经皮神经电针刺激对骨质疏松大鼠骨形态发生蛋白-2表达的影响

目的探讨经皮神经电针刺激对骨质疏松大鼠骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)表达的影响。方法选择88只4个月大雌性SD大鼠,采用随机数字法将其分为空白组(不做任何处理,n=22)、假手术组(只暴露双侧卵巢但不切除,n=22)、模型组(双侧卵巢切除术,n=22)、电针刺激组(双侧卵巢切除术+经皮神经电针刺激,n=22)。干预结束后,测定4组大鼠离体骨组织骨密度;分别采用第4腰椎压缩试验、股骨三点弯曲试验测定大鼠腰椎、股骨生物力学;采用Real-time PCR检测大鼠血清BMP-2、转化生长因子β1(transforming growth factor-β1,TGF-β1)表达水平,采用改良Mankin评分综合评定大鼠骨组织形态及结构。结果模型组大鼠股骨、腰椎骨密度低于空白组及假手术组,电针刺激组高于模型组、低于空白组及假手术组(P<0.05)。第4腰椎压缩试验及股骨三点弯曲试验结果:模型组大鼠弹性模量、最大载荷低于空白组及假手术组,电针刺激组高于模型组、低于空白组及假手术组(P<0.05)。模型组大鼠Mankin评分高于空白组及假手术组,电针刺激组低于模型组、高于空白组及假手术组(P<0.05)。模型组大鼠血清BMP-2、TGF-β1 mRNA低于空白组及假手术组,电针刺激组高于模型组、空白组及假手术组(P<0.05)。结论经皮神经电针刺激可提高骨质疏松大鼠血清BMP-2表达水平,增强骨密度,可有效抑制骨质疏松与关节软骨退化。

Treatment of Early-stage Adhesive Shoulder Periarthritis with Transcutaneous Electric Stimulation on Acupoints

Objective： To observe the therapeutic effects of transcutaneous electric acupoint stimulation on early-stage adhesive shoulder periarthritis. Methods： by using the research approach of evidence-based medicine such as multi-center, large sample and randomization, the 163 cases of early-stage adhesive shoulder periarthritis were treated with transcutaneous electric point stimulation and electric acupuncture respectively to observe the therapeutic effect of transcutaneous electric acupoint stimulation and compare its effect with electric acupuncture. Results and Conclusion： The total effective rate of transcutaneous electric point stimulation on early-stage adhesive shoulder periarthritis reached 96.5%, showing no significant difference with the electric acupuncture group; the transcutaneous electric acupoint stimulation could not only relieve pain, but also improve the shoulder joint movement. As a result, the transcutaneous electric acupoint stimulation is an easy and more effective therapy for shoulder periarthritis.