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细菌微量快速生化鉴定方法学研究 被引量:4
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作者 赵院利 任健康 闫福堂 《现代检验医学杂志》 CAS 2006年第4期53-54,共2页
目的 建立细菌微量快速生化鉴定系统,用于临床实验室细菌学的快速诊断。方法 应用所研制的27种微量快速生化反应试剂与传统试管方法检测208株临床分离菌及8种质控菌株生化反应符合率,依据杭州24h的JYZ-15E及JYZ—11E肠杆菌生化编码鉴... 目的 建立细菌微量快速生化鉴定系统,用于临床实验室细菌学的快速诊断。方法 应用所研制的27种微量快速生化反应试剂与传统试管方法检测208株临床分离菌及8种质控菌株生化反应符合率,依据杭州24h的JYZ-15E及JYZ—11E肠杆菌生化编码鉴定管的项目要求组合了该法的肠杆菌15种(RE15n)及肠杆菌11种(RE11n)生化鉴定试验条,用208株临床分离菌及8种质控菌菌株进行鉴定试验。结果 27种细菌微量快速生化反应试剂与传统试管法生化反应总符合率为93.5%~100%。对208株临床分离菌及8株质控菌的菌种鉴定,其完全符合率15种(RE15n)为99.9%,11种(RE11n)为100%。8种质控菌株鉴定完全正确。结论 用细菌微量快速生化反应系统鉴定细菌,具有简单、快速的特点,无需特殊设备,成本低廉,所得结果准确、可靠,能够满足临床需要。 展开更多
关键词 微量 快速 细菌生化反应 方法研究
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肠杆菌科细菌糖快速生化反应实验探讨 被引量:1
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作者 姜杭华 吴振安 《中国卫生检验杂志》 2000年第3期360-361,共2页
关键词 肠杆菌科 细菌糖快速生化反应 临床检验
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Isolation and Characteristics of New Heterotrophic Nitrifying Bacteria 被引量:1
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作者 苏俊峰 马放 +4 位作者 王弘宇 郭静波 候宁 李维国 魏利 《Journal of Donghua University(English Edition)》 EI CAS 2007年第4期546-549,553,共5页
The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. When influent Ammonia nitrogen concentration was 42. 78 - 73. 62 mg/L. The average amm... The study presented the method for isolating the heterotrophic nitrifiers and the characterization of heterotrophic nitrification. When influent Ammonia nitrogen concentration was 42. 78 - 73. 62 mg/L. The average ammonia nitrogen removal rate was 81.32% from the bioceramics reactor. Sodium acetate and ammonium chloride were used as carbon and nitrogen source. The COD removal rates by microorganisms of strain wgy21 and wgy36 were 56.1% and 45.45%, respectively. The TN removal rates by microorganisms of strain wgy21 and wgy36 were 65.85% and 67. 98%, respectively. At the same time, the concentration of ammonium nitrogen was with the removal rates of 75.25% and 84.96%, and it also had the function of producing NO2 - N. Sodium acetate and sodium nitrite were used as carbon and nitrogen source. Through the 12 days of the aerobic culture, the COD femoral rates by microorganisms of strain wgy21 and wgy36 were 29.25% and 22.08%, respectively. NO2 -N concentration decreased slowly. Comparison, similarity of wgy21 and many Acinetobacter sp. ≥99%, similarity of wgy36 and many Acinetobacter sp. ≥99%. Refer to routine physiologicalbiochemical characteristic determination, further evidences showed that wgy21 and wgy36 belong to Acinetobacter sp., respectively. 展开更多
关键词 bio-ceramic reactor isolation and identification heterotrophic nitrification nitrifying characteristic
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