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首例TCR-T细胞治疗晚期胰腺癌的伦理审查实践 被引量:3
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作者 许卫卫 吉萍 王涛 《中国医学伦理学》 2022年第8期865-869,共5页
回顾性报告北京大学深圳医院伦理委员会对院内开展的首例TCR-T技术治疗晚期胰腺癌个案研究进行伦理评估、讨论、审查和做出决定的情况,讨论的问题包括该案例的性质、风险获益评估、知情同意以及风险控制。最后提出加强创新性治疗研究项... 回顾性报告北京大学深圳医院伦理委员会对院内开展的首例TCR-T技术治疗晚期胰腺癌个案研究进行伦理评估、讨论、审查和做出决定的情况,讨论的问题包括该案例的性质、风险获益评估、知情同意以及风险控制。最后提出加强创新性治疗研究项目的监管,运用医患共同决策模式实行充分有效的知情同意和提升伦理实质审查能力等三个方面的建议,以期为伦理委员会审查细胞治疗等前沿技术的临床研究项目提供参考。 展开更多
关键词 TCR-T 细胞治疗研究 胰腺癌 伦理审查
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Differentiation of hepatocytoid cell induced from whole-bone-marrow method isolated rat myeloid mesenchymal stem cells 被引量:11
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作者 Wei Li Su-Nan Liu Duan-De Luo Lei Zhao Ling-Lan Zeng Shu-Ling Zhang Shu-Li Li 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第30期4866-4869,共4页
AIM: To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC) in vib'o, in order to find suitable resource of hepatocytes for bioartiflcial liver or liver ... AIM: To explore the expansion and differentiation of hepatocytoid cell induced from myeloid mesenchymal stem cell (MSC) in vib'o, in order to find suitable resource of hepatocytes for bioartiflcial liver or liver transplantation. METHODS: The rat myeloid MSC was isolated and divided into three groups which were cultured by Friedensteion method, and then were induced by culture fluid, culture fluid plus cholestatic serum and culture fluid plus hepatocyte growth factor (HGF), respectively. Hepatocytoid cell as well as expression of CK18 and AFP was observed by immunohistochemistry. RESULTS: After the induction for 21 d, hepatocytoid cell was observed, and its expression of CK18 and AFP was detected by immunohistochemistry in MSC cultured with cholestatic serum. Furthermore, on the 35^th d, albumin mRNA was expressed in the cell, suggesting the inducing effect was similar to that by HGF.CONCLUSION: Rat myeloid MSC can differentiate into hepatocyte lineage under appropriate condition. This method is easy to operate. 展开更多
关键词 Mesenchyme stem cell Hepatocytoid cell Expansion DIFFERENTIATION Induction
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Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting 被引量:15
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作者 Han-Tso Lin Shih-Hwa Chiou +6 位作者 Chung-Lan Kao Yi-Ming Shyr Chien-Jen Hsu Yin-Wen Tarng Larry L-T Ho Ching-Fai Kwok Hung-Hai Ku 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第28期4529-4535,共7页
AIM: To isolate putative pancreatic stem cells (PSCs) from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cyto... AIM: To isolate putative pancreatic stem cells (PSCs) from human adult tissues of pancreas duct using serumfree, conditioned medium. The characterization of surface phenotype of these PSCs was analyzed by flow cytometry. The potential for pancreatic lineage and the capability of β-cell differentiation in these PSCs were evaluated as well. METHODS: By using serum-free medium supplemented with essential growth factors, we attempted to isolate the putative PSCs which has been reported to express nestin and pdx-1. The MatrigelTM was employed to evaluate the differential capacity of isolated cells. Dithizone staining, insulin content/secretion measurement, and immunohistochemistry staining were used to monitor the differentiation. Fluorescence activated cell sorting (FACS) was used to detect the phenotypic markers of putative PSCs. RESULTS: A monolayer of spindle-like cells was cultivated. The putative PSCs expressed pdx-1 and nestin. They were also able to differentiate into insulin-, glucagon-, and somatostatin-positive cells. The spectrum of phenotypic markers in PSCs was investigated; a similarity was revealed when using human bone marrow-derived stem cells as the comparative experiment, such as CD29, CD44, CD49, CD50, CD51, CD62E, PDGFR-α, CD73 (SH2), CD81, CD105(SH3). CONCLUSION: In this study, we successfully isolated PSCs from adult human pancreatic duct by using serumfree medium. These PSCs not only expressed nestin and pdx-1 but also exhibited markers attributable to mesenchymal stem cells. Although work is needed to elucidate the role of these cells, the application of these PSCs might be therapeutic strategies for diabetes mellitus. 展开更多
关键词 Putative pancreas stem cell Nestirr pdx-1 Phenotypic marker
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Study of Cellular Experiment of Electric Pulse Imposed on Cancer Cell 被引量:1
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作者 熊兰 《Journal of Chongqing University》 CAS 2002年第1期53-55,共3页
