The myeloperoxidase (MPO) is an important microbicidal protein present at high concentration in the primary granule of mature granulocyte and its expression is regulated in both myeloidcell-lineage and premyelocytic-s...The myeloperoxidase (MPO) is an important microbicidal protein present at high concentration in the primary granule of mature granulocyte and its expression is regulated in both myeloidcell-lineage and premyelocytic-stagespecific manners. A better understanding of the underlying control mechanisms should provide insights into the temporal and co-ordinate regulation of the gene expression during granulopoiesis. We have identified its promoter by mapping the start(s) of transcription using various molecular approaches together with demonstrating the promoter function of the relevant DNA segment in a transient transfection reporter assay. Besides the major start of transcription mapped at G residue, 11 nucleotide upstream of the 3’ end of exon 0, the usage of that is specific to the MPO expressing cell lines, we have shown that irrespective of the MPO-expression status of the hematopoietic cells, transcription occurs broadly within a two kb region upstream of the 5’ proximity of the gene, and is largely terminated in nitron 2. These data support a model of the pre myelocytic-stage-specific MPO expression, the control of which is operated at initiation as well as elongation levels of transcription.展开更多
Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the GI globular domain of the aggrecan (GI) contained the arthritogenic region...Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the GI globular domain of the aggrecan (GI) contained the arthritogenic region. To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients, we analyzed the frequency of human G1-specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1-reactive T cell lines from these rheumatoid arthritis patients. The results showed that the G1-specific T cells in PBL were detectable at the range of 4.97 ±0.5 ×10-6 in peripheral blood lymphocytes. We have also generated 15 G1-specific T lymphocyte lines from these pateints with a standard split-well method. All these cells expressed fine specificity to human recombinant G1, but not to unrelated antigen. All the 15 lines expressed a panT cell marker and 13 of them selectively used the αβ T cell receptor. Two of them used rye T cell receptor. The 13 of these T cell lines was CD4 positive. One line expressed CD8. One line expressed both CD4 and CD8. Moreover, 14 out of 15 lines expressed the Th-1 cytokine profile, characterized by interferon-γpositivity and IL-4 negativity. No Th-2 type cell line was generated. These data provide strong evidence in favor of the presence of autoreactive T cells in the rheumatoid arthritis pateints. What is the mechanism(s) that these autoreactive T cells attack self-target and whether these G1-specific, Th-1 type T cell lines can induce arthritis in immune deficiency mice are currently under investigation.展开更多
OBJECTIVE A system was established to evaluate the metastasis and prognosis of oral squamous cell carcinoma by analyzing the tumor differentiation, the TNM stage, the mode of invasion, and the expression of E-cadherin...OBJECTIVE A system was established to evaluate the metastasis and prognosis of oral squamous cell carcinoma by analyzing the tumor differentiation, the TNM stage, the mode of invasion, and the expression of E-cadherin and S100A4. METHODS Squamous cell carcinoma of the oral cavity of 86 cases was the focus of our study. In this system, the histopathological grade and the histochemical patterns were estimated on a 0-3 point scale, the total points graded from 0 to 13. RESULTS The incidence of metastasis and prognosis in the cases with total points more than 8 was significantly higher than that with total points less than 7 （P 〈 0.05,χ^2= 22.0658 and P 〈 0.05, χ^2= 10.7047）. The system had a significant higher specificity than that of ＇DIAGS index＇ system （Differentiation, Invasion mode, Adhesion molecules, Glycosaminoglycan, and the Sugar chain） in the evaluation of metastasis （P 〈 0.05, u = 2.2339）. Moreover, the specificity for evaluation of metastasis in the system was significantly higher than that of E-cadherin （P 〈 0.05, u = 2.4996） or S100A4 （P 〈 0.05, u = 2.4289） only. Furthermore the specificity for evaluation of unfavorable prognosis in the system was also significantly higher than that of E-cadherin （P 〈 0.05, u = 2.1313） or S100A4 only （P 〈 0.05, u = 2.0301）. CONCLUSION This is a valuable evaluation system with high specificity to predict metastatic potential and prognosis of oral squamous cell carcinoma.展开更多
文摘The myeloperoxidase (MPO) is an important microbicidal protein present at high concentration in the primary granule of mature granulocyte and its expression is regulated in both myeloidcell-lineage and premyelocytic-stagespecific manners. A better understanding of the underlying control mechanisms should provide insights into the temporal and co-ordinate regulation of the gene expression during granulopoiesis. We have identified its promoter by mapping the start(s) of transcription using various molecular approaches together with demonstrating the promoter function of the relevant DNA segment in a transient transfection reporter assay. Besides the major start of transcription mapped at G residue, 11 nucleotide upstream of the 3’ end of exon 0, the usage of that is specific to the MPO expressing cell lines, we have shown that irrespective of the MPO-expression status of the hematopoietic cells, transcription occurs broadly within a two kb region upstream of the 5’ proximity of the gene, and is largely terminated in nitron 2. These data support a model of the pre myelocytic-stage-specific MPO expression, the control of which is operated at initiation as well as elongation levels of transcription.
