rDNA的修饰作用改善了蛋白C的活化作用(此法对其他rDNA蛋白可能有用)

象许多经商业开发的蛋白一样,蛋白C生产出来后是无活性、单链的、其必须经裂解后才能被激活。重复地进行激活是无效的。然而,上周在西德杜塞尔多夫举行的 BIOTEC88年会上,Hartmut Ehrlich 向与会者解释了他发明的用于提高 rDNA 蛋白 C(一种潜在的抗凝剂和潜在的血栓溶解素)活性的方法。

我渴了

主耶稣在十字架上,遍体鳞伤,体无完肤。他的两手与双脚被粗笨的铁钉钉住。钉痕的伤口已经裂开,殷红的鲜血不断地往外面倾流。鲜血染红了十字架。 灰暗的天空,茫茫的大地,十字架屹立在各各他山岗。

农历本伴我人生路

多年来,我的衣兜里总是藏着一本小小的农历本,无论是几年前我在三尺讲台播种的过去,还是坐机关“爬格子”的现在,它总像一位忠实的朋友时刻陪伴着我,倾听我的酸甜苦辣,给我以前进的力量。我的农历本就是大街小巷摆地摊卖的二元钱左右的小本本。

古代名人谈养生

人到老年,若能乐观旷达,事事无忧无虑,做到心神怡悦就可以永年。 我国有五千年的文明史,古人对于养生之道颇有研究,历代名人亦有诗文谈及此道。 孔子说:“在邦无怨,在家无怨,”又说:“不怨天。

Reservoir reconstruction technologies for coalbed methane recovery in deep and multiple seams

Multiple coal seams widely develop in the deep Chinese coal-bearing strata. Ground in situ stress and coal seam gas pressure increase continuously with the increase of the mining depth, and coal and gas outburst disasters become increasingly severe. When the coal is very deep, the gas content and pressure will elevate and thus coal seams tends to outburst-prone seams. The safety and economics of exploited firstmined coal seams are tremendously restricted. Meanwhile, the multiple seams occurrence conditions resulted in different methane pressure systems in the coal-bearing strata, which made the reservoir reconstruction of coal difficult. Given the characteristics of low saturation, low permeability, strong anisotropy and soft coal of Chinese coal seams, a single hydraulic fracturing surface well for reservoir reconstruction to pre-drain the coalbed methane(CBM) of multiple seams concurrently under the different gas pressure systems has not yet gained any breakthroughs. Based on analyses of the main features of deep CBM reservoirs in China, current gas control methods and the existing challenges in deep and multiple seams, we proposed a new technology for deep CBM reservoir reconstruction to realize simultaneous high-efficiency coal mining and gas extraction. In particular, we determined the first-mined seam according to the principles of effectiveness and economics, and used hydraulic fracturing surface well to reconstruct the first-mined seam which enlarges the selection range of the first-mined seam. During the process of mining first-mined seam, adjacent coal seams could be reconstructed under the mining effect which promoted high-efficiency pressure relief gas extraction by using spatial and comprehensive gas drainage methods(combination of underground and ground CBM extraction methods). A typical integrated reservoir reconstruction technology, ‘‘One well for triple use", was detailed introduced and successfully applied in the Luling coal mine. The application showed that the proposed technology could effectively promote coal mining safety and simultaneously high-efficiency gas extraction.

Intelligent identifi cation method for near-surface ground fi ssures based on seismic data

Taking a study area in Jinzhong Basin in Qixian County,Shanxi Province,as an example,this work performs an intelligent interpretation of ground fissures.On the basis of a complete analysis of the regional geological background in the study area,dip-steering cube operation and median filtering of seismic data were performed using fast Fourier transform to improve the continuity of seismic events and eliminate random noise.A total of 200 stratigraphic continuous sample training points and 500 discontinuous training points were obtained from the processed seismic data.Thereafter,a variety of attributes(coherence,curvature,amplitude,frequency,etc.)were extracted as the input for the multilayer perceptron neural network training.During the training period,the training results were traced by normalized root mean square error(RMSE)and misclassifi cation.The training results showed a downward trend during the training period.The misclassifi cation curve was stable at 0.3,and the normalized RMSE curve was stable at 0.68.When the value of the normalized RMSE curve reached the minimum,the training was terminated,and the training results were extended to the whole data volume to obtain the attribute cube of intelligent ground fi ssure detection.The characteristics of ground fi ssures were analyzed and identifi ed from the sections and slices.A total of 11 ground fissures were finally interpreted.The interpretation results showed that the dip angles were 60°-85°,the fault throws were 0-43 m,and the extension lengths were 300-1,100 m in the whole area.The strike of 73%of the ground fi ssures was consistent with the direction of the regional tectonic settings.Specifi cally,four ground fi ssures coincided with the surface disclosed,and the verifi cation rate reached 100%.In conclusion,the intelligent ground fi ssure detection attribute based on the dip-steering cube is eff ective in predicting the spatial distribution of ground fi ssures.

