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鼠伤寒沙门菌细菌影负载防龋DNA疫苗对小鼠黏膜免疫效能的影响 被引量:2
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作者 马琼 李玲 +2 位作者 孙聪 邓春妮 马丽 《临床和实验医学杂志》 2019年第6期584-587,共4页
目的分析鼠伤寒沙门菌(St)细菌影负载防龋DNA疫苗对黏膜免疫效能的影响。方法收集St减毒株J357,同时转入噬菌体Phi X基因E表达质粒与pREP4质粒,加入异丙基-β-D-硫代半乳糖苷(IPTG)进行诱导,收集负载防龋DNA疫苗。按照随机原则,将20只SP... 目的分析鼠伤寒沙门菌(St)细菌影负载防龋DNA疫苗对黏膜免疫效能的影响。方法收集St减毒株J357,同时转入噬菌体Phi X基因E表达质粒与pREP4质粒,加入异丙基-β-D-硫代半乳糖苷(IPTG)进行诱导,收集负载防龋DNA疫苗。按照随机原则,将20只SPF级BALB/c雌性小鼠分为A组(布比卡因包裹p VXA1 5μg)、B组(布比卡因包裹p GJGLU/VAX 5μg)、C组(细菌影负载空载体p VXA1 5μg)、D组(细菌影负载防龋DNA疫苗p GJGLU/VAX 5μg)共4组经鼻免疫小鼠,每组均为5只。采用酶联免疫吸附试验对各组小鼠唾液抗体的产生情况进行检测。结果经IPTG诱导后,对照组细菌生长速度较快;而表达基因E经IPTG诱导后,大量细菌出现死亡,随着诱导时间的延长,细菌的死亡数量明显进一步增多。经IPTG诱导前,取携带基因E的St中J357活菌数为2×1012个/L;而经异丙基-β-D-硫代半乳糖苷(IPTG)诱导后,其活菌数仅为5×108个/L,表明大量细菌被杀死。经酶联免疫吸附试验检测结果发现,唾液总Ig A抗体水平在各组小鼠免疫前的比较,并无显著差异(P> 0. 05);相比A组,C、D组小鼠免疫后唾液总Ig A抗体水平均明显升高(P <0. 05);而C、D组唾液总Ig A抗体水平与B组比较,均无显著差异(P> 0. 05)。经酶联免疫吸附试验检测结果发现,各组小鼠免疫前均未检出唾液特异性抗葡聚糖结合区Ig A抗体;免疫后8周,D组唾液特异性抗葡聚糖结合区Ig A抗体水平较A、B、C组均明显升高(P <0. 05),而B组抗体水平与A、C组比较,均无显著差异(P> 0. 05)。结论经鼻黏膜途径免疫小鼠经St细菌影负载防龋DNA疫苗后可明显改善其免疫效能。 展开更多
关键词 小鼠 鼠伤寒沙门菌 细菌影 龋齿 DNA疫苗
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细菌影载体负载防龋DNA疫苗免疫小鼠的效果研究 被引量:6
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作者 刘高霞 樊明文 郭继华 《中华口腔医学杂志》 CAS CSCD 北大核心 2014年第1期37-41,共5页
目的研制鼠伤寒沙门菌细菌影,负载防龋DNA疫苗,探寻增强防龋DNA疫苗黏膜免疫效能的方法.方法将pREP4质粒和噬菌体PhiX基因E表达质粒同时转入鼠伤寒沙门菌减毒株J357中,加入异丙基硫代半乳糖苷(isopropylβ-D-1-thiogalactopyranoside,I... 目的研制鼠伤寒沙门菌细菌影,负载防龋DNA疫苗,探寻增强防龋DNA疫苗黏膜免疫效能的方法.方法将pREP4质粒和噬菌体PhiX基因E表达质粒同时转入鼠伤寒沙门菌减毒株J357中,加入异丙基硫代半乳糖苷(isopropylβ-D-1-thiogalactopyranoside,IPTG)诱导,收集洗涤,负载防龋DNA疫苗,分4组经鼻免疫小鼠,分别为:细菌影+pGJGLU/VAX组,细菌影负载5μg防龋DNA疫苗pGJGLU/VAX;细菌影+pVAX1组,细菌影负载5μg空载体pVAX1;pGJGLU/VAX组,布比卡因包裹5μgpGJGLU/VAX;pVAX1组,布比卡因包裹5μgpVAX1.酶联免疫吸附测定(enzyme-linkedimmunosorbentassay,ELISA)法检测唾液抗体产生结果.结果ELISA结果显示经鼻黏膜免疫鼠伤寒沙门菌J357细菌影负载的防龋DNA疫苗pGJGLU/VAX(细菌影+pGJGLU/VAX组)后,诱导了显著的唾液特异性抗葡聚糖结合区的IgA抗体,(x)&#177;sx为(0.367&#177;0.086)A/μg,与细菌影+pVAX1组[(0.122&#177;0.077)A/μg]、pGJGLU/VAX组[(0.068&#177;0.068)A/μg]和pVAX1组[(0.089&#177;0.089)A/μg]相比,差异均有统计学意义(P值分别为0.028、0.012和0.030).结论成功制备了鼠伤寒沙门菌细菌影,负载防龋DNA疫苗后经鼻黏膜途径免疫小鼠能有效提高免疫效能. 展开更多
关键词 龋齿 沙门菌 鼠伤寒 细菌影
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大肠杆菌“细菌膜影”的制备与初步研究 被引量:2
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作者 王宁 曾韦锟 +1 位作者 郭刚 邹全明 《解放军医学杂志》 CAS CSCD 北大核心 2005年第3期226-228,共3页
目的 利用热诱导方法制备大肠杆菌的“细菌膜影”(bacterialghost),并鉴定其裂解效率及形态。方法 将含有裂解质 粒pMuH36的大肠杆菌DH5α进行温度诱导,温度从37℃突然升至42℃,每30min检测菌液的OD值,并于诱导4h后收集菌体,通过 菌... 目的 利用热诱导方法制备大肠杆菌的“细菌膜影”(bacterialghost),并鉴定其裂解效率及形态。方法 将含有裂解质 粒pMuH36的大肠杆菌DH5α进行温度诱导,温度从37℃突然升至42℃,每30min检测菌液的OD值,并于诱导4h后收集菌体,通过 菌落计数(CFU)计算裂解率;用透射电镜观察裂解后的细菌形态。结果 通过温度诱导,DH5α(pMuH36)的OD值在诱导1h后开始 持续下降,4h后又开始上升,于4h时收集菌体裂解率为95%,透射电镜观察绝大部分为形态完好的细菌外膜,即“细菌膜影”。结论  本研究高效制备和鉴定了大肠杆菌的“细菌膜影”,为研发更有效的“细菌膜影”疫苗和佐剂奠定了基础。 展开更多
关键词 大肠杆菌 细菌 疫苗 佐剂
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细菌菌影疫苗的研究进展 被引量:3
