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Phylogenetic Relationships of Monal Pheasants Lophophorus Inferred from Sequences of Mitochondrial Cytochrome b Gene 被引量:13
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作者 詹祥江 张正旺 +1 位作者 吴爱平 陶玉静 《Zoological Research》 CAS CSCD 北大核心 2003年第5期337-342,共6页
The phylogeny of the monal pheasants (Lophophorus) and their relationships to some species of the genera Tragopan,Pucrasia and Ithaginis were studied by comparing mitochondrial cytochrome b (cyt b) nucleotide sequence... The phylogeny of the monal pheasants (Lophophorus) and their relationships to some species of the genera Tragopan,Pucrasia and Ithaginis were studied by comparing mitochondrial cytochrome b (cyt b) nucleotide sequences.The molecular phylogenetic trees show that:①the genus Tragopan and the genus Pucrasia share a common ancestor which is the sister taxon of the ancestor of the genus Lophophorus;②the genus Lophophorus had evolved into two branches:One was the Sclaters Monal;the other included the Chinese Monal and the Himalayan Monal.Considering its molecular phylogeny,distribution patterns and morphological evidences,the genus Lophophorus might originate in the Hengduan mountains region of southwestern China. 展开更多
关键词 Lophophorus PHEASANT PHYLOGENY Cyt b gene
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Identification of sika deer and red deer using partial cytochrome b and 12s ribosomal RNA genes 被引量:7
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作者 李波 白素英 +2 位作者 徐艳春 张伟 马建章 《Journal of Forestry Research》 SCIE CAS CSCD 2006年第2期160-162,共3页
A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes s... A study was conducted on the identifications of the degraded samples of sika deer (Cervus nippon) and red deer (Cervus elaphus) by phylogenetic and nucleotide distance analysis of partial Cytb and 12s rRNA genes sequences. 402 bp Cytb genes were achieved by PCR-sequencing using DNA extracted from 8 case samples, and contrasted with 27 sequences of Cytb gene downloaded from GenBank database. The values of three nucleotide distance between three suspected samples and sika deer were identical (0.026±0.006), which was smaller than the smallest nucleotide distance between eastern red deer and sika deer (0.036). Furthermore, phylogenetic analysis of sika deer and red deer indicated that the evidences located within the same cluster as sika deer. The evidences were sika deer materials. As the same way, other three suspected samples were derived from red deer. The results were further confirmed by phylogenetic and nucleotide distance analysis of 387 bp 12s rRNA gene. The method was powerful and less time-consuming and helpful to reduce the related cases with wildlife. 展开更多
关键词 Sika deer (Cervus nippon) Red deer (Cervus elaphus) Cytochrome b gene (Cytb 12s ribosomal RNA gene (12s rRNA)
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DGGE Analysis on Mitochondrial Cyt b Gene of Eriocheir sinensis and Eriocheir hepuensis 被引量:2
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作者 王晓梅 李晓东 +1 位作者 王茜 靳婷婷 《Agricultural Science & Technology》 CAS 2011年第6期890-892,907,共4页
[Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis(DGGE).[Method] Mit... [Objective] The aim was to investigate the possibility to analyze the genetic diversity of Eriocheir sinensis and Eriocheir hepuensis by using the technique denaturing gradient gel electrophoresis(DGGE).[Method] Mitochondrial cyt b gene fragment was amplified from 180 individuals of five populations of E.sinensis and a population of E.hepuensis and then analyzed by using DGGE.[Result] All PCR products showed two kinds of electrophoretic mobility on DGGE.The PCR products of all individuals from E.hepuensis showed the same mobility with that of the individuals from 46.7% of Jiangdu population,23.3% of Yizheng population and 20.0% of Wenzhou population of E.sinensis,while the rest of the individuals from the three populations of E.sinensis mentioned above as well as all the individuals of Nanjing and Panjin populations showed the same mobility,which was higher compared with that of E.hepuensis.The results indicated that there was the same genetic marker in E.sinensis populations as that of E.hepuensis population,which was consistent with previous studies.[Conclusion] DGGE technique could be used to analyze the genetic diversity of Chinese mitten crab. 展开更多
关键词 E.sinensis E.hepuensis Mitochondrial cyt b gene fragment DGGE
