AIM： To investigate cytokine production and cell surface phenotypes of dendritic cells （DC） in the presence of epithelial cells stimulated by probiotics.METHODS： Mouse DC were cultured alone or together with mouse...AIM： To investigate cytokine production and cell surface phenotypes of dendritic cells （DC） in the presence of epithelial cells stimulated by probiotics.METHODS： Mouse DC were cultured alone or together with mouse epithelial cell monolayers in normal or in- verted systems and were stimulated with heat-killed probiotic bacteria, Bifidobacterium lactis ADO 11 （BL）, Bifidobacterium bilfidum BGN4 （BB）, Lactobacillus casei IBS041 （LC）, and Lactobacillus acidophilus AD031 （LA）, for 12 h. Cytokine levels in the culture supernatants were determined by enzyme-linked immunosorbent as say and phenotypic analysis of DC was investigated by flow cytometry.RESULTS： BB and LC in singlecultured DC increased the expression of I-Ad, CD86 and CD40 （I-Ad, 18.51 vs 30.88, 46.11, CD86, 62.74 vs 92.7, 104.12; CD40, 0.67 vs 6.39, 3.37, P 〈 0.05）. All of the experimental probiot-ics increased the production of inflammatory cytokines, interleukin （IL）-6 and tumor necrosis factor （TNF）-α. However, in the normal coculture systems, LC and LA decreased the expression of I-A^α （39.46 vs 30.32, 33.26, P 〈 0.05）, and none of the experimental probiotics increased the levels of IL-6 or TNF-α. In the inverted coculture systems, LC decreased the expression of CD40 （1.36 vs -2.27, P 〈 0.05）, and all of the experimental probiotics decreased the levels of IL-6. In addition, BL increased the production of IL-10 （103.8 vs 166.0, P 〈 0.05） and LC and LA increased transforming growth factor-13 secretion （235.9 vs 618.9, 607.6, P 〈 0.05）.CONCLUSION： These results suggest that specific pro- biotic strains exert differential immune modulation mediated by the interaction of dendritic cells and epithelial cells in the homeostasis of gastrointestinal tract.展开更多
趋化因子(chemoattractant cytokine ligand,CCL)是一类可趋化细胞移动的小分子肽物质,根据其分子结构中N-末端半胱氨酸的相对位置主要分为四种类型,CCL17是其中CC型的趋化因子,也被称作胸腺和活化调节趋化因子(thymus and activation r...趋化因子(chemoattractant cytokine ligand,CCL)是一类可趋化细胞移动的小分子肽物质,根据其分子结构中N-末端半胱氨酸的相对位置主要分为四种类型,CCL17是其中CC型的趋化因子,也被称作胸腺和活化调节趋化因子(thymus and activation regulated chemokine,TARC).趋化因子通过结合细胞表面的趋化因子受体(chemokine receptor,CCR)发挥其生物学功能,CCL17对应的膜受体为CCR4分子,主要表达于多种T淋巴细胞亚群的细胞膜表面.研究发现CCL17具有多样的生物学功能,它能够趋化CCR4阳性的细胞发生迁移作用,参与到机体的炎症、超敏反应及自身免疫性疾病等多种病理生理过程,近年来有关CCL17参与肿瘤微环境及影响机体肿瘤免疫功能的研究多有报道,研究发现CCL17可能通过调节性T细胞(regulatory T cells,Treg)发挥促进肿瘤生长与转移的作用,但也有相关报道证实CCL17在特定条件下亦具有抗肿瘤免疫作用.展开更多
基金Supported by The Small and Medium Business Administration,No. S1072365the Next-Generation BioGreen 21 Program,No. PJ008005,Rural Development Administration,South Korea
文摘AIM： To investigate cytokine production and cell surface phenotypes of dendritic cells （DC） in the presence of epithelial cells stimulated by probiotics.METHODS： Mouse DC were cultured alone or together with mouse epithelial cell monolayers in normal or in- verted systems and were stimulated with heat-killed probiotic bacteria, Bifidobacterium lactis ADO 11 （BL）, Bifidobacterium bilfidum BGN4 （BB）, Lactobacillus casei IBS041 （LC）, and Lactobacillus acidophilus AD031 （LA）, for 12 h. Cytokine levels in the culture supernatants were determined by enzyme-linked immunosorbent as say and phenotypic analysis of DC was investigated by flow cytometry.RESULTS： BB and LC in singlecultured DC increased the expression of I-Ad, CD86 and CD40 （I-Ad, 18.51 vs 30.88, 46.11, CD86, 62.74 vs 92.7, 104.12; CD40, 0.67 vs 6.39, 3.37, P 〈 0.05）. All of the experimental probiot-ics increased the production of inflammatory cytokines, interleukin （IL）-6 and tumor necrosis factor （TNF）-α. However, in the normal coculture systems, LC and LA decreased the expression of I-A^α （39.46 vs 30.32, 33.26, P 〈 0.05）, and none of the experimental probiotics increased the levels of IL-6 or TNF-α. In the inverted coculture systems, LC decreased the expression of CD40 （1.36 vs -2.27, P 〈 0.05）, and all of the experimental probiotics decreased the levels of IL-6. In addition, BL increased the production of IL-10 （103.8 vs 166.0, P 〈 0.05） and LC and LA increased transforming growth factor-13 secretion （235.9 vs 618.9, 607.6, P 〈 0.05）.CONCLUSION： These results suggest that specific pro- biotic strains exert differential immune modulation mediated by the interaction of dendritic cells and epithelial cells in the homeostasis of gastrointestinal tract.
文摘趋化因子(chemoattractant cytokine ligand,CCL)是一类可趋化细胞移动的小分子肽物质,根据其分子结构中N-末端半胱氨酸的相对位置主要分为四种类型,CCL17是其中CC型的趋化因子,也被称作胸腺和活化调节趋化因子(thymus and activation regulated chemokine,TARC).趋化因子通过结合细胞表面的趋化因子受体(chemokine receptor,CCR)发挥其生物学功能,CCL17对应的膜受体为CCR4分子,主要表达于多种T淋巴细胞亚群的细胞膜表面.研究发现CCL17具有多样的生物学功能,它能够趋化CCR4阳性的细胞发生迁移作用,参与到机体的炎症、超敏反应及自身免疫性疾病等多种病理生理过程,近年来有关CCL17参与肿瘤微环境及影响机体肿瘤免疫功能的研究多有报道,研究发现CCL17可能通过调节性T细胞(regulatory T cells,Treg)发挥促进肿瘤生长与转移的作用,但也有相关报道证实CCL17在特定条件下亦具有抗肿瘤免疫作用.
