The cellular neural/nonlinear network （CNN） is a powerful tool for image and video signal processing, robotic and biological visions. This paper introduces a kind of CNNs with performance of extracting closed domain...The cellular neural/nonlinear network （CNN） is a powerful tool for image and video signal processing, robotic and biological visions. This paper introduces a kind of CNNs with performance of extracting closed domains in binary images, and gives a general method for designing templates of such a kind of CNNs. One theorem provides parameter inequalities for determining parameter intervals for implementing prescribed image processing functions, respectively. Examples for extracting closed domains in binary scale images are given.展开更多
OBJECTIVE: To clarify growth inhibition in pancreatic cancer cells by interference with the hTR component of the telomerase reverse transcriptase enzymatic complex. METHODS: A 593 bp full length hTR cDNA was subcloned...OBJECTIVE: To clarify growth inhibition in pancreatic cancer cells by interference with the hTR component of the telomerase reverse transcriptase enzymatic complex. METHODS: A 593 bp full length hTR cDNA was subcloned into a mammalian expression vector pcDNA3.1(-) in the antisense orientation to construct an antisense hTR expression plasmid. These were introduced into panc1 cells, a human pancreatic carcinoma cell line, by lipofectin and G418-resistant stable transformants were expanded. Resulting stable clones were screened for the presence of the hTR insert by PCR with T7 and BGH reverse primers located on the flanks of the multiclonal site of the pcDNA3.1 vector. Cell growth rate, hTR expression, telomerase activity and anchorage-independent growth properties were analyzed. RESULTS: Significant downregulation of endogenous hTR was evident in the antisense-hTR transformed cells and telomerase activity was markedly decreased compared to control cells in standard TRAP assays. Furthermore, cell proliferation and the anchorage-independent growth ability in antisense-hTR expressing cells were significantly decreased compared with control parental cells. However, no crisis or senescence phenomena were observed. CONCLUSIONS: These data indicate that hTR may be a critical component of human telomerase activity and suggest that downregulation of the RNA component of human telomerase is a possible target for anticancer strategies.展开更多
The silver （Ag） nanowire arrays with regular and uniform size were successfully fabricated inside the nanochannels of anodic aluminum oxide （AAO） template by a simple paired cell method. X-ray diffi＇action （XRD...The silver （Ag） nanowire arrays with regular and uniform size were successfully fabricated inside the nanochannels of anodic aluminum oxide （AAO） template by a simple paired cell method. X-ray diffi＇action （XRD） and scanning elec- tron microscopy （SEM） results indicate that the as-synthesized samples are composed of face-centered cubic structure, and the average diameter is about 60-70 nm. Transmission electron microscopy （TEM） and the corresponding fast Fourier transformation （FFT） results show that Ag nanowires have a preferred single-crystal structure. Ultravio- let-visible （UV-vis） spectrum of Ag nanowire arrays exhibits UV emission band at 383 nm which can be attributed to the transverse dipole resonance ofAg nanowire arrays. A good surface-enhanced Raman scattering （SERS） spectrum is observed by excitation with a 514.5 nm laser, and the intensity of the SERS peak is about 23 times higher than that of the normal Raman peak measured from an empty AAO template. The high enhancement factor suggests that this method can be used to fabricate SERS sensor with high efficiency.展开更多
基金The project supported by National Natural Science Foundation of China under Grant No. 70271068, the Foundation for University Key Teachers, and the Research Fund for the Doctoral Program of Higher Education of the Ministry of Education of China under Grant No. 200200080004
文摘The cellular neural/nonlinear network （CNN） is a powerful tool for image and video signal processing, robotic and biological visions. This paper introduces a kind of CNNs with performance of extracting closed domains in binary images, and gives a general method for designing templates of such a kind of CNNs. One theorem provides parameter inequalities for determining parameter intervals for implementing prescribed image processing functions, respectively. Examples for extracting closed domains in binary scale images are given.
文摘OBJECTIVE: To clarify growth inhibition in pancreatic cancer cells by interference with the hTR component of the telomerase reverse transcriptase enzymatic complex. METHODS: A 593 bp full length hTR cDNA was subcloned into a mammalian expression vector pcDNA3.1(-) in the antisense orientation to construct an antisense hTR expression plasmid. These were introduced into panc1 cells, a human pancreatic carcinoma cell line, by lipofectin and G418-resistant stable transformants were expanded. Resulting stable clones were screened for the presence of the hTR insert by PCR with T7 and BGH reverse primers located on the flanks of the multiclonal site of the pcDNA3.1 vector. Cell growth rate, hTR expression, telomerase activity and anchorage-independent growth properties were analyzed. RESULTS: Significant downregulation of endogenous hTR was evident in the antisense-hTR transformed cells and telomerase activity was markedly decreased compared to control cells in standard TRAP assays. Furthermore, cell proliferation and the anchorage-independent growth ability in antisense-hTR expressing cells were significantly decreased compared with control parental cells. However, no crisis or senescence phenomena were observed. CONCLUSIONS: These data indicate that hTR may be a critical component of human telomerase activity and suggest that downregulation of the RNA component of human telomerase is a possible target for anticancer strategies.
基金supported by the High Level Talents Introduction Project of Xinjiang Uygur Autonomous Region(No.2013)
文摘The silver （Ag） nanowire arrays with regular and uniform size were successfully fabricated inside the nanochannels of anodic aluminum oxide （AAO） template by a simple paired cell method. X-ray diffi＇action （XRD） and scanning elec- tron microscopy （SEM） results indicate that the as-synthesized samples are composed of face-centered cubic structure, and the average diameter is about 60-70 nm. Transmission electron microscopy （TEM） and the corresponding fast Fourier transformation （FFT） results show that Ag nanowires have a preferred single-crystal structure. Ultravio- let-visible （UV-vis） spectrum of Ag nanowire arrays exhibits UV emission band at 383 nm which can be attributed to the transverse dipole resonance ofAg nanowire arrays. A good surface-enhanced Raman scattering （SERS） spectrum is observed by excitation with a 514.5 nm laser, and the intensity of the SERS peak is about 23 times higher than that of the normal Raman peak measured from an empty AAO template. The high enhancement factor suggests that this method can be used to fabricate SERS sensor with high efficiency.
