目的旨在建立南京地区不同年龄和性别表观健康成年人白细胞群落参数的参考区间。方法选择2024年1月至4月于南京大学医学院附属鼓楼医院进行体检的1262名健康成人作为研究对象。采用Sysmex XN全自动血细胞分析仪进行血常规检测,同时记录...目的旨在建立南京地区不同年龄和性别表观健康成年人白细胞群落参数的参考区间。方法选择2024年1月至4月于南京大学医学院附属鼓楼医院进行体检的1262名健康成人作为研究对象。采用Sysmex XN全自动血细胞分析仪进行血常规检测,同时记录仪器WDF通道提供的中性粒、淋巴和单核细胞相关的细胞群落参数(cell population data,CPD),统称为白细胞CPD,包括它们的平均侧向散射光强度(NE-SSC、LY-X、MO-X)和分布宽度(NE-WX、LY-WX、MO-WY);平均荧光强度(NE-SFL、LY-Y、MO-Y)和分布宽度(NE-WY、LY-WY、MO-WY);平均前向散射光强度(NE-FSC、LY-Z、MO-Z)和分布宽度(NE-WZ、LY-WZ、MO-WZ)。参照中华人民共和国卫生行业标准(WS/T405-2012)《血细胞分析参考区间》中的推荐方法,分析各项指标在不同年龄及性别间的差异,并得出南京健康成人白细胞CPD的参考区间。结果在男女两性别组内按年龄分组比较发现,LY-WZ、MO-WZ、NE-FCS、NE-WZ不显示年龄和性别上的差异;MO-Z只显示性别差异;LY-Y、LY-Z、MO-X、NE-SSC、NE-WY只在女性组内存在年龄差异;MO-WY、MO-Y、NE-SFL、NE-WX只在男性内存在年龄差异;LY-X、LY-WX、LY-WY、MO-WX在两性别组内均存在年龄差异。以此为依据划分参考区间。结论建立了南京地区白细胞CPD在不同性别和年龄段健康成年人中的参考区间,为疾病的诊断、治疗和预后判断提供重要的实验室参考依据。展开更多
目的探讨白细胞群落参数(leukocyte community parameters,CPD)在带状疱疹(herpes zoster,HZ)患者中的变化及临床应用。方法选取82例HZ患者并分组为HZ治疗前、HZ治疗后,健康对照者150例,采用Coulter DXH800全自动血液分析仪检测,包括中...目的探讨白细胞群落参数(leukocyte community parameters,CPD)在带状疱疹(herpes zoster,HZ)患者中的变化及临床应用。方法选取82例HZ患者并分组为HZ治疗前、HZ治疗后,健康对照者150例,采用Coulter DXH800全自动血液分析仪检测,包括中性粒细胞平均体积MNV、中性粒细胞平均体积分布宽度MNVSD、中性粒细胞平均传导率MNC、中性粒细胞平均传导率分布宽度MNCSD及淋巴细胞、单核细胞、嗜酸性粒细胞相关参数等共计16项指标。结果治疗前HZ患者的MNVSD水平显著低于健康对照组,MNC、MLV、MLVSD、MLC、MLCSD、MMV、MMVSD、MMC、MMCSD、MEOC、MEOCSD与健康人之间差异存在统计学意义;治疗后组与治疗前组比较,MLV、MLVSD、MMVSD、MMCSD治疗后组低于治疗前组,差异有统计学意义(P<0.01或P<0.05)。结论HZ患者CPD相对于健康对照人群有显著变化且治疗前后部分参数差异显著。展开更多
目的:探讨白细胞群落参数检测对抗结核药物致粒细胞下降诊断灵敏度以及特异性的影响。方法:选取我院51例抗结核药物致粒细胞下降患者作为观察组,另取50例同期健康体检者作为对照组,均行白细胞群落参数[中性粒细胞百分率(NE%)、中性粒细...目的:探讨白细胞群落参数检测对抗结核药物致粒细胞下降诊断灵敏度以及特异性的影响。方法:选取我院51例抗结核药物致粒细胞下降患者作为观察组,另取50例同期健康体检者作为对照组,均行白细胞群落参数[中性粒细胞百分率(NE%)、中性粒细胞绝对值(A N C)、中性粒细胞平均体积(MN V)、中性粒细胞平均光散射值(M NS)]检测,观察比较两组白细胞群落参数水平,并统计白细胞群落参数对抗结核药物致粒细胞下降的诊断灵敏度、特异性。结果观察组NE%、ANC及MNS均低于对照组,MNV高于对照组,差异具有统计学意义(P<0.05);白细胞群落各参数联合检测灵敏度为94.12(48/51)、特异度为94.00(47/50)、准确率为94.06(95/101),均高于NE%、ANC、MNV、MNS单一检测,误诊率为6.00(3/50)、漏诊率为5.88(3/51),均低于各单一检测,差异具有统计学意义(P<0.05)。结论:抗结核药物致粒细胞下降患者白细胞群落各参数水平均较健康者低,且各参数联合检测准确率、灵敏度及特异度高,误诊、漏诊率低。展开更多
目的研究细胞群落参数(CPD)在肺结核感染诊断中的应用价值。方法采用Dx H 800血液分析仪,分析239份全血样本,每个样本均获得56项白细胞CPD参数和56项有核红细胞CPD参数,其中观察组105例,对照组134例,比较观察组与对照组CPD参数间的差异...目的研究细胞群落参数(CPD)在肺结核感染诊断中的应用价值。方法采用Dx H 800血液分析仪,分析239份全血样本,每个样本均获得56项白细胞CPD参数和56项有核红细胞CPD参数,其中观察组105例,对照组134例,比较观察组与对照组CPD参数间的差异。结果2组间具有差异的CPD参数共有37项,其中5项CPD参数AUC>0.8,当MN-V-MO、SD-LALS-MO、MN-AL2-EO、SD-V-MO、SD-LMALS-N-GRMO截断值分别取≥167.5、≥25.81、≥117.5、≥18.14、≥33.66时,其灵敏度和特异度分别为72%和93%、88%和80%、75%和87%、84%和90%、63%和86%,将5种差异CPD参数建立诊断模型,MN-V-MO+SD-V-MO+SD-LALS-MO+MN-AL2-EO+SD-LMALS-N-GRMO联合诊断时,ROC曲线下面积为0.982,其灵敏度以及特异度分别达94%和99%。结论细胞CPD参数的检测可以协助临床诊断肺结核感染,具有较高的临床应用价值。展开更多