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Phylogenetic Relationships of 11 Bumblebee Species (Hymenoptera:Apidae) Based on Mitochondrial Cytochrome b Gene Sequences 被引量:7
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作者 邵志勇 茅红新 +1 位作者 符文俊 张亚平 《Zoological Research》 CAS CSCD 北大核心 2002年第5期361-366,共6页
Phylogenetic relationships of 11 bumblebee species,including 5 subgenera:Bombus (5 species),Thoracobombus (3 species),Mendacibombus (1 species),Fervidobombus (1 species) and Pyrobombus (1 species),were analyzed based ... Phylogenetic relationships of 11 bumblebee species,including 5 subgenera:Bombus (5 species),Thoracobombus (3 species),Mendacibombus (1 species),Fervidobombus (1 species) and Pyrobombus (1 species),were analyzed based on the 357?bp mitochondrial cytochrome b gene sequences.There are 65 singleton polymorphic sites and 71 parsimony informative polymorphic sites in this DNA segment,and 45 polymorphic sites within the total 119 translated amino acids segment.Both NJ tree and MP tree show that Mendacibombus (B.avinovielllus) is basal to others,followed by Fervidobombus (B.pensylvanicus);Pyrobombus (B.impatiens) and Bombus are sister subgenera;the subgenus of Bombus is monophyletic,in which B.ignitus diverged first. 展开更多
关键词 bOMbUS Cytochrome b gene DNA sequence Amino acid sequence Molecular phylogeny
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Sequence Comparison of Partial Cytochrome b Genes of Two Coilia species 被引量:10
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作者 GAOTianxiang WANGYujiang ZHANGYaping 《Journal of Ocean University of China》 SCIE CAS 2005年第1期85-88,共4页
Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substituti... Sequence variation of partial cytochrome b genes between two Coilia species, C. ectenes and C. mystus, was in- vestigated. Of the 402 nucleotides, twenty-seven (6.72%) are polymorphic and all are synonymous substitutions. At the third positions of genetic condon of cytochrome b gene, the two species show an extreme anti-G bias (<4%) and a pronounced bias towards A and C (>68%). There is no amino acid sequence divergence between the partial cytochrome b genes of the two species, indicating a close genetic relationship between them. The k-2p genetic distance of partial cytochrome b segment of the two species is 0.072, suggesting that the species were separated 3.6 Ma ago, in the middle Pliocene. Our result reveals that the cytochrome b gene is an appropriate marker for studies of population genetic structures and phylogeographic pat- terns of the two species. 展开更多
关键词 cytochrome b gene genetic variation mitochondrial DNA Coilia ectenes Coilia mystus
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Molecular Phylogeny of Slow Lorises (Nycticebus) Revealed by D-loop Sequences and Complete Cytochrome b Gene Sequences of Mitochondrial DNA
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作者 陈静华 Paul CRO W +2 位作者 成岛悦雄 张红卫 张亚平 《Zoological Research》 CAS CSCD 北大核心 2004年第4期292-297,共6页
Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.S... Partial sequences of the D-loop and the complete sequences of cytochrome b gene (1 140 bp) of the slow lorises (genus Nycticebus) were undertaken to investigate evolutionary relationships among species of Nycticebus.Sequence analysis results consistently provide new taxonomy evidence at the DNA level for supporting Ratajszczak and Groves’ viewpoint that N.intermedus is merely the adult of N.pygmaeus (Ratajszczak,1998;Groves,1971).Phylogenetic analysis was performed by means of the combined data and these two separate sequences data,respectively,by using various methods,supporting the same topology,in which genus Nycticebus was formed of two clusters.The first cluster was composed of N.pygmaeus,and the second cluster of N.coucang.It also could provide a new molecular genetic evidence to support the view that the genus comprises two species:N.coucang and N.pygmaeus. 展开更多
关键词 Nycticebus Mitochondiral DNA Cytochrome b gene D-LOOP Molecular phylogeny
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Cloning, characterization, and expression of Cytochrome b (Cytb)——a key mitochondrial gene from Prorocentrum donghaiense 被引量:2
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作者 赵丽媛 米铁柱 +1 位作者 甄毓 于志刚 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2012年第3期424-432,共9页
