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虹吸输铁对黄化苹果叶片光合参数及细胞器结构的影响 被引量:1
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作者 高一宁 时晓芳 +1 位作者 侯延杰 薛进军 《广西植物》 CAS CSCD 北大核心 2018年第12期1660-1666,共7页
该研究以红富士苹果品种为材料,通过虹吸输入铁肥的方式,设置输铁液(600倍FeS04·7H20)处理并以输离子水和不输液为对照,观察了处理后苹果树的复绿情况,测定了叶绿素含量、光合参数、进行了叶绿体、线粒体超微结构的电镜扫描。结果... 该研究以红富士苹果品种为材料,通过虹吸输入铁肥的方式,设置输铁液(600倍FeS04·7H20)处理并以输离子水和不输液为对照,观察了处理后苹果树的复绿情况,测定了叶绿素含量、光合参数、进行了叶绿体、线粒体超微结构的电镜扫描。结果表明:输铁后10 d,树体复绿情况明显,复绿等级由2.01变为0.53,复绿的叶片叶绿素含量有显著增加,增幅达到200%。同时,净光合速率提高了68%、蒸腾速率提高了21%、气孔导度提高了49%,胞间CO2浓度降低了100%。输铁处理后的单个细胞叶绿体个数比对照有显著增加,且个体更饱满,淀粉粒和嗜锇颗粒有明显减少;基粒片层结构规律整齐,基质清晰,被膜结构完整,线粒体内嵴清晰度高,数量增多。在输铁处理后,苹果树体复绿明显,叶绿素合成能力恢复,叶绿体、线粒体被膜结构均得到修复,表明用虹吸的方法将铁肥输入树体对苹果缺铁黄化病有很好的疗效。 展开更多
关键词 铁肥 虹吸输液 黄化 苹果 光合参数 细胞器结构
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动脉瘤性蛛网膜下腔出血后亚细胞器结构及功能的研究进展
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作者 吴培 李俞辰 +7 位作者 戴家兴 徐善才 郑秉杰 季志勇 王春雷 张广 亓敬涛 史怀璋 《中国脑血管病杂志》 CAS CSCD 北大核心 2015年第8期439-442,共4页
动脉瘤性蛛网下腔出血(aneurysm subarachnoid hemorrhage,aSAH)预后差主要与早期脑损害(early brain injury,EBI)和随后出现的脑血管痉挛(cerebral vasospasm,cvs)密切相关。最新研究显示,
关键词 颅内动脉瘤 蛛网膜下腔出血 综述 细胞器结构及功能
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模型在《细胞器的结构和功能》教学中的应用
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作者 孙晶晶 《中学教学参考》 2018年第26期96-96,共1页
教学《细胞器的结构和功能》一节时,因细胞器的结构微观抽象,常见的橡皮泥模型较难在黑板上展示,教学效果不佳。而利用3D打印画笔制作相对精致的细胞器模型,可借助模型引导学生小组合作探究细胞器的结构和功能,促进学生深刻理解“结构... 教学《细胞器的结构和功能》一节时,因细胞器的结构微观抽象,常见的橡皮泥模型较难在黑板上展示,教学效果不佳。而利用3D打印画笔制作相对精致的细胞器模型,可借助模型引导学生小组合作探究细胞器的结构和功能,促进学生深刻理解“结构与功能相适应”的观点。 展开更多
关键词 3D打印画笔 模型 细胞器结构和功能
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旱柳枝条皮层叶绿体的光化学特性及结构的特化 被引量:7
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作者 刘俊祥 于永畅 +3 位作者 郎蓬蓬 沈豪 巨关升 孙振元 《林业科学》 EI CAS CSCD 北大核心 2018年第5期30-35,共6页
【目的】皮层光合对CO2的再固定可增加植株的碳收益,在叶片光合受阻时保障植株的生存延续。光合作用决定于叶绿体的结构和功能,研究皮层叶绿体的光化学特性及其结构的特化,可为揭示皮层光合运行、调节的生理和分子机制奠定基础。【方法... 【目的】皮层光合对CO2的再固定可增加植株的碳收益,在叶片光合受阻时保障植株的生存延续。光合作用决定于叶绿体的结构和功能,研究皮层叶绿体的光化学特性及其结构的特化,可为揭示皮层光合运行、调节的生理和分子机制奠定基础。【方法】以旱柳当年生枝条为材料,在明确枝条中叶绿体的分布及其光化学效率的基础上,解析枝条皮层叶绿体与叶片叶绿体的光化学特性和结构的差异。【结果】旱柳枝条从髓到表皮均有叶绿体分布,皮层组织叶绿体的最大光化学效率最高,显著高于维管组织和髓。枝条皮层叶绿体的叶绿素b与叶绿素a、类胡萝卜素与总叶绿素的比例显著高于叶片叶绿体。枝条皮层叶绿体的PSⅡ激发能分配比例是叶片叶绿体的1.4倍。皮层叶绿体较叶片叶绿体具有较高的长、短轴比例。皮层叶绿体具有完整的亚细胞器结构,其中基粒片层占总片层数的比例、淀粉粒占叶绿体的比例显著高于叶片叶绿体。【结论】皮层组织叶绿体是枝条光合的主要贡献者。皮层叶绿体通过增加受光面积、提高天线色素比例,增加对枝条中有限光能的捕捉;通过提高光保护色素的比例减轻光氧化胁迫;通过增加类囊体的垛叠面积,促进光能向反应中心PSⅡ的传递;通过储藏淀粉,为枝条的非结构性碳水化合物需求提供保障。皮层叶绿体通过对光合色素组成的调整和亚细胞器结构的特化来适应枝条内的理化环境,最大限度地利用枝条中有限的光能。 展开更多
关键词 枝条皮层组织 叶绿体 光化学特性 细胞器结构特化
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刀额新对虾脑部胶质细胞和神经鞘
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作者 姚泊 《广州师院学报(自然科学版)》 1994年第2期73-77,共5页
本文用透射电镜观察了刀额新对虾(Metapenaeus ensis)脑部神经胶质细胞和神经纤维髓鞘;结果表明。这些胶质细胞呈圆形或椭圆形,直径约6400nm。在胞质中,没有观察到有线粒体,高尔基体等细胞结构,细胞核大... 本文用透射电镜观察了刀额新对虾(Metapenaeus ensis)脑部神经胶质细胞和神经纤维髓鞘;结果表明。这些胶质细胞呈圆形或椭圆形,直径约6400nm。在胞质中,没有观察到有线粒体,高尔基体等细胞结构,细胞核大电子致密的异染色质位于核边缘且连成块状,髓鞘直径约6400—9300nm。鞘层厚约500—670nm。在髓鞘层中存在着微管结构,微管直径约83nm。 展开更多
关键词 刀额新对虾 神鞘 胶质细胞 甲壳动物 细胞器结构 微管结构
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活细胞中亚细胞器的超分辨结构特征 被引量:2
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作者 王冠晨 陈同生 《中国激光》 EI CAS CSCD 北大核心 2022年第20期86-94,共9页
亚细胞器是细胞的重要组成单位,其形态结构与动力学特性直接反映了细胞的生理状态。21世纪初新兴的结构光照明显微技术、受激发射损耗显微技术和单分子定位成像技术等超分辨显微成像技术,巧妙地绕过了光学衍射极限对成像分辨率的限制,... 亚细胞器是细胞的重要组成单位,其形态结构与动力学特性直接反映了细胞的生理状态。21世纪初新兴的结构光照明显微技术、受激发射损耗显微技术和单分子定位成像技术等超分辨显微成像技术,巧妙地绕过了光学衍射极限对成像分辨率的限制,目前已被广泛应用于活细胞亚细胞器精细结构的观察及其动力学过程的监测上。本文首先介绍了上述三种超分辨显微成像技术的基本原理和特点,然后介绍了活细胞中细胞核、细胞骨架、线粒体、内质网等亚细胞器的超分辨精细结构和动力学特性,最后讨论了亚细胞器超分辨精细结构成像与机器学习、图像处理相结合的发展潜力。 展开更多
关键词 生物光学 超分辨显微术 荧光显微镜 细胞器精细结构 机器学习
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Influence of Copper Stress on Ultra-structure of Wheat Seedlings 被引量:6
