目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)间质肿瘤浸润淋巴细胞(stromal tumor-infiltrating lymphocytes,sTILs)程序性死亡配体-1(programmed death ligand-1,PD-L1)蛋白的表达及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs...目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)间质肿瘤浸润淋巴细胞(stromal tumor-infiltrating lymphocytes,sTILs)程序性死亡配体-1(programmed death ligand-1,PD-L1)蛋白的表达及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs亚群与临床病理特征的相关性及对患者预后的影响。方法收集遵义医科大学附属医院2015~2019年病理资料完善的女性TNBC标本92例,评估每张HE切片肿瘤区域内sTILs的浸润密度,采用免疫组化EnVision法检测92例TNBC中sTILs的PD-L1蛋白表达情况及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs的浸润密度。结果TNBC中sTILs与Ki-67指数(P=0.001)显著相关;CD4^(+)、CD8^(+)sTILs与肿瘤大小(P=0.012、0.006)和Ki-67指数(P=0.047、0.006)显著相关;Foxp3^(+)sTILs、PD-L1^(+)sTILs与组织学分级(P=0.001、0.005)显著相关。TNBC中sTILs与CD4^(+)、CD8^(+)sTILs呈正相关(P<0.05),与PD-L1^(+)sTILs存在显著相关性(P<0.05),与Foxp3^(+)sTILs无相关性。TNBC预后的单因素和多因素分析发现,PD-L1^(+)sTILs和淋巴结转移阳性患者具有更短的总生存期,而sTILs及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs与患者的预后无相关性。结论TNBC中sTILs及CD4^(+)、CD8^(+)sTILs浸润密度可能与乳腺癌肿瘤细胞增殖有关,Foxp3^(+)sTILs可能与乳腺癌肿瘤细胞分化程度有关,而PD-L1^(+)sTILs和淋巴结转移阳性可能与TNBC的不良预后相关。展开更多
AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were tr...AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were treated with nicotine and periostin protein expression was determined by immunoblotting.Periostin mRNA in gastric cancer cells was silenced using small interfering RNA(siRNA) techniques and periostin gene expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Gastric cancer cells transfected with control or periostin siRNA plasmid were compared in terms of cell proliferation using the methylthiazolyldiphenyl-tetrazolium bromide assay.Cell apoptosis was compared using annexin V-fluoresceine isothiocyanate and propidium iodine double staining.Tumor invasion was determined using the Boyden chamber invasion assay,and the EMT marker Snail expression was evaluated by immunoblotting.RESULTS:Nicotine upregulated periostin in gastric cancer cells through a COX-2 dependent pathway,which was blocked by the COX-2-specific inhibitor NS398.Periostin mRNA expression was decreased by ～87.2% by siRNA in gastric cancer cells,and stable periostinsilenced cells were obtained by G418 screening.Periostin-silenced gastric cancer cells exhibited reduced cell proliferation,elevated sensitivity to chemotherapy with 5-fluorouracil,and decreased cell invasion and Snail expression(P < 0.05).CONCLUSION:Periostin is a nicotine target gene in gastric cancer and plays a role in gastric cancer cell growth,invasion,drug resistance,and EMT facilitated by nicotine.展开更多
Eosinophilic cholangiopathy is a rare condition characterized by eosinophilic infiltration of the biliary tract and causes sclerosing cholangitis. We report a patient with secondary sclerosing cholangitis with eosinop...Eosinophilic cholangiopathy is a rare condition characterized by eosinophilic infiltration of the biliary tract and causes sclerosing cholangitis. We report a patient with secondary sclerosing cholangitis with eosinophilic cholecystitis. A 46-year-old Japanese man was admitted to our hospital with jaundice. Computed tomography revealed dilatation of both the intrahepatic and extrahepatic bile ducts, diffuse thickening of the wall of the extrahepatic bile duct, and thickening of the gallbladder wall. Under the diagnosis of lower bile duct carcinoma, he underwent pyloruspreserving pancreatoduodenectomy and liver biopsy. On histopathological examination, conspicuous fibrosis was seen in the lower bile duct wall. In the gallbladder wall, marked eosinophilic infiltration was seen. Liver biopsy revealed mild portal fibrosis. He was diagnosed as definite eosinophilic cholecystitis with sclerosing cholangitis with unknown etiology. The possible etiology of sderosing cholangitis was consequent fibrosis from previous eosinophilic infiltration in the bile duct. The clinicopathological findings of our case and a literature review indicated that eosinophilic cholangiopathy could cause a condition mimicking primary sclerosing cholangitis （PSC）. Bile duct wall thickening in patients with eosinophilic cholangitis might be due to fibrosis of the bile duct wall. Eosinophilic cholangiopathy might be confused as PSC with eosinophilia.展开更多
