肿瘤患者化疗过程中网织红细胞动态变化的观察

目的 了解肿瘤化疗时骨髓受抑和恢复期网织红细胞 (网红 )各参数变化的情况 ,以便确定骨髓造血功能开始恢复的早期指标。方法 用ADVIA 12 0全自动血细胞分析仪对 18例肿瘤患者在化疗过程中的白细胞、血小板、中性粒细胞绝对数 (ANC)和网红计数与分类进行了动态观察。结果 化疗后骨髓造血功能明显受抑 ,白细胞、网红及血小板极度降低 ,其中中性粒细胞绝对数降至 0 .0 2×10 9 L以下、网红绝对数降至 10× 10 9 L以下 ,高荧光强度网红百分率和中荧光强度网红百分率 (HFR % +MFR % )逐步降至最低水平或 0 ,网红分类几乎全部由低荧光强度网红 (LFR)组成。造血功能开始恢复时高荧光强度网红和中荧光强度网红的出现或升高较中性粒细胞绝对数达到 0 .2× 10 9 L早 4 .5d(中位数 )、达到 0 .5× 10 9 L早 7.5d(中位数 ) ,较网红绝对数 (RET # )达到正常范围低限早 15d(中位数 )。结论 HFR +MFR可作为评价肿瘤病人化疗过程中骨髓造血功能开始恢复的较敏感指标。

自体造血干细胞体内迁移术血细胞动态变化与治疗环境

目的 :探讨洁净室外能否安全进行自体造血干细胞体内迁移 (ASCM)术治疗恶性血液病。方法 :对不同治疗环境内患者的主要血细胞动态变化进行全程观察并分析治疗结果。结果 :经不同治疗环境治疗的病人 ,其血细胞恢复期无明显不同 ,治疗相关并发症也无明显差异。结论

纳米氧化锌颗粒对大鼠气管上皮细胞动态变化的影响及毒性机理

纳米氧化锌(Zn O NPs)对呼吸道的毒性损伤作用备受关注,但有关其对呼吸道上皮细胞动态变化的影响机理还有待深入研究.因此,本研究通过将大鼠气管上皮细胞(RTE)暴露于不同浓度(1和10 mg·L^(-1))和不同粒径(50和200 nm)的Zn O NPs中,利用细胞电阻抗检测技术(ECIS)检测细胞动态变化,采用CCK8法检测细胞生长抑制效应,并通过胞内ROS和MDA含量变化探讨其影响机制.ECIS检测结果显示,Zn O NPs暴露下,RTE细胞生长和增殖受到明显抑制,且具有浓度依赖效应.当暴露浓度为1 mg·L^(-1)时,与对照组相比,50 nm暴露组细胞电阻抗值的下调幅度为18%,为200 nm暴露组的1.2倍.Zn O NPs诱导的RTE细胞增殖抑制率具有浓度依赖效应,当暴露浓度为10 mg·L^(-1)时,50和200 nm暴露组细胞增殖抑制率分别为暴露浓度为1 mg·L^(-1)时的2.9和1.4倍.Zn O NPs诱导的RTE细胞氧化应激结果显示,胞内ROS和MDA含量随着纳米颗粒暴露浓度的增加而增加,随着纳米颗粒粒径的减小而增加,具有显著的浓度-和剂量-依赖效应.当Zn O NPs浓度分别为1和10 mg·L^(-1)时,ROS含量分别是对照组的2.8和3.7倍;当暴露浓度为10 mg·L^(-1)时,50 nm暴露组细胞内ROS含量是200 nm暴露组的1.7倍;暴露浓度为1和10 mg·L^(-1)时,50 nm氧化锌处理组诱导的细胞内MDA含量分别是对照组的5.4和7.9倍.Zn O NPs能够影响呼吸道上皮细胞动态变化,从而破环RTE细胞屏障并进入细胞,诱导胞内ROS和MDA水平升高,进而抑制细胞的生长与增殖.研究表明,影响Zn O NPs诱导的RTE细胞动态变化和氧化应激的关键因素是颗粒粒径与暴露浓度.