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作者 于红丽 姚瑞芹 肖昕 《Agricultural Science & Technology》 CAS 2010年第5期7-9,45,共4页
Transmission Electron Microscope (TEM) Technology was used to investigate the effect of 25,100 and 200 mg/kg copper on ultra-structure of root tip and leaf blade of wheat. Result showed that serious damage was found w... Transmission Electron Microscope (TEM) Technology was used to investigate the effect of 25,100 and 200 mg/kg copper on ultra-structure of root tip and leaf blade of wheat. Result showed that serious damage was found with Copper of 25,100 and 200 mg/kg. Plasmolysis,concentrated cytoplasm,chloroplast inflation,lamellar structure disturbance,capsule disappearance and disintegration,mitochondria structures ambiguity and vacuolization were all symptoms under Cu stress. There were positive correlation between concentration of coper stress and the degree of injury,and the degree of injury of copper were different in different organelles. Mitochondria were the most sensitive organelles,and there was patient difference in the same organelles of different parts. 展开更多
关键词 Wheat Copper Cell ultra-structure
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Application of photobioreactors to cultivation of microalgae
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作者 陈雷 王光玉 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2003年第2期221-224,共4页
An overview of photobioreactors now in use for production of microalgae world wide is presented, and the application of photobioreactors to the cultivation of microalgae is discussed in detail. It is pointed out that ... An overview of photobioreactors now in use for production of microalgae world wide is presented, and the application of photobioreactors to the cultivation of microalgae is discussed in detail. It is pointed out that high cell density and industrial production of microalgae can be achieved using many kinds of closed photobioreactors including fermentor, tubular and flat plate photobioreactors, and the cultivation of Spirulina, Chlorella, Dunaliella tertiolecta and Porphyridium cruentrim by photobioreactors can achieve higher and steadier productivity than the cultivation of microalgae by an open air system. More and more researches indicate that tubular and flat plate photobioreactors are the development trend for photobioreactors with bubbles and air lift stirrers, and high bright light emitting diodes are the most economic light source with great potential for future development of photobioreactors. Photobioreactors can also be used for the production of high value metabolite (EPA or DHA) using some microalgae species for energy development and environment protection. 展开更多
关键词 MICROALGAE PHOTOBIOREACTOR CULTURE
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植物应答低温胁迫机制的研究进展 被引量:34
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作者 计淑霞 戴绍军 刘炜 《生命科学》 CSCD 北大核心 2010年第10期1013-1019,共7页
低温是植物生长过程中遇到的主要环境胁迫因子之一,而植物响应低温胁迫是一个多因素协同作用的过程,涉及到复杂的基因表达调控网络。尤其是低温下植物体内生理生化、细胞骨架结构及基因表达调控等方面的改变及相关机制,一直受到研究者... 低温是植物生长过程中遇到的主要环境胁迫因子之一,而植物响应低温胁迫是一个多因素协同作用的过程,涉及到复杂的基因表达调控网络。尤其是低温下植物体内生理生化、细胞骨架结构及基因表达调控等方面的改变及相关机制,一直受到研究者的普遍关注。该文主要从细胞学及分子生物学等角度入手,将低温胁迫下植物对低温的响应及可能机制进行综述,着重对植物通过细胞内部细胞器结构与功能的改变来抵御或适应低温,尤其对细胞骨架,以及低温信号转导受体及中间体、下游胁迫相关基因的表达及其在细胞内部的调控及应答机制等方面的作用进行探讨,为耐低温植物新品种的培育及农业生产实践提供理论指导。 展开更多
关键词 低温 植物 细胞器结构与功能 信号转导受体及中间体 胁迫相关基因 耐低温植物新品种
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New observations on the effect of camellia oil on fatty liver disease in rats 被引量:4
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作者 Chun-xue LI Li-rong SHEN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2020年第8期657-667,共11页