Neurotoxicity and Biomarkers of Lead Exposure: a Review

Appropriate selection and measurement of lead biomarkers of exposure are critically important for health care management purposes,public health decision making,and primary prevention synthesis.Lead is one of the neurotoxicants that seems to be involved in the etiology of psychologies.Biomarkers are generally classified into three groups:biomarkers of exposure,effect,and susceptibility.The main body compartments that store lead are the blood,soft tissues,and bone;the half-life of lead in these tissues is measured in weeks for blood,months for soft tissues,and years for bone.Within the brain,lead-induced damage in the prefrontal cerebral cortex,hippocampus,and cerebellum can lead to a variety of neurological disorders,such as brain damage,mental retardation,behavioral problems,nerve damage,and possibly Alzheimer’s disease,Parkinson’s disease,and schizophrenia.This paper presents an overview of biomarkers of lead exposure and discusses the neurotoxic effects of lead with regard to children and adults.

Paul Tillich＇s Schizophrenia

Paul Tillich once confided to his secretary at Harvard that he was a schizophrenic. In this article, the author, who is also a schizophrenic, discusses symptoms of schizophrenia in Tillich＇s biography and theology. Tillich had always insisted that his theology was one of the kairos. By hiding his theological thought behind an arcane Greek word, Tillich dissimulated his own messiah complex. On several occasions, Tillich describes a mystical schizophrenic hallucination with such accuracy that it is obvious to those who have had similar visions that he was speaking from personal experience. This article raises unanswerable questions about the relationships between religious revelation, mystical ecstasy and schizophrenia.

Effect of Operating Parameters on Composition of Dry Gas Obtained During Gasoline Cracking Process

The influence of operating parameters on ethylene content in dry gas obtained during catalytic cracking of gasoline was investigated in a pilot fixed fluidized bed reactor in the presence of the MMC-2 catalyst. The results have shown that the majority of dry gas was formed during the catalytic cracking reaction of gasoline, with a small proportion of dry gas being formed through the thermal cracking reaction of gasoline. The ethylene content in dry gas formed during the catalytic cracking reaction was higher than that in dry gas formed during the thermal cracking reaction. The ethylene content in dry gas formed during catalytic cracking of gasoline with a higher olefin content was higher than that in dry gas formed during catalytic cracking of gasoline with a lower olefin content, which meant that the higher the amount of carbonium ions was produced during the reaction, the higher the ethylene content in the dry gas would be. An increasing reaction temperature could increase the percentage of dry gas formed during thermal cracking reaction in total dry gas products, leading to decreased ethylene content in the dry gas. An increasing catalyst/oil ratio could be conducive to the catalytic cracking reactions taking place inside the zeolite Y, leading to a decreased ethylene content in the dry gas. A decreasing space velocity could be conducive to the catalytic cracking reactions taking place inside the shape-selective zeolite, leading to increased ethylene content in the dry gas.

Influence of Design Margin on Operation Optimization and Control Performance of Chemical Processes

Operation optimization is an effective method to explore potential economic benefits for existing plants. The m.aximum potential benefit from operationoptimization is determined by the distances between current operating point and process constraints, which is related to the margins of design variables. Because of various ciisturbances in chemical processes, some distances must be reserved for fluctuations of process variables and the optimum operating point is not on some process constraints. Thus the benefit of steady-state optimization can not be fully achied（ed while that of dynamic optimization can be really achieved. In this study, the steady-state optimizationand dynamic optimization are used, and the potential benefit-is divided into achievable benefit for profit and unachievable benefit for control. The fluid catalytic cracking unit （FCCU） is used for case study. With the analysis on how the margins of design variables influence the economic benefit and control performance, the bottlenecks of process design are found and appropriate control structure can be selected.