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作者 江银波 曾铁兵 《微生物学免疫学进展》 2016年第3期72-75,共4页
细菌菌影疫苗(Vaccine of bacterial ghost)作为一种新型疫苗,既可诱导机体产生细胞免疫,又能诱导机体产生体液免疫和黏膜免疫。近年来,利用细菌菌影递送载体的特性,构建重组多价疫苗、DNA疫苗及药物递送的应用越来越受到人们的重视。
关键词 细菌 疫苗 递呈 免疫
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缩节胺和硝普钠对棉花幼苗根际土壤酶活性及细菌群落的影响
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作者 吴建飞 刘茜 +3 位作者 李璇 温天旺 杨文亭 汤飞宇 《棉花学报》 CSCD 北大核心 2023年第5期353-364,共12页
【目的】研究棉花幼苗施用缩节胺(1,1-dimethyl piperidinium chloride,DPC)和一氧化氮供体硝普钠(sodium nitroprusside,SNP)后根际土壤酶活性及细菌群落的变化,探讨与棉花幼苗生长相关的根际生物学指标。【方法】采用陆地棉品系A201... 【目的】研究棉花幼苗施用缩节胺(1,1-dimethyl piperidinium chloride,DPC)和一氧化氮供体硝普钠(sodium nitroprusside,SNP)后根际土壤酶活性及细菌群落的变化,探讨与棉花幼苗生长相关的根际生物学指标。【方法】采用陆地棉品系A201进行穴盘育苗试验。于棉花1叶1心期分别在叶片均匀涂抹50 mg·L^(-1)DPC、500μmol·L^(-1)SNP,以涂抹去离子水为对照。于3叶1心期取根际土壤分析蔗糖酶、脲酶、过氧化氢酶、碱性磷酸酶活性,采用16S rRNA测序技术分析根际细菌多样性。【结果】DPC和SNP处理显著促进棉花根系生长,显著提高茎粗和植株干物质质量。DPC处理显著提高土壤脲酶和蔗糖酶的活性,SNP处理显著提高土壤蔗糖酶活性但显著降低脲酶的活性,但是DPC和SNP处理均未显著影响过氧化氢酶和碱性磷酸酶活性。DPC处理显著提高髌骨菌门(Patescibacteria)的相对丰度和土壤细菌群落的辛普森多样性指数(Simpson's diversity index),显著降低了绿弯菌门(Chloroflexi)和酸杆菌门(Acidobacteria)的相对丰度以及土壤细菌群落的香农-维纳多样性指数(Shannon Wiener’s diversity index)。冗余分析显示,DPC处理组的酵母菌科(Saccharimonadaceae)、TM7a属细菌的相对丰度比SNP处理组和对照组高;SNP处理组的纤维弧菌属(Cellvibrionaceae)细菌相对丰度高于DPC处理组和对照组;DPC和SNP处理组的根瘤菌科(Rhizobiaceae)细菌的相对丰度高于对照组,硝化螺旋菌科(Nitrospiraceae)和硝化螺旋菌属(Nitrospira)细菌的相对丰度低于对照组。脲酶活性与酵母菌科、TM7a属细菌的相对丰度呈显著正相关关系。【结论】DPC和SNP能够促进棉花幼苗根系及植株生长,并影响棉苗根际的土壤酶活性及细菌群落结构。 展开更多
关键词 棉花 缩节胺 硝普钠 土壤酶 细菌群落 丰度 多样性
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细菌菌影在DNA疫苗研究中的作用 被引量:6
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作者 靳小攀 季守平 《中国生物工程杂志》 CAS CSCD 北大核心 2010年第7期92-96,共5页
免疫应答水平低下是制约DNA疫苗发展的一个障碍。细菌菌影(bacterialghost,BG)是利用φX174噬菌体的裂解蛋白将革兰氏阴性细菌裂解后形成的空腔,它保留了细菌结构的完整性,具有免疫佐剂的特性,可以作为递送载体,靶向性的将DNA疫苗导入... 免疫应答水平低下是制约DNA疫苗发展的一个障碍。细菌菌影(bacterialghost,BG)是利用φX174噬菌体的裂解蛋白将革兰氏阴性细菌裂解后形成的空腔,它保留了细菌结构的完整性,具有免疫佐剂的特性,可以作为递送载体,靶向性的将DNA疫苗导入到抗原递呈细胞,从而提高DNA疫苗的免疫应答水平,此外,装载核酸疫苗的细菌菌影可以通过多种方式进行免疫,例如,肌肉注射、皮下注射、口服、黏膜免疫等,更是从根本上提高了DNA疫苗的免疫水平,因此可以说BG是一个极具潜力的核酸疫苗递送载体。现就BG的特性及其在DNA疫苗递送载体中应用的最新进展做一综述。 展开更多
关键词 DNA疫苗 细菌 革兰氏阴性细菌
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细菌菌影作为新型疫苗和递送载体的研究进展 被引量:4
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作者 杨慧 焦红梅 《中国生物工程杂志》 CAS CSCD 北大核心 2014年第9期108-112,共5页
细菌菌影(bacterial ghost,BG)是革兰阴性生菌在噬菌体PhiX174的裂解基因E的作用下形成不含核酸、核糖体等胞质内容物的细菌空壳。这种细菌空壳保留了与天然细菌一样的完整外膜结构,且不具有活菌样的致病作用,可作为疫苗无需佐剂就能诱... 细菌菌影(bacterial ghost,BG)是革兰阴性生菌在噬菌体PhiX174的裂解基因E的作用下形成不含核酸、核糖体等胞质内容物的细菌空壳。这种细菌空壳保留了与天然细菌一样的完整外膜结构,且不具有活菌样的致病作用,可作为疫苗无需佐剂就能诱导机体产生体液免疫应答和细胞免疫应答。菌影内部及外膜上可装载DNA、抗原和药物等异源物质,易被机体免疫细胞识别捕获,使其成为一种新型的生物递送载体。另外,菌影具有制备简单,易于保存等优点。 展开更多
关键词 细菌 疫苗 递送载体
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新型肠炎沙门菌菌影候选疫苗在小鼠免疫应答和保护功效的评估
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作者 杨慧 韩雪 +2 位作者 杜小春 焦红梅 郑国军 《中华微生物学和免疫学杂志》 CAS CSCD 北大核心 2024年第4期295-303,共9页