Camellia oil has become an important plant oil in China in recent years,but its effects on non-alcoholic fatty liver disease(NAFLD)have not been documented.In this study,the effects of camellia oil,soybean oil,and oli... Camellia oil has become an important plant oil in China in recent years,but its effects on non-alcoholic fatty liver disease(NAFLD)have not been documented.In this study,the effects of camellia oil,soybean oil,and olive oil on NAFLD were evaluated by analyzing the fatty acid profiles of the plant oils,the serum lipids and lipoproteins of rats fed different oils,and by cytological and ultrastructural observation of the rats’hepatocytes.Analysis of fatty acid profiles showed that the polyunsaturated fatty acid(PUFA)n-6/n-3 ratio was 33.33 in camellia oil,12.50 in olive oil,and 7.69 in soybean oil.Analyses of serum lipids and lipoproteins of rats showed that the levels of total cholesterol and low-density lipoprotein cholesterol in a camellia oil-fed group(COFG)were lower than those in an olive oil-fed group(OOFG)and higher than those in a soybean oil-fed group(SOFG).However,only the difference in total cholesterol between the COFG and SOFG was statistically significant.Cytological observation showed that the degree of lipid droplet(LD)accumulation in the hepatocytes in the COFG was lower than that in the OOFG,but higher than that in the SOFG.Ultrastructural analysis revealed that the size and number of the LDs in the hepatocytes of rats fed each of the three types of oil were related to the degree of damage to organelles,including the positions of nuclei and the integrity of mitochondria and endoplasmic reticulum.The results revealed that the effect of camellia oil on NAFLD in rats was greater than that of soybean oil,but less than that of olive oil.Although the overall trend was that among the three oil diets,those with a lower n-6/n-3 ratio were associated with a lower risk of NAFLD,and the effect of camellia oil on NAFLD was not entirely related to the n-6/n-3 ratio and may have involved other factors.This provides new insights into the effect of oil diets on NAFLD. 展开更多
关键词 Camellia oil Fatty acid Lipid droplet Hepatocyte ultrastructure ORGANELLE
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Cellular structure of detonation utilized in propulsion system 被引量:2
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作者 ZHANG XuDong FAN BaoChun +1 位作者 GUI MingYue PAN ZhenHua 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2012年第10期1915-1924,共10页
How to confine a detonation in a combustor is a key issue of detonation applications in propulsion systems.Based on achieving schemes,detonations applied in the combustor,including pulse detonation wave(PDW),oblique d... How to confine a detonation in a combustor is a key issue of detonation applications in propulsion systems.Based on achieving schemes,detonations applied in the combustor,including pulse detonation wave(PDW),oblique detonation wave(ODW) and rotating detonation wave(RDW),are different from that described by the classic CJ theory in fine structures and its self-sustaining mechanisms.In this work,the cellular structures and flow fields of ODW and RDW were obtained numerically,and the fundamental characteristics and self-sustaining mechanisms of the detonations were analyzed and discussed.ODW front consists of three parts:the ZND-like front,the single-headed triple point front and the dual-headed triple point front.Cellular structures of RDW are heterogeneous,and the cell size near the outer wall is smaller than that near the inner wall. 展开更多
关键词 propulsion system fundamental characteristics self-sustaining mechanisms classic CJ theory
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