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宁心红杞胶囊对围绝经大鼠卵巢局部自分泌及旁分泌因子的调节作用 被引量:3
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作者 谢玲 赖仁胜 +2 位作者 王黎 马红 吕晔 《中国中西医结合杂志》 CAS CSCD 北大核心 2008年第3期242-244,共3页
目的探讨宁心红杞胶囊对围绝经大鼠卵巢局部自分泌及旁分泌因子的调节作用。方法选用4月龄雌性大鼠(正常组),14月龄阴道细胞学表现动情期延长的雌性大鼠(作为老年大鼠模型)。模型大鼠随机分为宁心红杞大、中、小剂量组、阳性药(雌二醇)... 目的探讨宁心红杞胶囊对围绝经大鼠卵巢局部自分泌及旁分泌因子的调节作用。方法选用4月龄雌性大鼠(正常组),14月龄阴道细胞学表现动情期延长的雌性大鼠(作为老年大鼠模型)。模型大鼠随机分为宁心红杞大、中、小剂量组、阳性药(雌二醇)组和模型组,分别给予相应药物。HE染色观察卵巢生长卵泡、黄体等情况;免疫组化检测卵巢雌激素受体(ER)、孕激素受体(PR)、促卵泡激素(FSH)、促黄体激素(LH)、抑制素α(INHα)、激活素(ACT)α-β、卵泡抑制素(FS)、胰岛素样生长因子(IGF)-1的表达。结果与模型组比较,宁心红杞大、中剂量组卵巢指数、生长卵泡数增加(P<0.05),FSH、LH水平表达降低(P<0.05);宁心红杞大剂量组和阳性药物组ER表达水平上升(P<0.05),宁心红杞大、中剂量组IGF-1和INHα表达水平升高(P<0.05),而ACTα-β和FS表达水平下降(P<0.05)。结论宁心红杞胶囊可使卵巢局部自分泌、旁分泌系统调节因子相互协调平衡,进而改善卵巢功能。 展开更多
关键词 宁心红杞胶囊 围绝经大鼠 自分泌 旁分泌
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宁心红杞胶囊对围绝经大鼠卵巢颗粒细胞凋亡及Bcl-2和Bax表达的影响 被引量:1
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作者 谢玲 赖仁胜 +2 位作者 朱长乐 马红 吕晔 《中药药理与临床》 CAS CSCD 北大核心 2009年第4期58-60,共3页
目的:探讨宁心红杞胶囊对围绝经大鼠卵巢颗粒细胞凋亡及其相关基因Bax和Bc l-2表达的影响。方法:选用4月龄雌性大鼠为正常对照组,14月龄阴道细胞学表现动情期延长的雌性大鼠作为衰老大鼠模型。模型动物随机分别给宁心红杞胶囊或补佳乐... 目的:探讨宁心红杞胶囊对围绝经大鼠卵巢颗粒细胞凋亡及其相关基因Bax和Bc l-2表达的影响。方法:选用4月龄雌性大鼠为正常对照组,14月龄阴道细胞学表现动情期延长的雌性大鼠作为衰老大鼠模型。模型动物随机分别给宁心红杞胶囊或补佳乐治疗。采用原位末端核苷酸标记法(TUNEL)检测卵巢细胞凋亡,免疫组化法检测凋亡相关因子Bc l-2、Bax。结果:宁心红杞胶囊高、中剂量组卵巢细胞凋亡阳性表达与模型组比较有显著性差异,同时可使卵巢抑凋亡基因Bc l-2表达增强,促凋亡基因Bax表达减弱,Bc l-2/Bax比例增加。结论:宁心红杞胶囊通过抑制Bax蛋白的表达,上调Bc l-2蛋白表达,延缓卵巢颗粒细胞凋亡,进而改善卵巢功能。 展开更多
关键词 宁心红杞胶囊 围绝经大鼠 细胞凋亡
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养肝解郁颗粒对绝经后大鼠非酒精性单纯性脂肪肝脂代谢及肝细胞脂肪变性的影响
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作者 丁云录 杨明 +4 位作者 申巧慧 李驰坤 李晓兵 欧喜燕 冷炎 《吉林中医药》 2023年第2期194-198,共5页
目的通过实验研究验证养肝解郁颗粒对绝经后大鼠非酒精性单纯性脂肪肝脂代谢及肝细胞脂肪变性的影响。方法采用大鼠去氧乙烯基环乙烯腹腔注射法来建立绝经期动物模型,通过饲喂高脂高糖饲料制备非酒精性单纯性脂肪肝模型,诱导建立绝经期... 目的通过实验研究验证养肝解郁颗粒对绝经后大鼠非酒精性单纯性脂肪肝脂代谢及肝细胞脂肪变性的影响。方法采用大鼠去氧乙烯基环乙烯腹腔注射法来建立绝经期动物模型,通过饲喂高脂高糖饲料制备非酒精性单纯性脂肪肝模型,诱导建立绝经期非酒精性单纯性脂肪肝大鼠动物模型。预防性给药12周后,检测血清AST、ALT、TC、TG水平,计算肝系数,检测肝组织中TC、TG、MDA、SOD、FFA含量,肝脏病理组织学检查。结果养肝解郁颗粒6.0、3.0、1.5 g/kg给药组血清及肝组织匀浆中TC、TG含量、血清AST活性均明显低于模型组(为P<0.01或P<0.05);6.0、3.0 g/kg给药组大鼠血清ALT、肝组织匀浆中MDA、FFA含量、肝脏系数均明显低于模型组(为P<0.01或P<0.05);1.5 g/kg给药组大鼠肝组织匀浆中MDA含量低于模型对照组(为P<0.05);6.0 g/kg给药组大鼠肝组织匀浆中SOD活性明显高于模型对照组(为P<0.05)。养肝解郁颗粒3个剂量均可不同程度减轻肝脏病理损伤。结论养肝解郁颗粒可以调节绝经后大鼠非酒精性单纯性脂肪肝脂代谢、减轻肝细胞脂肪变性程度,从而达到治疗非酒精性单纯性脂肪肝的目的。 展开更多
关键词 非酒精性单纯性脂肪肝 养肝解郁颗粒 经大鼠 脂代谢 肝细胞脂肪变性
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人脐带间充质干细胞移植对围绝经期大鼠性激素的影响 被引量:2
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作者 曾禄贤 胡佳佳 尹春艳 《广东医学》 CAS 北大核心 2016年第8期1140-1142,共3页
目的探讨人脐带间充质干细胞移植对围绝经期大鼠性激素水平的影响。方法阴道脱落细胞涂片法筛选出30只月龄13-15个月的SPF级SD围绝经期大鼠模型,随机分为A、B、C 3组(每组10只),喂养方式相同。A组不予处理,B组经尾静脉移植浓度为1... 目的探讨人脐带间充质干细胞移植对围绝经期大鼠性激素水平的影响。方法阴道脱落细胞涂片法筛选出30只月龄13-15个月的SPF级SD围绝经期大鼠模型,随机分为A、B、C 3组(每组10只),喂养方式相同。A组不予处理,B组经尾静脉移植浓度为1×10^6cell/m L的人脐带间充质干细胞1 m L,隔日1次,共2次;C组与B组同一方法和时间移植PBS液1 m L。于成模时和末次移植后7和14 d检测各组大鼠性激素:雌二醇(E2)、孕酮(P)、黄体生成素(LH)、卵泡刺激素(FSH)的水平。结果 3组大鼠成模时及末次移植后7 d E2、P、FSH、LH水平比较差异无统计学意义(P〉0.05);B组大鼠E2、P水平随移植时间延长呈上升趋势,FSH水平随移植时间延长呈下降趋势;末次移植后14 d E2、FSH、P水平与末次移植后7 d及移植前比较差异有统计学意义(P〈0.05)。B组末次移植后14 d与A组和C组相同实验时间段E2、P、FSH、LH水平比较差异有统计学意义(P〈0.05);A组和C组不同实验时间段E2、P水平呈下降趋势,FSH、LH水平呈上升趋势,移植前与移植14 d后比较差异有统计学意义(P〈0.05)。结论人脐带间充质干细胞移植能改善围绝经期大鼠性激素水平,提高卵巢储备能力。 展开更多
关键词 人脐带间充质干细胞 围绝经大鼠 性激素
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全身振动训练对大鼠骨骼肌的改善及其作用机制研究 被引量:4
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作者 张轲 罗由平 《中国骨质疏松杂志》 CAS CSCD 北大核心 2018年第5期619-624,共6页
目的通过观察全身振动性训练法(WBV)对去卵巢(OVX)大鼠骨骼肌中过氧化物酶体增殖剂激活受体(PPARγ),β-连环蛋白和磷酸化糖原的表达合成酶激酶-3(P-GSK-3β)蛋白质表达的影响,探讨WBV对绝经大鼠骨骼肌的作用机制。方法将雌性大鼠随机... 目的通过观察全身振动性训练法(WBV)对去卵巢(OVX)大鼠骨骼肌中过氧化物酶体增殖剂激活受体(PPARγ),β-连环蛋白和磷酸化糖原的表达合成酶激酶-3(P-GSK-3β)蛋白质表达的影响,探讨WBV对绝经大鼠骨骼肌的作用机制。方法将雌性大鼠随机分成正常对照组(Sham组,n=10),去卵巢组(OVX组,n=10),17β-雌二醇治疗组(E_2组,n=10)和全身振动性训练组(WBV组,n=10)。测定各组大鼠血清E_2和黄体生成素(LH)水平,以及PPARγ、β-连环蛋白和P-GSK-3β在骨骼肌中的表达。结果 OVX组大鼠血清LH比Sham组和E_2组明显升高(P<0.05)。OVX组大鼠血清E_2水平与Sham组、E_2组和WBV组相比明显上升(P<0.05)。OVX组骨骼肌PPARγ表达较Sham组显著升高(P<0.05),而β-连环蛋白和P-GSK-3β蛋白表达较Sham组均显著降低(P<0.05)。在WBV或雌激素替代治疗的干预下,WBV组或E_2组的PPARγ表达均显著低于OVX组(P<0.05),β-连环蛋白和P-GSK-3β蛋白表达均显著高于OVX组(P<0.05)。结论 WBV能有效预防OVX大鼠的骨质流失,并提高骨质强度,这可能与抑制OVX大鼠骨骼肌中GSK-3β和PPARγ活性来激活β-连环蛋白信号传导通路有关。 展开更多
