近年来,传统中医药在临床疾病治疗中应用越来越广泛。中药治疗疾病与细胞关联密切,而细胞内物质环境的变化对中药治疗的过程有重要意义。通过检索Web of Science、Pubmed、万方、知网等数据库的相关文献,搜集并整理有关中药对细胞内pH...近年来,传统中医药在临床疾病治疗中应用越来越广泛。中药治疗疾病与细胞关联密切,而细胞内物质环境的变化对中药治疗的过程有重要意义。通过检索Web of Science、Pubmed、万方、知网等数据库的相关文献,搜集并整理有关中药对细胞内pH值、细胞因子、内质网、线粒体和细胞核作用的研究进展,为阐明中药作用与细胞的关联提供依据。展开更多
横河电机公司宣布已开发出SU10 Single Cellome分析仪,该装置使用纳米移液器在单个细胞的目标位置注入基因和药物等物质,并抽吸细胞内物质。SU10针对生物研究市场,即日起在日本发售,随后将在美国等其他市场发售。通过这些解决方案的开发...横河电机公司宣布已开发出SU10 Single Cellome分析仪,该装置使用纳米移液器在单个细胞的目标位置注入基因和药物等物质,并抽吸细胞内物质。SU10针对生物研究市场,即日起在日本发售,随后将在美国等其他市场发售。通过这些解决方案的开发,横河电机的生命创新业务正在培育以使用智能细胞为核心的产业发展。展开更多
Objective: To investigate the effects of motilin and erythromycin on intracellular Ca2+ mobi-lization in cultured myenteric neurons of rats. Methods: The cultured myenteric neurons were identified with immunofluoresce...Objective: To investigate the effects of motilin and erythromycin on intracellular Ca2+ mobi-lization in cultured myenteric neurons of rats. Methods: The cultured myenteric neurons were identified with immunofluorescence staining technique. Motilin-induced and erythromycin-induced intracellular Ca2+ mobilization was studied in primary cultures of myenteric neurons using the ratiometric Ca2+ indicator Furo3/AM, with a laser confocal microscope. Results: The effects of motilin and erythromycin on intracellular Ca2+ mobilization were as follows: (1)In Hank's solution, 10 -8, 10-7, 10-6 mol/L motilin could elevate intracellular Ca2+ concentration ([Ca2+]i)in a dose-dependent manner. (2) In Hank's solution, 10μg/ ml erythromycin also could induce the elevation of [Ca2+]i. (3) After pretreatment with antibody against the motilin receptor in Hank's solution, the Ca2+ response to erythromycin was almost restricted. Conclusion: It is suggested that motilin could increase [Ca2+]i in myenteric neurons in a dose-dependent manner, and erythromycin may also have this effectivenesss by binding to the motilin receptor.展开更多
文摘近年来,传统中医药在临床疾病治疗中应用越来越广泛。中药治疗疾病与细胞关联密切,而细胞内物质环境的变化对中药治疗的过程有重要意义。通过检索Web of Science、Pubmed、万方、知网等数据库的相关文献,搜集并整理有关中药对细胞内pH值、细胞因子、内质网、线粒体和细胞核作用的研究进展,为阐明中药作用与细胞的关联提供依据。
文摘横河电机公司宣布已开发出SU10 Single Cellome分析仪,该装置使用纳米移液器在单个细胞的目标位置注入基因和药物等物质,并抽吸细胞内物质。SU10针对生物研究市场,即日起在日本发售,随后将在美国等其他市场发售。通过这些解决方案的开发,横河电机的生命创新业务正在培育以使用智能细胞为核心的产业发展。
基金Supported by the National Natural Science Foundation of China (No. 30170414)
文摘Objective: To investigate the effects of motilin and erythromycin on intracellular Ca2+ mobi-lization in cultured myenteric neurons of rats. Methods: The cultured myenteric neurons were identified with immunofluorescence staining technique. Motilin-induced and erythromycin-induced intracellular Ca2+ mobilization was studied in primary cultures of myenteric neurons using the ratiometric Ca2+ indicator Furo3/AM, with a laser confocal microscope. Results: The effects of motilin and erythromycin on intracellular Ca2+ mobilization were as follows: (1)In Hank's solution, 10 -8, 10-7, 10-6 mol/L motilin could elevate intracellular Ca2+ concentration ([Ca2+]i)in a dose-dependent manner. (2) In Hank's solution, 10μg/ ml erythromycin also could induce the elevation of [Ca2+]i. (3) After pretreatment with antibody against the motilin receptor in Hank's solution, the Ca2+ response to erythromycin was almost restricted. Conclusion: It is suggested that motilin could increase [Ca2+]i in myenteric neurons in a dose-dependent manner, and erythromycin may also have this effectivenesss by binding to the motilin receptor.
