In biology ferment engineering,accurate statistics of the quantity of bacteria is one of the most important subjects. In this paper,the quantity of bacteria which was observed traditionally manuauy can be detected aut...In biology ferment engineering,accurate statistics of the quantity of bacteria is one of the most important subjects. In this paper,the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and processing system is designed to accomplish image preprocessing,image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully realized by means of a region-based level set method and then the quantity of bacteria is computed precisely,which plays an important role in optimizing the growth conditions of bacteria.展开更多
Fluorescence correlation spectroscopy （FCS） without objective image magnification （without using con-focal microscope） was applied to observe the variation in cell size of Escherichia coli （E. coli） induced by t...Fluorescence correlation spectroscopy （FCS） without objective image magnification （without using con-focal microscope） was applied to observe the variation in cell size of Escherichia coli （E. coli） induced by the anti-cancer agent MitomycinC （MMC）. In the system without image magnification followed in this study, the suspension of E. coli cells was stirred, and the difference in movement due to the different cell sizes induced by the compulsive solution flow was detected. The addition of 0.1-0.4 pg/L of MMC elongated the E. coli cell length from about 3.6 to 7.8μm. The flow cell （i.d. = about 1 mm） also produced a size-dependent correlation curve, The present system is not based on single molecular FCS but is inexpensive and effective at observing the variation in cell size induced by environmental changes.展开更多
基金863 Programgrant number:2007AA02Z211+3 种基金Jiangsu Science and Technology Departmentgrant number:BE2008399Education of Jiangsu Provincegrant number:08KJA530002
文摘In biology ferment engineering,accurate statistics of the quantity of bacteria is one of the most important subjects. In this paper,the quantity of bacteria which was observed traditionally manuauy can be detected automatically. Image acquisition and processing system is designed to accomplish image preprocessing,image segmentation and statistics of the quantity of bacteria. Segmentation of bacteria images is successfully realized by means of a region-based level set method and then the quantity of bacteria is computed precisely,which plays an important role in optimizing the growth conditions of bacteria.
文摘Fluorescence correlation spectroscopy （FCS） without objective image magnification （without using con-focal microscope） was applied to observe the variation in cell size of Escherichia coli （E. coli） induced by the anti-cancer agent MitomycinC （MMC）. In the system without image magnification followed in this study, the suspension of E. coli cells was stirred, and the difference in movement due to the different cell sizes induced by the compulsive solution flow was detected. The addition of 0.1-0.4 pg/L of MMC elongated the E. coli cell length from about 3.6 to 7.8μm. The flow cell （i.d. = about 1 mm） also produced a size-dependent correlation curve, The present system is not based on single molecular FCS but is inexpensive and effective at observing the variation in cell size induced by environmental changes.