Mitochondrial cytochrome b (Cytb), one of the few proteins encoded by the mitochondrial DNA, plays an important role in transferring electrons. As a mitochondrial gene, it has been widely used for phylogenetic analy... Mitochondrial cytochrome b (Cytb), one of the few proteins encoded by the mitochondrial DNA, plays an important role in transferring electrons. As a mitochondrial gene, it has been widely used for phylogenetic analysis. Previously, a 949-bp fragment of the coding gene and mRNA editing were characterized from Prorocentrum donghaiense, which might prove useful for resolving P. donghaiense from closely related species. However, the full-length coding region has not been characterized. Ih this study, we used rapid amplification of cDNA ends (RACE) to obtain full-length, 1 124 bp cDNA. Cytb transcript contained a standard initiation codon ATG, but did not have a recognizable stop codon. Homology comparison showed that the P. donghaiense Cytb had a high sequence identity to Cytb sequences from other dinoflagellate species. Phylogenetic analysis placed Cytb from P. donghaiense in the clade of dinoflagellates and it clustered together strongly with that from P. minimum. Based on the full-length sequence, we inferred 32 editing events at different positions, accounting for 2.93% of the Cytb gene. 34.4% (11) of the changes were A to G, 25% (8) were T to C, and 25% (8) were C to U, with smaller proportions of G to C and G to A edits (9.4% (3) and 6.2% (2), respectively). The expression level of the Cytb transcript was quantified by real-time PCR with a TaqMan probe at different times during the whole growth phase. The average Cytb transcript was present at 39.277.46 copies of cDNA per cell during the whole growth cycle, and the expression of Cytb was relatively stable over the different phases. These results deepen our understanding of the structure and characteristics of Cytb in P. donghaiense, and confirmed that Cytb in P. donghaiense is a candidate reference gene for studying the expression of other genes. 展开更多
关键词 Cytochrome b (Cytb Prorocentrum donghaiense real-time PCR red tide reference gene 'RNA editing
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Rapid and Sensitive Identification of Horse and Donkey Meat in Iraqi Markets Using SSR and PCR-RFLP Based on Mitochondrial DNA Cytochrome B Gene 被引量:3
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作者 Talib Ahmed Jaayid 《Journal of Agricultural Science and Technology(A)》 2013年第11期896-903,共8页
This study was carried out at the Genetic Engineering Lab, College of Agriculture, University of Basrah. DNA was extracted from samples containing meat of commercial products in the Basrah markets. The products subjec... This study was carried out at the Genetic Engineering Lab, College of Agriculture, University of Basrah. DNA was extracted from samples containing meat of commercial products in the Basrah markets. The products subjected to analysis using polymerase chain reaction (PCR) with species specific repeat (SSR) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques based on the sequence of the mitochondrial DNA cytochrome b gene (mtDNA cyt b gene). Then digestion with the Alu l restriction enzyme to establish a differential diagnosis detects and discriminates between meat species and adulteration in the products. SSR primers were applied, has been detected amplification of the encoded gene product, generated 221 bp in some imported minced and canned meat samples. The results show that SSR analysis produced a pattern that allowed a direct identification of horse and donkey meats in some imported minced and canned meat samples (Hana, Monde and Bavaria). The amplified 359 bp gene of mtDNA cyt b gene from samples in different product was cut using Alu 1 restriction enzyme resulting in restriction fragment length polymorphism (RFLP). Alu 1 was used to distinguish between the animals meat that belong to the family or one species. The digestion of the PCR product showed differences between products. Where the fragment length generated were 74, 76 and 189 bp. It belonged to horse meat. The fraud was detected in Hana, Monde and Bavaria products available in Basrah markets showing the presence of horse meat in these products that labeled as beef meats 100%. This revealed mtDNA cyt b gene as highly conserved and consequently a good molecular marker for diagnostic studies. Thus, this technique can be applied to food authentication for the identification of different species of animals in food products. 展开更多
关键词 Polymerase chain reaction SSR RFLP farm animal meats identification adulteration.