Dynamic rupture and crushing of an extruded tube using artificial neural network(ANN)approximation method

A numerical study of the crushing of thin-walled circular aluminum tubes has been carried out to investigate the crashworthiness behaviors under axial impact loading. These kinds of tubes are usually used in automobile and train structures to absorb the impact energy. Previous researches show that thin-walled circular tube has the highest energy absorption under axial impact amongst different structures. In this work, the crushing between two rigid flat plates and the tube rupture by 4 and 6 blades cutting tools is modeled with the help of ductile failure criterion using the numerical method. The tube material is aluminum EN AW-7108 T6 and its length and diameter are 300 mm and 50 ram, respectively. Using the artificial neural network （ANN）, the most important surfaces of energy absorption parameters, including the maximum displacement of the striker, the maximum axial force, the specific energy absorption and the crushing force efficiency in terms of impact velocity and tube thickness are obtained and compared to each other. The analyses show that the tube rupture by the 6 blades cutting tool has more energy absorption in comparison with others. Furthermore, the results demonstrate that tube cutting with the help of multi-blades cutting tools is more stable, controllable and predictable than tube folding.

Tripartite Splitting Arbitrary 2-Qubit Quantum Information by Using Two Asymmetric W States

In this paper we propose a tripartite scheme for splitting an arbitrary 2-qubit quantum information by using two asymmetric W states as the quantum channel. In the schemem if the two recipients collaborate together, they can deterministically recover the quantum information by performing first a 4-qubit collective unitary operation and then two single-qubit unitary operations. In addition, since the asymmetric W states are employed as the quantum channel, the scheme is robust against decoherence.

Shale gas exploitation with supercritical CO_2 technology

This paper analyzes the physicochemical properties of supercritical C02, the characteristic of shale gas and shale gas reservoirs. The technologies of drilling, production, fracturing using the supercritical CO2 in shale gas explo- ration are proposed, to increase the penetration rate, decrease the damage to formation while fracturing, and enhance the recovery of shale gas. It is believed that the huge economic benefits of shale gas exploration with the supercritical CO2 fluid will be obtained, and it also can initiate a new technology field of CO2 in the petroleum engineering.

Successful Application of Hydrocracking Technology Aimed at Prodigiously Boosting Jet Fuel Yield

Recently ＂the hydrocarcking technology aimed at prodigiously boosting jet fuel yield along with improvement of tail-oil quality＂developed by the SINOPEC Research Institute of Petroleum Processing（RIPP） has been successfully applied in commercial scale on the 2.0 Mt/a hydrocracking unit at the SINOPEC Yanshan Branch Company, resulting in implementation of triple functions, viz.： boosting the jet fuel yield, reducing the diesel fuel output and improving the tail-oil quality. This technique has brought about obvious economic and environmental benefts, which can provide a mate-rial basis to bridge the demand gap in jet fuel supply at the new Beijing Airport and will serve as a good ex-ample of SINOPEC’s efforts in the area of transforma-tion of production mode, structure adjustment, product quality upgrading and enhancement of economic ben-efts at the refning enterprise.

Modeling and optimum operating conditions for FCCU using artificial neural network

A self-organizing radial basis function(RBF) neural network(SODM-RBFNN) was presented for predicting the production yields and operating optimization. Gradient descent algorithm was used to optimize the widths of RBF neural network with the initial parameters obtained by k-means learning method. During the iteration procedure of the algorithm, the centers of the neural network were optimized by using the gradient method with these optimized width values. The computational efficiency was maintained by using the multi-threading technique. SODM-RBFNN consists of two RBF neural network models: one is a running model used to predict the product yields of fluid catalytic cracking unit(FCCU) and optimize its operating parameters; the other is a learning model applied to construct or correct a RBF neural network. The running model can be updated by the learning model according to an accuracy criterion. The simulation results of a five-lump kinetic model exhibit its accuracy and generalization capabilities, and practical application in FCCU illustrates its effectiveness.

Granular cell tumor of the common bile duct: A Japanese case

Granular cell tumor (GCT) of the biliary system is rare. It is reported that it occurs more commonly in young black women. We report here our seldom experience of a Japanese case in whom icterus was found as a first symptom just after a caesarean operation. A 36-year-old Japanese woman developed icterus after delivery by the Caesarean operation. A surgical operation was performed without can deny that there was a tumor-related change in a bile duct as a result of examination for various images. As a result of pathological evaluation, GCT was diagnosed. By the preoperative organization biomicroscopy result, it was not able to be attachd a right diagnosis. It was thought that this tumor, although rare, should be considered as one of the causes of biliary stenosis in the younger population.

