CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present,among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot,im...CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present,among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot,immunoelectron microscopy and antibody treatment with an anti-PSTAIRE monoclonal antibody to explore the subcellular localization and functions of PSTAIRE CDKs in Physarum polycephalum. The results of Western blot and immunoelectron microscopy showed that in P. polycephalum, a PSTAIRE CDK-like protein was 34 kD in molecular weight and located in both nuclei and cytoplasm. In nuclei, the protein was mainly associated with chromosomes and nucleoli. The expression of the PSTAIRE CDK-like protein in both the plasmodia and nuclei showed little fluctuation through the whole cell cycle. When treated with an anti-PSTAIRE monoclonal antibody at early S phase, the cells were arrested in S phase, and the mitotic onset of P. polycephalum was blocked for about 1 h when treated at early G2 phase.Our data indicated that the PSTAIRE CDK- like protein has a direct bearing on the mitosis.展开更多
In a changing world with a high interest in new ecomaterials, natural fibers such as the steelwool or Luffa cylindriea appear in new presentations and mixing with other materials to develop better opportunities to rep...In a changing world with a high interest in new ecomaterials, natural fibers such as the steelwool or Luffa cylindriea appear in new presentations and mixing with other materials to develop better opportunities to replace synthetic fibers. This work presents a research on the use of steelwool fiber for composite materials, on the basis of the physical properties, that generate conditions of mixing with three binders （matrix） natural： the rosin, artificial hard： polyurethane resin and artificial flexible： flexible twin resin （epoxy） and finally a polyester resin to make a comparison with other major resins. A testing of compression and tension is carried out to the materials analyzed, obtaining three types of composite materials by the above mentioned binders and three proposed presentations of fiber （complete, tissue and ground）. The test tube in tension with polyester resin presented a high rigidity and a percentage of deformation of 14%, resulting in less distortion than the woven with resin polyurethane with 12% of deformation. The presentation with resin polyurethane presented greater resistance to compression, because the resin acts as a sponge absorbing the energy of charge and the join of particles is larger than the other presentations generating greater cohesion among them and avoiding its rupture easily for a load of 2,000 kg. The presentation that is least resisted was the woven in two resins in the stage of energy absorption of load where polyurethane is 800 kg and the flexible twin is 850 kg, because the form of woven fiber distribution creates spaces where there are more resin than fiber.展开更多
This paper studies rotor spinning blended yam produced of spun silk and cashmere. Nine samples were spun, from three different opening rollers and different navels. According to the Uster test results of yam quality p...This paper studies rotor spinning blended yam produced of spun silk and cashmere. Nine samples were spun, from three different opening rollers and different navels. According to the Uster test results of yam quality properties, optimum selection is done by using analysis method of combining fuzzy decision-making and fuzzy pattern classification. Experimental plan is designed based on universal rotated experimental design, and the method of confined optimization is used to optimize the speed of opening roller, the speed of rotor and twist factor, according to the results of yam quality test by Uster.展开更多
Wool & silk blended fancy suiting is desinged. Through trial-production with silk sliver and Australian wool top, the spinning technology is investigated, and the relationship of spinning technology, blending rati...Wool & silk blended fancy suiting is desinged. Through trial-production with silk sliver and Australian wool top, the spinning technology is investigated, and the relationship of spinning technology, blending ratio and yarn construction is discussed.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.39870389)the Supported by the Excellent Young Teachers Program of MOE,P.R.C.
文摘CDKs play key roles in controlling cell cycle progression in all eukaryotes. In plants, multiple CDKs are present,among which the best characterized CDKs are PSTAIRE CDKs. In this study, we carried out Western blot,immunoelectron microscopy and antibody treatment with an anti-PSTAIRE monoclonal antibody to explore the subcellular localization and functions of PSTAIRE CDKs in Physarum polycephalum. The results of Western blot and immunoelectron microscopy showed that in P. polycephalum, a PSTAIRE CDK-like protein was 34 kD in molecular weight and located in both nuclei and cytoplasm. In nuclei, the protein was mainly associated with chromosomes and nucleoli. The expression of the PSTAIRE CDK-like protein in both the plasmodia and nuclei showed little fluctuation through the whole cell cycle. When treated with an anti-PSTAIRE monoclonal antibody at early S phase, the cells were arrested in S phase, and the mitotic onset of P. polycephalum was blocked for about 1 h when treated at early G2 phase.Our data indicated that the PSTAIRE CDK- like protein has a direct bearing on the mitosis.
文摘In a changing world with a high interest in new ecomaterials, natural fibers such as the steelwool or Luffa cylindriea appear in new presentations and mixing with other materials to develop better opportunities to replace synthetic fibers. This work presents a research on the use of steelwool fiber for composite materials, on the basis of the physical properties, that generate conditions of mixing with three binders （matrix） natural： the rosin, artificial hard： polyurethane resin and artificial flexible： flexible twin resin （epoxy） and finally a polyester resin to make a comparison with other major resins. A testing of compression and tension is carried out to the materials analyzed, obtaining three types of composite materials by the above mentioned binders and three proposed presentations of fiber （complete, tissue and ground）. The test tube in tension with polyester resin presented a high rigidity and a percentage of deformation of 14%, resulting in less distortion than the woven with resin polyurethane with 12% of deformation. The presentation with resin polyurethane presented greater resistance to compression, because the resin acts as a sponge absorbing the energy of charge and the join of particles is larger than the other presentations generating greater cohesion among them and avoiding its rupture easily for a load of 2,000 kg. The presentation that is least resisted was the woven in two resins in the stage of energy absorption of load where polyurethane is 800 kg and the flexible twin is 850 kg, because the form of woven fiber distribution creates spaces where there are more resin than fiber.
文摘This paper studies rotor spinning blended yam produced of spun silk and cashmere. Nine samples were spun, from three different opening rollers and different navels. According to the Uster test results of yam quality properties, optimum selection is done by using analysis method of combining fuzzy decision-making and fuzzy pattern classification. Experimental plan is designed based on universal rotated experimental design, and the method of confined optimization is used to optimize the speed of opening roller, the speed of rotor and twist factor, according to the results of yam quality test by Uster.
文摘Wool & silk blended fancy suiting is desinged. Through trial-production with silk sliver and Australian wool top, the spinning technology is investigated, and the relationship of spinning technology, blending ratio and yarn construction is discussed.
