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抗微生物药物的细胞内活性
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作者 徐伟 贾志敏 吴曙光 《国外医药(抗生素分册)》 CAS 2000年第2期71-74,共4页
阐述了影响病原体、抗微生物药物进入细胞的因素,具有细胞内活性的抗微生物药物分布与临床疗效以及抗微生物药物的临床应用,从而为临床医生用药提供指导。
关键词 细胞内活性 抗生素
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Effect of Platelet Activation and Endothelial Cell Injury on Malignant Cancer 被引量:1
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作者 孙达春 张爱华 +2 位作者 李小亮 吴红 何志国 《The Chinese-German Journal of Clinical Oncology》 CAS 2003年第4期243-245,254,共4页
Objective: To study the effects of platelet activation and endothelial cell injury on the patients with malignant tumor and their prognoses.Methods: Radioimmunity and ELISA methods were employed to detect the TXB2, GM... Objective: To study the effects of platelet activation and endothelial cell injury on the patients with malignant tumor and their prognoses.Methods: Radioimmunity and ELISA methods were employed to detect the TXB2, GMP-140, vWF, cGMP and FN in 78 cases of malignant tumor and 40 healthy control persons.Results: The levels of TXB2, MP-140 and cGMP were increased in intestinal cancer group, lung cancer group and hepatic cancer group, while FN decreased in intestinal cancer and lung cancer group. cGMP was positively related to TXB2, GMP-140, vWF in malignant tumor group. FN was decreased in the group complicated with infection and the group with metastasis, while the other indexes increased. GMP-140, vWF and cGMP was decreased after operation except for the increasing of FN.Conclusion: Activations of platelet and injury of endothelial cells developed in patients with malignant tumor, and both of them affected the metastasis and prognosis of malignant tumor. Key words platelet activation - epithelium injury - malignant tumor - metastasis This work was supported by grants from Guangdong Medical Science foundation (A2000633). 展开更多
关键词 platelet activation epithelium injury malignant tumor METASTASIS
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Alphastatin downregulates vascular endothelial cells sphingosine kinase activity and suppresses tumor growth in nude mice bearing human gastric cancer xenografts 被引量:7
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作者 Lin Chen Tao Li Rong Li Bo Wei Zheng Peng 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第26期4130-4136,共7页
AIM: To investigate whether alphastatin could inhibit human gastric cancer growth and furthermore whether sphingosine kinase (SPK) activity is involved in this process. METHODS: Using migration assay, MTT assay an... AIM: To investigate whether alphastatin could inhibit human gastric cancer growth and furthermore whether sphingosine kinase (SPK) activity is involved in this process. METHODS: Using migration assay, MTT assay and Matrigel assay, the effect of alphastatin on vascular endothelial cells (ECs) was evaluated in vitro. SPK and endothelial differentiation gene (EDG)-1, -3, -5 mRNAs were detected by reverse transcription-polymerase chain reaction (RT-PCR). SPK activity assay was used to evaluate the effect of alphastatin on ECs. Matrigel plug assay in nude mice was used to investigate the effect of alphastatin on angiogenesis in vivo. Female nude mice were subcutaneously implanted with human gastric cancer cells (BGC823) for the tumor xenografts studies. Micro vessel density was analyzed in Factor Ⅷ-stained tumor sections by the immunohistochemical SP method. RESULTS: In vitro, alphastatin inhibited the migration and tube formation of ECs, but had no effect on proliferation of ECs. RT-PCR analysis demonstrated that ECs expressed SPK and EDG-1, -3, -5 mRNAs. In vivo, alphastatin sufficiently suppressed neovascularization of the tumor in the nude mice. Daily administration of alphastatin produced significant tumor growth suppression. Immunohistochemical studies of tumor tissues revealed decreased micro vessel density in alphastatin-treated animals as compared with controls. CONCLUSION: Downregulating ECs SPK activity may be one of the mechanisms that alphastatin inhibits gastric cancer angiogenesis. Alphastatin might be a useful and relatively nontoxic adjuvant therapy in the treatment of gastric cancer. 展开更多
关键词 Stomach neoplasm Angiogenesis Endothelial cells Sphingosine kinase Cancer therapy
