肿瘤相关基因N-myc、Fas、MTA1、nm23-H1对子宫内膜癌细胞增殖转移的影响

目的探讨原癌基因N-myc、抑癌基因Fas、转移促进基因MTA1及转移抑制基因nm23-H1对子宫内膜癌细胞增殖转移的影响。方法采用实时荧光定量RT-PCR检测正常子宫内膜组织、子宫内膜腺癌未转移患者及转移患者标本各30例中N-myc、Fas、MTA1、nm23-H1的基因表达情况,并行比较分析。结果 (1)子宫内膜癌组织中N-myc、MTA1基因扩增水平均高于正常子宫内膜组织,差异有统计学意义(均P<0.05);(2)子宫内膜癌组织中Fas、nm23-H1基因扩增水平均低于正常子宫内膜组织,差异有统计学意义(均P<0.05);(3)转移子宫内膜癌组织中MTA1基因扩增水平均高于未转移组,nm23-H1基因扩增水平均低于未转移组,差异均有统计学意义(均P<0.05);(4)子宫内膜癌组织转移组与未转移组中N-myc、Fas基因扩增水平均具有差异性,差异有统计学意义(均P<0.05)。结论 N-myc、MTA1基因能促进子宫内膜癌的细胞增殖,Fas、nm23-H1基因能抑制子宫内膜癌的细胞增殖;MTA1基因可促进子宫内膜癌的细胞转移,nm23-H1基因可抑制子宫内膜癌的细胞转移。

实时荧光定量RT-PCR技术检测肿瘤相关基因对子宫颈癌细胞增殖转移的影响

肿瘤的恶变是一系列过程,其中癌基因激活、抑制癌基因失活是相应的分子机制。癌症的发生和发展是癌基因与抑癌基因相互作用的结果,恶性肿瘤导致患者死亡主要是因为癌细胞的侵袭和转移[1]。大量试验数据表明癌症的发生是因为一些控制细胞增殖转移基因的表达失调和细胞周期的调控失调。腹腔镜手术中电能及超声刀等手术器械的使用及人工气腹状态的建立,使机体处于应激状态,因为机体环境的改变产生和释放各种细胞因子,引起细胞酶活性的改变,细胞内相关基因的诱导和抑制平衡的移动,细胞内离子水平的变化,使细胞的功能状态和凋亡发生改变[2]。本研究对不同手术患者术后的子宫颈标本进行检测,在基因水平上探讨子宫颈癌细胞增殖转移的本质,同时从分子生物学基因表达方面对宫颈癌手术方式的选择提供一定参考。

异氟醚下调MYC表达抑制高级别脑胶质瘤细胞增殖转移

目的探究异氟醚对高级别脑胶质瘤细胞MYC基因表达及细胞增殖转移能力的影响。方法分别采用含2%异氟醚的气体环境及常规气体环境(5%CO2)培养对数生长期的人脑胶质瘤细胞系SHG-44 6 h,作为异氟醚组及对照组;CCK-8实验检测两组细胞气体暴露完成后0、24、48、72 h相对增殖能力;Transwell侵袭及迁移实验检测两组细胞侵袭及迁移能力;Western Blotting检测两组细胞C-Myc及N-Myc的蛋白表达。结果异氟醚组细胞于暴露完成后48、72 h时增殖能力较对照组细胞显著下调,差异具有统计学意义(P<0.01);异氟醚组穿透基质胶的侵袭细胞数、穿过微孔的迁移细胞数均较对照组显著减少,差异均具有统计学意义(P<0.01、0.001);异氟醚组C-Myc及N-Myc的表达较对照组均显著下调,差异具有统计学意义(P<0.01)。结论异氟醚可通过下调C-Myc及N-Myc基因的表达,抑制人高级别脑胶质瘤细胞的增殖转移。

ADRM1对胰腺癌细胞增殖和转移的影响及相关机制研究

目的探讨黏附调节分子1(ADRM1)对胰腺癌细胞增殖和转移的影响及其相关机制。方法GEPIA数据库分析胰腺癌中的ADRM1 mRNA表达水平及其对患者预后的影响。EdU、CCK-8、Transwell实验分析抑制或过表达ADRM1对胰腺癌细胞增殖和转移的影响。采用生物信息学分析及Western blot探讨ADRM1调控胰腺癌细胞增殖和转移的相关机制。结果ADRM1 mRNA在胰腺癌中高表达,其表达水平与患者总生存率无关,与患者无病生存率有关,ADRM1 mRNA高水平组无病生存率低于ADRM1 mRNA低水平组。抑制ADRM1可以减弱胰腺癌细胞增殖和转移能力,过表达ADRM1可以增强胰腺癌细胞增殖和转移能力。ADRM1可以激活JAK酪氨酸蛋白激酶2-信号传导和转录激活因子3(JAK2-STAT3)信号通路。结论ADRM1作为促癌因子,在胰腺癌中高表达并促进胰腺癌细胞增殖和转移,其机制可能与激活JAK2-STAT3信号通路有关。