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Preliminary Study on Geographical Variation of Cytochrome b gene and ITS2-rDNA among Populations of Rhynchophorus ferrugineus 被引量:1
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作者 R. A. A. M. EI-Mergawy A. M. A1 Ajlan +5 位作者 N. Abdalla V. Vassiliou C. Capdevielle-Dulac D.C.Kontodimas J. F. Silvain M. I. Nasr 《Journal of Agricultural Science and Technology(B)》 2011年第2期189-197,共9页
The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), (Coleoptera, Curculionidae, Rhynchophorinae), is an invasive species that is originated from Southeast Asia. It has invaded Middle East, several co... The Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Olivier), (Coleoptera, Curculionidae, Rhynchophorinae), is an invasive species that is originated from Southeast Asia. It has invaded Middle East, several countries of the Mediterranean Basin, Caribbean (Island of Curacao, Netherland Antilles), Lebanon and United States of America (Laguna Beach, Orange County, California) during the last three decades where it attacks palm trees. This study investigated the geographical variation among geographic populations of RPW collected from twelve invaded countries using cytochrome b (Cytb) and ITS2 partial sequences. The comparison among the Cytb sequences resulted in three different haplotypes designated as HB 1 to HB3. The three haplotypes were subdivided into two phylogenetic groups according to their geographic positions: (1) the "Multi-Continent" group containing HB 1 haplotype detected in eight populations belonging to three different Continents such as Africa-Egypt, Asia-Kingdom of Saudi Arabia and Turkey and Europe-Spain, Italy, Greece, Cyprus and France, and (2) the "Asian" group includes four populations from Iran (harboring HB2 haplotype), Pakistan, UAE, and Oman (harboring HB3 haplotype). This mitochondrial pattern of genetic diversity suggests that the tested RPW populations subdivided genetically into different sub-populations under the influence of genetic drift. According to these results we concluded that the twelve studied RPW populations are originating from different source populations in the RPW area of origin. 展开更多
关键词 Cytochrome b ITS2 Red Palm Weevil invasive species geographical variation.
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The Cytochrome b Polymorphism of Meat Lines Rabbits
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作者 Vladimir Parkanyi Lubomir Ondruska Dusan Vasicek 《Journal of Life Sciences》 2016年第1期16-20,共5页
The cytochrome b mtDNA was analysed from peripheral whole blood samples of meat lines rabbits. The PCR product--692 bp long fragment of Oryctolagus cuniculus_cytochrome b (0CU07566 GenBank, NCB1, USA) was amplified.... The cytochrome b mtDNA was analysed from peripheral whole blood samples of meat lines rabbits. The PCR product--692 bp long fragment of Oryctolagus cuniculus_cytochrome b (0CU07566 GenBank, NCB1, USA) was amplified. Synthetically produced oligonucleotides for the detection ofcyt b were designed by own algorithm: ORYCTO-cyt b-FOR 5'- CTA TCA GCA ATC CCA TAT ATC -3' and ORYCTO-cyt b-REV 5'- CTT CAT TTG AGG ATT TTG TT -3'. Based on AluI-RFLP were described two cytochrome b haplotypes--cyt b 430 and cyt b 306. Haplotype cyt b 430 is 571A (190Threonine-T) + 877G (292Alanine-A). The new haplotype cyt b 306 is presented A571G nucleotide substitution, i.e, 571G (190Alanine-A) + 877G (292Alanine-A). 展开更多
关键词 RAbbIT cytochrome b SNP new haplotype A571G.
