用逐步回归方法探讨与鼻咽癌细胞间变有关的因素

本文用逐步回归的方法对１５项可能与鼻咽癌细胞间变有关的因素进行筛选。在Ｆ值为３时获得较优的回归方程，由该方程揭示了５项与癌细胞间变有关的因素。这些因素是癌间质中血管密度，癌生长范围，癌转移，癌ＷＨＯ分型和癌巢内淋巴细胞浸润量。癌细胞间变与前４项是正相关，说明间变癌细胞形态幼稚，生长活跃，易于浸润与转移。而癌巢内淋巴细胞浸润量与细胞间变是反相关，说明淋巴细胞浸润作为机体局部免疫反应，阻止癌细胞发展与间变。又因间变与患者预后密切相关，因此应将估价癌细胞间变的指标充实鼻咽癌的组织学分型中，使组织学类型具有预后意义。

血管外皮细胞瘤组织病理与预后分析

目的:探讨血管外皮细胞瘤的病理组织学特征与肿瘤预后的关系。方法:35例血管外皮细胞瘤以5年随访结果为标准分成良、恶性2组,其中良性2 0例,恶性15例,对这些肿瘤的病理组织学特征与随访结果作对比研究。结果:肿瘤直径和出血坏死机率在良恶性组间的差异有极显著性(P<0 .0 1) ,且细胞间变明显,网状纤维增多和部位深在的肿瘤多为恶性。结论:血管外皮细胞瘤的预后与肿瘤生长部位、大小、出血坏死、细胞间变及网状纤维的量有关,血管外皮细胞瘤是一种潜在恶性的肿瘤,远期转移复发为其特点。

T_2权重值与星形、少枝-星形及间变少枝-星形细胞瘤病理分型相关性研究

目的通过T2WI测量T2权重值与星形细胞瘤(AG)、少枝-星形细胞瘤(OA)及间变少枝-星形细胞瘤(A-OA)病理分型相关性。方法回顾性分析经手术病理证实的AG患者57例(男29例,女28例),OA 55例(男36例,女19例),A-OA 15例(男10例,女5例)。测量T2权重像上病灶中最高信号区与最低信号区,健侧皮层及深部白质信号。分别计算比值R(R1=最高信号区/皮层,R2=最低信号区/皮层,R3=最高信号区/深部白质,R4=最低信号区/深部白质)。统计分析不同分型的肿瘤之间R1、R3的差异;采用两两比较OA、AG与A-OA之间R3值的统计学差异。结果 R1及R3在不同分型的肿瘤之间差异有显著统计学意义,OA的R1、R3高于单纯的AG与A-OA。LSD两两比较AG与A-OA之间R3值差异无统计学意义,其余各组两两比较差异均有统计学意义。结论T2权重值与AG、OA及A-OA病理分型相关,OA肿块部分区域含水量高于单纯的AG与A-OA。

恶性胶质瘤组织中Bcl-xL mRNA和蛋白的表达及其与临床病理的关系

目的探讨恶性胶质瘤组织中bcl-xLmRNA和蛋白的表达及其与临床组织病理的关系。方法用免疫组化染色SP法和RT-PCR分别检测44例间变星形细胞瘤、45例胶质母细胞瘤和26例Ⅰ级星形细胞瘤组织中bcl-xLmRNA和蛋白的表达。结果bcl-xL蛋白表达的阳性率在间变星形细胞瘤中为86.40%,胶质母细胞瘤中为91.1%,与Ⅰ级星形细胞瘤的38.5%相比,差异显著(P<0.01)。bcl-xLmRNA相对含量在间变星形细胞瘤和胶质母细胞瘤中分别为0.89±0.26和0.93±0.21,与Ⅰ级星形细胞瘤的0.63±0.23相比,差异显著(P<0.01)。结论bcl-xLmRNA和蛋白表达与胶质瘤临床病理分级呈显著正相关,其高表达可能与胶质瘤的恶性进展密切相关。

52例尖锐湿疣的临床及冷冻治疗观察

尖锐湿疣(Condylomate acumiata简称CA)属性传播疾病之一。近年该病的发病率似有逐年增加趋势,现将我室1986年～1988年门诊诊治的52例报告如下,以期引起同行重视。

主动脉弓旁巨大化学感受器瘤1例

患者女,35岁。主诉有胸闷,咳嗽近两月。痰少,无血丝,不发热,时有心慌。经对症治疗不见好转。胸透诊为“左胸腔肿瘤。”

妇科

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Tidal effects on diel variations of picoplankton and viruses in the Changjiang estuary

