英语中的"双子星"结构的基本形式是"word A and word B",是英语中一种特殊的习语,在音、形、义及文体等方面具有鲜明的特点。根据词性及word A和word B的相互关系,可以把这种双子星结构分成多种类型。英语中的"...英语中的"双子星"结构的基本形式是"word A and word B",是英语中一种特殊的习语,在音、形、义及文体等方面具有鲜明的特点。根据词性及word A和word B的相互关系,可以把这种双子星结构分成多种类型。英语中的"双子星"结构具有意义上的统一性、结构上的稳定性、应用上的整体性等特点,并具有形象生动、言简意赅、寓意深刻、加强语气、语义等功能。展开更多
Antofloxacin free base is prepared from antofloxacin hydrochloride by removing hydrogen chloride. Its crystal is obtained by slow evaporation of an acetonitrile-methanol mixed solution. Single-crystal X-ray diffractio...Antofloxacin free base is prepared from antofloxacin hydrochloride by removing hydrogen chloride. Its crystal is obtained by slow evaporation of an acetonitrile-methanol mixed solution. Single-crystal X-ray diffraction reveals that the crystallography belongs to a triclinic PI space group with cell parameters: a = 0. 663 07(13) nm, b = 0. 898 39(18) nm, c = 1. 569 0(3) nm, α = 75.12(3)°,β = 87.92(3)°, γ = 77.57 (3)°. Antofloxacin shows no fluorescence in solution, but the crystalline state emits strong green light at 510 nm under the excitation of 360 nm, indicating a fluorescence enhancement induced by aggregation. It demonstrates that intermolecular packing and interaction in the crystal lead to the improved fluorescence quantum yield. These results provide important intbrmation for the further exploration of the structure-activity relationship of antofloxacin and the development of new drugs.展开更多
Although a growing number of both sequence-based and microsatellite nuclear loci have been used to infer genetic structures, their relative efficiencies remain poorly understood. In our study, we used the Green-backed...Although a growing number of both sequence-based and microsatellite nuclear loci have been used to infer genetic structures, their relative efficiencies remain poorly understood. In our study, we used the Green-backed Tit (Parus monticolus) to explore the resolving ability of these two types of markers. The south-western and central mitochondrial DNA (mtDNA) phylogroups were divergent to some extent in sequence-based nuclear data, while mixed together in microsatellites data. The F ST values among clades were about four times lower in microsatellite loci than those in sequence-based nuclear loci. We are of the opinion that size homoplasy may have contributed to the inability of microsatellites to uncover differentiation. Our results suggest that sequence-based nuclear loci outperformed microsatellite loci in detecting population structures, especially those focused on populations with large effective population sizes. There was no significant correlation between F ST values and allelic size variability, which suggested that the efficiency of microsatellite loci in detecting genetic structure may be independent of their polymorphism. F ST is better than R ST in detecting intraspecific divergence due to the high variance of R ST . In agreement with sequence-based nuclear loci, microsatellite loci did resolve the genetic distinctness of the Taiwan Residents phylogroup. The genetic differentiation between the Taiwan Residents and continental clades may involve allopatric divergence without gene flow.展开更多
In order to evaluate the practicality and effectiveness of the turn-based algorithm for logit loading (TALL), the TALL is implemented using C++, and it is compared with a combination of the network-expanding metho...In order to evaluate the practicality and effectiveness of the turn-based algorithm for logit loading (TALL), the TALL is implemented using C++, and it is compared with a combination of the network-expanding method and the Dial algorithm based on the analysis of algorithm procedures. The TALL uses the arc-labeling shortest path searching, bidirectional star and the deque structure to directly assign the traffic flow, while the Dial algorithm should be used in an expanded network. The test results over realistic networks of eight cities show the superior performance of the TALL algorithm over the combination of the network-expanding method and the Dial algorithm, and the average processing time is reduced by 55. 4%. Furthermore, it is found that the operational efficiency of the TALL relates to the original densities of the cities. The average processing time is reduced by 65. 1% when the original density is about 14%, but the advantage of the TALL is not obvious with the increase in the original density.展开更多
Monodisperse nanoparticle assembly with tunable structure, composition and properties can be taken as a superstructured building block for the construction of hierarchical nanostruc tures from the bottom up, which als...Monodisperse nanoparticle assembly with tunable structure, composition and properties can be taken as a superstructured building block for the construction of hierarchical nanostruc tures from the bottom up, which also represents a great challenge in nanotechnology. Here we report on a facile and controllable method that enables a high yield fabricatioa of uniform gold nanoparticle (AuNP) coresatellites with definable number (in average) of the satellite particles and tunable coretosatellite distance. The formation of the coresatellite nanostruc tures is driven by programmable DNAbasepairing, with the resulting nanocomplexes being isolatable via gel electrophoresis. By rationally controlling the DNA coverages on the core and shell particles, high production yields are achieved for the assembly/isolation process. As well, benefiting from a minimum DNA coverage on the satellite AuNPs, a strong affinity is observed for the asprepared coresatellites to get adsorbed on proteincoated graphene ox ide, which allows for a twodimensional hierarchical assembly of the coresatellite structures. The resulting hierarchical nanoassemblies are expected to find applications in various areas, including plasmonics, biosensing, and nanocatalysis. The method should be generalizable to make even more complicated and higherorder structures by making use of the structural programmability of DNA molecules.展开更多
Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To p...Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.展开更多
This paper is aimed to propose an approach to predict the dynamic characteristics of ring-plate planetary indexing cam mechanism, which is a novel type of indexing mechanism that employs internal planetary transmissio...This paper is aimed to propose an approach to predict the dynamic characteristics of ring-plate planetary indexing cam mechanism, which is a novel type of indexing mechanism that employs internal planetary transmission structure. Firstly, the geometry and structure of the mechanism are discussed and the kinematic practicability is simulated with virtual prototype design. Then a 3D finite element model of the ring-plate planetary indexing cam mechanism is developed with the commercial software of MATLAB and ANSYS. Through the finite element analysis, the natural frequencies and the corresponding mode shapes are predicted in one motion cycle. On the basis of the virtual prototype design and finite element analysis, an experimental prototype is made and tested to validate the prediction of the dynamic characteristics. The agreement between experimental results and the finite element analysis testifies that the finite element model developed is applicable to the prediction of the dynamic characteristics of this type of mechanism.展开更多
The Daurian Partridge(Perdix dauuricae) is a kind of hunting bird with high economic value.Genetic diversity and structure in the Daurian Partridge were studied by analyzing eight microsatellite loci in 23 populations...The Daurian Partridge(Perdix dauuricae) is a kind of hunting bird with high economic value.Genetic diversity and structure in the Daurian Partridge were studied by analyzing eight microsatellite loci in 23 populations found throughout the range of the species in China.The objectives were to evaluate the consequences on genetic diversity and differentiation of Daurian Partridge populations and to obtain a profound genetic insight for future management decisions and for effective measures to protect and exploit Daurian Partridges.The results showed that microsatellites were polymorphic in all Daurian Partridge populations,with a high level of genetic diversity over all the loci,especially in the Qaidam Basin populations which have the highest level of diversity.Significant genetic divergence was observed among different groups as well as between populations within the same group;most pairwise FST values were highly significant.Both phylogenetic trees and Bayesian clustering analyses revealed clear differentiation among the 23 populations of the Daurian Partridge,which were classified into two genetically differentiated groups.A bottleneck analysis indicated that Daurian Partridge populations have experienced a recent bottleneck.Our study argues that the Qaidam populations,North China populations,JN population,ZJC population,and Liupan Mountain populations should be paid special attention in order to retain adequate population sizes for maintaining genetic diversity.展开更多
The architecture of cislunar multi-hop communication networks, which focuses on the requirements of lunar full-coverage and continuous cislunar communications, is presented on the basis of Geosynchronous Orbit (GEO) s...The architecture of cislunar multi-hop communication networks, which focuses on the requirements of lunar full-coverage and continuous cislunar communications, is presented on the basis of Geosynchronous Orbit (GEO) satellite network relays. According to the geographical distribution of the forthcoming Chinese Deep Space Measuring and Controlling Network (DSMCN), two networking schemes are proposed and two elevation angle optimization models are established for locating GEO relay satellites. To analyze the dynamic connectivity, a dynamic network model is constructed with respect to the time-varying characteristics of cislunar trunk links. The advantages of the two proposed schemes, in terms of the Connectivity Rate (CR), Interruption Frequency (IF), and Average Length of Connecting Duration (ALCD), are corroborated by several simulations. In the case of the lunar polar orbit constellation case, the gains in the performance of scheme I are observed to be 134.55%, 117.03%, and 217.47% compared with DSMCN for three evaluation indicators, and the gains in the performance of scheme II are observed to be 238. 22%, 240.40%, and 572.71%. The results validate that the connectivity of GEO satellites outperforms that of earth facilities significantly and schemes based on GEO satellite relays are promising options for cislunar multi-hop communication networking.展开更多
文摘英语中的"双子星"结构的基本形式是"word A and word B",是英语中一种特殊的习语,在音、形、义及文体等方面具有鲜明的特点。根据词性及word A和word B的相互关系,可以把这种双子星结构分成多种类型。英语中的"双子星"结构具有意义上的统一性、结构上的稳定性、应用上的整体性等特点,并具有形象生动、言简意赅、寓意深刻、加强语气、语义等功能。
文摘Antofloxacin free base is prepared from antofloxacin hydrochloride by removing hydrogen chloride. Its crystal is obtained by slow evaporation of an acetonitrile-methanol mixed solution. Single-crystal X-ray diffraction reveals that the crystallography belongs to a triclinic PI space group with cell parameters: a = 0. 663 07(13) nm, b = 0. 898 39(18) nm, c = 1. 569 0(3) nm, α = 75.12(3)°,β = 87.92(3)°, γ = 77.57 (3)°. Antofloxacin shows no fluorescence in solution, but the crystalline state emits strong green light at 510 nm under the excitation of 360 nm, indicating a fluorescence enhancement induced by aggregation. It demonstrates that intermolecular packing and interaction in the crystal lead to the improved fluorescence quantum yield. These results provide important intbrmation for the further exploration of the structure-activity relationship of antofloxacin and the development of new drugs.
