酰胺质子转移成像在预测脑胶质瘤Ki67细胞增殖水平中的应用价值

目的 探讨酰胺质子转移成像(APTw)与脑胶质瘤Ki67细胞增殖指数的相关性,并分析其预测的准确度。方法 收集来自北京天坛医院的63例经手术病理证实且于术前2周内进行APTw扫描的脑胶质瘤患者,最终共纳入59例脑胶质瘤患者。按照免疫组化中获取的Ki67细胞增殖指数,将患者分为低增殖组(细胞增殖指数<20%,n=39)和高增殖组(细胞增殖指数≥20%,n=20),并测量两组肿瘤APTw信号强度。采用Pearson检验分析APTw与Ki67细胞增殖指数之间的相关性;采用Mann-Whitney U检验分析APTw信号强度在两增殖组间的差异;以APTw信号强度为影像标志物建立预测模型来预测脑胶质瘤Ki67细胞增殖水平,绘制ROC曲线评估其诊断效能,最后计算约登指数获取APTw信号强度的临界值。结果 APTw信号强度与Ki67细胞增殖指数之间呈正相关(r=0.629,P<0.001);APTw信号强度在两个Ki67增殖水平组间差异有统计学意义(Z=4.539,P<0.001);该预测模型的曲线下面积为0.863(95%CI:0.770~0.956),敏感度为1,特异性为0.718;约登指数结果显示该预测模型中APTw信号强度的临界值为2.55。结论 APTw信号强度可以作为影像标志物来预测脑胶质瘤Ki67细胞增殖水平,并且具有良好的诊断效能及敏感性;当APTw信号强度大于2.55时,脑胶质瘤Ki67细胞增殖水平更倾向于高增殖水平,反之,更倾向于低增殖水平。

钆塞酸二钠增强MRI列线图可在患者术前有效预测肝细胞癌Ki-67的表达

目的探讨钆塞酸二钠增强MRI列线图在术前预测肝细胞癌Ki-67表达状态的应用价值。方法回顾性分析本院2022年1月~2024年5月经病理诊断的122例肝细胞癌患者的资料,根据术后免疫组化细胞增殖水平(Ki-67指数)将患者分为Ki-67高表达组(n=71)和Ki-67低表达组(n=51),并按7:3比例随机分为训练集(n=85)和验证集(n=37),分析两组患者影像学参数[肿瘤直径、边缘、假包膜、瘤体区表观弥散系数(ADC)、肝胆期信号强度与正常肝实质信号强度比(SIR)、瘤周低信号]、AFP浓度及肿瘤分化程度差异,纳入LASSO回归模型筛选出最具价值的参数,并行多因素Logistic回归分析建立预测模型构建列线图,通过ROC曲线、校准曲线和决策曲线评估模型效能。结果训练集及验证集Ki-67高表达组甲胎蛋白≥20 ng/mL、肿瘤边缘不光滑、瘤周低信号高于Ki-67低表达组,ADC值和SIR低于Ki-67低表达组,差异有统计学意义(P<0.05),而肿瘤直径、假包膜的差异无统计学意义(P>0.05)。应用LASSO回归筛选出3个最有价值参数(肿瘤ADC值、SIR及肿瘤分化程度),多因素Logistic回归分析显示ADC值及SIR是Ki-67表达影响因素,基于以上参数建立列线图模型,其校准曲线与理想曲线贴合良好,其ROC曲线下面积为0.827,敏感度为81.2%,特异度为80%。结论钆塞酸二钠增强MRI列线图模型可在术前有效预测肝细胞癌Ki-67表达状态,从而实现肝细胞癌患者风险分层及个体化治疗。

The experimental research on the inhibiting impacts of RNAi on Cyclin E in breast cancer cell line

Objective:The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.Methods:Human breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2.SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level,respectively.The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay,while cell cycle was measured by flow cytometry(FCM).Results:The plasmid could reduce the expression of Cyclin E.The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression.The transfected MCF-7 was arrested at G1 phase of cell cycle.Conclusion:The inhibition of Cyclin E can decrease the breast cancer cell's growth,increase its sensation to chemical therapy and slow down its cell cycle.The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.

Pin1 expression affects cell proliferation and apoptosis of SW620 cells in colorectal carcinoma

Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods:We constructed a plasmid of RNA interfering(shRNA) for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western blotting.Results:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06 ± 0.04 for the P-shRNA/SW620 cells,and 0.32 ± 0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ± 1.55% for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13 ± 0.04 for the P-shRNA/SW620 cells,and 0.36 ± 0.08 for the P-Con/SW620 cells.Conclusion:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.