Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to th...Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.展开更多
Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular mechanisms of appressorium formation to restrain this fungal pathogen. Auto...Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular mechanisms of appressorium formation to restrain this fungal pathogen. Autophagy is a very high conserved process in eukaryotic cells. Recently, autophagy has been considered as a key process in development and differentia-tion in M. oryzae. In this report, we present and discuss the current state of our knowledge on gene expression in appressorium formation and the progress in autophagy of rice blast fungi.展开更多
AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34...AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS: Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 ± 1.6 d vs 11.4 ± 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 ± 1.4 generations vs 12.3 ± 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm. CONCLUSION: MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.展开更多
On Nov 17th,a team of researchers from the Shanghai Institute of Biochemistry and Cell Biology（SIBCB）,Shanghai Institutes for Biological Sciences,CAS led by Prof.LI Jinsong reports online in Cell Research a novel te...On Nov 17th,a team of researchers from the Shanghai Institute of Biochemistry and Cell Biology（SIBCB）,Shanghai Institutes for Biological Sciences,CAS led by Prof.LI Jinsong reports online in Cell Research a novel technique to induce from mice oocytes haploid embryonic stems cells（haESCs）that can fully replace the reproductive functions of sperms,greatly simplifying the otherwise complicated techniques to produce such stem cells and semi-cloned（SC）mice.It is anticipated that this will further facilitate research in the field of stem cells and embryonic development;展开更多
Dr.Xiyun Yan obtained her B.S.degree in 1983,from the Henan Medical College,and then studied cell biology in Professor Shizhang Bei’s lab in the Institute of Biophysics,Chinese Academy of Sciences（CAS）until leaving...Dr.Xiyun Yan obtained her B.S.degree in 1983,from the Henan Medical College,and then studied cell biology in Professor Shizhang Bei’s lab in the Institute of Biophysics,Chinese Academy of Sciences（CAS）until leaving for Germany in 1989.After receiving her medical doctor＇s degree from Heidelberg University in Germany,she joined the Memorial Sloan-Kettering Institute in New York as a postdoctoral research fellow.In 1997,展开更多
基金Supported by the Special Financial Program of Hebei Province,the Fund for Agricultural Science and Technology Innovation Talent Team Construction(2011055001)~~
文摘Coix has a long cultivation history as a minor crop in China, but many problems including unclear origin, chaos of species, loss and inaccurate use of germplasm resources exist in its research and production due to the lack of systematic study. In recent years, the importance of Coix has been realized with the exploration of its effects in treating and preventing cancer. The systematic study of Coix has met a superb development opportunity with the rapid development of biotechnology especially the deep-sequencing technique. Therefore, this paper presented here the current status of origin and evolution, classification, cytology and molecular biology progress of Coix, analyzed the ploidy of Coix and the genetic relationships with Zea, Sorghum and so on from the point of cytology, and then based on the molecular biology, analyzed the important significance of DNA molecular marker, genetic mapping and gene library in the research on identification of genetic relationship between species and genetic breeding, which could provide useful information for further Coix research.
基金the National Natural Science Foundation of China (Nos. 30671351 and 30870101)the Hi-Tech Research and Development Program (863) of China (No. 2002AA245041)
文摘Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular mechanisms of appressorium formation to restrain this fungal pathogen. Autophagy is a very high conserved process in eukaryotic cells. Recently, autophagy has been considered as a key process in development and differentia-tion in M. oryzae. In this report, we present and discuss the current state of our knowledge on gene expression in appressorium formation and the progress in autophagy of rice blast fungi.
基金Technology Project Fund of Guangdong Province, No. 2003A3020303
文摘AIM: To establish a culture system of marrow mesenchymal stem cells (MSCs)from hepatitis B patients and normal adults and to compare their biological characteristics. METHODS: MSCs were isolated from bone marrow in 34 male hepatitis B patients and 15 male normal adults and cultivated in vitro. Their biological characteristics including surface markers, shapes and appearances, growth curves, first passage time and passage generations were compared. RESULTS: Cultivation achievement ratio of hepatitis B patients was lower than that of normal adults, no statistical significance (82.35% vs 100%, P > 0.05). Compared with MSCs of normal adults, MSCs of hepatitis B patients presented a statistical lower growth curve, longer first passage time (13.0 ± 1.6 d vs 11.4 ± 1.5 d, P < 0.05), fewer passaging generation numbers (10.5 ± 1.4 generations vs 12.3 ± 1.7 generations, P < 0.05), though both shared same appearances, shapes and surface markers. MSCs in hepatitis B patients would expand, spread out and age more easily and there were more refractive particles in the cytoplasm. CONCLUSION: MSCs from hepatitis B patients can be cultured in vitro. Although their appearance, shape and surface marker are similar to those of MSCs from normal adults, there are differences in their biological characteristics.
文摘On Nov 17th,a team of researchers from the Shanghai Institute of Biochemistry and Cell Biology（SIBCB）,Shanghai Institutes for Biological Sciences,CAS led by Prof.LI Jinsong reports online in Cell Research a novel technique to induce from mice oocytes haploid embryonic stems cells（haESCs）that can fully replace the reproductive functions of sperms,greatly simplifying the otherwise complicated techniques to produce such stem cells and semi-cloned（SC）mice.It is anticipated that this will further facilitate research in the field of stem cells and embryonic development;
文摘Dr.Xiyun Yan obtained her B.S.degree in 1983,from the Henan Medical College,and then studied cell biology in Professor Shizhang Bei’s lab in the Institute of Biophysics,Chinese Academy of Sciences（CAS）until leaving for Germany in 1989.After receiving her medical doctor＇s degree from Heidelberg University in Germany,she joined the Memorial Sloan-Kettering Institute in New York as a postdoctoral research fellow.In 1997,