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How to protect liver graft with nitric oxide 被引量:11
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作者 Hassen Ben Abdennebi Mohamed Amine Zaoualí +2 位作者 Izabel Alfany-Fernandez Donia Tabka Joan Roselló-Catafau 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第24期2879-2889,共11页
Organ preservation and ischemia reperfusion injury associated with liver transplantation play an important role in the induction of graft injury. One of the earliest events associated with the reperfusion injury is en... Organ preservation and ischemia reperfusion injury associated with liver transplantation play an important role in the induction of graft injury. One of the earliest events associated with the reperfusion injury is endothelial cell dysfunction. It is generally accepted that endothelial nitric oxide synthase (e-NOS) is cell-pro- tective by mediating vasodilatation, whereas inducible nitric oxide synthase mediates liver graft injury after transplantation. We conducted a critical review of the literature evaluating the potential applications of regulating and promoting e-NOS activity in liver preservation and transplantation, showing the most current evidence to support the concept that enhanced bioavailability of NO derived from e-NOS is detrimental to ameliorate graft liver preservation, as well as preventing subse- quent graft reperfusion injury. This review deals mainly with the beneficial effects of promoting "endogenous" pathways for NO generation, via e-NOS inducer drugs in cold preservation solution, surgical strategies such as ischemic preconditioning, and alternative "exogenous" pathways that focus on the enrichment of cold storage liquid with NO donors. Finally, we also provide a basic bench-to-bed side summary of the liver physiology and cell signalling mechanisms that account for explaining the e-NOS protective effects in liver preservation and transplantation. 展开更多
关键词 Cold ischemia reperfusion injury Endotheli-al nitric oxide synthase Nitric oxide Liver graft preser-vation Ischemic preconditioning Liver transplantation
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急性淋巴细胞白血病患儿亚甲基四氢叶酸还原酶多态性与氨甲蝶呤毒副反应的研究 被引量:26
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作者 余慧 金润铭 +1 位作者 白燕 林雯 《中华儿科杂志》 CAS CSCD 北大核心 2005年第4期302-303,共2页
关键词 氨甲蝶呤 白血病患儿 急性淋巴细胞白血病(ALL) 毒副反应 酶多态性 亚甲基四氢叶酸还原酶 髓外白血病 细胞内活性 MTX 敏感性 维持治疗 常用药物 代谢药物 叶酸代谢 耐受性 抗叶酸 关键酶 机体
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Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract
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作者 徐雯 姚克 +2 位作者 孙朝晖 王凯军 申屠形超 《Journal of Zhejiang University Science》 CSCD 2004年第6期743-748,共6页
Objective: To study the role of calpain in the mechanism of oxidative cataract through detecting the level of intracellular free Ca2+, the expression and proteolytic activity of calpain in the lens epithelial cells (... Objective: To study the role of calpain in the mechanism of oxidative cataract through detecting the level of intracellular free Ca2+, the expression and proteolytic activity of calpain in the lens epithelial cells (LECs) of H2O2-induced cataract. Methods: Rat lenses were cultured in vitro and cataract was induced by H2O2. The level of intracellular free Ca2+ was measured by fluorescence determination with fura-2/AM. The expression of m-calpain protein in LECs was detected with immunohistochemical method. The proteolytic activity in LECs was measured using a fluorogenic synthetic substrate. Results: There were significant differences of the level of intracellular free Ca2+ (P=0.001, 0.000, 0.000), the expression of m-calpain (P=0.001, 0.000, 0.000) and the proteolytic activity of calpain (P=0.001, 0.000, 0.000) between H2O2-induced and control group at 6, 12 and 24 h, respectively. Conclusions: H2O2 can increase intracellular free Ca2+, then enhance the expression and proteolytic activity of calpain which may play a role in the mechanism of oxidative cataract of rat. 展开更多
关键词 CATARACT Lens epithelial cell Hydrogen peroxide CALPAIN CALCIUM
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Gradient decrease in telomerase and its RNA in progressive passage of human endometrial stromal stem cells
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作者 Yang Xinyuan Wang Wei +2 位作者 Wang Yueling Chen Wei Li Xu 《Journal of Medical Colleges of PLA(China)》 CAS 2011年第5期254-263,共10页
