Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinat...Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinating diseases. However, some fundamental cellular properties of OECs remain unclear. In this study, we found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations. Moreover, OEC subpopulations displayed different attractive migratory responses to a gradient of lysophosphatidic acid (LPA) in single-cell migration assays. Finally, we found that OEC subpopulations transformed into each other spontaneously. Together, these results demonstrate, for the first time to our knowledge, that distinct OEC subpopulations display different migratory properties in vitro and provide new evidence to support the notion of OECs as a single cell type with malleable functional phenotypes.展开更多
AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to disso...AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc., were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC.展开更多
By using image recognition technology, the underground bin level can be detdcted. The bin image is noised by vibration, atomy, backgroun and so on. The image restoration and image mathematical morphology were used bas...By using image recognition technology, the underground bin level can be detdcted. The bin image is noised by vibration, atomy, backgroun and so on. The image restoration and image mathematical morphology were used based on neural network. A modified Hopfield network was presented for image restoration. The greed algorithm with n-simultaneous updates and apartially asynchronous algorithm were combined, im- proving convergence and avoiding synchronization penalties. Mathematical morphology was widely applicated in digital image processing. The basic idea of mathematical mor- phology is to use construction element measure image morphology for solving under- stand problem. Presented advanced Cellular neural network that forms MMCNN equa- tion to be suit for mathematical morphology filter. It gave the theory of MMCNN dynamic extent and stable state. It was evidenced that arrived mathematical morphology filter through steady of dynamic precess in definite condition. The results of implementation were applied in detecting undergroug bin level.展开更多
Economic movements have close relation with historical transformations. Historical and social transformations have seriously determined economic views, so, economic movements have been the indicators of economic wars ...Economic movements have close relation with historical transformations. Historical and social transformations have seriously determined economic views, so, economic movements have been the indicators of economic wars of social classes. But no concept can describe sharp contrast in economics better than the two opponent concepts in economics: "orthodox" and "heterodox" economics. In this article, the reason why neo-classic economics, also called as orthodox economics, has a serious place in economic literature while the opponent's economic movements, named heterodox economics, do not defend only one "truth" and are not as important as orthodox economics will be examined. While doing this examination, Louis Althusser's "ideology" and Antonio Gramsci's "hegemony" will help us as these two concepts are quite instructive in understanding the irreplaceable significance of orthodox economics. As a result, by discussing alternative point of views about economics, positive emphasis of multivocality in economics literature will be revealed. On the other hand, every heterodox economics cannot criticize orthodox economics in the same way. In this study, institutional economics, which is accepted to be part of heterodox economics, will be discussed thoroughly. Institutional economics had a serious attitude against orthodox economics. In this study, generally the points in orthodox economics that institutional economics opposes will be emphasized, and although both economics approaches' ideological attitude will be attempted to be discussed generally, it will become easy to discuss the reason why heterodox economics developed an opposing ideology against the ideology of orthodox economics.展开更多
The paper analyzes the current trends of urban transformation and delineates an underlying rationale which enables the comprehension of the current character of Arab city with particular reference to Al-Khobar. The ci...The paper analyzes the current trends of urban transformation and delineates an underlying rationale which enables the comprehension of the current character of Arab city with particular reference to Al-Khobar. The city form is subjected to a re-reading arguments of which are based on critical interpretation of the mechaitisms of urban morphology in relation not only to the impacts of global economy and building boom but also to the means of perception of urban space by different strata of the urban realm. Thus, urban morphology of the city is studied as a medium which manifests a dissociation, and thus duality, between social structure and its physical container. The paper further argues that uncritical import of urban and architectural typologies seem to create a peculiar type of urban-architectural forgery that is usually reduced to skin-deep facade mimicry. Particularly, solo performances of shiny high-rise buildings and sharp contrasts of street fronts between main arteries and back streets depict impressions of fragmentation and segregation. The emerging urban-scape that can be associated with the cultural and economic leap in Arab regions today is critically evaluated. Thus, the paper proposes judicious and critical planning policy instead of direct import of global urban-architectural typologies.展开更多
