临床检验过程中质量环节重要性的分析

质量控制措施在临床检验过程中具有重要的作用,它有利于提高检验结果的准确性、有利于提高医疗服务质量。当前在临床检验过程中,存在的主要问题是非病因素和科研人员会影响到检验结果的准确性。为了应对这些问题,加强质量控制,需要从临床检验前、检验过程、检验后采取相应质量控制措施。

Citrate-stabilized CdSe/CdS quantum dots as fluorescence probe for protein determination

A rapid, ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction of functionalized CdSe/CdS quantum dots （QDs） with bov/ne serum albumin （BSA） was proposed. The citrate-stabilized CdSe/CdS （QDs） were synthesized by using Se powder and Na2S as precursors instead of any pyrophoric organometallic precursors. The modified CdSe/CdS QDs are brighter and more stable against photobleaching in comparison with organic fluorophores. At pH 7.0, the fluorescence signal of CdSe/CdS is enhanced by increasing the concentration of BSA in the range of 0.1-10 μg/mL, and the low detection limit is 0.06 μg/mL. A linear relationship between the enhanced fluorescence peak intensity （△F） and BSA concentration （c） is established using equation △F=50.7c＋16.4 （R=0.996 36）. Results of determination for BSA in three synthetic samples are identical with the true values, and the recovery （98.9%-102.4%） and relative standard deviation （RSD, 1.8%-2.5%） are satisfactory.

Features and correct application of coal vitrinite reflectance index

The law of mathematical statistics, which the coal vitrinite reflectance index obeys, the existing vitrinite representing deviations, and the advantages of judging coalification and the shortage of judging coal type were analyzed. The advantages and disadvantages of various determination methods and the expression index and different methods of identifying vitrinite on the determination results were compared. The vitality of coal vitrinite reflectance depends on its full play the unique function of reflectance histogram and the systematic errors between different determination methods do not affect the application based on reflectance histogram are considered.

Determination of Hesperidin and Limonin Levels as Part of the Defense Mechanism of Some Lemon Varieties [Citrus limon （L,） Burm f.] against Mal Secco Disease [Phoma tracheiphila （Petri） Kanc,et Ghik,]

In this paper, it was aimed to identified and quantified hesperidin and limonin compounds using HPLC （High Performance Liquid Chromatography） techniques against to developing of mal secco disease caused by Phoma tracheiphila. Six citrus lemon varieties （Meyer, Kiitdiken, Enterdonato, Yediveren, Sweet lemon and Euroka） were infected by P. tracheiphila and artificial inoculation were applied in vivo conditions. Before and after inoculation, leaf, branch and stem samples were taken from each lemon varieties. The results show that the amount of hesperidin and limonin concentration was increased after the inoculations at various levels based on the lemon cultivars. Various concentrations （1, 5, 10, 25, 50, 75, 100 ppm） of hesperidin and limonin compounds were also tested under in vitro conditions to compare response of P. tracheiphila development. According to the results, hesperidin and limonin compounds play an important role against to P. tracheiphila development and Sweet Lemon variety was found to be the most resistance both observation and HPLC results.

Evaluation of mouse bioassay results in an inter-laboratory comparison for paralytic shellfish poisoning toxins

An inter-laboratory comparison of the AOAC mouse bioassay for paralytic shellfish poisoning (PSP) toxicity in shellfish was carried out among 25 Chinese laboratories to examine the overall performance for PSP testing in China, and to analyze the main factors affecting the performance of this method. The toxic scallop Patinopecten yessoensis collected from coast of Bohai Sea, China, was used as a test sample in the comparison study. The results were reported and evaluated using robust statistical methods. The z scores showed that 80%, 8%, and 12% of laboratories reported satisfactory results, unsatisfactory results, and questionable results, respectively. This evaluation demonstrates that the PSP mouse bioassay is an appropriate method for screening and testing PSP toxicity in shellfish. However, it was found that the experience and skill of technicians, as well as the body weight and health status of mice being used significantly affected the accuracy of the method.

New Silver Nanosensor for Nickel Traces. Part II： Urinary Nickel Determination Associated to Smoking Addiction

A new fluorescence silver nanosensor assisted by surfactant has been recently synthesized and applied to ultra trace nickel determination. The methodology was validated by the standard addition method and satisfactorily applied to nickel determination in urine without previous treatment, coming from subjects with different smoking addiction levels and second hand smokers. Within-day precision was better than 0.011 CV. The reproducibility （between-days precision） was also evaluated over 3 days by performing six determinations each day with a CV of 0.025. The proposed methodology represents a promising approach in the area of biological monitoring due to its low operation cost, simplicity of instrumentation, high sampling speed and non-polluting solvents. Obtained results of urinary nickel concentration were successfully correlated with the tobacco addiction.

