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嗜水气单胞菌毒力因子检测及不同毒力基因型菌株对剑尾鱼的致病性研究 被引量:6
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作者 任燕 孙承文 +3 位作者 石存斌 潘厚军 陶家发 吴淑勤 《广东农业科学》 CAS 2015年第17期113-117,共5页
选取嗜水气单胞菌(Aeromonas hydrophila,Ah)的4种主要胞外毒力因子(气溶素、溶血素、胞外蛋白酶和细胞毒性肠毒)设计合成4对引物,对2008—2009年广东、江西两省的临床分离菌株进行检测,评价菌株毒力基因的分布特征及其毒力强弱。60株... 选取嗜水气单胞菌(Aeromonas hydrophila,Ah)的4种主要胞外毒力因子(气溶素、溶血素、胞外蛋白酶和细胞毒性肠毒)设计合成4对引物,对2008—2009年广东、江西两省的临床分离菌株进行检测,评价菌株毒力基因的分布特征及其毒力强弱。60株临床分离株中,具有4种毒力基因(aer+hly A+epa+act+)的菌株占58.33%(35/60),是主要毒力基因型;其他25株的毒力基因有不同数量缺失。不同基因型的代表菌株对剑尾鱼攻击试验结果表明,嗜水气单胞菌毒力因子之间表现出协同作用,aer+hly A+epa+act+型的毒力最强,对剑尾鱼的致死率最高。 展开更多
关键词 嗜水气单胞菌 气溶素 溶血素 胞外蛋白酶 细胞毒性肠毒素 毒力基因 剑尾鱼
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Stimulation of staphylococcal enterotoxin A combined with PML-RARα peptide on the specifical T-cells against NB4 cell line
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作者 Chen Lin Xue Bai +3 位作者 Lijian Yang Shaohua Chen B. N. Selvakumar Yangqiu Li 《The Chinese-German Journal of Clinical Oncology》 CAS 2009年第3期175-177,共3页
Objective: To investigate the effects of staphyococcal enterotoxin A (SEA) on the cytotoxicity of T cells stimulated by PML-RARa peptide in vitro. Methods: Peripheral blood mononuclear cells (MNC) from healthy d... Objective: To investigate the effects of staphyococcal enterotoxin A (SEA) on the cytotoxicity of T cells stimulated by PML-RARa peptide in vitro. Methods: Peripheral blood mononuclear cells (MNC) from healthy donor were obtained by density gradient centrifugation on Ficoll-Hypaque, MNC were cultured with PML-RARa peptide and SEA for 20 days. After induction, the cytotoxicity of T cells induced against NB4 and K562 cell lines were examined by Cell Counting Kit-8 (CCK-8). The CD4 and CD8 surface markers on the harvested CD3^+ T cells were detected by flow cytometry (FCM). Results: The cytotoxicity of T cells induced by PML-RARa peptide with SEA was higher than that of T cells induced only by PML-RARa peptide against NB4 cells. The FCM assay showed that the ratio of CD4^+/CD8^+ T cells were gradually decreased in both groups of PML-RARα peptide whether with SEA or not at the intervals of day 5,10 and 20 day after induction, but the most significantly decreased by PML-RARe peptide with SEA. Conclusion: The specific cytotoxicity of T cells induced by PML-RARa peptide against NB4 cells could be enhanced with superantigen SEA. 展开更多
关键词 SUPERANTIGEN PML-RARa pepUde NB4 cells T cells
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