目的评价质子泵抑制剂（PPI）与小肠细菌过度增长之间的关系。方法检索MEDLINE ／PubMed、Cochrane Central Register of Controlled Trials、中国生物医学文献数据库等，按标准纳入 PPI 导致细菌过度增长的所有观察性研究。采用 Cochra...目的评价质子泵抑制剂（PPI）与小肠细菌过度增长之间的关系。方法检索MEDLINE ／PubMed、Cochrane Central Register of Controlled Trials、中国生物医学文献数据库等，按标准纳入 PPI 导致细菌过度增长的所有观察性研究。采用 Cochrane 协作网提供的 RevMan 5．1软件进行荟萃分析。结果8项观察性研究共576例患者纳入分析。结果显示，PPI 显著增加发生胃肠道细菌过度增长的风险（阳性率为46．61％），与对照组比较差异有统计学意义[风险比（OR）＝13．46，95％可信区间（CI）：7．00～25．88，P ＜0．01]；不同剂量 PPI 比较，20 mg／d 和40 mg／d 组细菌过度增长的阳性率分别为47．62％和39．62％；不同疗程 PPI 比较，疗程＞1个月组与对照组差异有统计学意义（OR ＝16．47％，95％CI：7．82～34．71，P ＜0．01），而疗程＜1个月组与对照组差异无统计学意义（P ＞0．05）；细菌培养组和葡萄糖氢呼气试验组，细菌过度增长的阳性率分别为48．03％和45．87％，与对照组比较差异均有统计学意义（P ＜0．01）。结论PPI 可增加胃肠道发生细菌过度增长的风险，但短期治疗可能不影响胃酸阻止细菌定殖胃肠道的功能。展开更多
The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathwa...The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways.Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK),PKA inhibitor (H89),PKC activator (PMA) or inhibitor (H7).The germ cell number was counted from micropictures.The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells.The germ cell labeling index (LI) was determined for cell proliferation.The FSH treatment increased the germ cell number,and this stimulating effect was enhanced by FRSK or PMA,but inhibited by H89 or H7 in a dose-dependent manner.Moreover,the PCNA-LI showed parallel changes with germ cell numbers.This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.展开更多
基金Project supported by the National Natural Science Foundation of China (No. 30871843)the Key Project of the Science and Technology Department of Zhejiang Province, China (No. 2008C12050)
文摘The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) pathways.Ovarian cells were treated with FSH alone or in the presence of forskolin (FRSK),PKA inhibitor (H89),PKC activator (PMA) or inhibitor (H7).The germ cell number was counted from micropictures.The immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating cells.The germ cell labeling index (LI) was determined for cell proliferation.The FSH treatment increased the germ cell number,and this stimulating effect was enhanced by FRSK or PMA,but inhibited by H89 or H7 in a dose-dependent manner.Moreover,the PCNA-LI showed parallel changes with germ cell numbers.This study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.