With the development of technology,the learning and memory functions of artificial memristor synapses are necessary for realizing artificial neural networks and neural neuromorphic computing.Owing to their high scalab...With the development of technology,the learning and memory functions of artificial memristor synapses are necessary for realizing artificial neural networks and neural neuromorphic computing.Owing to their high scalability performance,nanosheet materials have been widely employed in cellular-level learning,but the behaviors of nociceptor based on nanosheet materials have rarely been studied.Here,we present a memristor with an Al/TiO_(2)/Pt structure.After electroforming,the memristor device showed a gradual conductance regulation and could simulate synaptic functions such as the potentiation and depression of synaptic weights.We also designed a new scheme that verifies the pain sensitization,desensitization,allodynia,and hyperalgesia behaviors of real nociceptors in the fabricated memristor.Memristors with these behaviors can significantly improve the quality of intelligent electronic devices.Data fitting showed that the high resistance and low resistance states were consistent with the hopping conduction mechanism.This work promises the application of TiO_(2)-based devices in next-generation neuromorphological systems.展开更多
Aggression is a common behavioral trait shared in many animals, including both vertebrates and invertebrates. However, the type and intensity of agonistic encounters and displays can vary widely both across and within...Aggression is a common behavioral trait shared in many animals, including both vertebrates and invertebrates. However, the type and intensity of agonistic encounters and displays can vary widely both across and within species, resulting in complicated or subjective interpretations that create difficulties in developing theoretical models that can be widely applied. The need to easily and objectively identify quantifiable behaviors and their associated morphologies becomes especially important when attempting to decipher the neurological mechanisms underlying this complex behavior. Monoamines, neuropeptides, and pheromones have been implicated as important neuromodulators for agonistic displays in both invertebrates and vertebrates. Ad- ditionally, recent breakthroughs in insect research have revealed exciting proximate mechanisms important in aggression that may be broadly relevant, due to the relatively high conservation of these neurochemical systems across animal taxa. In this review, we present the latest research demonstrating the importance of monoamines, neuropeptides, and pheromones as neuromodulators for aggression across a variety of insect species. Additionally, we describe the stalk-eyed fly as a model system for studying aggres- sion, which integrates physiological, morphological, and neurochemical approaches in exploring detailed mechanisms responsible for this common yet complex behavior. We conclude with our perspective on the most promising lines of future research aimed at understanding the proximate and ultimate mechanisms underlying aggressive behaviors .展开更多
This paper studies the state/output synchronization of switched Boolean networks (SBNs) with impulsive effects via the algebraic state space representation (ASSR) approach. First, an algebraic form is established ...This paper studies the state/output synchronization of switched Boolean networks (SBNs) with impulsive effects via the algebraic state space representation (ASSR) approach. First, an algebraic form is established for SBNs with impulsive effects via ASSR. Second, based on the algebraic form, some necessary and sufficient conditions are presented for the state/output synchronization of SBNs with impulsive effects under arbitrary switching signals. Third, two special kinds of switching signals, that is, free switching signal and feedback switching signal, are considered for the state synchroniza-tion of SBNs with impulsive effects. Finally, two illustrative examples are worked out to show the effectiveness of the obtained results.展开更多
Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whe...Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whether the cellular morphology of neurons in these regions has evolved in association with the relative use of a modality.We investigated relationships between the behavioral use of communication modalities and neural morphologies in six lizard species. Two of these species (Anolis carol# nensis and Leiocephalus carinatus) primarily use visual signals to communicate with conspecifics and detect potential prey, and two (Aspidoscelis gularis and Scincella lateralis) communicate and forage primarily using chemical signals. Two other species (Hemidactylus turcicus and Sceloporus olivaceus) use both visual and chemical signals. For each species, we performed beha- vioral observations and quantified rates of visual and chemical behaviors. We then cryosectioned brain tissues from 9-10 males of each species and measured the soma size and density of neurons in two brain regions associated with visual behaviors (the lat- eral geniculate nucleus and the nucleus rotundus) and one region associated with chemical behaviors (the nucleus sphericus). With analyses conducted in a phylogenetic context, we found that species that performed higher rates of visual displays had a denser lateral geniculate nucleus, and species that used a higher proportion of chemical displays had larger somas in the nucleus sphericus. These relationships suggest that neural morphologies in the brain have evolved convergently in species with similar communication behaviors .展开更多
