环境污染物苯并(a)芘(简称 BP)是一种潜在的致癌物质。近年来已经认识到,BP 在生物体内经历着脱毒代谢和活化代谢两个方向的代谢过程,即在细胞肉质网中的芳烃环氧化酶(AE)、环氧化物水化酶(EH)和有关结合酶的作用下,生成 BP 的酚、醌、...环境污染物苯并(a)芘(简称 BP)是一种潜在的致癌物质。近年来已经认识到,BP 在生物体内经历着脱毒代谢和活化代谢两个方向的代谢过程,即在细胞肉质网中的芳烃环氧化酶(AE)、环氧化物水化酶(EH)和有关结合酶的作用下,生成 BP 的酚、醌、二氢二醇和相应缀合物。近致癌物二氢二醇则可进一步活化代谢,形成强亲电性的终致癌物四氢二醇环氧化物与 DNA 等生物大分子共价结合。BP 代谢产物的分离和鉴定。展开更多
Cytochrome P4501A1 (CYP1A1) plays an important role in the bioactivation processes that transform aromatic hydrocarbons into ultimate carcinogens. Genetically determined differences in activity of this enzyme could mo...Cytochrome P4501A1 (CYP1A1) plays an important role in the bioactivation processes that transform aromatic hydrocarbons into ultimate carcinogens. Genetically determined differences in activity of this enzyme could modulate individual susceptibility to develop cancers. The role of CYP1A1 in metabolic pathway of estrogens suggests an influence on carcinogenic events in genital tissues. The aim of our study was to elucidate the possible role of CYP1A1 alleles in the pathogenesis of endometrial and ovarian cancers. We have compared CYP1A1 genotype frequency between genital (endometrial and ovarian) cancer groups and 212 healthy women. Cancer patients were stratified using FIGO classification and diagnoses were confirmed histopathologically. The analysis of CYP1A1 genotypes was performed using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) assay. We have observed higher frequency of heterozygotic genotypes containing mutation m4 (CYP1A1* 1/* 4) in cancer groups (5.1% in ovarian and 5.6% in endometrial cancer versus 1.9% in controls). The higher frequency of mutated CYP1A1* 4 allele connected with lower frequency of CYP1A1* 2A and CYP1A1* 2B in endometrial and ovarian cancer groups indicates that differences in the metabolic activity of CYP1A1 could play a significant role in the pathogenesis of genital cancers.展开更多
文摘环境污染物苯并(a)芘(简称 BP)是一种潜在的致癌物质。近年来已经认识到,BP 在生物体内经历着脱毒代谢和活化代谢两个方向的代谢过程,即在细胞肉质网中的芳烃环氧化酶(AE)、环氧化物水化酶(EH)和有关结合酶的作用下,生成 BP 的酚、醌、二氢二醇和相应缀合物。近致癌物二氢二醇则可进一步活化代谢,形成强亲电性的终致癌物四氢二醇环氧化物与 DNA 等生物大分子共价结合。BP 代谢产物的分离和鉴定。
文摘Cytochrome P4501A1 (CYP1A1) plays an important role in the bioactivation processes that transform aromatic hydrocarbons into ultimate carcinogens. Genetically determined differences in activity of this enzyme could modulate individual susceptibility to develop cancers. The role of CYP1A1 in metabolic pathway of estrogens suggests an influence on carcinogenic events in genital tissues. The aim of our study was to elucidate the possible role of CYP1A1 alleles in the pathogenesis of endometrial and ovarian cancers. We have compared CYP1A1 genotype frequency between genital (endometrial and ovarian) cancer groups and 212 healthy women. Cancer patients were stratified using FIGO classification and diagnoses were confirmed histopathologically. The analysis of CYP1A1 genotypes was performed using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) assay. We have observed higher frequency of heterozygotic genotypes containing mutation m4 (CYP1A1* 1/* 4) in cancer groups (5.1% in ovarian and 5.6% in endometrial cancer versus 1.9% in controls). The higher frequency of mutated CYP1A1* 4 allele connected with lower frequency of CYP1A1* 2A and CYP1A1* 2B in endometrial and ovarian cancer groups indicates that differences in the metabolic activity of CYP1A1 could play a significant role in the pathogenesis of genital cancers.