Spatial distributions and seasonal variations of picoplankton (i.e. Synechococcus spp., Prochlorococcus spp., picoeukaryotes and heterotrophic bacteria) and viruses in the Changjiang estuary have been reported in the past. However, short-term variations (e.g. at a tidal timescale) of these organisms and their regulating factors remain unclear. We determined the time-series of fluctuations of picoplankton and viruses with tide simultaneously in flow cytometry in the Changjiang estuary during a cruise in June 2006, in which a tidal model based rectangle equation was applied. The results indicate that high cell abundances of picoplankton and viruses occurred during flood tide and low cell abundances during ebb tide. The period of the surface cell abundance variations was about 13 h, suggesting the surface cell abundances in the Changjiang estuary were largely regulated by tide. However, cell abundances in middle and bottom waters varied in different periods due to influences of tidal induced physical forces such as resuspension and stratification. Therefore, tidal action is an important factor for the diel variations of picoplankton and viruses in the Changjiang estuary.

cytoplasmic domain of tissue factor promotes liver fibrosis in mice

To evaluate the role of tissue factor (TF) and protease activated receptor (PAR)-2 in liver fibrosis. METHODSUsing CCl4 administration for eight weeks, we induced hepatic fibrosis in wild-type C57BL/6 mice and in mice with deletion of the cytoplasmic signalling domain of TF (TF§CT/§CT), deletion of PAR-2 (PAR-2-/-) and combined deletion of TF signalling domain and PAR-2 (TF§CT/§CT/PAR-2-/-). Hepatic fibrosis area was assessed by quantitative imaging of picrosirius red staining. Hepatic collagen content was assessed by hydroxyproline levels. Hepatic stellate cells (αSMA positive) and hepatic macrophages (CD68 positive) were identified by immunohistochemistry. Hepatic gene expression was determined by PCR and liver TGFβ1 content by ELISA. RESULTSCCl4 treated mice with deletion of the PAR-2 gene (PAR-2-/-) and the cytoplasmic domain of TF (TF§CT/§CT) developed significantly less hepatic fibrosis, characterised by reduced liver fibrosis area and hydroxyproline content, compared to control wildtype mice treated with CCl4. The observed reduction in histological fibrosis was accompanied by a significant decrease in the hepatic content of TGFβ, the prototypic fibrogenic cytokine, as well as fewer activated hepatic stellate cells and hepatic macrophages. Deletion of the TF cytoplasmic signalling domain reduced hepatic fibrosis to levels similar to those observed in mice lacking PAR-2 signalling but combined deletion provided no added protection against fibrosis indicating a lack of mutual modulating effects that have been observed in other contexts such as angiogenic responses. CONCLUSIONTissue factor cytoplasmic domain is involved in TF-PAR-2 signalling initiating hepatic fibrosis and is a potential therapeutic target, as its deletion would not impact coagulation.

Relationship between epithelial to mesenchymaltransition and chemoresistance of lung cancer

Objective： The aim of this study was to explore the correlation between epithelial to mesenchymal transition （EMT） and chemoresistance of non-small-cell lung cancer （NSCLC）. Methods： In vitro, the drug resistance index of cisplatin resistant lung adenocarcinoma cell line （A549/DDP） was detected by CCK-8 assay; the morphological change between A549/ DDP cells and lung adenocarcinoma cells （A549） was observed by phase contrast microscope; expression of EMT markers （including E-cadherin and vimentin） and resistance protein, excision repair cross-complementing 1 （ERCC1） was detected by immunocytochemistry. The expression of E-cadherin, vimentin and ERCC1 was investigated by immunohistochemistry in 120 cases of NSCLC, half of that were treated with pre-operative neoadjuvant chemotherapy （neoadjuvant chemotherapy group）, and the other underwent surgery alone （simple surgery group）. Results： There was a significant difference between the ICso （half maximal inhibitory concentration） of A549/DDP cells （5.20） and A549 cells （1.88） （P 〈 0.05）, and the drug resistance index of A549/DDP cells was 2.77. Compared with A549 cells, A549/DDP cells increased expression of ERCC1 （P 〈 0.05）. Moreover, A549/DDP cells showed morphological and phenotypic changes consistent with EMT： with spindle-shaped morphology, and decreased expression of E-cadherin and increased expression of vimentin. Immunohistochemistry showed significant positive correlation between the expression of ERCCI and vimentin （r = 0.496, 0.332, P 〈 0.05）, and significant negative correlation between the ERCCI and E-cadherin （r = -0.403, -0.295, P 〈 0.05） in neoadjuvant chemotherapy group and simple surgery group. In addition, compared with simple surgery group, the expression of ERCC1 （P = 0.003） and vimentin （P = 0.004） was significantly increased, and the expression of E-cadherin was decreased in neoadjuvant chemotherapy group （P = 0.032）. Cenclusion： A549/DDP cells acquired cisplatin-resistance and occurred EMT simultaneously; the phenomenon of chemoresistance and EMT was caused more easily in neoadjuvant chemotherapy group. As such, we further confirmed the close correlation between chemoresistance and EMT of NSCLC, and provided theoretical basis for the targeting therapy with EMT regulatory factor for chemoresistant NSCLC patients.