目的研究Uni Cel Dx H 800全自动血液分析仪(简称Dx H 800)白细胞(WBC)群落参数(CPD)对登革热临床诊断及鉴别诊断的价值,建立最佳报警阈值。方法选取登革热确诊患者278例、登革热疑似患者(临床症状与登革热相似,但血清学检测结果阴性)10...目的研究Uni Cel Dx H 800全自动血液分析仪(简称Dx H 800)白细胞(WBC)群落参数(CPD)对登革热临床诊断及鉴别诊断的价值,建立最佳报警阈值。方法选取登革热确诊患者278例、登革热疑似患者(临床症状与登革热相似,但血清学检测结果阴性)106例、健康体检者159名为研究对象。运用Dx H 800进行全血细胞计数和WBC分类计数。分析单核细胞和淋巴细胞CPD,利用受试者工作特征(ROC)曲线评价各参数及部分结果对登革热的诊断效率。结果登革热组患者WBC和血小板(PLT)计数相对于登革热疑似患者和健康体检者均显著降低(P<0.05),单核细胞百分比(MO%)显著增高(P<0.05),单核细胞大小显著不等,单核细胞体积的标准差(s-V-MO)显著增高(P<0.05)。将登革热患者MO%和s-V-MO 2个变化比较显著的参数综合分析,得到登革因子参数。登革因子能很好地鉴别诊断登革热,与健康体检者鉴别时,ROC曲线下面积(AUC)为0.99,当临界值为29.3时,敏感性为95.7%,特异性为98.7%。与登革热疑似患者鉴别时,AUC为0.824,当临界值为34.8时,敏感性为78.3%,特异性为75.2%。结论登革因子对登革热实验室诊断和鉴别诊断具有临床应用价值。展开更多
The seasonal variations in phytoplankton community structure were investigated for the Sanggou Bay （SGB） and the adjacent Ailian Bay （ALB） and Lidao Bay （LDB） in Shandong Peninsula,eastern China.The species comp...The seasonal variations in phytoplankton community structure were investigated for the Sanggou Bay （SGB） and the adjacent Ailian Bay （ALB） and Lidao Bay （LDB） in Shandong Peninsula,eastern China.The species composition and cell abundance of phytoplankton in the bay waters in spring （April 2011）,summer （August 2011）,autumn （October 2011）,and winter （January 2012） were examined using the Uterm6hl method.A total of 80 taxa of phytoplankton that belong to 39 genera of 3 phyla were identified.These included 64 species of 30 genera in the Phylum Bacillariophyta,13 species of 8 genera in the Phylum Dinophyta,and 3 species of 1 genus in the Phylum Chrysophyta.During the four seasons,the number of phytoplankton species （43） was the highest in spring,followed by summer and autumn （40）,and the lowest number ofphytoplankton species （35） was found in winter.Diatoms,especially Paralia sulcata （Ehrenberg） Cleve and Coscinodiscus oculus-iridis Ehrenberg,were predominant in the phytoplankton community throughout the study period,whereas the dominance of dinoflagellate appeared in summer only.The maximum cell abundance of phytoplankton was detected in summer （average 8.08 × 103 cells L-1） whereas their minimum abundance was found in autumn （average 2.60 x 103 cellsL-1）.The phytoplankton abundance was generally higher in the outer bay than in the inner bay in spring and autumn.In summer,the phytoplankton cells were mainly concentrated in the south of inner SGB,with peak abundance observed along the western coast.In winter,the distribution of phytoplankton cells showed 3 patches,with peak abundance along the western coast as well.On seasonal average,the Shannon-Wiener diversity indices of phytoplankton community ranged from 1.17 to 1.78 （autumn 〉 summer 〉 spring 〉 winter）,and the Pielou＇s evenness indices of phytoplankton ranged from 0.45 to 0.65 （autumn 〉 spring 〉 summer〉 winter）.According to the results of canonical correspondence analysis,phosphate level was the major factor that limited the occurrence of P.sulcata and C.oculus-iridis,whereas optimal temperature and low salinity were responsible for Prorocentrum blooms in summer.The detailed description of seasonal variations in phytoplankton community structure in the three bays provide reference data for future studies on marine ecosystems and mariculture in adjacent areas.展开更多