关键词 全身振动训练 经大鼠 骨骼肌 Β-连环蛋白 GSK-3Β PPARΓ
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大鼠急性心肌梗死模型制作方法的改良 被引量:3
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作者 吴庆景 高凤敏 《牡丹江医学院学报》 2017年第2期22-24,共3页
目的改良大鼠急性心肌梗死模型制作方法,提高模型成功率。方法 40只大鼠随机分组为乌拉坦组(A组)、乌拉坦联合利多卡因组(B组)、戊巴比妥钠组(C组)和戊巴比妥钠联合利多卡因组(D组),戊巴比妥钠和乌拉坦常规剂量,联合利多卡因组加2%利多... 目的改良大鼠急性心肌梗死模型制作方法,提高模型成功率。方法 40只大鼠随机分组为乌拉坦组(A组)、乌拉坦联合利多卡因组(B组)、戊巴比妥钠组(C组)和戊巴比妥钠联合利多卡因组(D组),戊巴比妥钠和乌拉坦常规剂量,联合利多卡因组加2%利多卡因0.1ml/200g,行腹腔麻醉,记录各组麻醉时间、术中恶性心律失常发生率;麻醉后每组大鼠经口气管插管,开胸结扎冠状动脉前降支,记录大鼠死亡率及手术时间;术后48h随机取各组大鼠5只用Evans Blue和TTC染色检测心肌梗死面积。结果麻醉时间A组(18.7±5.2h)和B组(18.2±6.1h)明显长于C组(3.7±1.3h)和D组(3.8±1.2h)(P<0.05),A组和B组麻醉时间及C组和D组麻醉时间差别均无统计学意义(P>0.05);恶性心律失常发生率A组(20%)和C组(20%)明显高于B组(0%)和D组(0%)(P<0.05),A组和C组及B组和D组恶性心律失常发生率差别均无统计学意义(P>0.05);死亡率A组(40%)和B组(40%)明显高于C组(20%)和D组(0%)(P<0.05),A组和B组及C组和D组死亡率差别有统计学意义(P<0.05),D组死亡率明显低于其他各组(P<0.05);A组、B组、C组、D组心肌梗死面积分别为72±4%、73±4%、73±2%、73±3%差异均无统计学意义(P>0.05)。结论使用戊巴比妥钠联合利多卡因是较好的麻醉方法,改良后模型制作方法单人可独立完成、模型质量较高,值得推广。 展开更多
关键词 经大鼠 急性心肌梗死 模型 制作.
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Identification of differentially expressed genes in dorsal root ganglion in early diabetic rats
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作者 朱清 顾锦华 +1 位作者 朱红艳 徐济良 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第4期219-224,共6页
Objective To screen and identify differentially expressed genes in the dorsal root ganglion (DRG) in early experimental diabetic rats. Methods Diabetic model rats were induced by single intraperitoneal injection of ... Objective To screen and identify differentially expressed genes in the dorsal root ganglion (DRG) in early experimental diabetic rats. Methods Diabetic model rats were induced by single intraperitoneal injection of streptozotocin (STZ). At the second week after STZ injection, the sensory nerve conduction velocities (SNCV) of sciatic nerve were measured as an indicator of neuropathy. The technique of silver-staining mRNA differential display polymerase chain reaction (DD-PCR) was used to detect the levels of differentially expressed genes in rat DRG. The cDNA fragments that displayed differentially were identified by reverse-hybridization, cloned and sequenced subsequently, and then confirmed by Northern blot. Results The SNCV in the diabetic model group [n = 9, (45.25±10.38) m/s] reduced obviously compared with the control group [n = 8, (60.10± 11.92) m/s] (P 〈 0.05). Seven distinct cDNA clones, one was up-regulated gene and the others were downregulated ones, were isolated by silver-staining mRNA differential display method and confirmed by Northern blot. According to the results of sequence alignment with GenBank data, majority of the clones had no significant sequence similarity to previously reported genes except only one that showed high homology to 6-pyruvoyl-tetrahydropterin synthase mRNA (accession No., BC059140), which had not been reported to relate to diabetic neuropathy. Conclusion These differentially expressed genes in the diabetic DRG may contribute to the pathogenesis of diabetic peripheral neuropathy. 展开更多
关键词 differential display polymerase chain reaction silver staining MRNA dorsal root ganglion DIABETES RAT
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Urocortin, the neuropeptide, inhibits the viability of ECV304 cells and rat vascular smooth muscle cells
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作者 陈洁 汪红仪 +3 位作者 陶金 徐华娥 杨荣 李胜男 《Journal of Nanjing Medical University》 2004年第1期1-3,共3页
Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' t... Objective: This study aims to investigate the effects of urocortin (Ucn) on the viability of endothelial cells (ECV304) and rat vascular muscle cells (VSMC). Methods: Rat aortic VSMC were isolated from the rats' thoracic aorta. We studied the effect of Ucn on the viability of ECV304 cells and VSMC by using a tetrazolium (MTT) assay.Results: Ucn (10 -7 mol/L) inhibited the viability of ECV304 cells and VSMC. Inhibition rates are 13% and 15%, respectively(P<0.05, compared with Control). This inhibition was not dependent on the affecting time and was not affected by the addition of ATP-sensitive potassium channel (KATP channel) blocker, glybenclamide (Gly, 10 mol/L). Conclusion: Ucn inhibits the viability of ECV304 and VSMC. Our results suggest that Ucn may be a new vasoactive agent and may have a beneficial effect in the process of vascular remodeling (VR). 展开更多