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The Taxonomic Status of Gymnura bimaculata and G. japonica: Evidence from Mitochondrial DNA Sequences
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作者 Anglv Shen Chunyan Ma Yong Ni Zhaoli Xu Lingbo Ma 《Journal of Life Sciences》 2012年第1期9-13,共5页
Japanese butterfly ray Gymnura japonica from twinspot butterfly ray G. bimaculata based on a pair of white spots behind eyes or not, which was not reliable. To clarify the taxonomic status of G. japonica and G. bimacu... Japanese butterfly ray Gymnura japonica from twinspot butterfly ray G. bimaculata based on a pair of white spots behind eyes or not, which was not reliable. To clarify the taxonomic status of G. japonica and G. bimaculata, the nucleotide variation between the two butterfly rays was examined using mitochondrial DNA sequence comparisons. Approximately 585 bp of 16S ribosomal RNA (rRNA) and 1,128 bp cytochrome b (cyt b) genes were sequenced from representatives of two butterfly rays species in East China Sea. The results showed that there were the same sequences of 16S rRNA gene between two butterfly rays; six sites were variable among two butterfly rays of cyt b genes, the proportion of polymorphie loci was 0.53%, and two haplotypes were defined which genetic distance was 0.5%. Combined with the morphological character and the analysis of mtDNA sequence indicated that twinspot butterfly ray G. bimaculata was a synonym of Japanese butterfly ray G. japonica. 展开更多
关键词 16S rRNA cyt b morphological character taxonomic status butterfly ray.
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Molecular Evolution of the Cytochrome b Gene in Geographically Isolated Populations of Oryzias Latipes
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作者 Nobuhiko Asada Akira Hirai +3 位作者 Taiki Hamada Eri Sato Akio Moriyama Tetsuro Takeuchi 《Journal of Life Sciences》 2011年第4期282-286,共5页
The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3... The mitochondrial cytochrome b gene was isolated from the caudal fin of Oryzias latipes and PCR was undertaken to determine phylogeny. The nucleotide sequence of the complete cytochrome b gene including the 5' and 3' ends was 1,143 base pairs (bp) and 1,137 bp encoding 380 and 378 amino acids in the K-11 strain and HS strain, respectively. In addition, higher substitutions both in base and amino acid residues occurred more frequently between the former control (Hd-rR strain) and HS strains than the K-I 1 strain. Approximately similar values in polymorphism, as assessed by restriction endonuclease digestion, were detected when utilizing 20 different enzymes. Therefore, the evolutionary processes were unlikely to involve common ancestors, especially between the K-11 strain and HS strains, in O. latipes populations in Japan. The sequence had been deposited in GenBank Data Base under accession number AB480878 (K-I I strain) and AB480879 (HS strain). 展开更多
关键词 Cytochrome b RFLP DIVERSITY EVOLUTION Oryzias latipes.
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华南西部及海南岛美丽小条鳅种群遗传变异与亲缘地理 被引量:8
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作者 丘城锋 林岳光 +2 位作者 庆宁 赵俊 陈湘粦 《动物学报》 SCIE CAS CSCD 北大核心 2008年第5期805-813,共9页
通过分析108尾采自华南西部12条水系的美丽小条鳅(Micronemacheilus pulcher)mtDNA细胞色素b(cytb)基因全序列,研究其种群遗传变异和亲缘生物地理格局。美丽小条鳅的cytb序列包含138个核苷酸变异位点(占全序列12.11%)。分子变异分析(AMO... 通过分析108尾采自华南西部12条水系的美丽小条鳅(Micronemacheilus pulcher)mtDNA细胞色素b(cytb)基因全序列,研究其种群遗传变异和亲缘生物地理格局。美丽小条鳅的cytb序列包含138个核苷酸变异位点(占全序列12.11%)。分子变异分析(AMOVA)显示,分子遗传变异主要来自种群内(58.53%)和地理区内种群间(42.84%)。采用邻接法构建的39个单倍型的NJ系统树显示,12条水系的美丽小条鳅聚成两支。其中,广西沿海诸独立水系(防城河、峒中河、北仑河、南流江)和横穿广东和广西的西江水系与广东漠阳江和潭江水系关系密切;而海南岛万泉河和南渡江与广东鉴江水系关系密切。根据嵌套进化枝系地理分析(NCPA)推测,与越南毗邻的防城河周边地区可能是美丽小条鳅的扩散中心,该物种可由此区域通过两条途径扩散:1)沿西江水系向广西沿海独立水系至广东漠阳江和潭江水系扩散;2)向海南岛诸水系再至雷州半岛的鉴江水系扩散。在演化过程中,曾发生片断化事件、长距离建群和持续的分布区扩张。 展开更多
关键词 美丽小条鳅 细胞素色b基因 种群遗传 亲缘地理 华南西部 海南岛
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Mitochondrial DNA Sequences Imply Anhui Musk Deer a Valid Species in Genus Moschus 被引量:13
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作者 宿兵 王应祥 王岐山 《Zoological Research》 CAS CSCD 北大核心 2001年第3期169-173,共5页
Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b ge... Anhui musk deer [ Moschus ( moschiferus/berezovskii ) anhuiensis ] has been a taxonomic mystery since its discovery in early 80's.In this paper,with museum samples,we sequenced the complete cytochrome b gene of five Anhui musk deer.When compared with other species in Genus Moschus ,Anhui musk deer showed a rather level of sequence divergence from all the other species in this genus.The phylogenetic trees constructed by multiple methods supported the same topology,in which the monophyly of Anhui musk deer was clearly demonstrated.Therefore,our molecular data suggest a full species status for Anhui musk deer ( Moschus anhuiensis ),rather than a subspecies of either M moschiferus or M berezovskii previously suggested by morphological studies. 展开更多
关键词 Musk deer Genus Moschus Anhui musk deer Cytochrome b gene Molecular phylogeny
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Phylogeny and species differentiation of Mollitrichosiphum spp. (Aphididae, Greenideinae) based on mitochondrial CO1 and Cyt b genes 被引量:2
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作者 Ruiling ZHANG Xiaolei HUANG +1 位作者 Liyun JIANG Gexia QIAO 《Current Zoology》 SCIE CAS CSCD 北大核心 2011年第6期806-815,共10页
Phylogenetic analyses based on mitochondrial genes were conducted to reconstruct species relationships within the aphid genus MoUitrichosiphum (Aphididae, Greenideinae). MP and Bayesian analysis results using COI an... Phylogenetic analyses based on mitochondrial genes were conducted to reconstruct species relationships within the aphid genus MoUitrichosiphum (Aphididae, Greenideinae). MP and Bayesian analysis results using COI and Cyt b datasets, and combined MP, ML and Bayesian analysis of both were consistent with a morphologically supported monophyly. Subdivision of the genus into two subgenera was strongly supported. Samples of each included species form monophyletic clade, respectively; and the result implied the valid status of related species in this genus. These results suggest some surprising hypotheses regarding the phylogeography of the genus: the uplift of the Tibetan Plateau, reorganization of major fiver catchments and the isolation of Hainan Island were probably important factors contributing to the diversification of species in this genus . 展开更多
关键词 Mollitrichosiphum Geographical barrier PHYLOGENY Tibetan Plateau Hainan Island
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Cyprinid phylogeny based on Bayesian and maximum likelihood analyses of partitioned data:implications for Cyprinidae systematics 被引量:2
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作者 WANG XuZhen GAN XiaoNi +2 位作者 LI JunBing MAYDEN Richard L. HE ShunPing 《Science China(Life Sciences)》 SCIE CAS 2012年第9期761-773,共13页
Cyprinidae is the biggest family of freshwater fish, but the phylogenetic relationships among its higher-level taxa are not yet fully resolved. In this study, we used the nuclear recombination activating gene 2 and th... Cyprinidae is the biggest family of freshwater fish, but the phylogenetic relationships among its higher-level taxa are not yet fully resolved. In this study, we used the nuclear recombination activating gene 2 and the mitochondrial 16S ribosomal RNA and cytochrome b genes to reconstruct cyprinid phylogeny. Our aims were to (i) demonstrate the effects of partitioned phylo- genetic analyses on phylogeny reconstruction of cyprinid fishes; (ii) provide new insights into the phylogeny of cyprinids. Our study indicated that unpartitioned strategy was optimal for our analyses; partitioned analyses did not provide better-resolved or -supported estimates of cyprinid phylogeny. Bayesian analyses support the following relationships among the major mono- phyletic groups within Cyprinidae: (Cyprininae, Labeoninae), ((Acheilognathinae, ((Leuciscinae, Tincinae), Gobioninae)), Xenocyprirlinae). The placement of Danioninae was poorly resolved. Estimates of divergence dates within the family showed that radiation of the major cyprinid groups occurred during the Late Oligocene through the Late Miocene. Our phylogenetic analyses improved our understanding of the evolutionary history of this important fish family. 展开更多