目的比较肠炎沙门菌制备的菌影(SE-ghost)通过肌肉注射(肌注)和口服不同免疫途径接种雌性BALB/c小鼠诱导体液和细胞免疫应答的功效。方法用SE-ghost接种BALB/c小鼠,间接ELISA检测小鼠血清中IgG抗体水平和阴道灌洗液IgA抗体水平;流式细... 目的比较肠炎沙门菌制备的菌影(SE-ghost)通过肌肉注射(肌注)和口服不同免疫途径接种雌性BALB/c小鼠诱导体液和细胞免疫应答的功效。方法用SE-ghost接种BALB/c小鼠,间接ELISA检测小鼠血清中IgG抗体水平和阴道灌洗液IgA抗体水平;流式细胞仪检测小鼠脾脏CD3^(+)CD4^(+)T、CD3^(+)CD8^(+)T细胞百分比变化和脾脏内细胞因子IL-4、IFN-γ分泌;三次免疫后所有小鼠口服半数致死量肠炎沙门菌进行强毒攻击。用SE-ghost刺激小鼠骨髓来源树突状细胞(BMDCs),流式细胞仪检测表面分子抗体APC-CD11c、FITC-MHC ClassⅡ、PE-CD40、FITC-CD86、PE-CD80表达量;双抗体夹心ELISA测定BMDCs上清液IL-1β、IL-6、IL-10和IL-12p70的产量。结果与PBS组相比,SE-ghost(口服)和SE-ghost(肌注)免疫组小鼠体内肠炎沙门菌特异性抗体IgG和IgA水平明显增高;脾脏CD3^(+)CD4^(+)T细胞百分比上调、高分泌细胞因子IL-4、IFN-γ。肌肉注射SE-ghost比口服可诱导更强的体液和细胞免疫应答。致病菌肠炎沙门菌强毒攻击后,SE-ghost(口服)和SE-ghost(肌注)免疫组生存率高达80%。与对照组相比,BMDCs经SE-ghost刺激后高表达表面成熟分子抗体CD86、CD80、CD40、MHCⅡ,高分泌细胞因子IL-1β、IL-6、IL-10、IL-12p70。结论通过肌肉注射SE-ghost进行免疫,可在小鼠中引发更强大的抗原特异性免疫应答,以防止沙门菌感染。SE-ghost是一种有用的沙门菌候选疫苗。 展开更多
关键词 肠炎沙门菌 细菌 灭活疫苗 免疫应答 保护功效
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The Short-term Effects of Temperature and Free Ammonia on Ammonium Oxidization in Granular and Floccular Nitrifying System 被引量:8
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作者 吴蕾 彭永臻 +3 位作者 马勇 刘旭 李凌云 王淑莹 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2012年第5期1016-1023,共8页
The short-term effects of temperature and free ammonia (FA) on ammonium oxidization were investigated in this study by operating several batch tests with two different partial nitrification aggregates, formed as eithe... The short-term effects of temperature and free ammonia (FA) on ammonium oxidization were investigated in this study by operating several batch tests with two different partial nitrification aggregates, formed as either granules or flocs. The results showed that the rate of ammonium oxidation in both cultures increased significantly as temperature increased from 10 to 30 °C. The specific ammonium oxidation rate with the granules was 2-3 times higher than that with flocs at the same temperature. Nitrification at various FA concentrations and temperatures combination exhibited obvious inhibition in ammonium oxidation rate when FA was 90 mg·L 1 and tempera- ture dropped to 10 °C in the two systems. However, the increase in substrate oxidation rate of ammonia at 30 °C was observed. The results suggested that higher reaction temperature was helpful to reduce the toxicity of FA. Granules appeared to be more tolerant to FA attributed to the much fraction of ammonia oxidizing bacteria (AOB) and higher resistance to the transfer of ammonia into the bacterial aggregates, whereas in the floc system, the bacteria distributed throughout the entire aggregate. These results may contribute to the applicability of the nitrifying granules in wastewater treatment operated at high ammonium concentration. 展开更多
关键词 nitrifying granules TEMPERATURE free ammonia ammonium oxidization activation energy
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Sickle cell cholangiopathy:An endoscopic retrograde cholangiopancreatography evaluation 被引量:1