关键词 UROCORTIN ECV304 vascular smooth muscle cells MTT assay ATP-sensitive potassium channels
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Neuroprotective action of Ginkgo biloba on the enteric nervous system of diabetic rats 被引量:8
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作者 Glasiella Gonzalez Perez da Silva Jacqueline Nelisis Zanoni Nilza Cristina Buttow 《World Journal of Gastroenterology》 SCIE CAS CSCD 2011年第7期898-905,共8页
AIM: To investigate the effect of Ginkgo biloba extract on the enteric neurons in the small intestine of diabetic rats. METHODS: Fifteen Wistar rats were divided into three groups: control group (C), diabetic group (D... AIM: To investigate the effect of Ginkgo biloba extract on the enteric neurons in the small intestine of diabetic rats. METHODS: Fifteen Wistar rats were divided into three groups: control group (C), diabetic group (D) and diabetic-treated (DT) daily with EGb 761 extract (50 mg/kg body weight) for 120 d. The enteric neurons were identified by the myosin-V immunohistochemical technique. The neuronal density and the cell body area were also analyzed. RESULTS: There was a significant decrease in the neuronal population (myenteric plexus P = 0.0351; submucous plexus P = 0.0217) in both plexuses of the jejunum in group D when compared to group C. With regard to the ileum, there was a significant decrease (P = 0.0117) only in the myenteric plexus. The DT group showed preservation of the neuronal population in the jejunum submucous plexus and in the myenteric plexus in the ileum. The cell body area in group D increased significantly (P = 0.0001) in the myenteric plexus of both segments studied as well as in the ileum submucosal plexus, when compared to C. The treatment reduced (P = 0.0001) the cell body area of the submucosal neurons of both segments and the jejunum myenteric neurons. CONCLUSION: The purified Ginkgo biloba extract has a neuroprotective effect on the jejunum submucous plexus and the myenteric plexus of the ileum of diabetic rats. 展开更多
关键词 Diabetes mellitus Ginkgo biloba Myenteric plexus Submucous plexus NEUROPROTECTION
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Efficacy of Antisense Oligodeoxynucleotide of Neuropeptide Y Y5 Receptor on Treating of Hyperleptinemia by Intraventricular Administration in Diet-induced Obese Rats
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作者 王玢 郭锡熔 +3 位作者 龚海霞 陈荣华 刘倩琦 费莉 《Journal of Nanjing Medical University》 2004年第1期11-15,共5页
Objective: To study the efficacy of antisense oligonucleotide of neuropeptide Y (NPY) Y5 receptor on treating hyperleptinemia by intracerebral ventricular administration in diet-induced obese rats.Methods: The obese r... Objective: To study the efficacy of antisense oligonucleotide of neuropeptide Y (NPY) Y5 receptor on treating hyperleptinemia by intracerebral ventricular administration in diet-induced obese rats.Methods: The obese rats were prepared by feeding a high-nutritive diet for 7 weeks. The lateral ventricle of obese rats was cannulated. Either 10 μl of different neuropeptide Y Y5 receptor oligodeoxynucleotide, including antisense, sense and missense oligodeoxynucleotide (5 g/L) or 10 μl saline was administered into the ventricle through cannula three times per day in every rat. Two days later the rats were slaughtered .The weights of both retroperitoneal and epididymal adipose tissues were measured, and the serum insulin and leptin were detected by radioimmunoassay method and the murine leptin ELISA kit respectively. Results: ①The level of serum was significantly higher in experimental rats than that in normal rats. Similarly, the level of serum insulin and the weights of both retroperitoneal and epididymal adipose tissues were increased in experimental rats. ②After the diet-induced obese