Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bacterial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1×10...Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bacterial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1×10 8 cfu·mL -1 Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A 2 (PLA 2) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor α(TNF α), inferleukin 6 (IL 6) and plasma thromboxane B 2 (TXB 2), 6 keto platelet growth factor 1 (PGF 1α ) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA 2, TNF α, IL 6 and plasma TXB 2 levels increased significantly in model group ( P <0.01) while those of DBQ groups decreased significantly( P <0 01). Conclusion DBQ dramatically inhibits the overproduction of pro inflammatory factor PLA 2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti inflammatory and protective effect against acute bacterial cholangitis in rabbits has been revealed.展开更多
基金SupportedbyTianjinNinth Five YearProject,No .983 113 411
文摘Aim To study the mechanism of protective effects of Danbiqing granule (DBQ) on experimental acute bacterial cholangitis in rabbits. Methods The acute bacterial cholangitis was induced by injecting 1 mL of 1×10 8 cfu·mL -1 Escherchia coli suspension into common bile duit. The serum nitrous oxide (NO) levels were measured using nitric acid reductase kit. Phospholipase A 2 (PLA 2) activity was assayed by a method of acid titration (microassay). Serum tumor necrcsis factor α(TNF α), inferleukin 6 (IL 6) and plasma thromboxane B 2 (TXB 2), 6 keto platelet growth factor 1 (PGF 1α ) were determined by radioimmunoassay. Results Compared with control group, serum NO, PLA 2, TNF α, IL 6 and plasma TXB 2 levels increased significantly in model group ( P <0.01) while those of DBQ groups decreased significantly( P <0 01). Conclusion DBQ dramatically inhibits the overproduction of pro inflammatory factor PLA 2 and inflammatory cytokine. Hence, the mechanism of DBQ underlying anti inflammatory and protective effect against acute bacterial cholangitis in rabbits has been revealed.