Solid electrolyte based-resistive memories have been considered to be a potential candidate for future information technology with applications in non-volatile memory, logic circuits and neuromorphic computing. A cond...Solid electrolyte based-resistive memories have been considered to be a potential candidate for future information technology with applications in non-volatile memory, logic circuits and neuromorphic computing. A conductive filament model has been generally accepted to be the underlying mechanism for the resistive switching. However, the growth dynamics of such conductive filaments is still not fully understood. Here, we explore the controllability of filament growth by correlating observations of the filament growth with the electric field distribution and several other factors. The filament growth behavior has been recorded using in situ transmission electron microscopy. By studying the real- time recorded filament growth behavior and morphologies, we have been able to simulate the electric field distribution in accordance with our observations. Other factors have also been shown to affect the filament growth, such as Joule heating and electrolyte infrastructure. This work provides insight into the controllable growth of conductive filaments and will help guide research into further functionalities of nanoionic resistive memories.展开更多
基金We thank Drs Chenbing Guan and Kui Cui (Institute of Neuroscience, Shanghai Institute for Biological Science, China) for technical support in setting up the single-cell migration assay, and Dr Qian Hu (Institute of Neuroscience, Shanghai Institute for Biological Science, China) for microscopic imaging. This study was supported by the National Key Basic Research Program (2006CB500702), Ministry of Science and Technology of China (2007CB947100), National Natural Science Foundation of China (30530240 and 30770657), Program for Changdiang Scholars and Innovative Research Teams in Universities (IRT0528), and Shanghai Metropolitan Fund for Research and Development (07DJ14005).
文摘Olfactory ensheathing cells (OECs) are a unique type of glial cells that have axonal growth-promoting properties. OEC transplantation has emerged as a promising experimental therapy of axonal injuries and demyelinating diseases. However, some fundamental cellular properties of OECs remain unclear. In this study, we found that the distinct OEC subpopulations exhibited different migratory properties based on time-lapse imaging of single isolated cells, possibly due to their different cytoskeletal organizations. Moreover, OEC subpopulations displayed different attractive migratory responses to a gradient of lysophosphatidic acid (LPA) in single-cell migration assays. Finally, we found that OEC subpopulations transformed into each other spontaneously. Together, these results demonstrate, for the first time to our knowledge, that distinct OEC subpopulations display different migratory properties in vitro and provide new evidence to support the notion of OECs as a single cell type with malleable functional phenotypes.
基金Supported by the National Natural Science Foundation of China, No. 30300156
文摘AIM: To study the method of dissociation, culture and investigate its morphologic changes in vitro of interstitial cells of Cajal (ICC).METHODS: Enzymatic digestion and Ficoll density centrifugation were used to dissociate ICC from the ileal segment of mice. Factors including contamination, Ca2+, Mg2+ and collagenase, and stem cell factor, etc., were investigated.ACK2, the antibody of c-kit, was used to identify the cultured ICC. Both light microscope and fluorescence microscope were used to observe the changes of ICC in vitro.RESULTS: The method for dissociation and culture of ICC in vitro was successfully established. After 24 h, cultured ICC exhibited a few axis-cylinders, and longer axis-cylinders were observed to form synapse of each other after 3 d. More widespread connections formed within 7 d in vitro. The changes of its morphologic character were obvious within 7 d; however, there were no obvious morphologic changes after 30 d.CONCLUSION: Many factors can influence the dissociation and culture of ICC.
文摘By using image recognition technology, the underground bin level can be detdcted. The bin image is noised by vibration, atomy, backgroun and so on. The image restoration and image mathematical morphology were used based on neural network. A modified Hopfield network was presented for image restoration. The greed algorithm with n-simultaneous updates and apartially asynchronous algorithm were combined, im- proving convergence and avoiding synchronization penalties. Mathematical morphology was widely applicated in digital image processing. The basic idea of mathematical mor- phology is to use construction element measure image morphology for solving under- stand problem. Presented advanced Cellular neural network that forms MMCNN equa- tion to be suit for mathematical morphology filter. It gave the theory of MMCNN dynamic extent and stable state. It was evidenced that arrived mathematical morphology filter through steady of dynamic precess in definite condition. The results of implementation were applied in detecting undergroug bin level.