rats were intraventricularly administered with NPY Y5 receptor antisense oligodeoxynucleotide, the levels of serum leptin and insulin were significantly decreased and combined with the reduction of weight in retroperitoneal adipose tissue. There was, however, no significant difference in the weight of epidymal adipose tissue between pre-treated and post-treated duration. ③There was significant positive correlation among the level of serum leptin, the level of serum insulin and the weight of retroperritoneal adipose tissue in diet-induced obese rats. Conclusion: Intracerebral ventricular administration of antisense oligodeoxynucleotide of neuropeptide Y Y5 receptor may alleviate hyperleptinemia in diet-induced obese rats and decrease the weight of retroperitoneal adipose tissue and the level of serum insulin. 展开更多
关键词 receptors neuropeptide Y gene therapy hyperleptinemia obesity rats
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Evoked membrane potential change in rat optic nerve fiber:Computer simulation
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作者 万生 乔清理 +1 位作者 李丽明 任秋实 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第6期348-356,共9页
Objective The optic nerve is a key component regarding research on visual prosthesis.Previous pharmacological and electrical studies has pinned down the main features of the mechanisms underlying the nerve impulse in ... Objective The optic nerve is a key component regarding research on visual prosthesis.Previous pharmacological and electrical studies has pinned down the main features of the mechanisms underlying the nerve impulse in the rat optic nerve,and this work proposed a mathematical model to simulate these phenomena.Methods The main active nodal channels:fast Na^+,persistent Na^,slow K^+ and a fast repolarizing K^+(A-current)were added on a double layer representation of the axon.A simplified representation of K^+ accumulation and clearance in the vicinity of the Ranvier node was integrated in this model.Results The model was able to generate the following features.In the presence of 4-aminopyridine (4-AP),spike duration increased and a depolarizing afterpotential(DAP)appeared.In the presence of 4-AP and tetraethylammonium(TEA),the DAP was followed by a hyperpolarizing afterpotential(AHP)and the amplitude of this AHP increased with the frequency of the stimulation.In normal conditions(no drugs):DAP and AHP were absent after a single action potential(AP)and a short train of AP;there was a relative refractoriness in amplitude lasting for 30 ms after an AP;an early AHP was revealed by a continuous depolarizing current;and there was a partial spike adaptation for a long current step stimulus.Conclusion The model successfully reproduced previous experiments results including long-lasting stimulation experiment,which is known to modify nerve physiological parameter values and is a key issue for visual prosthesis research. 展开更多
关键词 RAT optic nerve MODEL CHANNELS potassium homeostasis
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Heat shock induction of a 65 kDa ATP-binding proteinase in rat C6 glioma cells 被引量:8
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作者 XU CUN SHUAN WEI MING ZHANG +3 位作者 DIETER TECHEL MARCO MEYER YAN ZHANG LI LUDGER RENSING (Department of Biology, Henan Normal University,Xinxiang 453002)(Institute of Cell Biology, Bremen University, D-28359 Bremen, Germany) 《Cell Research》 SCIE CAS CSCD 1999年第2期135-144,共10页
The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their mol... The 45, 55, 65 and 100 kDa ATP-binding proteinases (ATP-BPases) of the heat-shocked (44 ℃ for 30 min, recovery for 12h) rat C6 glioma cells were purified by DEAE-ionexchange and ATP-affinity chromatography. Their molecular masses, isoelectric points (pI), pH-optima and other properties were analyzed by native proteinase gels.It was shown that the 65 kDa ATP-BPase is specifically induced by heat shock and not detectable in control cells.Its N-terminal 1-9 amino acid sequence was determined by Edman degradation, but no homologies to other proteins in the protein data bases were found. 30 and 31 kDa proteinases can be cleaved from the 45, 55 and 65 kDa proteinases to which they are linked. A possible relationship of the heat-induced 65 kDa ATP-BPase with the ATP-dependent proteinases (ATP-DPases) in prokaryotes and eukaryotes is discussed. 展开更多
