Two new types of lentiviral vectors expressing a reporter transgene encoding either firefly luciferase （fLuc） for bioluminescence imaging or the HSV1 thymidine kinase （HSV1-TK） for radiopharmaceutical-based imagin...Two new types of lentiviral vectors expressing a reporter transgene encoding either firefly luciferase （fLuc） for bioluminescence imaging or the HSV1 thymidine kinase （HSV1-TK） for radiopharmaceutical-based imaging were constructed to monitor human embryonic stem cell （hESC） engraftment and proliferation in live mice after trans- plantation. The constitutive expression of either transgene did not alter the properties of hESCs in the culture. We next monitored the formation of teratomas in SCID mice to test （1） whether the gene-modified hESCs maintain their developmental pluripotency, and （2） whether sustained reporter gene expression allows noninvasive, whole-body imaging of hESC derivatives in a live mouse model. We observed teratoma formation from both types of gene-modified cells as well as wild-type hESCs 2-4 months after inoculation. Using an optical imaging system, bioluminescence from the fLuc-transduced hESCs was easily detected in mice bearing teratomas long before palpable tumors could be detected. To develop a noninvasive imaging method more readily translatable to the clinic, we also utilized HSV1-TK and its specific substrate, 1-（2＇-deoxy-2＇-fluoro-β-D-arabinofuranosyl）-5-[^125I]iodouracil（[^125I]FIAU）, as a reporter/ probe pair. After systemic administration, [^125I]FIAU is phosphorylated only by the transgene-encoded HSV1-TK enzyme and retained within transduced （and transplanted） cells, allowing sensitive and quantitative imaging by single-photon emission computed tomography. Noninvasive imaging methods such as these may enable us to monitor the presence and distribution of transplanted human stem cells repetitively within live recipients over a long term through the expression of a reporter gene.展开更多
Gold nanoparticles(Au NPs) have been extensively used in optical biosensing and bioimaging due to the unique optical properties. Biological applications including biosensing and cellular imaging based on optical prope...Gold nanoparticles(Au NPs) have been extensively used in optical biosensing and bioimaging due to the unique optical properties. Biological applications including biosensing and cellular imaging based on optical properties of Au NPs will be reviewed in the paper. The content will focus on detection principles, advantages and challenges of these approaches as well as recent advances in this field.展开更多
Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent yea...Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent years,single plasmonic nanoparticle sensors have endowed a new dimension to the imaging scale given their comparable size to biomolecules such as nucleic acids or antibodies.展开更多
A curcumin derivative ligand,1,7-bis(3-methoxyl-4-acetoxyl)phenyl-1,6-heptadiene-3,5-diketone (diacetylcurcumin,abbreviated as HL),and its Cu and Ni complexes have been synthesized and fully characterized by elemental...A curcumin derivative ligand,1,7-bis(3-methoxyl-4-acetoxyl)phenyl-1,6-heptadiene-3,5-diketone (diacetylcurcumin,abbreviated as HL),and its Cu and Ni complexes have been synthesized and fully characterized by elemental analyses,IR,1 HNMR and molar conductivity.The resulting complexes exhibit two-photon excited fluorescence (TPEF) in DMF,and have been proven to be potentially useful for two-photon microscopy imaging in living cells.In addition,cytotoxicity tests showed that the low-micromolar concentrations of ML 2 did not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.展开更多
Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficac...Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentra- tion of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania （i.e., nano-TiO2） may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin （TSPP） with nano-titania （PT） for efficient PDT with least adverse effects. The spectroscopic properties of these nano-cornposites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was deter- mined by using 2,5-diphenyl-3,4-benzofuran （DPBF）, while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human （HSC） and rat models （RSC） were investigated by confocal laser microscopy and 3-（4,5-dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effec- tive PDT treatment of Rheumatoid Arthritis （RA）.展开更多
文摘Two new types of lentiviral vectors expressing a reporter transgene encoding either firefly luciferase （fLuc） for bioluminescence imaging or the HSV1 thymidine kinase （HSV1-TK） for radiopharmaceutical-based imaging were constructed to monitor human embryonic stem cell （hESC） engraftment and proliferation in live mice after trans- plantation. The constitutive expression of either transgene did not alter the properties of hESCs in the culture. We next monitored the formation of teratomas in SCID mice to test （1） whether the gene-modified hESCs maintain their developmental pluripotency, and （2） whether sustained reporter gene expression allows noninvasive, whole-body imaging of hESC derivatives in a live mouse model. We observed teratoma formation from both types of gene-modified cells as well as wild-type hESCs 2-4 months after inoculation. Using an optical imaging system, bioluminescence from the fLuc-transduced hESCs was easily detected in mice bearing teratomas long before palpable tumors could be detected. To develop a noninvasive imaging method more readily translatable to the clinic, we also utilized HSV1-TK and its specific substrate, 1-（2＇-deoxy-2＇-fluoro-β-D-arabinofuranosyl）-5-[^125I]iodouracil（[^125I]FIAU）, as a reporter/ probe pair. After systemic administration, [^125I]FIAU is phosphorylated only by the transgene-encoded HSV1-TK enzyme and retained within transduced （and transplanted） cells, allowing sensitive and quantitative imaging by single-photon emission computed tomography. Noninvasive imaging methods such as these may enable us to monitor the presence and distribution of transplanted human stem cells repetitively within live recipients over a long term through the expression of a reporter gene.