DNA barcoding provides accurate stages. Single-gene-targeted metagenomic analysis identification of zooplankton species through all life based on DNA barcode databases can facilitate long- term monitoring of zooplankt...DNA barcoding provides accurate stages. Single-gene-targeted metagenomic analysis identification of zooplankton species through all life based on DNA barcode databases can facilitate long- term monitoring of zooplankton communities. With the help of the available zooplankton databases, the zooplankton community of the Changjiang （Yangtze） River estuary was studied using a single-gene-targeted metagenomic method to estimate the species richness of this community. A total of 856 mitocbondrial cytochrome oxidase subunit 1 （coxl） gene sequences were determined. The environmental barcodes were clustered into 70 molecular operational taxonomic units （MOTUs）. Forty-two MOTUs matched barcoded marine organisms with more than 90% similarity and were assigned to either the species （similarity〉96%） or genus level （similarity〈96%）. Sibling species could also be distinguished. Many species that were overlooked by morphological methods were identified by molecular methods, especially gelatinous zooplankton and merozooplankton that were likely sampled at different life history phases. Zooplankton community structures differed significantly among all of the samples. The MOTU spatial distributions were influenced by the ecological habits of the corresponding species. In conclusion, single-gene-targeted metagenomic analysis is a useful tool for zooplankton studies, with which specimens from all life history stages can be identified quickly and effectively with a comprehensive database.展开更多
文摘目的旨在建立南京地区不同年龄和性别表观健康成年人白细胞群落参数的参考区间。方法选择2024年1月至4月于南京大学医学院附属鼓楼医院进行体检的1262名健康成人作为研究对象。采用Sysmex XN全自动血细胞分析仪进行血常规检测,同时记录仪器WDF通道提供的中性粒、淋巴和单核细胞相关的细胞群落参数(cell population data,CPD),统称为白细胞CPD,包括它们的平均侧向散射光强度(NE-SSC、LY-X、MO-X)和分布宽度(NE-WX、LY-WX、MO-WY);平均荧光强度(NE-SFL、LY-Y、MO-Y)和分布宽度(NE-WY、LY-WY、MO-WY);平均前向散射光强度(NE-FSC、LY-Z、MO-Z)和分布宽度(NE-WZ、LY-WZ、MO-WZ)。参照中华人民共和国卫生行业标准(WS/T405-2012)《血细胞分析参考区间》中的推荐方法,分析各项指标在不同年龄及性别间的差异,并得出南京健康成人白细胞CPD的参考区间。结果在男女两性别组内按年龄分组比较发现,LY-WZ、MO-WZ、NE-FCS、NE-WZ不显示年龄和性别上的差异;MO-Z只显示性别差异;LY-Y、LY-Z、MO-X、NE-SSC、NE-WY只在女性组内存在年龄差异;MO-WY、MO-Y、NE-SFL、NE-WX只在男性内存在年龄差异;LY-X、LY-WX、LY-WY、MO-WX在两性别组内均存在年龄差异。以此为依据划分参考区间。结论建立了南京地区白细胞CPD在不同性别和年龄段健康成年人中的参考区间,为疾病的诊断、治疗和预后判断提供重要的实验室参考依据。
文摘目的探讨白细胞群落参数(leukocyte community parameters,CPD)在带状疱疹(herpes zoster,HZ)患者中的变化及临床应用。方法选取82例HZ患者并分组为HZ治疗前、HZ治疗后,健康对照者150例,采用Coulter DXH800全自动血液分析仪检测,包括中性粒细胞平均体积MNV、中性粒细胞平均体积分布宽度MNVSD、中性粒细胞平均传导率MNC、中性粒细胞平均传导率分布宽度MNCSD及淋巴细胞、单核细胞、嗜酸性粒细胞相关参数等共计16项指标。结果治疗前HZ患者的MNVSD水平显著低于健康对照组,MNC、MLV、MLVSD、MLC、MLCSD、MMV、MMVSD、MMC、MMCSD、MEOC、MEOCSD与健康人之间差异存在统计学意义;治疗后组与治疗前组比较,MLV、MLVSD、MMVSD、MMCSD治疗后组低于治疗前组,差异有统计学意义(P<0.01或P<0.05)。结论HZ患者CPD相对于健康对照人群有显著变化且治疗前后部分参数差异显著。