关键词 Rat C6 glioma cells ATP-binding Proteinases heat shock induction native Proteinase gels
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Effects and mechanisms of store-operated calcium channel blockade on hepatic ischemia-reperfusion injury in rats 被引量:3
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作者 Li-Jie Pan,Zi-Chao Zhang,Zhen-Ya Zhang,Zong-Ming Zhang,Department of General Surgery,Digestive Medical Center,The First Affiliated Hospital,School of Medicine,Tsinghua University,Beijing 100016,China Wen-Jun Wang,Yue Xu,Xuyue (Beijing) Science and Technology Co.,Ltd.,Haidian District,Beijing 100080,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第4期356-367,共12页
AIM:To further investigate the important role of store-operated calcium channels (SOCs) in rat hepatocytes and to explore the effects of SOC blockers on hepatic ischemia-reperfusion injury (HIRI).METHODS:Using freshly... AIM:To further investigate the important role of store-operated calcium channels (SOCs) in rat hepatocytes and to explore the effects of SOC blockers on hepatic ischemia-reperfusion injury (HIRI).METHODS:Using freshly isolated hepatocytes from a rat model of HIRI (and controls),we measured cyto-solic free Ca 2+ concentration (by calcium imaging),net Ca 2+ fluxes (by a non-invasive micro-test technique),the SOC current (I SOC ;by whole-cell patch-clamp record-ing),and taurocholate secretion [by high-performance liquid chromatography and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays].RESULTS:Ca 2+ oscillations and net Ca 2+ fluxes medi-ated by Ca 2+ entry via SOCs were observed in rat he-patocytes.I SOC was significantly higher in HIRI groups than in controls (57.0 ± 7.5 pA vs 31.6 ± 2.7 pA,P <0.05) and was inhibited by La 3+.Taurocholate secretion by hepatocytes into culture supernatant was distinctly lower in HIRI hepatocytes than in controls,an effect reversed by SOC blockers.CONCLUSION:SOCs are pivotal in HIRI.SOC blockers protected against HIRI and assisted the recovery of se-cretory function in hepatocytes.Thus,they are likely to become a novel class of effective drugs for prevention or therapy of HIRI patients in the future. 展开更多
关键词 HEPATOCYTE Hepatic ischemia-reperfusion injury Store-operated calcium channel Non-invasive micro-test technique
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The Role of β3-adrenergic Receptor Gene in Neuropeptide Y Y5Receptor Antisense Gene Therapy of Diet-induced Obese Rats
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作者 张敏 李晓南 +5 位作者 郭锡熔 龚海霞 丁胜利 费莉 刘倩琦 陈荣华 《Journal of Nanjing Medical University》 2004年第1期16-20,共5页
Objective: To study the role of β3-adrenergic receptor gene in neuropeptide Y(NPY) Y5 receptor antisense gene therapy of diet-induced obese rats.Methods: The diet-induced obese rats were prepared by feeding a high-nu... Objective: To study the role of β3-adrenergic receptor gene in neuropeptide Y(NPY) Y5 receptor antisense gene therapy of diet-induced obese rats.Methods: The diet-induced obese rats were prepared by feeding a high-nutrition diet. Lateral ventricular was cannulated in obese rats which then received an intraventricular injection of either 5 μg/μl NPY Y5 receptor antisense or 10 μl missense oligodeoxynucleotide or saline of 10 μl respectively in every rat. When the rats were killed, the wet weight of abdominal adipose tissue, the level of serum lipid and lipoprotein were measured. Total RNA from the retroperitoneal adipose tissue was extracted and the level of β3-adrenergic receptor gene mRNA expression was evaluated by RT-PCR.Results: ①The wet weight of abdominal adipose tissue, the levels of serum lipids were greatly higher in diet-induced obese rats than those in normal rats. However, there were much lower β3-adrenergic receptor gene mRNA expression levels in retroperitoneal adipose tissue in diet-induced obese