Use of 16S-23S intergenic transcribed spacer (ITS) variability,as a relatively new method,is becoming an important supplement to the molecular methods based on 16S rRNA for which has a fairly constant size and is not ...Use of 16S-23S intergenic transcribed spacer (ITS) variability,as a relatively new method,is becoming an important supplement to the molecular methods based on 16S rRNA for which has a fairly constant size and is not divergent enough to give good separation in close relationships. This paper summarizes the structures and characteristics of ITS regions that are extremely variable in copy number,length and sequence per genome. The ITS region can be amplified easily taking advantage of conserved nucleotide stretches at the 5’ of the 16S and 3’ of the 23S gene,and the amplicon can contain different amounts of the 16S rDNA by choosing primers at different conserved areas within this gene. These primers are listed and discussed for perfecting the methodology of ITS. Furthermore,some recent progresses on the taxonomy,identification and community analysis of bacteria by means of ITS in epidemiology,ecology and artificial environment are reviewed,as well,the virtues and limitations of that method are discussed. Fig 2,Tab 1,Ref展开更多
细菌的分类鉴定与细菌的研究和应用有着同等重要的地位。目前从自然界中可以培养的细菌总量不到1%,大多数海洋细菌的分类鉴定仍需建立在纯培养基础上。本文对目前可培养海洋细菌的分类鉴定方法做了系统的综述,详述了系统发生树的构建,...细菌的分类鉴定与细菌的研究和应用有着同等重要的地位。目前从自然界中可以培养的细菌总量不到1%,大多数海洋细菌的分类鉴定仍需建立在纯培养基础上。本文对目前可培养海洋细菌的分类鉴定方法做了系统的综述,详述了系统发生树的构建,汇总了生理生化分类特征,概述了主要的遗传学分析方法,包括16S rDNA序列分析,G+C含量测定,DNA-DNA杂交,MLST(多位点序列分型,Multi Locus Sequence Typing)以及核酸指纹图谱等,并指出每种方法适用的范围以及应用时需要注意的事项。另外,本文还提出了新菌分类鉴定的一般步骤。文章最后对目前海洋细菌分类鉴定的发展趋势进行了总结和展望。展开更多
文摘Use of 16S-23S intergenic transcribed spacer (ITS) variability,as a relatively new method,is becoming an important supplement to the molecular methods based on 16S rRNA for which has a fairly constant size and is not divergent enough to give good separation in close relationships. This paper summarizes the structures and characteristics of ITS regions that are extremely variable in copy number,length and sequence per genome. The ITS region can be amplified easily taking advantage of conserved nucleotide stretches at the 5’ of the 16S and 3’ of the 23S gene,and the amplicon can contain different amounts of the 16S rDNA by choosing primers at different conserved areas within this gene. These primers are listed and discussed for perfecting the methodology of ITS. Furthermore,some recent progresses on the taxonomy,identification and community analysis of bacteria by means of ITS in epidemiology,ecology and artificial environment are reviewed,as well,the virtues and limitations of that method are discussed. Fig 2,Tab 1,Ref
文摘细菌的分类鉴定与细菌的研究和应用有着同等重要的地位。目前从自然界中可以培养的细菌总量不到1%,大多数海洋细菌的分类鉴定仍需建立在纯培养基础上。本文对目前可培养海洋细菌的分类鉴定方法做了系统的综述,详述了系统发生树的构建,汇总了生理生化分类特征,概述了主要的遗传学分析方法,包括16S rDNA序列分析,G+C含量测定,DNA-DNA杂交,MLST(多位点序列分型,Multi Locus Sequence Typing)以及核酸指纹图谱等,并指出每种方法适用的范围以及应用时需要注意的事项。另外,本文还提出了新菌分类鉴定的一般步骤。文章最后对目前海洋细菌分类鉴定的发展趋势进行了总结和展望。
