尿糖、尿微量白蛋白结合检验对糖尿病早期肾损伤的诊断分析

目的研究糖尿病早期肾损伤诊断中采用尿糖+尿微量白蛋白检验的作用效果。方法选择该院2020年10月—2021年10月收治的100例糖尿病患者进行早期肾损伤诊断研究,采用回顾分析法,将患者分为早期肾损伤组(50例)与未损伤组(50例),观察尿糖检验、尿微量白蛋白检验、结合检验的组间差异情况以及数据分析结果。结果早期肾损伤组与未损伤组尿糖、尿微量白蛋白相比,差异有统计学意义(P<0.05);尿糖检验结果与尿微量白蛋白检验结果相比,差异无统计学意义(P>0.05);尿糖检验与结合检验相比,差异有统计学意义(P<0.05);结合检验与尿微量白蛋白检验相比,差异有统计学意义(P<0.05);结合检验真阳性率高于尿糖检验、尿微量白蛋白检验,真阴性、假阳性数据均一致,假阴性数据低于尿糖检验、尿微量白蛋白检验;结合检验敏感度、准确性、阴性预测值、正确指数均高于尿糖检验、尿微量白蛋白检验,特异度、误诊率、阳性预测值、假阳性率均一致,漏诊率、假阴性率低于尿糖检验、尿微量白蛋白检验。结论早期糖尿病肾损伤患者尿糖、尿微量白蛋白有明显的差异变化,采用尿糖+尿微量白蛋白结合检验方式可以提升糖尿病早期损伤阳性诊断率。

浅谈棉花手扯长度的检验方法

分析了影响棉花纤维长度的因素,并介绍了棉花手扯长度三种检验方法。为提高手扯长度的准确性,笔者建议采用"三结合"检验法,并经常校验个人的手法,以得到一个修正系数,达到准确、快速测试的效果。

药品化妆品抽样及检验的有关问题探讨

目的:对药品、药包材、化妆品抽样及检验工作中发现的问题进行分析研究,旨在为监管部门和检验机构提供参考,规范抽样和检验,提高监管效能和服务水平。方法:通过抽样、样品寄送、收样、检验等环节,对药品、药包材、化妆品抽样及检验工作中发现的有关问题进行分析研究,提出相应的解决措施和意见建议。结果与结论:部分药品/药包材/化妆品的抽查检验存在抽样不规范、抽样信息不完整、抽样与检查未能兼顾、样品寄送不规范、检验登记单和检验报告信息不完整或填写不规范、检验不规范、部分产品的批号效期标识不规范,以及部分品种因抽样量大导致抽样难以覆盖基层等问题,影响了监管效能。应根据药品、药包材、化妆品的产品特性和监管要求,分类设置并完善抽样及检验检测信息,并从强化人员培训、监督和检验有机结合、针对基层用药特点完善抽检工作机制、加强学习交流提高检验报告质量等方面加以解决。

血常规结合凝血六项检验在孕产妇临床妊娠期间应用价值分析

分析在孕产妇临床妊娠期间使用血常规结合凝血六项检验所发挥的应用价值。方法 选取我院检验科一年间(2022年1月至2022年12月)妊娠期孕产妇(1100例)进行研究,按照妊娠时期将其分为四组(每组均包括275例孕产妇):孕早期(妊娠1~12周)、孕中期(妊娠13~27周)、孕晚期(妊娠28~40周)与临产期。对四组孕产妇都给予血常规结合凝血六项检验并进行组间的数据对比,旨在分析上述检验项目对于孕产妇临床妊娠期间的意义和影响。结果 对四组不同时期的孕产妇进行分析发现,在产妇临床妊娠期间,伴随孕周增加,产妇的体重也呈现出上升的趋势,差异显著(P<0.05)。此外,随着孕周的增加,WBC、RDW水平随之上升,而MCV水平随之下降,差异显著(P<0.05),但PLT水平在妊娠期间差异不显著(P>0.05)。随着孕周的增加,FIB、D-D水平随之上升,而APTT、TT、AT-III水平随之下降,差异显著(P<0.05),但PT水平在妊娠期间差异不显著(P>0.05)。高凝孕产妇的并发症发生率(10.71%)高于低凝孕产妇(2.22%),差异显著(P<0.05)。结论 血常规结合凝血六项检验在孕产妇临床妊娠期间的效果良好,能够帮助对妊娠合并症进行有效判断,保障母婴安全,值得推广。

