甘蓝型油菜波里马雄性不育的感温性

选用２１个不同育性水平的波里马细胞质雄性不育杂交组合和２个普通品种，对花器官形态和花粉量及活性进行观察鉴定，结果表明：１）试验涉及的波里马质不育组合为高温不育类型，其温度的影响还表现在雄蕊长度、♂／♀比值和花型大小。中间型材料温敏性较强，育性正常材料和１级高不育组合相对较弱。育性水平较稳定；２）在大田试验条件下，可根据花型、雄蕊长度和♂／♀来判断和选择不育性。一般盛花后，♂／♀＜０．４０，花瓣开放，肉眼未见花粉的组合可考虑选择利用；３）１级不育系雄蕊短、花粉量微、自交结实率极低且结实不正常，但开放授粉下，结实率和结实性均趋于正常，是目前较为理想的不育类型。

AFLP Analysis of Cytoplasmic Male-sterile Line and Its Maintainer Line of Pepper

[Objective] The aim of this study was to analyze the cytoplasmic male sterile line 21A and its maintainer line 21B of peppers by AFLP,and lay the foundation for further studies on molecular mechanism of the cytoplasmic male sterility in peppers.[Method] Cytoplasmic male sterility(CMS)line 21A and its maintainer line 21B were analyzed by AFLP to obtain the specific amplified fragments of cytoplasmic male sterile line 21A,while the specific amplified fragments were recovered or sequenced,and analyzed by BLAST...

Investigation on the Sterility Changeover of Male Sterility Line CMS7311 in Heading Chinese Cabbage

Sterility changeover induced by low temperature in male sterility line CMS7311 of heading Chinese cabbage ( Brassica campestris L. ssp. penkinsis) was investigated under constant temperature, day/night alternating temperature in light-growth-incubator and outdoor natural day/night alternating temperature respectively. It showed that the sterility of CMS7311 has significant change with temperature. The constant temperature of 6 - 12 degreesC was suitable to induce the changeover of sterility (CGS) of CMS7311 ; the shortest treatment time for CGS induction at this temperature was 3 d. The significant value of sterility changeover of CMS7311 occurred at the constant temperature of 6 - 9 degreesC after treatment for 6 - 9 d. The intensity of CGS was stronger under constant temperature than under alternate temperature, but the shortest treatment time inducing CGS has no difference between them. When treated for 9 d under the day/night alternating temperature of 11/7 degreesC, its sterility could also turn to normal fertility (near to sterile grade I or zero). In normal condition, the interval from the end of temperature treatment to the beginning of CGS induction was 10 - 16 d. In the condition of natural day/night alternating temperature, the lower limit of temperature for effective CGS induction was at the daily mean temperature ( DMT) of 3. 5 - 6. 8 degreesC, and the shortest treatment time which could induce the sterility changeover was also longer than that under constant temperature, being 11d at DMT of 1.5 - 3.1 degreesC. In addition, for the apical dominance, the flower bud on the first lateral branch showed the CGS 8 - 10 d later than the flower bud on the main stem under the same temperature with the same days of treatment. The intensity of CGS of CMS7311 on the first lateral branch was also weaker than the flower bud on the main stem.

Transcriptional Regulation of 10 Mitochondrial Genes in Different Tissues of NCa CMS System in Brassica napus L. and Their Relationship with Sterility