文摘Economic movements have close relation with historical transformations. Historical and social transformations have seriously determined economic views, so, economic movements have been the indicators of economic wars of social classes. But no concept can describe sharp contrast in economics better than the two opponent concepts in economics: "orthodox" and "heterodox" economics. In this article, the reason why neo-classic economics, also called as orthodox economics, has a serious place in economic literature while the opponent's economic movements, named heterodox economics, do not defend only one "truth" and are not as important as orthodox economics will be examined. While doing this examination, Louis Althusser's "ideology" and Antonio Gramsci's "hegemony" will help us as these two concepts are quite instructive in understanding the irreplaceable significance of orthodox economics. As a result, by discussing alternative point of views about economics, positive emphasis of multivocality in economics literature will be revealed. On the other hand, every heterodox economics cannot criticize orthodox economics in the same way. In this study, institutional economics, which is accepted to be part of heterodox economics, will be discussed thoroughly. Institutional economics had a serious attitude against orthodox economics. In this study, generally the points in orthodox economics that institutional economics opposes will be emphasized, and although both economics approaches' ideological attitude will be attempted to be discussed generally, it will become easy to discuss the reason why heterodox economics developed an opposing ideology against the ideology of orthodox economics.
文摘The paper analyzes the current trends of urban transformation and delineates an underlying rationale which enables the comprehension of the current character of Arab city with particular reference to Al-Khobar. The city form is subjected to a re-reading arguments of which are based on critical interpretation of the mechaitisms of urban morphology in relation not only to the impacts of global economy and building boom but also to the means of perception of urban space by different strata of the urban realm. Thus, urban morphology of the city is studied as a medium which manifests a dissociation, and thus duality, between social structure and its physical container. The paper further argues that uncritical import of urban and architectural typologies seem to create a peculiar type of urban-architectural forgery that is usually reduced to skin-deep facade mimicry. Particularly, solo performances of shiny high-rise buildings and sharp contrasts of street fronts between main arteries and back streets depict impressions of fragmentation and segregation. The emerging urban-scape that can be associated with the cultural and economic leap in Arab regions today is critically evaluated. Thus, the paper proposes judicious and critical planning policy instead of direct import of global urban-architectural typologies.
基金financially supported by the National Natural Science Foundation of China(61674050 and 61874158)the Project of Distinguished Youth of Hebei Province(A2018201231)+5 种基金the Hundred Persons Plan of Hebei Province(E2018050004 and E2018050003)the Supporting Plan for 100 Excellent Innovative Talents in Colleges and Universities of Hebei Province(SLRC2019018)the Special Project of Strategic Leading Science and Technology of Chinese Academy of Sciences(XDB44000000-7)the Outstanding Young Scientific Research and Innovation Team of Hebei Universitythe Highlevel Talent Research Startup Project of Hebei University(521000981426)the Special Support Funds for National High Level Talents(041500120001 and 521000981429)。
文摘With the development of technology,the learning and memory functions of artificial memristor synapses are necessary for realizing artificial neural networks and neural neuromorphic computing.Owing to their high scalability performance,nanosheet materials have been widely employed in cellular-level learning,but the behaviors of nociceptor based on nanosheet materials have rarely been studied.Here,we present a memristor with an Al/TiO_(2)/Pt structure.After electroforming,the memristor device showed a gradual conductance regulation and could simulate synaptic functions such as the potentiation and depression of synaptic weights.We also designed a new scheme that verifies the pain sensitization,desensitization,allodynia,and hyperalgesia behaviors of real nociceptors in the fabricated memristor.Memristors with these behaviors can significantly improve the quality of intelligent electronic devices.Data fitting showed that the high resistance and low resistance states were consistent with the hopping conduction mechanism.This work promises the application of TiO_(2)-based devices in next-generation neuromorphological systems.
文摘Aggression is a common behavioral trait shared in many animals, including both vertebrates and invertebrates. However, the type and intensity of agonistic encounters and displays can vary widely both across and within species, resulting in complicated or subjective interpretations that create difficulties in developing theoretical models that can be widely applied. The need to easily and objectively identify quantifiable behaviors and their associated morphologies becomes especially important when attempting to decipher the neurological mechanisms underlying this complex behavior. Monoamines, neuropeptides, and pheromones have been implicated as important neuromodulators for agonistic displays in both invertebrates and vertebrates. Ad- ditionally, recent breakthroughs in insect research have revealed exciting proximate mechanisms important in aggression that may be broadly relevant, due to the relatively high conservation of these neurochemical systems across animal taxa. In this review, we present the latest research demonstrating the importance of monoamines, neuropeptides, and pheromones as neuromodulators for aggression across a variety of insect species. Additionally, we describe the stalk-eyed fly as a model system for studying aggres- sion, which integrates physiological, morphological, and neurochemical approaches in exploring detailed mechanisms responsible for this common yet complex behavior. We conclude with our perspective on the most promising lines of future research aimed at understanding the proximate and ultimate mechanisms underlying aggressive behaviors .
