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CO_2气腹对肠道菌群生物学特性影响的实验研究 被引量:3
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作者 周丁华 卫冰 +1 位作者 李宁 黎介寿 《世界华人消化杂志》 CAS 2003年第10期1649-1651,共3页
目的:探讨CO_2气腹对肠道菌群生物学特性的影响。方法:采用细菌显微培养、通用引物PCR及细菌形态学检测技术对CO_2气腹后大鼠肠道菌群进行细菌鉴定,并观察其繁殖及群集性能结果:CO_2气腹后肠道细菌繁殖能力最强的仍是大肠杆菌、乳酸杆... 目的:探讨CO_2气腹对肠道菌群生物学特性的影响。方法:采用细菌显微培养、通用引物PCR及细菌形态学检测技术对CO_2气腹后大鼠肠道菌群进行细菌鉴定,并观察其繁殖及群集性能结果:CO_2气腹后肠道细菌繁殖能力最强的仍是大肠杆菌、乳酸杆菌、双歧杆菌和肠球菌,并无特异性菌株生长与繁殖。随着CO_2气腹持续时间的延长及压力的增高,肠道细菌群集率、细菌潜生体检出率逐渐增大。CO_2气腹持续2h后,肠道菌群潜生体大量生长,并可稳定传代,有明显的群集现象。结论:CO_2气腹后肠道细菌繁殖能力及适应能力显著增强,应充分估计其对抗生素的耐受特性。 展开更多
关键词 CO2气腹 肠道菌群 生物学特性 实验研究 细菌显微培养 PCR 细菌形态学
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Bioleaching of complex polymetallic sulfide ores by mixed culture 被引量:3
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作者 王军 赵红波 +1 位作者 覃文庆 邱冠周 《Journal of Central South University》 SCIE EI CAS 2014年第7期2633-2637,共5页
Bacterial leaching of single sulfide minerals and polymetallic sulfide ores was operated in shake flasks and small-scaled columns.The results show that bioleaching of jamesonite is not accessible,the iron extraction r... Bacterial leaching of single sulfide minerals and polymetallic sulfide ores was operated in shake flasks and small-scaled columns.The results show that bioleaching of jamesonite is not accessible,the iron extraction rate of pyrrhotite bioleaching reaches 98.2% after 26 d,and the zinc extraction rate of marmatite bioleaching reaches 92.3%,while the corresponding iron extraction reaches only 13.6% after 29 d.Pulp density has a significant effect on metal extraction of pyrrhotite and marmatite bioleaching.The corresponding metal extraction rate decreases with the increase of pulp density.For the polymetallic sulfide ores,zinc extraction of 97.1% is achieved after bioleaching in shake flasks for 10 d,while only 7.8% is obtained after bioleaching in small-scaled column.Analytical results of scanning electron microscopy(SEM) and energy dispersive X-ray analysis(EDX) reveal that large amount of calcium sulfate is formed on the mineral surface. 展开更多
关键词 polymetallic sulfide ores JAMESONITE MARMATITE PYRRHOTITE BIOLEACHING
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