文摘目的:探讨白细胞群落参数检测对抗结核药物致粒细胞下降诊断灵敏度以及特异性的影响。方法:选取我院51例抗结核药物致粒细胞下降患者作为观察组,另取50例同期健康体检者作为对照组,均行白细胞群落参数[中性粒细胞百分率(NE%)、中性粒细胞绝对值(A N C)、中性粒细胞平均体积(MN V)、中性粒细胞平均光散射值(M NS)]检测,观察比较两组白细胞群落参数水平,并统计白细胞群落参数对抗结核药物致粒细胞下降的诊断灵敏度、特异性。结果观察组NE%、ANC及MNS均低于对照组,MNV高于对照组,差异具有统计学意义(P<0.05);白细胞群落各参数联合检测灵敏度为94.12(48/51)、特异度为94.00(47/50)、准确率为94.06(95/101),均高于NE%、ANC、MNV、MNS单一检测,误诊率为6.00(3/50)、漏诊率为5.88(3/51),均低于各单一检测,差异具有统计学意义(P<0.05)。结论:抗结核药物致粒细胞下降患者白细胞群落各参数水平均较健康者低,且各参数联合检测准确率、灵敏度及特异度高,误诊、漏诊率低。
文摘目的研究细胞群落参数(CPD)在肺结核感染诊断中的应用价值。方法采用Dx H 800血液分析仪,分析239份全血样本,每个样本均获得56项白细胞CPD参数和56项有核红细胞CPD参数,其中观察组105例,对照组134例,比较观察组与对照组CPD参数间的差异。结果2组间具有差异的CPD参数共有37项,其中5项CPD参数AUC>0.8,当MN-V-MO、SD-LALS-MO、MN-AL2-EO、SD-V-MO、SD-LMALS-N-GRMO截断值分别取≥167.5、≥25.81、≥117.5、≥18.14、≥33.66时,其灵敏度和特异度分别为72%和93%、88%和80%、75%和87%、84%和90%、63%和86%,将5种差异CPD参数建立诊断模型,MN-V-MO+SD-V-MO+SD-LALS-MO+MN-AL2-EO+SD-LMALS-N-GRMO联合诊断时,ROC曲线下面积为0.982,其灵敏度以及特异度分别达94%和99%。结论细胞CPD参数的检测可以协助临床诊断肺结核感染,具有较高的临床应用价值。
文摘目的研究Uni Cel Dx H 800全自动血液分析仪(简称Dx H 800)白细胞(WBC)群落参数(CPD)对登革热临床诊断及鉴别诊断的价值,建立最佳报警阈值。方法选取登革热确诊患者278例、登革热疑似患者(临床症状与登革热相似,但血清学检测结果阴性)106例、健康体检者159名为研究对象。运用Dx H 800进行全血细胞计数和WBC分类计数。分析单核细胞和淋巴细胞CPD,利用受试者工作特征(ROC)曲线评价各参数及部分结果对登革热的诊断效率。结果登革热组患者WBC和血小板(PLT)计数相对于登革热疑似患者和健康体检者均显著降低(P<0.05),单核细胞百分比(MO%)显著增高(P<0.05),单核细胞大小显著不等,单核细胞体积的标准差(s-V-MO)显著增高(P<0.05)。将登革热患者MO%和s-V-MO 2个变化比较显著的参数综合分析,得到登革因子参数。登革因子能很好地鉴别诊断登革热,与健康体检者鉴别时,ROC曲线下面积(AUC)为0.99,当临界值为29.3时,敏感性为95.7%,特异性为98.7%。与登革热疑似患者鉴别时,AUC为0.824,当临界值为34.8时,敏感性为78.3%,特异性为75.2%。结论登革因子对登革热实验室诊断和鉴别诊断具有临床应用价值。
基金supported by the National Program on Key Basic Research Project of China (Grant Nos. 2011CB409804 and 2015CB954002)Program for New Century Excellent Talents in University (NCET-12-1065)the National Natural Science Foundation of China (Grant No.41176136) to J. Sun
文摘The seasonal variations in phytoplankton community structure were investigated for the Sanggou Bay （SGB） and the adjacent Ailian Bay （ALB） and Lidao Bay （LDB） in Shandong Peninsula,eastern China.The species composition and cell abundance of phytoplankton in the bay waters in spring （April 2011）,summer （August 2011）,autumn （October 2011）,and winter （January 2012） were examined using the Uterm6hl method.A total of 80 taxa of phytoplankton that belong to 39 genera of 3 phyla were identified.These included 64 species of 30 genera in the Phylum Bacillariophyta,13 species of 8 genera in the Phylum Dinophyta,and 3 species of 1 genus in the Phylum Chrysophyta.During the four seasons,the number of phytoplankton species （43） was the highest in spring,followed by summer and autumn （40）,and the lowest number ofphytoplankton species （35） was found in winter.Diatoms,especially Paralia sulcata （Ehrenberg） Cleve and Coscinodiscus oculus-iridis Ehrenberg,were predominant in the phytoplankton community throughout the study period,whereas the dominance of dinoflagellate appeared in summer only.The maximum cell abundance of phytoplankton was detected in summer （average 8.08 × 103 cells L-1） whereas their minimum abundance was found in autumn （average 2.60 x 103 cellsL-1）.The phytoplankton abundance was generally