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基于CadR的镉生物传感器的构建与优化
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作者 蔡叶申 张天一 +2 位作者 鲍凌志 马洁 陈少鹏 《环境工程技术学报》 CAS CSCD 北大核心 2023年第2期873-880,共8页
以镉调控蛋白(cadmium resistance protein,CadR)为检测元件、绿色荧光蛋白为报告元件构建镉离子细菌生物传感器检测水体样品中的重金属镉,通过单因素试验和正交试验对检测条件进行优化以提升传感器的相对荧光强度,基于最优组合使用所... 以镉调控蛋白(cadmium resistance protein,CadR)为检测元件、绿色荧光蛋白为报告元件构建镉离子细菌生物传感器检测水体样品中的重金属镉,通过单因素试验和正交试验对检测条件进行优化以提升传感器的相对荧光强度,基于最优组合使用所构建的镉细菌生物传感器对加标长江水样进行检测。结果表明:所构建的重金属镉细菌生物传感器在0~200μg/L浓度范围内对镉的荧光响应符合剂量依赖效应;单因素试验结果表明,温度、pH、诱导时间以及阳离子对镉生物传感器的灵敏度有显著影响,而通过改变不同阴离子配体对检测结果并未产生显著影响;正交试验优化结果显示,镉细菌生物传感器的最优检测条件组合为:温度为37℃,pH为7,诱导时间为120 min,添加终浓度为50μg/L的Mg^(2+),在此条件下传感器响应镉的相对荧光强度相较于优化前增强了1.6倍;使用该传感器对加标长江水样检测结果显示,其对于0~500μg/L的环境水样中的镉具有较好的检测能力,荧光响应最大值达到背景值的20倍。 展开更多
关键词 重金属 细菌生物传感器 镉调控蛋白 性能优化
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Bacteriorhodopsin and SWCNT Scaffold for Optical Nanobiosensor
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作者 Aiswarya Radhakrishna Pillai Balraj Arunachalam +1 位作者 Manish Shinde Rabinder Henry 《Journal of Life Sciences》 2010年第6期60-64,共5页
This paper describes theoretical steps to develop an optical nanobiosensor using bacteriorhodopsin (BR) as the biomembrane and Single-Walled Carbon NanoTube (SWCNT) as the scaffold. Bacteriorhodopsin is a retinal ... This paper describes theoretical steps to develop an optical nanobiosensor using bacteriorhodopsin (BR) as the biomembrane and Single-Walled Carbon NanoTube (SWCNT) as the scaffold. Bacteriorhodopsin is a retinal protein used by archaea that come under the family of halobacteria. This retinal protein acts as a proton pump and resulting proton gradient is used to change the voltage that pass across the drain and source. The biosensor contains nano ISFET where the channel is made of a carbon nanotube for the conduction of current. The gate is replaced by bacteriorhodopsin biomembrane. Bacteriorhodopsin can be used as a molecular-level ultra fast bi-stable red / green photo switch for making 3D optical molecular memories that reliably store data with 10,000 molecules/bit. The molecules switch in femtoseconds. Biomembrane will sense 510 nm and 650 nm wavelength of light and the sensing voltage can be used to convert the data into digital signals. This molecular level memory device can be used for ‘Read-Write' operations. The sensor performance will also be ultra fast since it uses photons for the data storage, which are much faster than electrons used in normal memory devices, and the 3D storage capacity is much higher maximum of 10^13/cm^2. 展开更多
关键词 Optical nanobiosensor BR HALOBACTERIA nano ISFET (Ion-Sensitive Field-Effect Transistor) archaea.
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