基金supported by the National Science Fund for Distinguished Young Scientists (No. 30925008)the Major International (Regional) Joint Research Project (No. 31010103901)+1 种基金the CAS-IOZ Innovation Program (KSCX2-EW-J-2) by a grant (No. O529YX5105) from the Key Laboratory of the Zoological Systematics and Evolution of the Chinese Academy of Sciences to F.M. Lei
文摘Although a growing number of both sequence-based and microsatellite nuclear loci have been used to infer genetic structures, their relative efficiencies remain poorly understood. In our study, we used the Green-backed Tit (Parus monticolus) to explore the resolving ability of these two types of markers. The south-western and central mitochondrial DNA (mtDNA) phylogroups were divergent to some extent in sequence-based nuclear data, while mixed together in microsatellites data. The F ST values among clades were about four times lower in microsatellite loci than those in sequence-based nuclear loci. We are of the opinion that size homoplasy may have contributed to the inability of microsatellites to uncover differentiation. Our results suggest that sequence-based nuclear loci outperformed microsatellite loci in detecting population structures, especially those focused on populations with large effective population sizes. There was no significant correlation between F ST values and allelic size variability, which suggested that the efficiency of microsatellite loci in detecting genetic structure may be independent of their polymorphism. F ST is better than R ST in detecting intraspecific divergence due to the high variance of R ST . In agreement with sequence-based nuclear loci, microsatellite loci did resolve the genetic distinctness of the Taiwan Residents phylogroup. The genetic differentiation between the Taiwan Residents and continental clades may involve allopatric divergence without gene flow.
基金National Science and Technology Action Program for Road Traffic Safety (No. 2009BAG13A05)the National Natural Science Foundation of China (No. 51078086)
文摘In order to evaluate the practicality and effectiveness of the turn-based algorithm for logit loading (TALL), the TALL is implemented using C++, and it is compared with a combination of the network-expanding method and the Dial algorithm based on the analysis of algorithm procedures. The TALL uses the arc-labeling shortest path searching, bidirectional star and the deque structure to directly assign the traffic flow, while the Dial algorithm should be used in an expanded network. The test results over realistic networks of eight cities show the superior performance of the TALL algorithm over the combination of the network-expanding method and the Dial algorithm, and the average processing time is reduced by 55. 4%. Furthermore, it is found that the operational efficiency of the TALL relates to the original densities of the cities. The average processing time is reduced by 65. 1% when the original density is about 14%, but the advantage of the TALL is not obvious with the increase in the original density.
文摘Monodisperse nanoparticle assembly with tunable structure, composition and properties can be taken as a superstructured building block for the construction of hierarchical nanostruc tures from the bottom up, which also represents a great challenge in nanotechnology. Here we report on a facile and controllable method that enables a high yield fabricatioa of uniform gold nanoparticle (AuNP) coresatellites with definable number (in average) of the satellite particles and tunable coretosatellite distance. The formation of the coresatellite nanostruc tures is driven by programmable DNAbasepairing, with the resulting nanocomplexes being isolatable via gel electrophoresis. By rationally controlling the DNA coverages on the core and shell particles, high production yields are achieved for the assembly/isolation process. As well, benefiting from a minimum DNA coverage on the satellite AuNPs, a strong affinity is observed for the asprepared coresatellites to get adsorbed on proteincoated graphene ox ide, which allows for a twodimensional hierarchical assembly of the coresatellite structures. The resulting hierarchical nanoassemblies are expected to find applications in various areas, including plasmonics, biosensing, and nanocatalysis. The method should be generalizable to make even more complicated and higherorder structures by making use of the structural programmability of DNA molecules.