Catalytic Cracking and PSO-RBF Neural Network Model of FCC Cycle Oil

Catalytic cracking experiments of FCC cycle oil were carried out in a fixed fluidized bed reactor. Effects of reac- tion conditions, such as temperature, catalyst to oil ratio and weight hourly space velocity, were investigated. Hydrocarbon composition of gasoline was analyzed by gas chromatograph. Experimental results showed that conversion of cycle oil was low on account of its poor crackability performance, and the effect of reaction conditions on gasoline yield was obvi- ous. The paraffin content was very high in gasoline. Based on the experimental yields under different reaction conditions, a model for prediction of gasoline and diesel yields was established by radial basis function neural network （RBFNN）. In the model, the product yield was viewed as function of reaction conditions. Particle swarm optimization （PSO） algorithm with global search capability was used to obtain optimal conditions for a highest yield of light oil. The results showed that the yield of gasoline and diesel predicted by RBF neural network agreed well with the experimental values. The optimized reac- tion conditions were obtained at a reaction temperature of around 520 ~C, a catalyst to oil ratio of 7.4 and a space velocity of 8 h~. The predicted total yield of gasoline and diesel reached 42.2% under optimized conditions.

Parametric optimization of friction stir welding process of age hardenable aluminum alloys-ANFIS modeling

A comparative approach was performed between the response surface method(RSM) and the adaptive neuro-fuzzy inference system(ANFIS) to enhance the tensile properties, including the ultimate tensile strength and the tensile elongation, of friction stir welded age hardenable AA6061 and AA2024 aluminum alloys. The effects of the welding parameters, namely the tool rotational speed, welding speed, axial load and pin profile, on the ultimate tensile strength and the tensile elongation were analyzed using a three-level, four-factor Box-Behnken experimental design. The developed design was utilized to train the ANFIS models. The predictive capabilities of RSM and ANFIS were compared based on the root mean square error, the mean absolute error, and the correlation coefficient based on the obtained data set. The results demonstrate that the developed ANFIS models are more effective than the RSM model.

Preliminary Research on the Characteristics of the M_S8.0 Wenchuan Earthquake Hazard

The Ms8.0 Wenchuan earthquake on May 12, 2008 caused heavy casualties and economic loss. According to the field investigations, the characteristics can be described as follows： The meizoseismai region with an intensity of XI shows an obviously zonal distribution and suffered serious destruction from the earthquake, and the destruction perpendicular to the earthquake surface rupture decreased sharply. At the same time, the intensity X and IX regions perpendicular to the rupture are narrow and therefore their coverage area is small. The intensity on both sides of the rupture attenuates rapidly, but intensity VII and the VI regions are wide, the latter covering about 240,000 km^2. In intensity VI region, the damage area perpendicular to the rupture in the southern part is much larger than that of the northern part. Also, much new understanding about destruction types and destructive modes for all kinds of buildings, landforms and terrain is achieved in this paper.

Dual Isoflurane-induced Preconditioning Improves Neuroprotection in Rat Brain In Vitro and the Role of Extracellular Signal-regulated Protein Kinase

Objective To test the ability of isoflurane-induced preconditioning against oxygen and glucose dep- rivation （OGD） injury in vitro. Methods Rat hippocampal slices were exposed to 1 volume percentage （vol%）, 2vo1% or 3vo1% isoflurane respectively for 20 minutes under normoxic conditions （95% O2/5% CO2） once or twice （12 slices in each group） before OGD, with 15-minute washout after each exposure. During OGD experiments, hippocampus slices were bathed with artificial cerebrospinal fluid （ACSF） lacking glucose and perfused with 95% N2 and 5% CO2 for 14 minutes, followed by a 30-minute reperfusion in normal ACSF. The CA1 population spike （PS） was measured and used to quantify the degree of neuronal function recovery after OGD. To assess the role of mitogen-activated protein kinases （MAPKs） in isoflurane preconditioning, U0126, an inhibitor of extracellular signal-regulated protein kinase （ERK1/2）, and SB203580, an inhibitor of p38 MAPK, were used before two periods of 3vol% isoflurane exposure. Results The degree of neuronal function recovery of hippocampal slices exposed to 1 vol%, 2vol%, or 3vol% isoflurane once was 41.88%±9.23%, 55.05%±11.02%, or 63.18%±10.82% respectively. Moreover, neuronal function recovery of hippocampal slices exposed to 1 vol%, 2vo1%, or 3vo1% isoflurane twice was 53.75%±12.04%, 63.50%±11.06%, or 76.25%±12.25%, respectively. Isoflurane preconditioning increased the neuronal function recovery in a dose-dependent manner. U0126 blocked the preconditioning induced by dual exposure to 3vo1% isoflurane （6.13%±1.56%, P〈0.01） and ERK1/2 activities. Conclusions Isoflurane is capable of inducing preconditioning in hippocampal slices in vitro in a dose-dependent manner, and dual exposure to isoflurane with a lower concentration is more effective in triggering preconditioning than a single exposure. Isoflurane-induced neuroprotection might be involved with ERK 1/2 activities.