Automatic reading procedures in colon cells biopsies allow a faster and precise reading of microscopic biopsies. These procedures implement automatic image segmentation in order to classify cell types as cancerous or ...Automatic reading procedures in colon cells biopsies allow a faster and precise reading of microscopic biopsies. These procedures implement automatic image segmentation in order to classify cell types as cancerous or noncancerous. The authors have developed a new approach aiming to detect colon cancer cells derived from the ＂Snake＂ method but using a progressive division of the dimensions of the image to achieve rapid segmentation. The aim of the present paper was to classify different cancerous cell types based on nine morphological parameters and on probabilistic neural network. Three types of cells were used to assess the efficiency of our classifications models, including BH （Benign Hyperplasia）, IN （Intraepithelial Neoplasia） that is a precursor state for cancer, and Ca （Carcinoma） that corresponds to abnormal tissue proliferation （cancer）. Results showed that among the nine parameters used to classify cells, only three morphologic parameters （area, Xor convex and solidity） were found to be effective in distinguishing the three types of cells. In addition, classification of unknown cells was possible using this method.展开更多
The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not ...The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of ~2 m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development.展开更多
文摘Automatic reading procedures in colon cells biopsies allow a faster and precise reading of microscopic biopsies. These procedures implement automatic image segmentation in order to classify cell types as cancerous or noncancerous. The authors have developed a new approach aiming to detect colon cancer cells derived from the ＂Snake＂ method but using a progressive division of the dimensions of the image to achieve rapid segmentation. The aim of the present paper was to classify different cancerous cell types based on nine morphological parameters and on probabilistic neural network. Three types of cells were used to assess the efficiency of our classifications models, including BH （Benign Hyperplasia）, IN （Intraepithelial Neoplasia） that is a precursor state for cancer, and Ca （Carcinoma） that corresponds to abnormal tissue proliferation （cancer）. Results showed that among the nine parameters used to classify cells, only three morphologic parameters （area, Xor convex and solidity） were found to be effective in distinguishing the three types of cells. In addition, classification of unknown cells was possible using this method.
基金supported by the National Basic Research Program of China (2011CB809101)the National Natural Science Foundation of China (30730013)
文摘The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of ~2 m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development.