rats as compared with those in normal rats. ②After the diet-induced obese rats were intraventricularly administered with NPY Y5 receptor antisense oligodeoxynucleotide, the levels of β3-adrenergic receptor gene mRNA expression in retroperitoneal adipose tissue of diet-induced obese rats were strikingly up-regulated, whereas the wet weight of abdominal adipose tissue, the levels of serum lipids were markedly reduced.Conclusion: Intraventricular administration of antisense oligodeoxynucleotide to NPY Y5 receptor could significantly reduce the abdominal adipose tissue and the levels of serum lipids in diet-induced obese rats by up-regulating the level of β3-adrenergic receptor gene mRNA expression in retroperitoneal adipose tissue. 展开更多
关键词 adrenergic receptor OBESITY gene therapy receptor neuropeptide Y
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Isolation of neural progenitors from midbrain of newborn rats 被引量:1
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作者 李怡 娄淑杰 +1 位作者 何成 路长林 《Journal of Medical Colleges of PLA(China)》 CAS 2002年第3期157-161,共5页
Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunoc... Objective: To isolate neural progenitors from midbrain of new born rats. Methods: Cell groups with cloning capability were obtained from midbrain of new-born rats by using serum-free and floating cell culture. Immunocytochemistry was applied to detect antigens such as BrdU, Nestin and specific markers for mature neural cells. Results: Cell groups isolated from midbrain propagated in succession and shaped 'neuro-spheres'. Nestin antigen was expressed in the course of proliferation. After differentiation, epitopes of neuron , astrocyte and oligodendrocyte were all found in the neural progenitors-derived cells, the most majority were astrocyte-epitoped cells. Conclusion: Cell groups expressing Nestin isolated from midbrain of new-born rats have properties of propagating, multi-potency differentiating and self-renewal. It is demonstrated that the cell groups are stem-like neural progenitors in the central nervous system, which are qualified for cell therapy of diseases in central nervous system. 展开更多
关键词 neural progenitors ISOLATION cell culture
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Localization of vesicular glutamate transporters in the peripheral vesti-bular system of rat
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作者 王远 庞有旺 +3 位作者 董玉琳 张富兴 李金莲 李云庆 《Neuroscience Bulletin》 SCIE CAS CSCD 2007年第3期175-179,共5页
Objective To examine the vesicular glutamate transporters (VGluTs: VGluT 1-VGluT3) in the peripheral vestibular system. Methods The vestibular structures, including Scarpa's ganglion (vestibular ganglion, VG), m... Objective To examine the vesicular glutamate transporters (VGluTs: VGluT 1-VGluT3) in the peripheral vestibular system. Methods The vestibular structures, including Scarpa's ganglion (vestibular ganglion, VG), maculae of utricle and saccule, and ampullary cristae, from normal Sprague-Dawley rats were processed immunohistochemically for VGluTs, by avidin-biotinylated peroxidase complex method, with 3-3'-diaminobenzidine (DAB) as chromogen. Results (1) VGluT 1 was localized to partial neurons of VG and to the putative primary afferent fibers innervating vestibular end-organs. (2) Intense VGluT3 immunoreactivity was detected in large number of sensory epithelia cells, and weak labeling of VGluT3- positive afferent fibers was in the maculae and ampullary cristae. (3) No or very weak VGluT2 immunoreactivity was observed in the VG and acoustic maculae. Conclusion These results provide the morphological support that glutamate exists in the peripheral vestibular system, and it may play an important role in the centripetal vestibular transmission. 展开更多
关键词 vesicular glutamate transporter acoustic maculae Scarpa's ganglion IMMUNOHISTOCHEMISTRY RAT
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The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras
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作者 郭继彤 李雪峰 +6 位作者 Shahnaz Fida 苟克勉 Nakisa Malakooti ZHANG Chun-fang John R Morrison Alan O Trounson DU Zhong-tao 《Zoological Research》 CAS CSCD 北大核心 2009年第2期158-164,共7页
