The precise design and fabrication of biomaterial scaffolds is necessary to provide a systematic study for bone tissue engineering. Biomaterial scaffolds should have sufficient stiffness and large porosity. These two ...The precise design and fabrication of biomaterial scaffolds is necessary to provide a systematic study for bone tissue engineering. Biomaterial scaffolds should have sufficient stiffness and large porosity. These two goals generally contradict since larger porosity results in lower mechanical properties. To seek the microstructure of maximum stiffness with the constraint of volume fraction by topology optimization method, algorithms and programs were built to obtain 2D and 3D optimized microstructure and then they were transferred to CAD models of STL format. Ti scaffolds with 30% volume fraction were fabricated using a selective laser melting （SLM） technology. The architecture and pore shape in the metallic biomaterial scaffolds were relatively precise reproduced and the minimum mean pore size was 231μm. The accurate fabrication of intricate microstructure has verified that the SLM process is suitable for fabrication of metallic biomaterial scaffolds.展开更多
Construction of functional porous titanium scaffold is drawing ever growing attention, due to its effectiveness in solving the mechanical mismatch between titanium implant and bone tissue. However, the poor water perm...Construction of functional porous titanium scaffold is drawing ever growing attention, due to its effectiveness in solving the mechanical mismatch between titanium implant and bone tissue. However, the poor water permeability as well as the problem in achieving uniform surface modification inside scaffold hinders the further biomedical application of porous titanium scaffold. In this study, largescale functional Ti O2 nanostructures（nanonetwork, nanoplate and nanowire） were constructed on three-dimensional porous titanium scaffolds surface via an effective hydrothermal treatment method. These nanostructures increase the hydrophilicity of the titanium scaffold surface, facilitating the cell culture medium to penetrate into the inner pore of the scaffold. Zeta potential analyses indicate that the surface electrical properties depend on the nanostructure, with nanowire exhibiting the lowest potential at p H 7.4. The influence of the nano-functionalized scaffold on protein adsorption and cell adhesion was examined. The results indicate that the nano-functionalized surface could modulate protein adsorption and bone marrow derived mesenchymal stem cells（BMSCs） adhesion, with the nanowire functionalized porous scaffold homogeneously promoting protein adsorption and BMSCs adhesion. Our research will facilitate future research on the development of novel functional porous scaffold.展开更多
基金Project (51275179) supported by the National Natural Science Foundation of ChinaProject (2010A090200072) supported by Industry,University and Research Institute Combination of Ministry of Education, Ministry of Science and Technology and Guangdong Province,China+1 种基金Project (2012M511797) supported by China Postdoctoral Science FoundationProject (2012ZB0014) supported by FundamentalResearch Funds for the Central Universities of China
文摘The precise design and fabrication of biomaterial scaffolds is necessary to provide a systematic study for bone tissue engineering. Biomaterial scaffolds should have sufficient stiffness and large porosity. These two goals generally contradict since larger porosity results in lower mechanical properties. To seek the microstructure of maximum stiffness with the constraint of volume fraction by topology optimization method, algorithms and programs were built to obtain 2D and 3D optimized microstructure and then they were transferred to CAD models of STL format. Ti scaffolds with 30% volume fraction were fabricated using a selective laser melting （SLM） technology. The architecture and pore shape in the metallic biomaterial scaffolds were relatively precise reproduced and the minimum mean pore size was 231μm. The accurate fabrication of intricate microstructure has verified that the SLM process is suitable for fabrication of metallic biomaterial scaffolds.
基金supported by the National High Technology Research and Development Program of China(2015AA033502)the National Natural Science Foundation of China(51372087,51232002 and 51541201)+3 种基金the Science and Technology Planning Project of Guangdong Province,China(2014A010105048)the Natural Science Foundation of Guangdong Province(2015A030313493 and 2016A030308014)the State Key Laboratory for Mechanical Behavior of Materials,China(20141607)the Technological Projects of Guangzhou,China(201604020110)
文摘Construction of functional porous titanium scaffold is drawing ever growing attention, due to its effectiveness in solving the mechanical mismatch between titanium implant and bone tissue. However, the poor water permeability as well as the problem in achieving uniform surface modification inside scaffold hinders the further biomedical application of porous titanium scaffold. In this study, largescale functional Ti O2 nanostructures（nanonetwork, nanoplate and nanowire） were constructed on three-dimensional porous titanium scaffolds surface via an effective hydrothermal treatment method. These nanostructures increase the hydrophilicity of the titanium scaffold surface, facilitating the cell culture medium to penetrate into the inner pore of the scaffold. Zeta potential analyses indicate that the surface electrical properties depend on the nanostructure, with nanowire exhibiting the lowest potential at p H 7.4. The influence of the nano-functionalized scaffold on protein adsorption and cell adhesion was examined. The results indicate that the nano-functionalized surface could modulate protein adsorption and bone marrow derived mesenchymal stem cells（BMSCs） adhesion, with the nanowire functionalized porous scaffold homogeneously promoting protein adsorption and BMSCs adhesion. Our research will facilitate future research on the development of novel functional porous scaffold.
