Objective To observe the clinical curative effect of eye acupuncture therapy on primary dysmenorrhea and the effect on content of prostaglandin F2α （PGF2α） in peripheral blood of menstrual periods. Methods One hun...Objective To observe the clinical curative effect of eye acupuncture therapy on primary dysmenorrhea and the effect on content of prostaglandin F2α （PGF2α） in peripheral blood of menstrual periods. Methods One hundred and ten cases of primary dysmenorrhea were randomly divided into an eye acupuncture group （60 cases） and a medication group （50 cases）. In the eye acupuncture group, lower-jiao area, liver area, kidney area, heart area and spleen area, etc. were selected according to syndrome differentiation, and the eye acupuncture therapy was applied. In the medication group, 300 mg Ibuprofen sustained release capsule was orally administrated 1–2 days before menstrual onset or when the symptoms appeared, once every 12 hours, for 3–5 days totally, 3 menstrual periods constituting one therapeutic course. The content of PGF2α in peripheral blood, and the curative effects and recurrence 3 and 6 months after treatment were observed. Results Three months after treatment, the cured rate was 55.0% （33/60） and the total effective rate was 95.0% （57/60） in the eye acupuncture group, which were superior to those of 34.0% （17/50） and 82.0% （41/50） in the medication group （both P0.05）. According to the following-up after 6 menstrual cycles, the recurrence rate of 9.1% （3/33） in the acupuncture group was significantly lower than that of 35.3% （6/11） in medication group. The contents of PGF2α in peripheral blood of menstrual periods were reduced after treatment in the two groups （both P0.01）. Conclusion The curative effect of eye acupuncture on primary dysmenorrhea is significant, with the level of PGF2α in peripheral blood of menstrual periods reduced.展开更多
Objective:To observe the effects of electroacupuncture(EA)on uterine prostaglandin F2α(PGF2α),cyclooxygenase 2(COX-2)and nuclear factorκB(NF-κB)in rats with primary dysmenorrhea(PD)and to discuss the possible mech...Objective:To observe the effects of electroacupuncture(EA)on uterine prostaglandin F2α(PGF2α),cyclooxygenase 2(COX-2)and nuclear factorκB(NF-κB)in rats with primary dysmenorrhea(PD)and to discuss the possible mechanism in EA intervening PD.Methods:Forty Sprague-Dawley female rats were randomly divided into a blank group,a model group,an EA group and an ibuprofen group,with 10 rats in each group.The PD model was established using estradiol benzoate combined with oxytocin in the model group,EA group and ibuprofen group.At the same time of modeling,rats in the EA group were given EA at Guanyuan(CV 4)and Sanyinjiao(SP 6)once a day for 20 min each time for 10 consecutive days.Ibuprofen was intragastrically administered once a day for 10 consecutive days in the ibuprofen group.The same amount of normal saline was intragastrically administered once a day for 10 consecutive days in the blank group and model group.The number of writhing of rats in each group within 30 min was compared on the 11th day just after the interventions.The uterine homogenate supernatant was separated and the PGF2αlevel was detected by enzyme-linked immunosorbent assay.Western blot was applied for the detection of the expression levels of COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine tissues.Results:Compared with the blank group,the number of writhing in the model group increased significantly(P<0.01),and the expression levels of PGF2α,COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine tissues were significantly increased(all P<0.01).Compared with the model group,the number of writhing in the EA group and ibuprofen group were significantly reduced(both P<0.01),and the expression levels of PGF2αand COX-2 protein in uterine tissues were significantly reduced(both P<0.01).Compared with the model group,the phospho-NF-κB p65 level in uterine tissues in the EA group was significantly reduced(P<0.01).Compared with the ibuprofen group,the phospho-NF-κB p65 level in the EA group was significantly reduced(P<0.01).Conclusion:The mechanism of EA for PD rats may be related to inhibiting the phosphorylation of NF-κB and reducing the levels of COX-2 and PGF2αin uterine tissues.展开更多
文摘Objective To observe the clinical curative effect of eye acupuncture therapy on primary dysmenorrhea and the effect on content of prostaglandin F2α （PGF2α） in peripheral blood of menstrual periods. Methods One hundred and ten cases of primary dysmenorrhea were randomly divided into an eye acupuncture group （60 cases） and a medication group （50 cases）. In the eye acupuncture group, lower-jiao area, liver area, kidney area, heart area and spleen area, etc. were selected according to syndrome differentiation, and the eye acupuncture therapy was applied. In the medication group, 300 mg Ibuprofen sustained release capsule was orally administrated 1–2 days before menstrual onset or when the symptoms appeared, once every 12 hours, for 3–5 days totally, 3 menstrual periods constituting one therapeutic course. The content of PGF2α in peripheral blood, and the curative effects and recurrence 3 and 6 months after treatment were observed. Results Three months after treatment, the cured rate was 55.0% （33/60） and the total effective rate was 95.0% （57/60） in the eye acupuncture group, which were superior to those of 34.0% （17/50） and 82.0% （41/50） in the medication group （both P0.05）. According to the following-up after 6 menstrual cycles, the recurrence rate of 9.1% （3/33） in the acupuncture group was significantly lower than that of 35.3% （6/11） in medication group. The contents of PGF2α in peripheral blood of menstrual periods were reduced after treatment in the two groups （both P0.01）. Conclusion The curative effect of eye acupuncture on primary dysmenorrhea is significant, with the level of PGF2α in peripheral blood of menstrual periods reduced.
文摘Objective:To observe the effects of electroacupuncture(EA)on uterine prostaglandin F2α(PGF2α),cyclooxygenase 2(COX-2)and nuclear factorκB(NF-κB)in rats with primary dysmenorrhea(PD)and to discuss the possible mechanism in EA intervening PD.Methods:Forty Sprague-Dawley female rats were randomly divided into a blank group,a model group,an EA group and an ibuprofen group,with 10 rats in each group.The PD model was established using estradiol benzoate combined with oxytocin in the model group,EA group and ibuprofen group.At the same time of modeling,rats in the EA group were given EA at Guanyuan(CV 4)and Sanyinjiao(SP 6)once a day for 20 min each time for 10 consecutive days.Ibuprofen was intragastrically administered once a day for 10 consecutive days in the ibuprofen group.The same amount of normal saline was intragastrically administered once a day for 10 consecutive days in the blank group and model group.The number of writhing of rats in each group within 30 min was compared on the 11th day just after the interventions.The uterine homogenate supernatant was separated and the PGF2αlevel was detected by enzyme-linked immunosorbent assay.Western blot was applied for the detection of the expression levels of COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine tissues.Results:Compared with the blank group,the number of writhing in the model group increased significantly(P<0.01),and the expression levels of PGF2α,COX-2,phospho-NF-κB p65 and NF-κB p65 proteins in uterine tissues were significantly increased(all P<0.01).Compared with the model group,the number of writhing in the EA group and ibuprofen group were significantly reduced(both P<0.01),and the expression levels of PGF2αand COX-2 protein in uterine tissues were significantly reduced(both P<0.01).Compared with the model group,the phospho-NF-κB p65 level in uterine tissues in the EA group was significantly reduced(P<0.01).Compared with the ibuprofen group,the phospho-NF-κB p65 level in the EA group was significantly reduced(P<0.01).Conclusion:The mechanism of EA for PD rats may be related to inhibiting the phosphorylation of NF-κB and reducing the levels of COX-2 and PGF2αin uterine tissues.