文摘Our previous work showed that the cartilage proteoglycan aggrecan could induce an erosive polyarthritis and spondylitis in BALB/c mice and the GI globular domain of the aggrecan (GI) contained the arthritogenic region. To elucidate whether autoreactive T cells to G1 are expressed in rheumatoid arthritis patients, we analyzed the frequency of human G1-specific T cells in the peripheral blood of five rheumatoid arthritis patients and tried to establish G1-reactive T cell lines from these rheumatoid arthritis patients. The results showed that the G1-specific T cells in PBL were detectable at the range of 4.97 ±0.5 ×10-6 in peripheral blood lymphocytes. We have also generated 15 G1-specific T lymphocyte lines from these pateints with a standard split-well method. All these cells expressed fine specificity to human recombinant G1, but not to unrelated antigen. All the 15 lines expressed a panT cell marker and 13 of them selectively used the αβ T cell receptor. Two of them used rye T cell receptor. The 13 of these T cell lines was CD4 positive. One line expressed CD8. One line expressed both CD4 and CD8. Moreover, 14 out of 15 lines expressed the Th-1 cytokine profile, characterized by interferon-γpositivity and IL-4 negativity. No Th-2 type cell line was generated. These data provide strong evidence in favor of the presence of autoreactive T cells in the rheumatoid arthritis pateints. What is the mechanism(s) that these autoreactive T cells attack self-target and whether these G1-specific, Th-1 type T cell lines can induce arthritis in immune deficiency mice are currently under investigation.
基金supported by a grant from the Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars,State Education Ministry,China(No.2005383).
文摘OBJECTIVE A system was established to evaluate the metastasis and prognosis of oral squamous cell carcinoma by analyzing the tumor differentiation, the TNM stage, the mode of invasion, and the expression of E-cadherin and S100A4. METHODS Squamous cell carcinoma of the oral cavity of 86 cases was the focus of our study. In this system, the histopathological grade and the histochemical patterns were estimated on a 0-3 point scale, the total points graded from 0 to 13. RESULTS The incidence of metastasis and prognosis in the cases with total points more than 8 was significantly higher than that with total points less than 7 （P 〈 0.05,χ^2= 22.0658 and P 〈 0.05, χ^2= 10.7047）. The system had a significant higher specificity than that of ＇DIAGS index＇ system （Differentiation, Invasion mode, Adhesion molecules, Glycosaminoglycan, and the Sugar chain） in the evaluation of metastasis （P 〈 0.05, u = 2.2339）. Moreover, the specificity for evaluation of metastasis in the system was significantly higher than that of E-cadherin （P 〈 0.05, u = 2.4996） or S100A4 （P 〈 0.05, u = 2.4289） only. Furthermore the specificity for evaluation of unfavorable prognosis in the system was also significantly higher than that of E-cadherin （P 〈 0.05, u = 2.1313） or S100A4 only （P 〈 0.05, u = 2.0301）. CONCLUSION This is a valuable evaluation system with high specificity to predict metastatic potential and prognosis of oral squamous cell carcinoma.