乙型肝炎病毒免疫检测弱反应性标本的产生原因及处理

我国是乙型肝炎（type B hepatitis,HB）疾病的大国,而乙型肝炎病毒（hepatitis B virus,HBV）免疫检测对于HBV感染者的诊断和治疗具有特殊的意义,如何准确、及时的对HBV感染者血液标本进行检测并合理发出报告就显得至关重要了。近年来，HBV免疫检测的最大问题就是假阴性、假阳性问题。大多是因为弱反应性标本所引起的，而国内对HBV免疫检测弱反应性标本的处理常采取按照试剂盒的临界值定标，硬性判断并报告阴、阳性结果。

DETECTION OF B LYMPHOMA CELLS UNDERGOING APOPTOSIS BY ANNEXIN-V ASSAY

To quantitatively analyze apoptotic and secondary necrotic cells unde r apoptosis conditions. Methods. The cells of Burkitt lymphoma (BL) cell line Raji were incubated with 1 .0 μmol/L dexamethasone (DEX) for 2, 4 and 8 h respectively, then stained with Annexin V FITC (fluorescein isothiocyanate conjugated) which was used to detec t the exposed phosphatidylserine (PS) on the epimembrane resulting from a loss o f phospholipid asymmetry in the early stage of apoptosis, and also stained with propidium iodide (PI) which allows analysis of secondary necrotic cells related with cell membrane and DNA damage that probably represent late stage of apoptosi s, then apoptotic cells were quantified by flow cytometry (FCM). Furthermore, An nexin+/PI and Annexin+/PI+ cells were sorted by fluoresence activated cell sorter (FACS), and identified by electron microscopy (EM) and DNA gel electroph oresis. Results. The percentage of apoptotic cells was found to increase with the incuba tion time (r=0.97). This method was sensitive with low detection limit (0.02%), and was reproducible with low coefficient variance (CV)(4.2%). Meanwhile, the Annexin+/PI and Annexin+/PI+ cells were identified as apoptotic and necroti c cells under EM, and DNA extracted from the Annexin+/PI cells was characteri zed by “ladder pattern”. Conclusions. Annexin V assay is a specific, sensitive, accurate, reproductive and quantitative method for analyzing apoptotic cells.

^(14)C-urea breath test in patients undergoing anti-tuberculosis therapy

AIM: Urea breath test (UBT) is a non-invasive diagnostic test for detecting the presence of Helicobacter pylori(H pylori).In this study we evaluated the effect of anti-tuberculosis therapy on the results of 14C-UBT.METHODS: Patients, with the diagnosis of tuberculosis (TB) who had a positive UBT at the point of starting antiTB therapy, were included. None had a history of peptic ulcer disease or had taken antibiotics, bismuth compounds and/or PPI in the previous month, 14C-UBT was repeated at the end of the second month and the end of treatment period and one month after completion of treatment course.RESULTS: Thirty-five patients (23 males) were enrolled.14C-UBT was negative in all 35 patients (100%) at the end of the second month and remained negative in 30cases (85.7%) at the end of the treatment course. One month after completion of treatment course, UBT remained negative in 13 patients (37.1%).CONCLUSION: Our report underscores the need for caution while interpreting urea breath test results in patients undergoing anti-TB therapy. Furthermore, the combination of drugs used in this study resulted in H pylori eradication in a minority of patients.

Is There Hysteresis in Unemployment in OECD Countries？ Evidence From Panel Unit Root Test With Structural Breaks

This study tests the hysteresis hypothesis of unemployment in fifteen OECD countries by using panel unit root tests which allow for structural breaks. We apply annual unemployment rates covering 1985-2008 periods. We test whether unemployment rates are stationary by using second generation tests which allow cross section dependency among series and panel unit root test based on structural break advanced by Carrion-i-Silvestre, Barrio-Castro and Lopez-Bazo （2005）. We find series as a stationary process with structural breaks according to Carrion-i Silvestre et al. （2005） test, while we find series as unit root process with second generation panel unit root test. According to the Carrion-i Silvestre et al. （2005） test, we find the evidence of absence of hysteresis in analyzed countries. As a result, temporary shocks have temporary effects on unemployment instead of permanent effect. Structural factors can affect the natural rate of unemployment and, therefore, unemployment would be stationary around a process that is subject to structural breaks. So, there still exists a unique natural rate of unemployment to which the economy eventually will converge.