Northern blot analysis was conducted with mitochondrial RNA from seedling leaves, floral buds, and developing seeds of NCa CMS, maintainer line and fertile F1 using ten mitochondrial genes as probes. The results revealed that 9 out of the 10 mitochondrial genes, except for atp6, showed no difference in different tissues of the corresponding materials of NCα CMS system and that they might be constitutively expressed genes. Eight genes, such as orf139, orf222, atpl, cox1, cox2, cob, rm5S, and rm26S, showed no difference among the three tissues of all the materials detected. So the expression of these eight genes was not regulated by nuclear genes and was not tissue-specific. The transcripts of atp9 were identical among different tissues, but diverse among different materials, indicating that transcription of atp9 was neither controlled by nuclear gene nor tissue-specific. Gene atp6 displayed similar transcripts with the same size among different tissues of all the materials but differed in abundance among tissues of corresponding materials and its expression might be tissue-specific under regulation of nuclear gene. Moreover, three transcripts of orf222 were detected in the floral buds of NCa cms and fertile F1, but no transcript was detected in floral buds of the maintainer line.The transcription of orf139 was similar to that of orf222 but only two transcripts of 0.8 kb and 0.6 kb were produced. The atp9 probe detected a single transcript of 0.6 kb in NCa cms and in maintainer line and an additional transcript of 1.2 kb in fertile F1. The relationship of expression of orf222, orf139, and atp9 with NCa sterility was discussed.

Primary Identification of Alien Chromatin in T911289,a Maintainer of Wheat Male Sterile Line with Cytoplasm of Aegilops kotschyi

The genomic composition of 1911289, a wheat ( Tritium aestivum L.) maintainer of K-CMS, was examined by several methods, such as genomic in situ hybridization (GISH), biochemical marking, and DNA molecular marking. The results got by GISH and PCR amplification of dispersed rye-specific repetitive DNA sequence suggested that the alien chromatin in T911289 derived from rye. Specifically PCR amplification of the rye-specific microsatellite primers (SCM9) and seed storage protein analysis indicated that the alien chromatin in T911289 had developed from the short arm of 1R chromosome of rye (1RS). PCR amplification by using microsatellite primers locating on 1BS and seed storage protein analysis also revealed that 1911289 had lost the arm of 1BS or a small distal segment of it. We conclude that T911289 is a heterogeneous population which displays two distinct different types of translocation, i.e. the Robertsonian translocation and small segment translocation. The Robertsonian translocation type observed in our study is different from the 1BL/1RS translocation which is widely used in wheat production; it may be a novel and complex translocation form. Though the linkage between the desirable agronomic traits and the deleterious genes expressed as sticky dough has not got broken in T911289, the recovery of small segment translocation will still benefit the genetic study of wheat and rye.

Cytological Study on Zidao Type Cytoplasmic Male Sterile Line-Yingxiang A

Anthers of male sterile line and its maintainer line of Zidao type rice, Yingxiang A and Yingxiang B were studied by cytological methods, such as F-KI staining, silver nitrate staining and paraffin sections, It is found that in Yingxiang A, there are some abnormalities in meiosis, represented by unequal division at the meiotic phase; the abortion of CMS line arises at the meiotic phase and is almost completed at the phase of monomicrospore, but only a tiny amount of aborted mi- crospores are observed; the abortion is complete and thorough, and none normal functional pollens are found; the morphology of aborted pollens is similar to that of typically aborted pollens; the tapetal cells still exist in anthers from the microsporocyte stage to the tetrad stage, but they are abnormally hyperplastic. However, in Yingxiang B, there are no abnormalities in meiosis at the meiotic phase; the tapetal cells exist in anthers before the tetrad stage, but they disappear completely when the pollen are mature. It is indicated that the abortion of CMS Yingxiang line mainly occurs at the meiotic phase, and is basically completed at the phase of monomicrospore. The abortion is probably caused by the abnormally hyperplastic tapetal cells. The abnormalities in tapetal cells may affect the normal development of pollens, resulting in the emergence of abortion. In addition, the abortion in Yingxiang A is complete and thorough, which is of great significance in hybrid breeding.

Analysis of Heterosis, Combining Ability and Heritability of Cadmium Content in Brown Rice of Three-line Indica Hybrid Rice

Five cytoplasmic male sterile （CMS） lines were used as parents in an incomplete diallet cross and six restorer lines of rice design （Nc II design）. Thirty hybrid combinations with the same growth period were selected as experimental ma- terials to investigate the heterosis, combined ability and heredity of Cd content in brown rice of indica hybrid rice. According to the results, Cd content in brown rice showed a significantly negative heterosis; the general combining ability and specific combination ability of Cd content in CMS and restorer lines both reached extremely significant level （P〈0.01）, indicating that both genetic improvement of parents and e- valuation of combinations are important to the breeding of hybrid combinations with low accumulation of Cd; the broad-sense heritabitity and narrow-sense heritability of Cd content were both relatively high with slight differences, which respectively reached 97.73% and 80.10%, indicating that Cd content in brown rice mainly de- pends on the additive action of genes; in addition, parent improvement showed bet- ter effect on the selection of early generation.