Analysis and Prototyping of Multicellular DC-DC Transformer for Environmentally Friendly Data Centers

A multicellular DCX （dc-dc transformer） using unregulated cell converters has been proposed for the environmentally friendly data centers. The high speed cell converter with the switching frequency over MHz behaves as an ideal transformer, and this behavior solves the voltage imbalance issue in the multicellular converter topology. The analysis of the unregulated cell converter is conducted by using the state space averaging method, and the operation condition for the ideal transformer is specified. The behavior of the multicellular DCX using the high speed cell converters has been also analyzed, and the voltage imbalance issue among cell converters is discussed quantitatively. A prototype of a 19.2 kW 384 V-384 V multicellular DCX using sixty-four unregulated cell converters is fabricated and the validity of the analyses is verified.

Primary cutaneous CD30-positive anaplastic large cell lymphoma analysis

OBJECTIVE: To examine 10 cases with primary cutaneous CD30-positive anaplastic large cell lymphoma (ALCL), analyze their clinical manifestations and pathological and immunohistochemical features, and improve early diagnosis of this disease. METHODS: We studied the morphological characteristics of primary cutaneous CD30-positive ALCL using histopathological methods. Leukocyte common antigen (LCA), CD20, CD30, CD45RO, CD68, epithelial membrane antigen (EMA), cytokeratin (CK) and HMB45 antibodies were used to determine the expression of their respective antigens from routine paraffin samples of the patients. RESULTS: Ten patients (7 men and 3 women, aged 31 to 84 years) complained of subcutaneous masses or papular eruptions over their lower trunks and extremities. Histopathologically, the lesions were composed of numerous large round or oval pleomorphic cells. The cytoplasm was usually abundant, amphophilic or basophilic, and finely vacuolated. Nuclei were commonly eccentrically localized and lobated or horseshoed in shape, and multinucleated giant cells and Reed-Sternberg-like cells were seen. Nucleoli were generally multiple and large. Of the 10 patients, tumor cells displayed positive antigen expression of CD30 in all cases, positive CD45RO in 6 cases, positive CD20 in only 1 case, but negative CD45RO and CD20 expressions in 3 cases. Two patients died at 7 weeks and 3.4 years of follow-up, respectively. CONCLUSION: Our study highlights the importance of histopathologic features and positive CD30 staining for differentiation of this disease from other malignant skin tumors.

TRHH-herb pair prevents IL-1β-induced degeneration of endplate chondrocytes in vitro

The inflammatory cytokine interleukin-1 beta （IL-1β） plays a key role in the process of intervertebral disc degenera- tion （IVDD）. In the present study, we aimed to evaluate the effect of pharmaco-serum of ＂Taoren-Honghua-herb pair＂ on IL-1β- induced chondrocyte degeneration in vitro. Taoren （Semen persicae） and Honghua （Safflower carthamus） were administered to the rats, and the pharmaco-serum was collected and prepared. Chondrocytes of the third passage, isolated from the rat＇s vertebral endplates, were treated by standard medium only （Group NC）, IL-1β （Group IL） or combination of IL-1β and pharmaco-serum （Group TRHH）. Cell proliferation and apoptosis were determined, and the expression of aggrecan, Col2ul, Coll0ul, IL-6 and SOX9 at the mRNA level in chondrocytes was quantified by real-time PCR. Immunohistochemistry staining of type II and X collagen and Safranine O staining were also used to evaluate the chondrocytes. Compared with the Group NC, IL-1β treatment inhibited the cell proliferation and induced the cell apoptosis （P〈0.05）, and the expression of aggrecan, Col2αl and SOX9 at the mRNA level was down-regulated. In contrast, the expression of Coll0ul and IL-6 was up-regulated after IL-1β treatment （P〈0.05）. Meanwhile, the immune-staining of type II collagen and Safranine O staining were decreased, while the staining of type X collagen was increased. Compared with the Group IL, cell proliferation was increased, and apoptosis of chondrocytes was decreased when cells were treated with the pharmaco-semm of TRHH-herb pair （P〈0.05）. The expression of aggrecan, Col2cd and SOX9 at the mRNA level was up-regulated, while that of Coll0cd and IL-6 was down-regulated （P〈0.05）. Saffanine O staining also showed increased positive staining （P〈0.05）. Taken together, the treatment of pharmaco-serum of TRHH-herb pair could prevent endplate chondrocyte degeneration induced by IL-1β.

A Poincaré Inequality in a Sobolev Space with a Variable Exponent

Let Ω be a domain in RN. It is shown that a generalized Poincaré inequality holds in cones contained in the Sobolev space Wl,P（ ）（Ω）, where p（.） ： Ω → [1, ∞[ is a variable exponent. This inequality is itself a corollary to a more general result about equivalent norms over such cones. The approach in this paper avoids the difficulty arising from the possible lack of density of the space ;D（Ω） in the space {v ∈ Wl,P（ ）（Ω）; tr v = 0 on δΩ}. Two applications are also discussed.