猪瘟基因疫苗免疫小鼠体液及细胞免疫动态变化研究

目的 :探讨猪瘟基因疫苗免疫小鼠后机体产生体液和细胞免疫发生规律 ,为猪瘟基因疫苗推广应用提供科学依据。方法 :应用FACS、MTT法及间接ELISA试验对猪瘟基因疫苗免疫小鼠脾脏及外周血中CD+4 和CD+8淋巴细胞数、淋巴细胞的转化功能及特异的抗猪瘟病毒血清IgG抗体水平等动态变化进行了观察。结果 :猪瘟基因疫苗免疫小鼠后脾细胞及外周血淋巴细胞对ConA和LPS均有明显的反应性。CD+4 和CD+8细胞数量在免疫后 2 0天开始反应 ,32天达到高峰后开始下降。鼠血清特异性猪瘟病毒血清抗体IgG随免疫时间延长而增加。结论 :猪瘟基因疫苗免疫动物可诱导机体体液及细胞免疫。

细胞间黏附分子-1在实验性自身免疫性脑脊髓炎大鼠中表达的动态变化及作用

目的探讨细胞间黏附分子-1(ICAM-1)在实验性自身免疫性脑脊髓炎(EAE)大鼠中表达的动态变化及其作用。方法分别取免疫后第4、6、8、10、12、14、16、18、20天EAE大鼠脑和脊髓制成石蜡切片,行HE染色和ICAM-1半定量免疫组化分析。结果免疫后第8天ICAM-1表达即出现明显上调,早于临床症状的发生;随免疫后时间的延长,ICAM-1表达呈逐渐增高后缓慢下降的变化趋势,并且与EAE大鼠病情评分呈显著正相关(r=0.57,P=0.003)。结论ICAM-1的表达上调可能在EAE发病中具重要作用。

Dynamic changes and surveillance function of prion protein expression in gastric cancer drug resistance

AIM:To explore the dynamic changes of prion protein (PrPc) in the process of gastric cancer drug resistance and the role of PrPc expression in the prognosis of gastric cancer patients receiving chemotherapy.METHODS:A series of gastric cancer cell lines resistant to different concentrations of adriamycin was established,and the expression of PrPc,Bcl-2 and Bax was detected in these cells.Apoptosis was determined using Annexin V staining.Western blotting and immunohisto-chemistry were performed to detect the expression of PrPc in patients receiving chemotherapy and to explore the role of PrPc expression in predicting the chemosensitivity and the outcome of gastric cancer patients receiving chemotherapy.Follow-up was performed for 2 years.RESULTS:PrPc expression was increased with the increase in drug resistance.Bcl-2,together with PrPc,increased the level of anti-apoptosis of cancer cells.Increased PrPc expression predicted the enhanced level of anti-apoptosis and resistance to anticancer drugs.PrPc expression could be used as a marker for predicting the efficacy of chemotherapy and the prognosis of gastric cancer.Increased PrPc expression predicted both poor chemosensitivity and a low 2-year survival rate.Contrarily,low PrPc expression predicted favorable chemosensitivity and a relatively high 2-year survival rate.CONCLUSION:PrPc expression is associated with histological types and differentiation of gastric cancer cells;The PrPc expression level might be a valuable marker in predicting the efficacy of chemotherapy and the prognosis of gastric cancer patients receiving chemotherapy.

In situ evaluation of cell cultivation on dynamically changed poly(ε-caprolactone) film caused by enzymatic degradation

In situ evaluation of cell cultivation on degrading poly(ε-caprolactone) (PCL) films was studied.New culture surroundings were constructed for cell growth by using PCL films as substrates and adding Pseudomonas cepacia lipase to accelerate biodegradation of PCL films.MTT experiments for 10 h indicated the low cytotoxicity of lipase solution with concentration up to 0.2 mg/mL for MG-63 cells growth on PCL films.With the optimized lipase concentration and degradation time,we studied cell growth behavior on dynamically changed PCL films by adding lipase to the culture surroundings.MTT,fluorescence microscopy and scanning electron microscopy (SEM) were used to evaluate cell viability,proliferation and morphologies.It was found that cell viability and proliferation were not affected by the added lipase solution negatively.In contrast,cells cultured on degrading PCL films showed good growth behavior with clear fusiform shape and pseudopods.Importantly,the enzymatic degradation of PCL films with cells attachment showed distinctive morphology compared to the degradation in lipase solution without cells.The simultaneous cell growth and PCL film degradation were well discussed in this work,which may better understand the interaction between cell growth and polymer degradation.