higher in the outer bay than in the inner bay in spring and autumn.In summer,the phytoplankton cells were mainly concentrated in the south of inner SGB,with peak abundance observed along the western coast.In winter,the distribution of phytoplankton cells showed 3 patches,with peak abundance along the western coast as well.On seasonal average,the Shannon-Wiener diversity indices of phytoplankton community ranged from 1.17 to 1.78 （autumn 〉 summer 〉 spring 〉 winter）,and the Pielou＇s evenness indices of phytoplankton ranged from 0.45 to 0.65 （autumn 〉 spring 〉 summer〉 winter）.According to the results of canonical correspondence analysis,phosphate level was the major factor that limited the occurrence of P.sulcata and C.oculus-iridis,whereas optimal temperature and low salinity were responsible for Prorocentrum blooms in summer.The detailed description of seasonal variations in phytoplankton community structure in the three bays provide reference data for future studies on marine ecosystems and mariculture in adjacent areas.
基金Supported by the National Natural Science Foundation of China(No.41230963)the National Basic Research Program of China(973 Program)(No.2011CB403604)+2 种基金the"135"Fund of Institute of Oceanology,Chinese Academy of Sciences(No.2012I0060102)the Innovative Research Group Funding of the National Natural Science Foundation of China(No.41121064)the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDA11020305)
文摘DNA barcoding provides accurate stages. Single-gene-targeted metagenomic analysis identification of zooplankton species through all life based on DNA barcode databases can facilitate long- term monitoring of zooplankton communities. With the help of the available zooplankton databases, the zooplankton community of the Changjiang （Yangtze） River estuary was studied using a single-gene-targeted metagenomic method to estimate the species richness of this community. A total of 856 mitocbondrial cytochrome oxidase subunit 1 （coxl） gene sequences were determined. The environmental barcodes were clustered into 70 molecular operational taxonomic units （MOTUs）. Forty-two MOTUs matched barcoded marine organisms with more than 90% similarity and were assigned to either the species （similarity〉96%） or genus level （similarity〈96%）. Sibling species could also be distinguished. Many species that were overlooked by morphological methods were identified by molecular methods, especially gelatinous zooplankton and merozooplankton that were likely sampled at different life history phases. Zooplankton community structures differed significantly among all of the samples. The MOTU spatial distributions were influenced by the ecological habits of the corresponding species. In conclusion, single-gene-targeted metagenomic analysis is a useful tool for zooplankton studies, with which specimens from all life history stages can be identified quickly and effectively with a comprehensive database.