文摘目的探讨三阴型乳腺癌(triple negative breast cancer,TNBC)间质肿瘤浸润淋巴细胞(stromal tumor-infiltrating lymphocytes,sTILs)程序性死亡配体-1(programmed death ligand-1,PD-L1)蛋白的表达及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs亚群与临床病理特征的相关性及对患者预后的影响。方法收集遵义医科大学附属医院2015~2019年病理资料完善的女性TNBC标本92例,评估每张HE切片肿瘤区域内sTILs的浸润密度,采用免疫组化EnVision法检测92例TNBC中sTILs的PD-L1蛋白表达情况及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs的浸润密度。结果TNBC中sTILs与Ki-67指数(P=0.001)显著相关;CD4^(+)、CD8^(+)sTILs与肿瘤大小(P=0.012、0.006)和Ki-67指数(P=0.047、0.006)显著相关;Foxp3^(+)sTILs、PD-L1^(+)sTILs与组织学分级(P=0.001、0.005)显著相关。TNBC中sTILs与CD4^(+)、CD8^(+)sTILs呈正相关(P<0.05),与PD-L1^(+)sTILs存在显著相关性(P<0.05),与Foxp3^(+)sTILs无相关性。TNBC预后的单因素和多因素分析发现,PD-L1^(+)sTILs和淋巴结转移阳性患者具有更短的总生存期,而sTILs及CD4^(+)、CD8^(+)、Foxp3^(+)sTILs与患者的预后无相关性。结论TNBC中sTILs及CD4^(+)、CD8^(+)sTILs浸润密度可能与乳腺癌肿瘤细胞增殖有关,Foxp3^(+)sTILs可能与乳腺癌肿瘤细胞分化程度有关,而PD-L1^(+)sTILs和淋巴结转移阳性可能与TNBC的不良预后相关。
基金Supported by Department of Pathology,Division of Basic Medicine,Central South University Xiangya School of Medicine
文摘AIM:To investigate the contribution of periostin in nicotine-promoted gastric cancer cell proliferation,survival,invasion,drug resistance,and epithelial-mesenchymal transition(EMT).METHODS:Gastric cancer cells were treated with nicotine and periostin protein expression was determined by immunoblotting.Periostin mRNA in gastric cancer cells was silenced using small interfering RNA(siRNA) techniques and periostin gene expression was evaluated by quantitative reverse transcription-polymerase chain reaction.Gastric cancer cells transfected with control or periostin siRNA plasmid were compared in terms of cell proliferation using the methylthiazolyldiphenyl-tetrazolium bromide assay.Cell apoptosis was compared using annexin V-fluoresceine isothiocyanate and propidium iodine double staining.Tumor invasion was determined using the Boyden chamber invasion assay,and the EMT marker Snail expression was evaluated by immunoblotting.RESULTS:Nicotine upregulated periostin in gastric cancer cells through a COX-2 dependent pathway,which was blocked by the COX-2-specific inhibitor NS398.Periostin mRNA expression was decreased by ～87.2% by siRNA in gastric cancer cells,and stable periostinsilenced cells were obtained by G418 screening.Periostin-silenced gastric cancer cells exhibited reduced cell proliferation,elevated sensitivity to chemotherapy with 5-fluorouracil,and decreased cell invasion and Snail expression(P < 0.05).CONCLUSION:Periostin is a nicotine target gene in gastric cancer and plays a role in gastric cancer cell growth,invasion,drug resistance,and EMT facilitated by nicotine.
文摘Eosinophilic cholangiopathy is a rare condition characterized by eosinophilic infiltration of the biliary tract and causes sclerosing cholangitis. We report a patient with secondary sclerosing cholangitis with eosinophilic cholecystitis. A 46-year-old Japanese man was admitted to our hospital with jaundice. Computed tomography revealed dilatation of both the intrahepatic and extrahepatic bile ducts, diffuse thickening of the wall of the extrahepatic bile duct, and thickening of the gallbladder wall. Under the diagnosis of lower bile duct carcinoma, he underwent pyloruspreserving pancreatoduodenectomy and liver biopsy. On histopathological examination, conspicuous fibrosis was seen in the lower bile duct wall. In the gallbladder wall, marked eosinophilic infiltration was seen. Liver biopsy revealed mild portal fibrosis. He was diagnosed as definite eosinophilic cholecystitis with sclerosing cholangitis with unknown etiology. The possible etiology of sderosing cholangitis was consequent fibrosis from previous eosinophilic infiltration in the bile duct. The clinicopathological findings of our case and a literature review indicated that eosinophilic cholangiopathy could cause a condition mimicking primary sclerosing cholangitis （PSC）. Bile duct wall thickening in patients with eosinophilic cholangitis might be due to fibrosis of the bile duct wall. Eosinophilic cholangiopathy might be confused as PSC with eosinophilia.
