Objective: To understand the change of total red blood cells(RBCs) in a simulation of microcirculation during the myocardial ischaemia. Methods: The simulation system of capillary blood vessels by silicon microchannel...Objective: To understand the change of total red blood cells(RBCs) in a simulation of microcirculation during the myocardial ischaemia. Methods: The simulation system of capillary blood vessels by silicon microchannels was set up, and the deformability of total RBCs was measured in this system. Results: Compared with the control group, the filtration index(IF), filtration resistance(β) and block probability(ε) in the angina pectoris group increased significantly( P < 0.01 ). Moreover, the temporal parameters of a single cell passing through the microchannels(the time for entering the pore, the time for exiting the pore, the total passage time) also increased markedly. Conclusion: The results indicate that during the myocardial ischaemia, the stiffness and resistance of the RBCs increase, the movement velocity of the RBCs decrease, and the block probability of the RBCs increase in a simulation system of microcirculation.展开更多
Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） ...Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） with attractive luminescent properties may be promising candidates to overcome such challenges. Here, a novel one-step synthetic method is presented to prepare highly fluorescent copper NCs （CuNCs） in ambient conditions by using glutathione （GSH） as both the reducing agent and the protective layer preventing the aggregation of the as-formed NCs. The resultant CuNCs, with an average diameter of 2.3 nm, contain 1-3 atoms and exhibit red fluorescence （A^m = 610 nm） with high quantum yields （QYs, up to 5.0%）. Interestingly, the fluorescence signal of the CuNCs is reversibly responsive to the environmental temperature in the range of 15-80 ℃. Furthermore, as the CuNCs exhibit good biocompatibility, they can pervade the MC3T3-E1 cells and enable measurements over the physiological temperature range of 15-45 ℃ with the use of the confocal fluorescence imaging method. In view of the facile synthesis method and attractive fluorescence properties, the as-prepared CuNCs may be used as photoluminescence thermometers and biosensors.展开更多
Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized ...Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles （MNPs）. Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions （room temperature and physiologic media）. Microscopy （TEM, SEM, AFM, and enhanced dark-field imaging） revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar （two-cell layers） cell sheets and 3D multicellular spheroids.展开更多
文摘Objective: To understand the change of total red blood cells(RBCs) in a simulation of microcirculation during the myocardial ischaemia. Methods: The simulation system of capillary blood vessels by silicon microchannels was set up, and the deformability of total RBCs was measured in this system. Results: Compared with the control group, the filtration index(IF), filtration resistance(β) and block probability(ε) in the angina pectoris group increased significantly( P < 0.01 ). Moreover, the temporal parameters of a single cell passing through the microchannels(the time for entering the pore, the time for exiting the pore, the total passage time) also increased markedly. Conclusion: The results indicate that during the myocardial ischaemia, the stiffness and resistance of the RBCs increase, the movement velocity of the RBCs decrease, and the block probability of the RBCs increase in a simulation system of microcirculation.
基金This work was supported by National Natural Science Foundation of China （Nos. 51372265 and 21175060）, the Natural Science Foundation of Jiangsu Province, China （No. BK20140392）, the Open Foundation of State Key Laboratory of Materials-Oriented Chemical Engineering of Nanjing University of Technology （2014, KL14-12）, the Postdoctoral Research Foundation of Jiangsu Province, China （No. 1401058B）, and the Science and Technology Project of Suzhou, China （Nos~ ZXG201428 and ZXG201401）~
文摘Temperature measurement in biology and medical diagnostics, along with sensitive temperature probing in living cells, is of great importance; however, it still faces significant challenges. Metal nanoclusters （NCs） with attractive luminescent properties may be promising candidates to overcome such challenges. Here, a novel one-step synthetic method is presented to prepare highly fluorescent copper NCs （CuNCs） in ambient conditions by using glutathione （GSH） as both the reducing agent and the protective layer preventing the aggregation of the as-formed NCs. The resultant CuNCs, with an average diameter of 2.3 nm, contain 1-3 atoms and exhibit red fluorescence （A^m = 610 nm） with high quantum yields （QYs, up to 5.0%）. Interestingly, the fluorescence signal of the CuNCs is reversibly responsive to the environmental temperature in the range of 15-80 ℃. Furthermore, as the CuNCs exhibit good biocompatibility, they can pervade the MC3T3-E1 cells and enable measurements over the physiological temperature range of 15-45 ℃ with the use of the confocal fluorescence imaging method. In view of the facile synthesis method and attractive fluorescence properties, the as-prepared CuNCs may be used as photoluminescence thermometers and biosensors.
文摘Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles （MNPs）. Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions （room temperature and physiologic media）. Microscopy （TEM, SEM, AFM, and enhanced dark-field imaging） revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar （two-cell layers） cell sheets and 3D multicellular spheroids.