The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In ... The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In result, three rat chimeras were produced by day 5 (D5) Sprague-Dawley (SD) blastocysts injected with ICMs derived from day 6 (D6) and D5 Dark Agouti (DA) blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs arc able to contribute to chimeras, but their potential decreases significantly after culture in vitro (P〈0.05), and rFNS cells only have the potential to contribute to early fetal development. 展开更多
关键词 Rat chimeras Inner cell mass Rat fetal neural stem cells Blastocyst injection
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Neurogenesis by Activation of Inherent Neural Stem Cells in the Rat Hippocampus after Cerebral Infarction 被引量:14
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作者 Bo Zhang Ren-zhi wang +2 位作者 Zhi-gang Lian Yang Song Yong Yao 《Chinese Medical Sciences Journal》 CAS CSCD 2009年第1期41-45,共5页
Objective To investigate the changes of neural stem cells (NSCs) in the rat hippocampus after cerebral infarction (CI) and to evaluate the neurogenesis caused by the activation of NSCs. Methods CI models of rats were ... Objective To investigate the changes of neural stem cells (NSCs) in the rat hippocampus after cerebral infarction (CI) and to evaluate the neurogenesis caused by the activation of NSCs. Methods CI models of rats were made and rats were assigned to 6 groups: sham-operated, 1 day, 3 days, 7 days, 14 days, and 28 days after CI. The dynamic expression of bromodeoxyuridine (BrdU), polysialylated neural cell adhesion molecule (PSA-NCAM), glial fibrillary acidic protein (GFAP), and neuronal nuclear antigen (NeuN) were determined by immunohistochemistry and immunofluorescence staining. BrdU was used to mark the proliferated NSCs. PSA-NCAM was used to mark the plasticity of activated NSCs. GFAP and NeuN were used to mark the differentiated NSCs. Results Compared with the controls, the number of BrdU+ cells in the hippocampus increased significantly at 1 day after CI (P<0.05), reached peak at 7 days after CI (P<0.05), decreased but still elevated compared with the controls at 14 days after CI (P<0.05), and nearly unchanged at 28 days after CI. The number of BrdU+/PSA-NCAM+ cells increased significantly at 7 days after CI (P<0.05), reached peak at 14 days after CI (P<0.05), and decreased but still elevated compared with the controls at 28 days after CI (P<0.05). The number of BrdU+/PSA-NCAM+ cells was equal to 60% of the number of BrdU+ cells in all the same period. The number of BrdU+/NeuN+ cells in the hippocampus increased significantly at 14 days after CI (P<0.05) and reached peak at 28 day after CI (P<0.05). The number of BrdU+/GFAP+cells in the hippocampus nearly unchanged after CI. Conclusion CI can stimulate the proliferation of inherent NSCs, and most proliferated NSCs may differentiate into neurons and represent neural plasticity. 展开更多
关键词 cerebral infarction neural stem cells NEUROGENESIS HIPPOCAMPUS
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Effects of carbohydrate supplements on exercise-induced menstrual dysfunction and ovarian subcellular structural changes in rats
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作者 Can Zhao Xiao-Li Liu +3 位作者 Run-Xiao Hong He Li Rena Li Ren-Wei Wang 《Journal of Sport and Health Science》 SCIE 2014年第3期189-195,共7页
Background: Exercise-associated menstrual dysfunction (EAMD) is a common health problem in female athletes as a part of female athlete triad (FAT), a condition related to low energy availability. In this study, w... Background: Exercise-associated menstrual dysfunction (EAMD) is a common health problem in female athletes as a part of female athlete triad (FAT), a condition related to low energy availability. In this study, we explored the possibility that carbohydrate supplements can improve the status of EAMD and prevent exercise-induced ovarian injury in a FAT rat model. This research aimed to provide experimental evidence with regard to the relationship