采用RT-PCR原理和长片段扩增技术克隆细角滨对虾酚氧化酶原基因。结果表明,细角滨对虾血淋巴细胞内存在2个pro PO基因。pro PO gene 1的c DNA序列包含有372氨基酸,前190个氨基酸为一个M家族血蓝蛋白,是一个铜结合位点区域,191-372为一个...采用RT-PCR原理和长片段扩增技术克隆细角滨对虾酚氧化酶原基因。结果表明,细角滨对虾血淋巴细胞内存在2个pro PO基因。pro PO gene 1的c DNA序列包含有372氨基酸,前190个氨基酸为一个M家族血蓝蛋白,是一个铜结合位点区域,191-372为一个C家族的血蓝蛋白,是一个免疫球蛋白样的区域。pro PO gene 2的2个功能位点之间的序列有重叠,pro PO gene 2 c DNA序列的6-935bp包含了第一个功能位点,928-1464bp则包含了第二个功能位点。系统进化树比对分析发现2个基因之间的序列差异非常大。细角滨对虾和凡纳滨对虾的pro PO gene 2同处于一个密切相关的群,pro PO gene 1则和其他几种对虾的pro PO gene处于一个群。pro PO gene 2与pro PO gene 1在对虾免疫活动中是否存在不同的功能还有待于进一步的研究。展开更多
Aiming at the problem of on-line damage diagnosis in structural health monitoring （SHM）, an algorithm of feature extraction and damage alarming based on auto-regressive moving-average （ARMA） time series analysis i...Aiming at the problem of on-line damage diagnosis in structural health monitoring （SHM）, an algorithm of feature extraction and damage alarming based on auto-regressive moving-average （ARMA） time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.展开更多
Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein...Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mt genome of Gekko is similar to most of the vertebrates in gene components, order, orientation, tRNA structures, low percentage of guanine and high percentage of thymine, and skews of base GC and AT. Base A was preferred at third codon positions for protein genes is similar to amphibians and fishes rather than amnion vertebrates. The standard stop codes (TAA) present only in three protein genes, less than those of most vertebrates. Transfer RNA genes range in length from 63 to 76 nt, their planar structure present characteristic clover leaf, except for tRNA-Cys and tRNA-Ser (AGY) because of lacking the D arm.展开更多
Time series of wind speed are composed of large and small ramp structures. Data analysis reveals a power law relation between the linear slope of ramp structures and the time scale. This suggests that these ramp struc...Time series of wind speed are composed of large and small ramp structures. Data analysis reveals a power law relation between the linear slope of ramp structures and the time scale. This suggests that these ramp structures of wind speed have a self-similar characteristic. The lower limit of the self-similar scale range was 2 s. The upper limit is unexpectedly large at 27 rain. Data are collected from grassland, city, and lake areas. Although these data have different underlying surfaces, all of them clearly show a power law relation, with slight differences in their power exponents.展开更多
文摘采用RT-PCR原理和长片段扩增技术克隆细角滨对虾酚氧化酶原基因。结果表明,细角滨对虾血淋巴细胞内存在2个pro PO基因。pro PO gene 1的c DNA序列包含有372氨基酸,前190个氨基酸为一个M家族血蓝蛋白,是一个铜结合位点区域,191-372为一个C家族的血蓝蛋白,是一个免疫球蛋白样的区域。pro PO gene 2的2个功能位点之间的序列有重叠,pro PO gene 2 c DNA序列的6-935bp包含了第一个功能位点,928-1464bp则包含了第二个功能位点。系统进化树比对分析发现2个基因之间的序列差异非常大。细角滨对虾和凡纳滨对虾的pro PO gene 2同处于一个密切相关的群,pro PO gene 1则和其他几种对虾的pro PO gene处于一个群。pro PO gene 2与pro PO gene 1在对虾免疫活动中是否存在不同的功能还有待于进一步的研究。
基金The National High Technology Research and Devel-opment Program of China (863Program) (No2006AA04Z416)the National Natural Science Foundation of China (No50538020)
文摘Aiming at the problem of on-line damage diagnosis in structural health monitoring （SHM）, an algorithm of feature extraction and damage alarming based on auto-regressive moving-average （ARMA） time series analysis is presented. The monitoring data were first modeled as ARMA models, while a principalcomponent matrix derived from the AR coefficients of these models was utilized to establish the Mahalanobisdistance criterion functions. Then, a new damage-sensitive feature index DDSF is proposed. A hypothesis test involving the t-test method is further applied to obtain a decision of damage alarming as the mean value of DDSF had significantly changed after damage. The numerical results of a three-span-girder model shows that the defined index is sensitive to subtle structural damage, and the proposed algorithm can be applied to the on-line damage alarming in SHM.
文摘Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay (Gekko gecko). The genome is 16 435 bp in size, contains 13 protein-coding, 2 ribosomal and 22 transfer RNA genes. The mt genome of Gekko is similar to most of the vertebrates in gene components, order, orientation, tRNA structures, low percentage of guanine and high percentage of thymine, and skews of base GC and AT. Base A was preferred at third codon positions for protein genes is similar to amphibians and fishes rather than amnion vertebrates. The standard stop codes (TAA) present only in three protein genes, less than those of most vertebrates. Transfer RNA genes range in length from 63 to 76 nt, their planar structure present characteristic clover leaf, except for tRNA-Cys and tRNA-Ser (AGY) because of lacking the D arm.
基金supported by the National Natural Science Foundation of China (Grant No. 91215302)"One-Three-Five" Strategic Planning (wind power prediction) of the Institute of Atmospheric Physics, Chinese Academy of Sciences (CAS) (Grant No. Y267014601)the Strategic Project of Science and Technology of CAS (Grant No. XDA05040301)
文摘Time series of wind speed are composed of large and small ramp structures. Data analysis reveals a power law relation between the linear slope of ramp structures and the time scale. This suggests that these ramp structures of wind speed have a self-similar characteristic. The lower limit of the self-similar scale range was 2 s. The upper limit is unexpectedly large at 27 rain. Data are collected from grassland, city, and lake areas. Although these data have different underlying surfaces, all of them clearly show a power law relation, with slight differences in their power exponents.
