We studied the phagotrophic ability of dinoflagellate strain Karenia mikimotoi KM-Lu （isolated from the South China Sea）, using fluorescent microspheres, bacteria isolated from the culture of K. mikimotoi and a mari...We studied the phagotrophic ability of dinoflagellate strain Karenia mikimotoi KM-Lu （isolated from the South China Sea）, using fluorescent microspheres, bacteria isolated from the culture of K. mikimotoi and a marine microalgae lsochrysis galbana. We found that K. mikimotoi cultured under conditions of high light intensity could ingest fluorescent microspheres （diameters 0.5 and 2.0 lain） and fluorescence-labeled bacteria and microalgae. Under a low light intensity, however, only fluorescent microspheres （diameter 0.5 μm） and fluorescence-labeled microalgae were ingested. K. mikimotoi showed better growth by ingesting living marine bacteria or microalgae I. galbana than the controls, either in nutrient-depleted or nutrient-replete conditions. In nutrient-depleted conditions, the growth ofK. mikirnotoi was more significant with L galbana as the prey item. In conclusion, the harmful dinoflagellate K. mikimotoi from the South China Sea has apparent phagotrophic ability, and some marine bacteria and microalgae may promote the growth of K. mikimotoi.展开更多
目的建立22q11微缺失综合征的产前诊断方法。方法应用细菌人工染色体标记一磁珠鉴别／分离技术（BACs-on-Beads^TM，BoBs）和荧光原位杂交技术（fluorescence in situ hybridization，FISH），对1例羊水染色体培养失败的胎儿及1例疑似22...目的建立22q11微缺失综合征的产前诊断方法。方法应用细菌人工染色体标记一磁珠鉴别／分离技术（BACs-on-Beads^TM，BoBs）和荧光原位杂交技术（fluorescence in situ hybridization，FISH），对1例羊水染色体培养失败的胎儿及1例疑似22q11微缺失综合征的双胎行产前分子诊断。结果产前BoBs试剂盒方法检测到1例胎儿及1例双胎均为22q11的微缺失，同时3例胎儿中期分裂细胞的FISH验证结果均显示为22q11微缺失，即在DiGeorge／VCFSN25位点上仅有一个红色荧光信号，对照22号末端22q13．3ARSA位点上有两个绿色荧光信号。结论产前Bobs方法联合FISH技术可以成为22q11微缺失的一种产前分子诊断手段。展开更多
基金Supported by the National Natural Science Foundation of China (Nos. 40606029, 40821004)the National Basic Research Program of China (973 Program) (No. 2010CB428705)a project of the Chinese Academy of Sciences (No. KZCX2-YW-Q07-04)
文摘We studied the phagotrophic ability of dinoflagellate strain Karenia mikimotoi KM-Lu （isolated from the South China Sea）, using fluorescent microspheres, bacteria isolated from the culture of K. mikimotoi and a marine microalgae lsochrysis galbana. We found that K. mikimotoi cultured under conditions of high light intensity could ingest fluorescent microspheres （diameters 0.5 and 2.0 lain） and fluorescence-labeled bacteria and microalgae. Under a low light intensity, however, only fluorescent microspheres （diameter 0.5 μm） and fluorescence-labeled microalgae were ingested. K. mikimotoi showed better growth by ingesting living marine bacteria or microalgae I. galbana than the controls, either in nutrient-depleted or nutrient-replete conditions. In nutrient-depleted conditions, the growth ofK. mikirnotoi was more significant with L galbana as the prey item. In conclusion, the harmful dinoflagellate K. mikimotoi from the South China Sea has apparent phagotrophic ability, and some marine bacteria and microalgae may promote the growth of K. mikimotoi.
文摘目的建立22q11微缺失综合征的产前诊断方法。方法应用细菌人工染色体标记一磁珠鉴别／分离技术（BACs-on-Beads^TM，BoBs）和荧光原位杂交技术（fluorescence in situ hybridization，FISH），对1例羊水染色体培养失败的胎儿及1例疑似22q11微缺失综合征的双胎行产前分子诊断。结果产前BoBs试剂盒方法检测到1例胎儿及1例双胎均为22q11的微缺失，同时3例胎儿中期分裂细胞的FISH验证结果均显示为22q11微缺失，即在DiGeorge／VCFSN25位点上仅有一个红色荧光信号，对照22号末端22q13．3ARSA位点上有两个绿色荧光信号。结论产前Bobs方法联合FISH技术可以成为22q11微缺失的一种产前分子诊断手段。
文摘目的产前诊断1例超声异常的Miller-Dieker（Miller—Dieker syndrome，MDS）胎儿，分析并探讨其基因型与表型的对应关系。方法联合应用染色体核型分析、细菌人工染色体标记一磁珠鉴别／分离技术（BACs—on-Beads，BoBs）、荧光原位杂交技术（fluorescence in situ hybridization，FISH）和单核苷酸多态性微阵列技术对1例超声异常的胎儿进行产前诊断。结果产前BoBs检测提示胎儿携带17p13区的MDS微缺失，中期分裂细胞FISH确认其为17p13区的微缺失，高分辨的单核苷酸多态微阵列检测确定该胎儿染色体在17p13区存在约5．2Mb的缺失：arr[hg19]17p13．3p13．2（525—5204373）×1。胎儿脐血细胞染色体核型分析、孕妇及其配偶的外周血高分辨染色体核型分析和BoBs检测均未见异常。结论联合应用产前分子遗传学技术对1例新发的MDS综合征胎儿进行了产前诊断，临床上应重视微缺失微重复的病例，避免漏诊。
