细胞机械特性作为一种无标签(Label-free)的生物标记,正得到越来越多的关注.然而现有进行细胞机械特性测量的方法多以手工模式进行,耗时长、效率低下,无法满足生物学统计分析对大批量样品测试的要求.针对该问题,本文在原子力显微镜(Atom...细胞机械特性作为一种无标签(Label-free)的生物标记,正得到越来越多的关注.然而现有进行细胞机械特性测量的方法多以手工模式进行,耗时长、效率低下,无法满足生物学统计分析对大批量样品测试的要求.针对该问题,本文在原子力显微镜(Atomic force microscopy,AFM)基础上,建立了一套高速自动化的细胞机械特性测量系统.该系统利用图像处理方法来识别细胞,利用局部扫描来实现AFM针尖和细胞相对位置的精确标定,进而不需要AFM成像就能实现细胞机械特性的连续测定,配合上程序化控制的运动载物平台,可以高速自动化完成大范围区域内细胞机械特性的批量规模化测量.实验结果表明,该系统可以使得细胞机械特性的测量效率提高27倍,从而为Label-free生物标记的批量化测试提供了技术支撑.展开更多
In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both no...In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both normal and C_(60) infiltrated red blood cells (RBC) to gain data of mechanical characteristics of the membrane.Our results show that the mechanical properties of human RBC membrane seem to be altered due to the presence of C_(60) NPs.The resistance and ultimate strength of the C_(60) infiltrated RBC membrane significantly decrease.We also explain the mechanism of how C_(60) NPs infiltration changes the mechanical properties of the cell membrane by predicting the structural change of the lipid bilayer caused by the C_(60) infiltration at molecular level and analyze the interactions among molecules in the lipid bilayer.The potential hazards and application of the change in mechanical characteristics of the RBCs membrane are also discussed.Nanotoxicity of C_(60) NPs may be significant for some biological cells.展开更多
文摘细胞机械特性作为一种无标签(Label-free)的生物标记,正得到越来越多的关注.然而现有进行细胞机械特性测量的方法多以手工模式进行,耗时长、效率低下,无法满足生物学统计分析对大批量样品测试的要求.针对该问题,本文在原子力显微镜(Atomic force microscopy,AFM)基础上,建立了一套高速自动化的细胞机械特性测量系统.该系统利用图像处理方法来识别细胞,利用局部扫描来实现AFM针尖和细胞相对位置的精确标定,进而不需要AFM成像就能实现细胞机械特性的连续测定,配合上程序化控制的运动载物平台,可以高速自动化完成大范围区域内细胞机械特性的批量规模化测量.实验结果表明,该系统可以使得细胞机械特性的测量效率提高27倍,从而为Label-free生物标记的批量化测试提供了技术支撑.
基金supported by the National Natural Science Foundation of China (Grant Nos.10902128,11072271,10972239,51172291)Fundamental Research Funds for the Central Universities,New Century Excellent Talents in University and Research Funds for the Doctoral Program of Higher Education
文摘In this paper,we introduce our finding of the effects of C_(60) nanoparticles (NP) infiltration on mechanical properties of cell and its membrane.Atomic force microscopy (AFM) is used to perform indentation on both normal and C_(60) infiltrated red blood cells (RBC) to gain data of mechanical characteristics of the membrane.Our results show that the mechanical properties of human RBC membrane seem to be altered due to the presence of C_(60) NPs.The resistance and ultimate strength of the C_(60) infiltrated RBC membrane significantly decrease.We also explain the mechanism of how C_(60) NPs infiltration changes the mechanical properties of the cell membrane by predicting the structural change of the lipid bilayer caused by the C_(60) infiltration at molecular level and analyze the interactions among molecules in the lipid bilayer.The potential hazards and application of the change in mechanical characteristics of the RBCs membrane are also discussed.Nanotoxicity of C_(60) NPs may be significant for some biological cells.