The purpose of this study was to explore the change of telomerase in passage from human endometrial stromal stem cells isolated from human endometrium.Telomerase activity of cultured endometrial stromal cells was asse... The purpose of this study was to explore the change of telomerase in passage from human endometrial stromal stem cells isolated from human endometrium.Telomerase activity of cultured endometrial stromal cells was assessed at mRNA and protein levels using RT-PCR and immunohistochemistry technique.Telomerase mRNA and protein levels were higher at early passages,and then had a gradually decreased trend of immunoreactive intensity and gradually weakened positive cells with progressive passage in endometrial stromal stem cells.These results suggest that telomerase is contributed to the insenecence of endometrial stem cell. 展开更多
关键词 Endometrial stromal cell TELOMERASE Cell culture
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hVEGF165 Expression in Escherichia coli Conserves Its Biological Function
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作者 Gabajova Blanka Valkova Danka +3 位作者 Bohac Andrej Kovacova Elena Moravcik Roman Zeman Michal 《Journal of Chemistry and Chemical Engineering》 2012年第8期738-743,共6页
The paper describes the expression of human protein VEGF165 in Escherichia coli and its purification. This growth factor isoform contains exon 7, which is essential for binding to extracellular domain of VEGF receptor... The paper describes the expression of human protein VEGF165 in Escherichia coli and its purification. This growth factor isoform contains exon 7, which is essential for binding to extracellular domain of VEGF receptor 2, located on endothelial cells lining the surface of blood vessels. This binding stimulates the cascade of downstream signalling events leading to process known as angiogenesis, hVEGF165 overexpressed with His-tag in BL21 E. coli cells forms inclusion bodies (insoluble protein), so the research found the procedure for its solubilization and purification on a Nickel based affinity chromatography. Although this eukaryotic signal protein needs posttranslational processing for its full function as a homodimer, author verified the biological activity of our hVEGF165 protein, obtained as monomer, by wound healing test. 展开更多
关键词 VEGFI65 endothelial cells HYPOXIA ANGIOGENESIS inclusion bodies protein purification wound healing test.
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Recent advances in cell imaging and cytotoxicity of intracellular stimuli-responsive nanomaterials 被引量:6
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作者 Wenbo Zhang Changyou Gao 《Science Bulletin》 SCIE EI CAS CSCD 2015年第23期1973-1979,共7页
The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and o... The stimuli-responsive nanomaterials are gaining more and more interest in the biological field,including cell imaging and biosensing etc. Nanomaterials in response to the bio-relevant stimuli(i.e., p H, enzymes and other bioactive molecules) can be utilized to enhance imaging(i.e., optical imaging, MRI, and multi-mode imaging) sensitivity via disease site-specific delivery and controlled release, which helps to diagnose cancer at an early stage or to monitor progression during treatment. In the triggered responsive process, smart nanomaterials undergo changes in physiochemical properties that can cause cytotoxicity or influence on cell functions due to the interactions between nanomaterials and cells. In order to promote the design and fabrication of effective platforms for therapeutics and diagnostics, special attention should be paid to these effects. By taking the advantages of intracellular stimuli, the controlled self-assembly in living cells can be achieved, which has been used for various in situ detections and insights into biological self-assembly. In this review, the recent advances in cell imaging, cytotoxicity and self-assembly of intracellular stimuli-responsive nanomaterials are summarized. Some principles for the further design and applications of intracellular stimuli-responsive nanomaterials and future perspectives are discussed. 展开更多
关键词 Stimuli-responsive · Nanomaterials ·Cell imaging · Cytotoxicity · Self-assembly
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