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作者 Hussain Issa Ali Al-Haddad Ahmed Al-Salem 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第42期5316-5320,共5页
AIM:To evaluate the role of endoscopic retrograde cholangiopancreatography(ERCP) in patients with sickle cell disease(SCD) .METHODS:Two hundred and twenty four SCD patients with cholestatic jaundice(CJ) had ERCP.The i... AIM:To evaluate the role of endoscopic retrograde cholangiopancreatography(ERCP) in patients with sickle cell disease(SCD) .METHODS:Two hundred and twenty four SCD patients with cholestatic jaundice(CJ) had ERCP.The indications for ERCP were based on clinical and biochemical evidence of CJ and ultrasound findings.RESULTS:Two hundred and forty ERCPs were performed.The indications for ERCP were:CJ only in 79,CJ and dilated bile ducts without stones in 103,and CJ and bile duct stones in 42.For those with CJ only,ERCP was normal in 42(53.2%) ,and 13(16.5%) had dilated bile ducts without an obstructive cause.In the remaining 22,there were bile duct stones with or without dilation.For those with CJ,dilated bile ducts and no stones,ERCP was normal in 17(16.5%) ,and 28(27.2%) had dilated bile ducts without an obstructive cause.In the remaining 58,there were bile ducts stones with or without dilation.For those with CJ and bile duct stones,ERCP was normal in two(4.8%) ,and 14(33.3%) had dilated bile ducts without an obstructive cause.In the remaining 26,there were bile duct stones with or without dilatation.CONCLUSION:Considering the high frequency of biliary sludge and bile duct stones in SCD,endoscopic sphincterotomy might prove helpful in these patients. 展开更多
关键词 Sickle cell disease HEPATOBILIARY Cholestsatic jaundice Sickle cell hepatopathy Sickle cell cholangiopathy Endoscopic retrograde cholangiopancreatography
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Bacterial degradation of anthraquinone dyes 被引量:6
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作者 Hai-hong LI Yang-tao WANG +3 位作者 Yang WANG Hai-xia WANG Kai-kai SUN Zhen-mei LU 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2019年第6期528-540,共13页
Anthraquinone dyes,which contain anthraquinone chromophore groups,are the second largest class of dyes after azo dyes and are used extensively in textile industries.The majority of these dyes are resistant to degradat... Anthraquinone dyes,which contain anthraquinone chromophore groups,are the second largest class of dyes after azo dyes and are used extensively in textile industries.The majority of these dyes are resistant to degradation because of their complex and stable structures;consequently,a large number of anthraquinone dyes find their way into the environment causing serious pollution.At present,the microbiological approach to treating printing and dyeing wastewater is considered to be an economical and feasible method,and reports regarding the bacterial degradation of anthraquinone dyes are increasing.This paper reviews the classification and structures of anthraquinone dyes,summarizes the types of degradative bacteria,and explores the possible mechanisms and influencing factors of bacterial anthraquinone dye degradation.Present research progress and existing problems are further discussed.Finally,future research directions and key points are presented. 展开更多