Progress and future development of hybrid rapeseed in China

The hybrid growth of rapeseed now has reached to 4.7×106hm2,accounting for 70 % of total rapeseed growth area in China. Over the last 40 years,the researches and utilizations of hybrid rapeseed in China have significantly contributed to the development of rapeseed industry in China. The production of hybrid rapeseed mainly utilizes cytoplasmic male sterility(CMS),combined at the same time with nuclear male sterility and other pollinating-control systems in China. The hybrid rapeseed studies in China are also characterized by the improvement of quality and oil content in hybrid breeding. Future studies to enhance the heterosis of rapeseed will be focused on several important issues,including the combination of heterosis and ideotype breeding,further increase of oil content in hybrids,utilization of sub-genomic heterosis and resistance improvement. The paper will discuss the following perspectives in hybrid rapeseed studies:relationships among heterosis,quality and disease traits,solutions for excessive source and pool in hybrids compared with open-pollinated cultivars,and the importance of increasing harvest index of hybrids to achieve a better yield in hybrids.

Genetic Improvement and Biological Characteristic Analysis of Dwarf Abortive TypeJaponicaCMS Lines with Wide Compatibility

From the view of methodology for genetic improvement ofJaponicacytoplasmic male sterile(CMS) lines,we introduced the breeding process of the dwarf-abortive type(DA type)JaponicaCMS line Chunjiang 95A with wide compatibility,and further investigated the biological characteristics of the DA type Chunjiang 95A and its isonuclear alloplasmic CMS line Dian-I type Chunjiang 95A.The results show that DA type Chunjiang 95A is a japonicalinous CMS line and mainly typical abortive,which is better in fertility stability than stained abortive typeJaponica CMS lines.The DA type Chunjiang 95A is featured with high stigma exsertion,good flowering habit,high outcrossing rate and wide compatibility.Its recoverability differs from stained abortive typeJaponicaCMS lines.Moreover,we discussed genetic improvement of the typical abortive type JaponicaCMS lines and the application prospects of heterosis.

Confectionery Hybrid Sunflower Breeding in Russia

Confectionery sunflower hybrid breeding program started at VNIIMK in 1999. Aim of our study was to evaluate sunflower lines and hybrids, developed in this program. Best restorer lines were crossed with two lines with cytoplasmic male sterility （CMS-lines） used as testers. Hybrids were tested for seed yield, husk content and 1,000 seeds weight. Two-year hybrid trial allows us to define the most prominent hybrid combination （VK-905 A ~ K-4） and parental lines （CMS-line VK-905 A and restorer lines K-4, K-3 and K-I） for the future sunflower breeding work. It was further showed that confectionery hybrids with CMS-line VK-905 A do not show decrease in seed size with the increase in plant density.

Applicability of Cytoplasmic Male Sterility （CMS） as a Reliable Biological Confinement Method for the Cultivation of Genetically Modified Maize in Germany