Homemade versus Commercial Jarred Baby Foods with Regard to Nitrites and Nitrates Content

This paper presents results of nitrites and nitrates determination in two types of baby foods： commercial products in jars and their homemade conventional counterparts. Nitrites levels in all analyzed samples were below of the detection limit （〈 0.9 mg/kg） of applied spectrophotometric method with Griess reagent. Nitrates contents in commercial products ranged： 9.1-38.1 mg/kg while in homemade baby foods levels between 26.6 mg/kg and 118.8 mg/kg were obtained. All the contents of nitrates were lower than the EU legislation maximum limit （200 mg/kg）. Comparison of each type of commercial product with its homemade counterpart baby food evidenced significant differences （p 〈 0.05） in average nitrates levels in favor of the first type. Apart from determining and comparing the levels of nitrates in the baby food samples also risk assessment for an average 6-months old infant to nitrates exposure was conducted. The estimated nitrates intake with a typical portion of 200g of baby food ranged between 6% and 25.7% of acceptable daily intake for commercial and from 18.0% to 80.3% for homemade ones.

Investigation of Cutting Force by End Milling Operation

In this work, the cutting forces by end milling operation are analyzed. Therefore, the main parameters of cutting force as cutting speed, feed rate and depth of cut also are investigated in our case. The cutting force is modelled and analyzed into mathematical Wolfram simulations in order to compare the results and in the same time achieve the best solutions. Theoretical results are carried out by using the regression method that required fulfilling the critter by Fisher. The number of experiment, measurements and results of cutting force are presented in 2D as well as 3D. In order to verify the accuracy of the 2D diagram, the results for our case is used both two way such as experimental and theoretical method as well as results are compared. In other hands, these results indicate directly that the optimized parameters are capable of machining the workpiece. The obtained measurement results are compared with theoretical methods in Wolfram software.

Stabilization of Alliinase from Garlic by Osmolytes and the Mannose-Specific Lectin ASAI

Objectives： Alliinase is a pyridoxal-5＇-phosphate （PLP）-dependent enzyme responsible for the production of diallyl thiosulfinate （allicin）, the biologically active component of garlic, from alliin. The use of allicin for treatment of various diseases has been proposed but it is very unstable in the blood stream. This difficulty can be overcome by administration of alliin, together with a conjugate of alliinase directed towards the target cells. This, in turn requires a stable and active form of the enzyme. In this study we evaluate the stability of alliinase itself, in the presence mad absence of osmolytes, as well as that of its catalytically active complex with a mannose-specific lectin, ASAI （Allium sativum agglutinin I）, also presents in garlic. Methods： Alliinase, mad ASAI were both purified from garlic cloves. Thermal stability of alliinase itself, mad of its complexes with PLP and ASAI, in the presence mad absence of osmolytes, was analyzed by monitoring enzymic activity, and using DSC （differential scanning calorimetry）. Key findings： PLP exerts only a minor influence on alliinase structure and stablity. But both osmolytes and ASAI stabilize the enzyme considerably. Conclusions： The principle finding is that ASAI greatly stabilizes alliinase. Thus, the lectin-enzyme complex, which can be lyophilized and stored until used, provides an effective formulation of alliinase for generation of allicin from alliin in vivo.

Energy and Autohydrolysis By-Products from Industrial Crops

The world is experiencing a growing shortage of raw materials which is especially severe in the energy sector and being worsened by the unfavorable environmental impact of a consumerist cultttre revolving around the exploitation of non-renewable resources. In this work, the calorific value and chemical composition of liquors resulting from the autohydrolysis of six different lignocellulosic materials was determined （Eucalyptus globulus, Arundo donax, Leucaena diversifolia, Paulownia fortunei, Sunflower stalks and Chamaecytisus proliferus） and was assessed for the obtainment of energy, sugars and other chemical products by using of integral fractionation based on autohydrolysis. Autohydrolysis processes have been considered interesting in the case of timber species studied compared with herbaceous species with variations in the extraction of the xylan fraction at 180℃ between 19.68% for Eucalyptus globulus and 36.79% for Leucaena diversifolia and 200℃ between 57.86% for Paulawniafortunei and 79.13 % for Chamaecytisusproliferus. In general, all materials show a solid fraction ＂more energy＂ from the hydrolysis to 200℃ than 180℃ and raw materials. It is interesting as the potential economy valuation of the liquid fractions of the hydrolysis, the solid waste recovery present more energetic profitability.