关键词 Anthraquinone dyes Bacterial degradation Degradation mechanism Influencing factor
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Blooming of bacteria and algae is a biokiller for mass-extinction of Devonian coral-stromatoporoid reef ecosystems 被引量:2
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作者 WU YiBu FENG Qi GONG YiMing 《Science China Earth Sciences》 SCIE EI CAS 2013年第7期1221-1232,共12页
Studies of rock slices showed that there were many kinds of symbioses between bacteria and algae and corals-stromatoporoids in the coral-stromatoporoid reefs from the Devonian Ganxi section of Sichuan and the Dushan s... Studies of rock slices showed that there were many kinds of symbioses between bacteria and algae and corals-stromatoporoids in the coral-stromatoporoid reefs from the Devonian Ganxi section of Sichuan and the Dushan section of Guizhou in South China. They included encrustations, microborings, bioclaustration, etc. In the host corals-stromatoporoids invaded by bacteria and algae were many residues of dead soft issue in the infected parts, where the skeletal structures were injured. Therefore, we considered there were direct interactions between corals-stromatoporoids and bacteria and algae in coral-stromatoporoid reefs, which included that bacteria and algae blocked growth of corals-stromatoporoids and the latter had the ability of self-healing. And the bacteria and algae usually was the active side. In the Middle Devonian with normal seawater condition, corals and stromatoporoids had the ability to resist the invasion of bacteria and algae, and the host coral-stromatoporoids would not be killed; but in the Late Devonian with deterioration of seawater quality, the ability of bacteria and algae infection increased while corals-stromatoporoids' ability to resist infection declined, and therefore the host corals-stromatoporoids would be dead. Hence we suggested that the invading of bacteria and algae was a possible biokiller for mass-extinction of the Devonian coral-stromatoporoid reefs ecosystem. Beyond that, blooming of bacteria and algae and its triggering cumulative environmental effects played an important role in the reduction and extinction of metazoan in the Late Devonian. Furthermore, it can be used as a useful example to learn the trend and the reasons for the disease and decrease of modern coral reefs. 展开更多
关键词 bacteria and algae coral-stromatoporoid reefs DEVONIAN mass-extinction South China
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