人脂肪细胞新乙二醛酶的发现及结构功能分析

目的通过蛋白组学技术筛选人脂肪细胞新蛋白,并对新蛋白的结构及功能进行分析。方法利用双向电泳及质谱技术观察人脂肪细胞蛋白表达谱,对发现的新蛋白先通过RT-PCR及原核表达证实该蛋白在脂肪细胞的表达,再利用生物信息学对新蛋白的结构,同源性及结构域进行预测。结果发现了脂肪细胞表达的新蛋白My027,证实了该蛋白的表达,生物信息学分析显示该蛋白定位于胞浆,具有高度保守的结构域。141-253氨基酸之间有一个完整的乙二醛酶(glyoxalse,GLO)功能域,与GLO的同源性为96.7%,144-244氨基酸序列与GLO I的同源性为95.7%。结论脂肪细胞表达的蛋白My027具有与GLO I基本相同的功能域,很可能具有同样的生理功能。

Analysis of Emergy Structure,Function and Efficiency of Agro-ecosystem in Hunan Province

[Objective] The aim was to explore function and efficiency of emergy input/ output of Hunan agricultural ecosystem to reveal relationship of human being with natural resources and environment. [Method] Emergy structure, function and efficien- cy of Hunan agricultural ecosystem were analyzed based on input/output data in Hunan Province in 2009 as per emergy theory. [Result] The structure characters of agricultural ecosystem were as follows： Hunan is characterized with traditional agri- culture, depending greatly on both human and animal labor. For industrial accessory emergy, chemical fertilizers make most contribution to the system, and agricultural mechanization is not satisfied. Furthermore, renewable ratio of industrial accessory emergy is lower, and development and potential of green energy is promising. In addition, prices of the products are lower, without consideration of contribution made by natural resources and environment. It also suggested that Hunan agricultural chain is short and added value of products is not high. In general, emergy output of farming and animal husbandry dominates and the prices are lower than those of forestry and fishery. The function of the system was as follows： With lower environ- mental load ratio, the system is overloaded by population and the index of sustain- able development was 5.96, suggesting that the system enjoys vitality and potential, but the economy is undeveloped and the pressure from natural resources and envi- ronment is not high. The emergy output ratio was a little lower than national level in 2009, suggesting Hunan agricultural production is extensive. [Conclusion] The re- search indicated that rapid increase of population should be controlled; surplus labor should be transferred; agricultural structure and products structure should be further adjusted; agricultural technology should be further developed; agdcultural mechaniza- tion and modernization should be improved.

Analysis of the Protein Structure and Function of HpaG_(Xoo)

[Objective] The study was to analyze the structure and function of HpaGXoo and the relationship between the two.[Method] Some related bioinformatics analysis software on internet such as NPSA,Swiss-Model,SAPS and InterPro Scan were adopted to analyze the structure and predict its function.[Result] HpaGXoo consists of 139 amino acids,and has many alpha-helical and coiled structure,no signal peptide on N-terminal and no transmembrane structure.It locates in bacterial cytoplasm.[Conclusion] The study will lay ...

Characteristics of soil microbial community functional and structure diversity with coverage of Solidago Canadensis L

The relationship between Solidago canadensis L. invasion and soil microbial community diversity including functional and structure diversities was studied across the invasive gradients varying from 0 to 40%, 80%, and 100% coverage of Solidago canadensis L. using sole carbon source utilization profiles analyses, principle component analysis （PCA） and phospholipid fatty acids （PLFA） profiles analyses. The results show the characteristics of soil microbial community functional and structure diversity in invaded soils strongly changed by Solidago canadensis L. invasion. Solidago canadensis L. invasion tended to result in higher substrate richness, and functional diversity. As compared to the native and ecotones, average utilization of specific substrate guilds of soil microbe was the highest in Solidago canadensis L. monoculture. Soil microbial functional diversity in Solidago canadensis L. monoculture was distinctly separated from the native area and the ecotones. Aerobic bacteria, fungi and actinomycetes population significantly increased but anaerobic bacteria decreased in the soil with Solidago canadensis L. monoculture. The ratio of cyl9：0 to 18：1 co7 gradually declined but mono/sat and fung/bact PLFAs increased when Solidago canadensis L. became more dominant. The microbial community composition clearly separated the native soil from the invaded soils by PCA analysis, especially 18： lco7c, 16： lco7t, 16： lco5c and 18：2co6, 9 were present in higher concentrations for exotic soil. In conclusion, Solidago canadensis L. invasion could create better soil conditions by improving soil microbial community structure and functional diversity, which in turn was more conducive to the growth ofSolidago canadensis L.

Geochip-based analysis of microbial functional genes diversity in rutile bio-desilication reactor

Biological desilication process is an effective way to remove silicate from rutile so that high purity rutile could be obtained. However, little is known about the molecular mechanism of this process. In this work, a newly developed rutile bio-desilication reactor was applied to enrich rutile from rough rutile concentrate obtained from Nanzhao rutile mine and a comprehensive high through-put functional gene array(Geo Chip 4.0) was used to analyze the functional gene diversity, structure and metabolic potential of microbial communities in the biological desilication reactor. The results show that TiO2 grade of the rutile concentrate could increase from 78.21% to above 90% and the recovery rate could reach to 96% or more in 8-12 d. The results also show that almost all the key functional genes involved in the geochemical cycling process, totally 4324 and 4983 functional microorganism genes, are detected in the liquid and ore surface, respectively. There are totally 712 and 831 functional genes involved in nitrogen cycling for liquid and ore surface samples, respectively. The relative abundance of functional genes involved in the phosphorus and sulfur cycling is higher in the ore surface than liquid. These results indicate that nitrogen, phosphorus and sulfur cycling are also present in the desiliconization process of rutile. Acetogenesis genes are detected in the liquid and ore surface, which indicates that the desiliconizing process mainly depends on the function of acetic acid and other organic acids. Four silicon transporting genes are also detected in the sample, which proves that the bacteria have the potential to transfer silicon in the molecule level. It is shown that bio-desilication is an effective and environmental-friendly way for enrichment of rough rutile concentrate and presents an overview of functional diversity and structure of desilication microbial communities, which also provides insights into our understanding of metabolic potential in biological desilication reactor ecosystems.

Numerical analysis and experimental investigation of wind turbine blades with innovative features:Structural response and characteristics

Innovative features of wind turbine blades with flatback at inboard region,thick airfoils at inboard as well as mid-span region and transversely stepped thickness in spar caps have been proposed by Institute of Engineering Thermophysics,Chinese Academy of Sciences(IET-Wind)in order to improve both aerodynamic and structural efficiency of rotor blades.To verify the proposed design concepts,this study first presented numerical analysis using finite element method to clarify the effect of flatback on local buckling strength of the inboard region.Blade models with various loading cases,inboard configurations,and core materials were comparatively studied.Furthermore,a prototype blade incorporated with innovative features was manufactured and tested under static bending loads to investigate its structural response and characteristics.It was found that rotor blades with flatback exhibited favorable local buckling strength at the inboard region compared with those with conventional sharp trailing edge when low-density PVC foam was used.The prototype blade showed linear behavior under extreme loads in spar caps,aft panels,shear web and flatback near the maximum chord which is usually susceptible to buckling in the blades according to traditional designs.The inboard region of the blade showed exceptional load-carrying capacity as it survived420%extreme loads in the experiment.Through this study,potential structural advantages by applying proposed structural features to large composite blades of multi-megawatt wind turbines were addressed.