HGF/SF-Met signaling in tumor progression

Tumor progression is a multi-step process that requires a sequential selection of specific malignant phenotypes. Met activation may induce different phenotypes depending on tumor stage: inducing proliferation and angiogenesis in pri- mary tumors, stimulating motility to form micrometastases, and regaining the proliferation phenotype to form overt metastases. To study how HGF/SF-induced proliferative phenotypes switch to the invasive phenotype is important for understanding the mechanism of tumor progression and will provide an attractive target for cancer intervention and therapy.

Decreasing activity of NF-κB inhibits the proliferation of A549 cells via the down-regulation of cyclin D1 expression

Objective: To investigate the effect of decreasing activity of NF-κB on proliferation of A549 cell line and the pos-sible molecular mechanism. Methods: The recombinant plasmid PCDNA3.1(+)/IκBα expressing IκBα was constructed. The recombinant plasmid was then transfected to A549 cell. The activity of NF-κB, cell proliferation, and cyclin D1 expression were observed. Results: Our results showed that transfecting PCDNA3.1(+)/IκBα inhibited activity of NF-κB in A549 cells, and decreasing activity of NF-κB inhibited proliferation of A549 cells. Decreasing activity of NF-κB was accompanied with down-regulation of cyclin D1 expression. Conclusion: Decreasing activity of NF-κB inhibited proliferation of A549 cells, and the molecular mechanism of the inhibition effect may be down-regulation of cyclin D1 expression.

Local injection of liposomal adriamycin to inhibit metastatic cell proliferation in axillary nodes in rabbits bearing breast tumors

Objective: To assess the inhibitory effects of local injection of liposomal adriamycin (LADR) on the proliferation of lymph node metastases in rabbits bearing VX2 carcinoma in the mammary gland. Methods:Thirty female New Zealand white rabbits were divided into 3 groups, with 10 in each. VX2 tumor mass suspensions were injected into the breast tissues of rabbits. Treatment initiated once the axillary lymph node reached 5 mm in the maximum diameter. Group 1 received a sham treatment. Group 2 received a subcutaneous injection of LADR adjacent to tumor. Group 3 received an intravenous injection of free ADR (FADR) at the same dose and concentration to group 2. The breast tumors and axillary lymph nodes were resected after the treatment was repeated 3 times. The tumor and node sizes before and after treatment were measured. PCNA mRNA expressions in breast tumors and axillary nodes were determined using RT-PCR. Results: The mean growth ratios of lymph nodes after treatment were 3. 70, 1. 55, and 2. 89,respectively, in groups 1,2, and 3. The slowest node growth was observed in animals of group 2, with significant differences from group 1 (P<0. 001) and group 3 (P = 0. 002). The relative values of PCNA mRNA expression in lymph nodes were 0. 541, 0. 329,and 0. 450, respectively, in groups 1,2, and 3. Group 2 exhibited a significantly reduced PCNA mRNA expression in metastatic lymph node, as compared to group 1 (P<0. 001) and group 3 (P = 0. 004). Intravenous FADR injection effectively lowered the mRNA expressions of PCNA in breast tumors, which were not apparently altered after local LADR injection. Conclusion: Local injection of LADR holds a strong inhibitory effect on the proliferation of metastatic tumor cells in lymph nodes and appears to be an effective method for the treatment of lymphatic metastases of breast cancer.

Curcumin-encapsulated polymeric nanoparticles for metastatic osteosarcoma cells treatment

Osteosarcoma is a high-class malignant bone cancer with a less than 20% five-year survival rate due to its early metastasis potential. There is an urgent need to develop a versatile and innoxious drug to treat metastatic osteosarcoma.Curcumin（Cur） has shown its potential for the treatment of many cancers; however,the clinical implication of native curcumin is severely hindered by its intrinsic property. In this study,a mixed system of monomethoxy（polyethylene glycol）-poly（d,l-lactide-co-glycolide）/poly（ε-caprolactone）（m PEGPLGA/PCL） was used to build a formulation of curcuminencapsulated nanoparticles（Cur-NPs）,which significantly improved the solubility,stability and cellular uptake of curcumin. Moreover,the Cur-NPs were superior to free curcumin in the matter of inhibition on the proliferation,migration and invasion of osteosarcoma 143B cells. It was found that both free curcumin and Cur-NPs could decrease the expressions of c-Myc and MMP7 in the level of mRNA and protein,which explained why free curcumin and Cur-NPs could inhibit the proliferation and invasion of metastatic osteosarcoma 143B cells. The Cur-NPs provided a promising strategy for metastatic osteosarcoma treatment.