The cultivation of genetically modified （GM） plants requires the reduction of an unwanted spread of genes （biological confinement）. Cytoplasmic male sterility （CMS） inhibits the development of functional pollen, but nuclear restorer （RJ） genes and environmental impacts can restore the fertility. The aim of this study was to verify whether CMS in maize hybrids is a reliable confinement method for the prospective cultivation of GM maize in Germany. Two-year field experiments in three different environments were conducted with three CMS maize hybrids which vary in the CMS stability, one conventional maize variety （all yellow kernels） and white maize as pollen recipient. Tassel characteristics, pollen vitality and cross-pollination rates were investigated. The CMS stability was dependent on the genotype and the specific weather conditions per year and location. In all maize hybrids CMS was unstable. One CMS maize hybrid showed a high level of CMS stability and very low cross-pollination rates in any case （〈 1%）. The two other CMS maize hybrids developed more fluctuant and fertile tassels with few or many pollen, respectively. Compared with a conventional and fully fertile maize variety, cross-pollination of all CMS maize hybrids was strongly reduced （84%-99%）. In conclusion, the CMS trait can be proposed as a useful biological confinement method to reduce pollen-mediated gene flow from GM maize.

Analysis of Fertility Differences of Peiai 64S in Hunan and Hainan

[Objective] The purpose was to study and analyze the difference of Peiai 64 S fertility expression in two different climatic regions, Hainan and Hunan in 2010, and confirm the suitable region for its production of hybrid seeds. [Method] Eight sowing times were designed, March 20 and 30, April 10 and 30, May 15, June 1, 10 and 20, respectively in Changsha of Hunan. Thirteen sowing times were designed in Hainan, January 28, February 12 and 27, March 14 and 29, April 13 and 28, May 13 and 28, June 12, July 1, 12 and 27, respectively. Peiai 64S was gradually managed and recorded heading stage during cultivation, and the effects of different climate influencing factors, such as daily mean temperature, daily maximum temperature, daily minimum temperature, duration of day at 0-25 d before heading （namely pollen mother cell meiophase）, on pollen fertility were analyzed to confirm sensitive periods of light and temperature. [ResuLt] There was a big difference in sensitive period among the same cytoplasmic male sterile （CMS） in different climatic regions. The sensitive period of Peiai 64S was in first four phases, phases V, VI, VII, VIII （0-13 d） in Changsha of Hunan, which was in last four phases, phases I, II, III, IV （13-25 d） in Haikou of Hainan. Under natural conditions, Peiai 64S pollen sterility rate were all more than 99.5% and seed-setting rates all less than 0.5% in Haikou, so production of hybrid seeds was safe. Its pollen sterility rate was 21.3%- 100.0% in Hunan, so its security coefficient of hybrid seeds production was lower than that in Hainan. [Conclusion] The security coefficient of hybrid seeds production of CMS Peiai 64S is lower than that in Hainan.

Design of Cellular Manufacturing Systems Considering Dynamic Production Planning and Worker Assignments

This article presents a comprehensive mathematical model for the design and analysis of Dynamic Cellular Manufacturing Systems （DCMS）. The proposed DCMS model considers several manufacturing attributes such as multi period production planning, dynamic system reconfiguration, duplicate machines, machine capacity, the available time for workers, worker assignments, and machine procurement. The objective is to minimize total costs; consisting of holding cost, outsourcing cost, inter-cell material handling cost, maintenance and overhead cost, machine relocation cost. While a study of published articles in the area of Cellular Manufacturing Systems （CMS） shows that workforce management issues have not sufficiently been addressed in the literature, the model presented also incorporates CMS workforce management issues such as salaries, hiring and firing costs of workers in addition to the manufacturing attributes. In-depth discussions on the results for two numerical examples are presented to illustrate applications of the proposed model. The model developed aims to raise the envelope by expanding and improving several CMS models previously presented in the literature.

TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in Brassica juncea

The T gene, which was cloned from the mitochondria of tumorous stem mustard （Brassica juncea var. tumida）, is a cytoplas- mic male sterility （CMS）-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the un- cleaved intron Tinll. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript （OE-T1243） showed a severe male-sterile phenotype, whereas OE-Tll70 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-Tll70 plants were unchanged. To investigate the potential roles of TinH, we inserted the TinH sequence upstream of a minimal region （-60） of the cauliflower mosaic virus 35S promoter fused to the 5＇ end of the 13-glucuronidase （GUS） reporter gene. Analysis of the transgenic plants suggested that TinH acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinH intron acts as an enhancer to affect the function of the CMS-related gene T.