基金The research was supported by the National Natural Science Foundation of China under grant 61503225, the Natural Science Fund for Distinguished Young Scholars of Shandong Province under grant JQ201613, and the Natural Science Foundation of Shandong Province under grant ZR2015FQ003.
文摘This paper studies the state/output synchronization of switched Boolean networks (SBNs) with impulsive effects via the algebraic state space representation (ASSR) approach. First, an algebraic form is established for SBNs with impulsive effects via ASSR. Second, based on the algebraic form, some necessary and sufficient conditions are presented for the state/output synchronization of SBNs with impulsive effects under arbitrary switching signals. Third, two special kinds of switching signals, that is, free switching signal and feedback switching signal, are considered for the state synchroniza-tion of SBNs with impulsive effects. Finally, two illustrative examples are worked out to show the effectiveness of the obtained results.
基金We thank the members of the Johnson Lab at Trinity University for assistance in the field and labor- atory - particularly Bonnie Kircher, Alisa Dill, Michelle Ob- emdorf, Adam Silva, Taren Blackmon, Jordan Bush, McKen- zie Quinn, EUee Cook, Grayam Sailor-Tynes, and Collin Shinkle. We also thank David Ribble, Troy Murphy, Jimmy Roberts, Kevin McIntyre, and three reviewers for comments on an earlier version of this work, and Kevin McIntyre and Kimberly Phillips for statistical advice. This work was funded by a Sigma Xi GIAR (to C.D.R.), a Trinity University Mind Science Foundation grant (to C.D.R.), Trinity University's HHMI and Biology Summer Undergraduate Research Fel- lowships (to C.D.R.), Texas Ecolab, and the National Science Foundation (IOS 1257021 to M.A.J.). All procedures were approved by Trinity University's Animal Research Committee (protocol # 050213_MAJ2).
文摘Animals communicate information within their environments via visual, chemical, auditory, and/or tactile modalities. The use of each modalityis generally linked to particular brain regions, but it is not yet known whether the cellular morphology of neurons in these regions has evolved in association with the relative use of a modality.We investigated relationships between the behavioral use of communication modalities and neural morphologies in six lizard species. Two of these species (Anolis carol# nensis and Leiocephalus carinatus) primarily use visual signals to communicate with conspecifics and detect potential prey, and two (Aspidoscelis gularis and Scincella lateralis) communicate and forage primarily using chemical signals. Two other species (Hemidactylus turcicus and Sceloporus olivaceus) use both visual and chemical signals. For each species, we performed beha- vioral observations and quantified rates of visual and chemical behaviors. We then cryosectioned brain tissues from 9-10 males of each species and measured the soma size and density of neurons in two brain regions associated with visual behaviors (the lat- eral geniculate nucleus and the nucleus rotundus) and one region associated with chemical behaviors (the nucleus sphericus). With analyses conducted in a phylogenetic context, we found that species that performed higher rates of visual displays had a denser lateral geniculate nucleus, and species that used a higher proportion of chemical displays had larger somas in the nucleus sphericus. These relationships suggest that neural morphologies in the brain have evolved convergently in species with similar communication behaviors .
文摘Solid electrolyte based-resistive memories have been considered to be a potential candidate for future information technology with applications in non-volatile memory, logic circuits and neuromorphic computing. A conductive filament model has been generally accepted to be the underlying mechanism for the resistive switching. However, the growth dynamics of such conductive filaments is still not fully understood. Here, we explore the controllability of filament growth by correlating observations of the filament growth with the electric field distribution and several other factors. The filament growth behavior has been recorded using in situ transmission electron microscopy. By studying the real- time recorded filament growth behavior and morphologies, we have been able to simulate the electric field distribution in accordance with our observations. Other factors have also been shown to affect the filament growth, such as Joule heating and electrolyte infrastructure. This work provides insight into the controllable growth of conductive filaments and will help guide research into further functionalities of nanoionic resistive memories.