关键词 CYPRINIDAE PHYLOGENY molecular dating partitioned analyses
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Are genetically distinct lizard species able to hybridize? A review 被引量:1
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作者 Jitka JANCUCHOVA-LASKOVA Eva LANDOVA Daniel FRYNTA 《Current Zoology》 SCIE CAS CSCD 2015年第1期155-180,共26页
Animal species are delimited by reproductive isolation mechanisms (RIMs). Postzygotic RIMs are mainly products of genetic differences and thus their strength increases with elapsed divergence time. The relationship ... Animal species are delimited by reproductive isolation mechanisms (RIMs). Postzygotic RIMs are mainly products of genetic differences and thus their strength increases with elapsed divergence time. The relationship between postzygotic repro- ductive isolation and genetic divergence, however, differs considerably among major clades of vertebrates. We reviewed the available literature providing empirical evidence of natural and/or experimental hybridization between distinct species of lizards (squamates except snakes). We found that hybridization events are widely distributed among nearly all major lizard clades. The majority of research focuses on parthenogenetic species and/or polyploid hybrids in families Lacertidae, Teiidae and Gekkonidae. Homoploid bisexual hybrids are mainly reported within Lacertidae and Iguania groups. As a proxy of genetic divergence of the hybridizing taxa we adopted nucleotide sequence distance (HKY85) of mitochondrial cyt b gene. The upper limit of genetic di- vergence was similar with regard to both parthenogenetic and bisexual hybrids. Maximum values of these distances between hy- bridizing species of lizards approached 18%-21%, which is comparable to or even exceeds the corresponding values reported for other principal clades of vertebrates. In spite of this, F1 hybrids are typically at least partially fertile in lizards and thus genetic introgression between highly divergent species is possible. The relationship between the genetic distance and hybrid fertility was not found [Current Zoology 61 (1): 155-180, 2015]. 展开更多
关键词 Hybridization INTROGRESSION FERTILITY VIAbILITY Genetic divergence LIZARDS
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Ancient DNA sequences from Coelodonta antiquitatis in China reveal its divergence and phylogeny 被引量:1
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作者 YUAN JunXia SHENG GuiLian +4 位作者 HOU XinDong SHUANG XiaoYan YI Jian YANG Hong LAI XuLong 《Science China Earth Sciences》 SCIE EI CAS 2014年第3期388-396,共9页
Ancient DNA data have supported a sister relationship between woolly rhinoceros and extant Sumatran rhinoceros.This relationship has been used to explore the divergent times for the woolly rhinoceros from their relati... Ancient DNA data have supported a sister relationship between woolly rhinoceros and extant Sumatran rhinoceros.This relationship has been used to explore the divergent times for the woolly rhinoceros from their relatives.Complete and partial ancient DNA sequences of the mitochondrial cytochrome b(cyt b)gene were retrieved from bones of the late Pleistocene Coelodonta antiquitatis excavated from northern and northeastern China.The newly obtained sequences together with the European and northern Asian Coelodonta antiquitatis sequences from GenBank were used to estimate the evolutionary divergence time.Phylogenetic analyses showed the exchange of genetic information between the Chinese individuals and Coelodonta antiquitatis of north Asia,which also indicated a more recent evolutionary timescale(3.8–4.7 Ma)than previous molecular estimations(17.5–22.8 or 21–26 Ma)for woolly rhinoceros based on the fossil calibration of outgroups.This new timescale was more consistent with the fossil record of the earliest known genus Coelodonta. 展开更多
关键词 Coelodonta antiquitatis cytochrome b gene divergence time PHYLOGENY
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