The objective of the study is the cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3. Ovarian cancer cell suspension were treated by electric pulse with different parameters... The objective of the study is the cytocidal and inhibitory effect of energy-controllable pulse on ovarian cancer cell line SKOV3. Ovarian cancer cell suspension were treated by electric pulse with different parameters. The inhibitory rate (IR) was assayed by modified colorimetric MTT methods, the growth curves of two test groups and one control group were also measured, and the ultrastructural changes were observed under electron microscopy (EM) and scan electron microscopy (SEM). It was found that the treated SKOV3 cell proliferated more slowly. IR was increased with the enhancement of pulse parameters. The ultrastructural study showed that morphological changes occurred obviously. Swollen mitochondria, fractured ridges, cyto-plasmic vacuoles and membrane holes appeared in most of the processed cells, and a part of bilayer membrane was ruptured. It is indicated that irreversible electric breakdown occurred in some of the treated cells, and the electric pulse could kill cancer cell and inhibit its recovery and growth. 展开更多
关键词 Energy-controllable pulse Irreversible breakdown Inhibitory rate(IR) Proliferation inhibition
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The human leucocyte differentiation antigens (HLDA) workshops: the evolv-ing role of antibodies in research, diagnosis and therapy 被引量:2
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作者 Heddy ZOLA Bernadette SWART 《Cell Research》 SCIE CAS CSCD 2005年第9期691-694,共4页
The 8^th International Workshop on Human Leucocyte Differentiation Antigens (chaired by HZ and managed by BS) was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievem... The 8^th International Workshop on Human Leucocyte Differentiation Antigens (chaired by HZ and managed by BS) was run over a 4-year period and culminated in a conference in December 2004. Here we review the achievements of the HLDA Workshops and provide links to information on CD molecules and antibodies against them, including the 93 new CDs assigned in the 8^th Workshop. We consider what remains to be achieved (including an estimate of the number of leucocyte surface molecules still to be discovered), and how the field can best move forward. 展开更多
关键词 leucocyte differentiation antigens CD molecules cell markers
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Influence of comorbidity on the choice of treatment and survival of elderly patients with advanced non-small cell lung cancer
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作者 Aiwu Li Caicun Zhou Jianfang Xu Ren Zhu Jie Luo Shanhao Chen 《The Chinese-German Journal of Clinical Oncology》 CAS 2008年第5期259-262,共4页
Objective: To identify the influence of comorbidity on the choice of treatment and survival of elderly patients (≥70 years) with advanced non-small cell lung cancer (NSCLC). Methods: The clinical characteristic... Objective: To identify the influence of comorbidity on the choice of treatment and survival of elderly patients (≥70 years) with advanced non-small cell lung cancer (NSCLC). Methods: The clinical characteristics and the choices of treatment of 177 elderly patients, who had a good performance status (PS≤1) were retrospectively analyzed in Oncology Department, Shanghai Pulmonary Hospital, between January 2005 to December 2005. Survival data were only analyzed in those whose had received chemotherapy. All patients were stratified by number of comorbidity as none (0), mild (1-2) and severe (≥ 3) groups. Results: The proportion of patients, who received chemotherapy, with none, mild and severe comorbidity was significantly different (79.3%, 76.2% and 57.4%, P=0.038), and there was also significantly different about palliative radiotherapy rate among the three groups (21.7%, 11.7% and 37.0%, P=0.014). The median survival and 1-year survival rate in none, mild and severe comorbidity groups, were 13.6 vs. 10.2 vs. 7.6 months and 53.5% vs. 41.3% vs. 20.8% respectively (Log-rank, P=0.071). In univariate and multivariate Cox models analysis, only severe comorbidity was a independent hazard factor of survival of elderly patients with NSCLC. Relative ratio (RR, 95% CI): (2.09, 1.06-4.15), P=0.034. Conclusion: Comorbidity may affect the choice of treatment of elderly patients with advanced NSCLC slightly, but only severe comorbidity is a independent prognostic factor of survival. 展开更多
关键词 ELDERLY non-small cell lung cancer COMORBIDITY
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