基金supported by the National Natural Science Foundation of China (Nos. 21527810, 21190041, 21307029, and 21221003)
文摘Gold nanoparticles(Au NPs) have been extensively used in optical biosensing and bioimaging due to the unique optical properties. Biological applications including biosensing and cellular imaging based on optical properties of Au NPs will be reviewed in the paper. The content will focus on detection principles, advantages and challenges of these approaches as well as recent advances in this field.
文摘Revealing the behavior of single molecules in single live cells provides a fundamental approach to understand cellular organization and dynamics.With the rapid merging of biotechnology and nanotechnology in recent years,single plasmonic nanoparticle sensors have endowed a new dimension to the imaging scale given their comparable size to biomolecules such as nucleic acids or antibodies.
基金supported by the National Natural Science Foundation of China (21071001)Department of Education Committee of Anhui Province (KJ2010A222)the Natural Science Foundation of Anhui Unversity of Traditional Chinese Medicine (2011zr005A)
文摘A curcumin derivative ligand,1,7-bis(3-methoxyl-4-acetoxyl)phenyl-1,6-heptadiene-3,5-diketone (diacetylcurcumin,abbreviated as HL),and its Cu and Ni complexes have been synthesized and fully characterized by elemental analyses,IR,1 HNMR and molar conductivity.The resulting complexes exhibit two-photon excited fluorescence (TPEF) in DMF,and have been proven to be potentially useful for two-photon microscopy imaging in living cells.In addition,cytotoxicity tests showed that the low-micromolar concentrations of ML 2 did not cause significant reduction in cell viability over a period of at least 24 h and should be safe for further biological studies.
基金supported by the National Natural Science Foundation of China (81325011)National High Technology Research & Development Program of China (2015AA020502, 2012AA022703)+1 种基金the Major Science & Technology Project of Suzhou (ZXY2012028)the National Science Foundation CREST program (NSF HRD-0932421)
文摘Photodynamic therapy （PDT） is one of the latest biomedical technologies used for treatment of various neoplastic and non-neoplastic diseases. However, there still exist some well-known problems regarding its efficacy, e.g. effective concentra- tion of the drug at the desired sites, the irradiation light dosimetry and biocompatibility of the photosensitizer. The introduction of nanotechnology and nanomaterial like biocompatible nano-titania （i.e., nano-TiO2） may facilitate to solve some of these problems. In this study we have explored the possibility of combining tetra sulphonatophenyl porphyrin （TSPP） with nano-titania （PT） for efficient PDT with least adverse effects. The spectroscopic properties of these nano-cornposites were characterized by using fluorescence and UV-Vis absorption spectroscopic study. The singlet oxygen quantum yield was deter- mined by using 2,5-diphenyl-3,4-benzofuran （DPBF）, while the effect of nano TiO2 with TSPP on the synovial fibroblast cells from human （HSC） and rat models （RSC） were investigated by confocal laser microscopy and 3-（4,5-dimethylthiazol-2-yl）- 2,5-diphenyltetrazolium bromide （MTT） assay. Our results suggest that nano TiO2 with TSPP can be readily utilized for effec- tive PDT treatment of Rheumatoid Arthritis （RA）.
