甘露醇和AgNO_3对小麦细胞再分化的影响

比较了甘露醇和AgNO3 对小麦 (TriticumaestivumL.)品种保7059幼穗细胞再分化和IAA氧化酶、IAA过氧化物酶活性的影响 .结果表明 ,AgNO3 能够明显提高小麦愈伤组织再生率 ,但甘露醇使其再生率降低 ,AgNO3 对IAA氧化酶和IAA过氧化物酶活性无明显影响,但甘露醇使2种酶活性明显升高 .

草莓离体叶片脱分化与再分化过程中的生理生化变化(简报)

在 1.0mg·L- 1TDZ与 1.0mg·L- 1IAA(培养基A)作用下 ,草莓离体叶组织呼吸速率、蛋白质、核酸和糖类含量以及过氧化氢酶和苯丙氨酸解氨酶活性迅速提高 ,随即很快下降 ,同时形成愈伤组织 ,而后各项指标均维持在低水平 ,愈伤组织不分化 ;在 2 .5mg·L- 1TDZ与 0 .1mg·L- 1IAA(培养基B)作用下 ,上述指标先小幅度提高 ,并维持在同一水平直至愈伤组织形成 ,其后大幅度提高 。

甘露醇对小麦细胞IAA氧化酶、过氧化物酶及GST活性的影响

研究了 1 6g/L甘露醇处理对小麦细胞再分化、细胞IAA氧化酶、IAA过氧化物酶、谷胱甘肽转移酶和过氧化物酶活性的影响。结果表明 ,甘露醇处理使小麦细胞再生能力明显降低 ,引起细胞蛋白质含量、IAA过氧化物酶和GST活性明显降低 ;但使细胞IAA氧化酶和POD活性明显增高。

Differentiation of rat bone marrow stem cells in liver after partial hepatectomy

AIM： To investigate the differentiation of rat bone marrow stem cells in liver after partial hepatectomy. METHODS： Bone marrow cells were collected from the tibia of rat with partial hepatectomy, the medial and left hepatic lobes were excised. The bone marrow stem cells （Thy^＋CD3^-CD45RA^- cells） were enriched from the bone marrow cells by depleting red cells and fluorescence-activated cell sorting. The sorted bone marrow stem cells were labeled by PKH26-GL in vitro and autotransplanted by portal vein injection. After 2 wk, the transplanted bone marrow stem cells in liver were examined by the immunohistochemistry of albumin （hepatocyte-specific marker）. RESULTS： The bone marrow stem cells （Thy^＋CD3^-CD45RA^- cells） accounted for 2.8% of bone marrow cells without red cells. The labeling rate of 10 μM PKH26- GL on sorted bone marrow stem cells was about 95%. There were sporadic PKH26-GL-labeled cells among hepatocytes in liver tissue section, and some of the cells expressed albumin. CONCLUSION： Rat bone marrow stem cells can differentiate into hepatocytes in regenerative environment and may participate in liver regeneration after partial hepatectomy.