In this study,the intestinal microbiota of kuruma shrimp(Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacill...In this study,the intestinal microbiota of kuruma shrimp(Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp.on intestinal microbial diversity.Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp.amended feed.PCR and denaturing gradient gel electrophoresis(DGGE) analyses were then performed on DNA extracted directly from the guts.Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons,and distinct bands in the gels were sequenced.The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp.and uncultured gamma proteobacterium.Overall,the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.展开更多
A novel thermophilic and heterotrophic sulfate-reducing bacteria, strain CW-03, was isolated from crude oil well whose depth was 3.2 kilometer. The bacterium was strictly anaerobic; it does not endure acid and its max...A novel thermophilic and heterotrophic sulfate-reducing bacteria, strain CW-03, was isolated from crude oil well whose depth was 3.2 kilometer. The bacterium was strictly anaerobic; it does not endure acid and its maximum surviving temperature was 70℃. Many short chain organic compounds can be utilized as electron donors, which were acetate, formate, lactate, propionate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, heptanoate, octanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate or ethanol, while sulfate and sulfite were used as electron acceptors. The following substrates were not utilized: benzoate undecanoate, dodecanoate, tetradecane, propanol, butanol, H2+CO2 (80/20%; v/v) and acetate (1mM) + H2. When lactate was used as electron donors, sulfite and thiosulfate, but not sulfer and nitrate, can be used as electron acceptors. Strain CW-03 was motile, curved rod, Gram-positive, pole flagellum and spore-forming. On the basis of 16S rRNA sequence alignment (accession numbers: AY703032), CW-03 should be included in the genus Desulfotomaculum with BlAST analysis on line. However, some of its physiology and multiple sequence alignments were different from other members of this genus. Therefore, CW-03 should be recognized as a new species, for which we propose the name Desulfotomaculum chinamiddle (Bacteria, Firmicutes, Clostridia, Clostridiales, Peptococcaceae).展开更多
基金Supported by the National High Technology Research and Development Program of China(863 Program)(Nos.2006AA100311,2008AA100805)
文摘In this study,the intestinal microbiota of kuruma shrimp(Marsupenaeus japonicus) was examined by molecular analysis of the 16S rDNA to identify the dominant intestinal bacteria and to investigate the effects of Bacillus spp.on intestinal microbial diversity.Samples of the intestines of kuruma shrimp fed normal feed and Bacillus spp.amended feed.PCR and denaturing gradient gel electrophoresis(DGGE) analyses were then performed on DNA extracted directly from the guts.Population fingerprints of the predominant organisms were generated by DGGE analysis of the universal V3 16S rDNA amplicons,and distinct bands in the gels were sequenced.The results suggested that the gut of kuruma shrimp was dominated by Vibrio sp.and uncultured gamma proteobacterium.Overall,the results of this study suggest that PCR-DGGE is a possible method of studying the intestinal microbial diversity of shrimp.
文摘A novel thermophilic and heterotrophic sulfate-reducing bacteria, strain CW-03, was isolated from crude oil well whose depth was 3.2 kilometer. The bacterium was strictly anaerobic; it does not endure acid and its maximum surviving temperature was 70℃. Many short chain organic compounds can be utilized as electron donors, which were acetate, formate, lactate, propionate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, heptanoate, octanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate or ethanol, while sulfate and sulfite were used as electron acceptors. The following substrates were not utilized: benzoate undecanoate, dodecanoate, tetradecane, propanol, butanol, H2+CO2 (80/20%; v/v) and acetate (1mM) + H2. When lactate was used as electron donors, sulfite and thiosulfate, but not sulfer and nitrate, can be used as electron acceptors. Strain CW-03 was motile, curved rod, Gram-positive, pole flagellum and spore-forming. On the basis of 16S rRNA sequence alignment (accession numbers: AY703032), CW-03 should be included in the genus Desulfotomaculum with BlAST analysis on line. However, some of its physiology and multiple sequence alignments were different from other members of this genus. Therefore, CW-03 should be recognized as a new species, for which we propose the name Desulfotomaculum chinamiddle (Bacteria, Firmicutes, Clostridia, Clostridiales, Peptococcaceae).