of energy intervention and EAMD. Methods: Forty-five female Sprague-Dawley rats (2 months old) were randomly divided into five experimental groups: control group (C), 9-week exercise as model for EAMD (E), post-EAMD recovery group (R), oligosaccharide intervention group (O), and glucose intervention group (G). All rats were sacrificed at the end of 9 weeks. Serum samples were collected for measuring gonadotropin releasing hormone, follicle stimulating hormone, luteinizing hormone, 1713-estradiol and progesterone levels. The ovaries were taken for investigation of exercise- and carbohydrate-induced follicular subcellular structure changes. Results: Exercise induced irregular menstrual cycles and ovary subcellular structural damages, such as swollenness of mitochondria in rats from groups E and R. Both glucose and oligosaccharide supplements restored well-differentiated mitochondria in the ovarian follicular cells, and a significant improvement of endoplasmic reticulum and Golgi in swollenness in theca cells in groups O and G compared to groups C, E, and R. There was no difference in mitochondria subcellular structural changes between groups O and G. Group E showed attenuation of serum levels of 17β-estradiol and progesterone compared to C. There were no differences of 17β-estradiol serum levels among groups O, G, and R, while group G showed a lower level of progesterone than C. Conclusion: Female adult rats with 9-week continuous exercise can cause menstrual dysregulation as a model for EAMD. Post-EAMD intervention with glucose and oligosaccharide intake can normalize the menstrual cycle, restore the follicular subcellular structure, and reverse the exercise-induced reduction of ovary sex hormones. It suggests a positive feedback of hypothalamus-pituitary-ovary axis might be involved in the molecular mechanisms of energy intake in treating EAMD. 展开更多
关键词 Carbohydrate supplement ESTRADIOL Estrous cycle restrain Follicular subcellular injury Ovary PROGESTERONE RATS
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Effect of neurotrophin-3 on SOD and MDA in rats after acute spinal cord injury 被引量:3
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作者 郭树章 任先军 +1 位作者 蒋涛 欧阳忠 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第1期28-30,共3页
Objective:To investigate the effect of neurotrophin-3 on the expressions of SOD and MDA in the injured spinal cord of rats. Methods: Totally 105 SD rats were randomly divided into 3 groups (n = 35): sham group, contro... Objective:To investigate the effect of neurotrophin-3 on the expressions of SOD and MDA in the injured spinal cord of rats. Methods: Totally 105 SD rats were randomly divided into 3 groups (n = 35): sham group, control group and experimental group. Animal model of acute spinal cord was inflicted with Allen's method by a thin plastic tube situated in subarachnoid space below the injury level for perfusion. Rats in experimental group received 20μl NT-3 (200 ng) from the tube at 0, 4. 8. 12. 24 h and 3. 7 d after injury, and those in control group got the equal volume of normal saline at the same time points. The animals in sham group only received opening vertebral plate and putting tube in subarachnoid space. The rats were sacrificed at 4, 8. 12. 24 h, and 3. 7, 14 d postinjury (n = 5). And the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in blood were observed with colorimetric method. Results: The serum level of SOD reduced obviously and the level of MDA raised obviously in rats after the injury, and the activity of SOD reached the lowest on day 3 and the concentration of MDA reached peak at the 7 d. In the experimental group, the SOD level was obviously higher (P<0. 01). and MDA level was lower than the control (P<0. 01). Conclusion:NT-3 can mitigate secondary injury of spinal cord in vivo. One of mechanisms is that inhibits abnormal expression of MDA and elevates the activity of SOD. thus the injury of free radical and lipid peroxidation is attenuated. 展开更多
关键词 NEUROTROPHIN-3 spinal cord injury superoxide dismutase MALONDIALDEHYDE
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