基金supported by the National Natural Science Foundation of China(30870308 and 31071944)the Shanghai Rising-Star Program(10QA1402200)
文摘Cervus sichuanicus is a species of sika deer (Cervus nippon Group). To date, research has mainly focused on quantity surveying and behavior studies, with genetic information on this species currently deficient. To provide scientific evidence to assist in the protection of this species, we collected Sichuan sika deer fecal samples from the Sichuan Tiebu Nature Reserve (TNR) and extracted DNA from those samples. Microsatellite loci of bovine were used for PCR amplification. After GeneScan, the genotype data were used to analyze the genetic diversity and population structure of the Sichuan sika deer in TNR. Results showed that the average expected heterozygosity of the Sichuan sika deer population in TNR was 0.562, equivalent to the average expected heterozygosity of endangered animals, such as Procapra przewalsldi. Furthermore, 8 of 9 microsatellite loci significantly deviated from the Hardy-Weinberg equilibrium and two groups existed within the Sichuan sika deer TNR population. This genetic structure may be caused by a group of Manchurian sika deer (Cervus hortulorum) released in TNR.
基金Supported by the Key Project of Ministry of Education of China
文摘This paper is aimed to propose an approach to predict the dynamic characteristics of ring-plate planetary indexing cam mechanism, which is a novel type of indexing mechanism that employs internal planetary transmission structure. Firstly, the geometry and structure of the mechanism are discussed and the kinematic practicability is simulated with virtual prototype design. Then a 3D finite element model of the ring-plate planetary indexing cam mechanism is developed with the commercial software of MATLAB and ANSYS. Through the finite element analysis, the natural frequencies and the corresponding mode shapes are predicted in one motion cycle. On the basis of the virtual prototype design and finite element analysis, an experimental prototype is made and tested to validate the prediction of the dynamic characteristics. The agreement between experimental results and the finite element analysis testifies that the finite element model developed is applicable to the prediction of the dynamic characteristics of this type of mechanism.
基金supported by the National Natural Science Foundation of China (Grant No. 30530130)
文摘The Daurian Partridge(Perdix dauuricae) is a kind of hunting bird with high economic value.Genetic diversity and structure in the Daurian Partridge were studied by analyzing eight microsatellite loci in 23 populations found throughout the range of the species in China.The objectives were to evaluate the consequences on genetic diversity and differentiation of Daurian Partridge populations and to obtain a profound genetic insight for future management decisions and for effective measures to protect and exploit Daurian Partridges.The results showed that microsatellites were polymorphic in all Daurian Partridge populations,with a high level of genetic diversity over all the loci,especially in the Qaidam Basin populations which have the highest level of diversity.Significant genetic divergence was observed among different groups as well as between populations within the same group;most pairwise FST values were highly significant.Both phylogenetic trees and Bayesian clustering analyses revealed clear differentiation among the 23 populations of the Daurian Partridge,which were classified into two genetically differentiated groups.A bottleneck analysis indicated that Daurian Partridge populations have experienced a recent bottleneck.Our study argues that the Qaidam populations,North China populations,JN population,ZJC population,and Liupan Mountain populations should be paid special attention in order to retain adequate population sizes for maintaining genetic diversity.
基金supported by the National High Technology Research and Development Program of P.R.China under Grant No.2012 AA121604 the National Natural Science Foundation of China under Grants No.60902042,No.61170014,No.61202079+1 种基金 the National Research Foundation for the Doctoral Program of Higher Education of China under Grant No.20090006110014 the Foundation for Key Program of Ministry of Education of China under Grant No.311007
文摘The architecture of cislunar multi-hop communication networks, which focuses on the requirements of lunar full-coverage and continuous cislunar communications, is presented on the basis of Geosynchronous Orbit (GEO) satellite network relays. According to the geographical distribution of the forthcoming Chinese Deep Space Measuring and Controlling Network (DSMCN), two networking schemes are proposed and two elevation angle optimization models are established for locating GEO relay satellites. To analyze the dynamic connectivity, a dynamic network model is constructed with respect to the time-varying characteristics of cislunar trunk links. The advantages of the two proposed schemes, in terms of the Connectivity Rate (CR), Interruption Frequency (IF), and Average Length of Connecting Duration (ALCD), are corroborated by several simulations. In the case of the lunar polar orbit constellation case, the gains in the performance of scheme I are observed to be 134.55%, 117.03%, and 217.47% compared with DSMCN for three evaluation indicators, and the gains in the performance of scheme II are observed to be 238. 22%, 240.40%, and 572.71%. The results validate that the connectivity of GEO satellites outperforms that of earth facilities significantly and schemes based on GEO satellite relays are promising options for cislunar multi-hop communication networking.