15) alleles aremutated in roughly 15%of patients with Crohn’s disease, but functional effects are unclear. We analysed the cytokine response of peripheral blood mononuclear cells to muramyl dipeptide (MDP), the ligand for NOD2. MDP induced little TNFαor interleukin 1β, but strong interleukin-8 secretion. MDP also substantially upregulated sec retion of TNFαand interleukin 1βinduced by toll-like receptor ligands. These effects were abolished by the most common Crohn’s NOD2 double mutant genotypes at low nanomolar MDP concentrations, and provide the basis to develop a test of NOD2 functional deficiency. In Crohn’s disease, there are defects in neutrophil recruitment driven by NOD2 and interleukin 8 and in cross talk between the NO D2 and toll-like receptor pathways, which suggests that the immune system fails to receive an early priming signal.展开更多
为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后T...为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后TLR2、TLR4、CD16mRNA的转录水平动态变化基本相似,3、7d高于对照组,14d低于对照组,28d接近对照组,且3d时TLR4、CD16差异极显著(P<0.01),14d仅TLR2差异显著(P<0.05);CD18mRNA的转录水平,3d时显著低于对照组(P<0.05),而7d时显著高于对照组(P<0.05),28d接近正常。以上结果表明,PCV2感染PAM后可引起PAM对异物识别吞噬功能与吞噬后信号转导功能出现紊乱,影响了PAM吞噬和清除病原微生物的能力。展开更多
文摘Both NOD2 (CARD15) alleles aremutated in roughly 15%of patients with Crohn’s disease, but functional effects are unclear. We analysed the cytokine response of peripheral blood mononuclear cells to muramyl dipeptide (MDP), the ligand for NOD2. MDP induced little TNFαor interleukin 1β, but strong interleukin-8 secretion. MDP also substantially upregulated sec retion of TNFαand interleukin 1βinduced by toll-like receptor ligands. These effects were abolished by the most common Crohn’s NOD2 double mutant genotypes at low nanomolar MDP concentrations, and provide the basis to develop a test of NOD2 functional deficiency. In Crohn’s disease, there are defects in neutrophil recruitment driven by NOD2 and interleukin 8 and in cross talk between the NO D2 and toll-like receptor pathways, which suggests that the immune system fails to receive an early priming signal.
文摘为了解猪圆环病毒2型(porcine circovirus type 2,PCV2)感染对仔猪的肺泡巨噬细胞(PAM)免疫功能造成的影响,试验对PCV2感染40日龄左右健康仔猪PAM模式识别受体TLR2、TLR4、CD16、CD18mRNA的动态变化进行了研究。结果发现,在病毒感染后TLR2、TLR4、CD16mRNA的转录水平动态变化基本相似,3、7d高于对照组,14d低于对照组,28d接近对照组,且3d时TLR4、CD16差异极显著(P<0.01),14d仅TLR2差异显著(P<0.05);CD18mRNA的转录水平,3d时显著低于对照组(P<0.05),而7d时显著高于对照组(P<0.05),28d接近正常。以上结果表明,PCV2感染PAM后可引起PAM对异物识别吞噬功能与吞噬后信号转导功能出现紊乱,影响了PAM吞噬和清除病原微生物的能力。