Continuous on-line adaptation of the rotor time constant in FOC induction machine system

Rotor time constant is an important parameter for the indirect lleld oraentateO control of mauc- tion motor. Incorrect rotor tittle constant value will cause the flux observer generating a wrong angu- lar orientation of the rotor field. A new approach serves for rotor time constant on-line adaptation by setting the stator current to be zero for a short period. A smooth eorrector is designed to prevent ab- normal detection result from making adaptation. Impact of zero current duration on detection error and rotor speed is analyzed by experiments.

A new damping ratio identification method based on pattern search

In order to improve the effectiveness of traditional time domain identification methods in identifying damping ratios, a new damping ratio identification method based on pattern search is proposed by fluctuating the reliable natural frequency obtained through traditional time domain identification methods by about 10% to build the boundary conditions, using all the initial identification results to establish the free decay response of the system, and using the pattern search method to correct the initial identification results with the residual sum of squares between the free decay response and the actually measured free-decay signal as the objective function. The proposed method deals with the actually measured free-decay signal with curve fitting and avoids enlarging the identified error caused by intermediate conversion, so it can effectively improve the identified accuracy of damping ratios. Simulations for a room-sized vibration isolation foundation show that the relative errors of analyzed three damping ratios are down to 1.05%, 1.51% and 3.7% by the proposed method from 8.42%, 5.85% and 8.5% by STD method when the noise level is 10%.

Detecting Factor Ⅺ Deficiency in Holstein Cattle Using PCR Analysis

[Objective] This study established a method to detect Factor Ⅺ by polymerase chain reaction analysis.[Method]A pair of primers was designed and synthesized according to sequences of FⅪ gene in Holstein calves,published in Genbank. Polymerase chain reaction was used to analyze FⅪ deficiency of 576 Holstein calves in Henan,and the result was verified by DNA sequencing. [Result] We detect 576 cows,which include two carriers and one F Ⅺ deficiency,and the result was consistent with the DNA sequencing. The frequency of the FⅪ mutant allele was 0.3%,the carrier was 0.3%,the prevalence was 0.2%.[Conclusion]A method detecting FⅪ by polymerase chain reaction analysis was established. This method is not only simple and convenient,but also has a high accuracy and low cost,which is more suitable for large-scale FⅪ investigation.

Image J在两种痔疮动物模型量化分析中的应用

目的:评价Image J应用于两种痔疮动物模型图像量化分析的适用性。方法:基于经典的大鼠巴豆油肛门肿胀模型和醋酸致肛门溃疡模型,分别以临床阳性药物痔疮胶囊(口服)和马应龙痔疮膏(外用)两种治疗方法,比较Image J图像分析法用于实验性痔疮研究的适用性和科学性。结果:相较传统方法,Image J方法处理过程可控,具有较好的可重复性和可追溯性,能客观、准确地反映病变程度。结论:Image J程序平台适用于痔疮动物模型图像数据的量化分析,该数据处理方法更为灵活简便,节省动物模型,结果科学可信。

Friction anisotropy dependence on lattice orientation of graphene

The observation of friction anisotropy on graphene by friction measurement at atomic scale has been reported in this paper.Atomic-scale friction measurement revealed friction anisotropy with a periodicity of 60°,which is consistent with the hexagonal periodicity of the graphene.Both experiments and theory show that the value of the friction force is related to the graphene lattice orientation,and the friction force along armchair orientation is also larger than the one along zigzag orientation.These results will play a critical role in the use of graphene to manufacture nanoscale devices.

ON ASYMPTOTIC NORMALITY OF PARAMETERS IN MULTIPLE LINEAR ERRORS-IN-VARIABLES MODEL

This paper studies the parameter estimation of multiple dimensional linear errors-in-variables (EV) models in the case where replicated observations are available in some experimental points. Asymptotic normality is established under mild conditions, and the parameters entering the asymptotic variance are consistently estimated to render the result useable in the construction of large-sample confidence regions.

Entangler and analyzer for multiphoton Greenberger-Horne-Zeilinger states using weak nonlinearities

In the regime of weak nonlinearity we present two general,feasible schemes for manipulating photon states.One is an entangler for generating any one of the n-photon Greenberger-Horne-Zeilinger(GHZ)states.Interactions of the incoming photons with crossKerr media followed by a phase shift gate and a measurement on a probe beam plus appropriate local operations using classical feed-forward of the measurement results allow one to obtain the desired states in a nearly deterministic manner.The second scheme discussed is an analyzer for multiphoton maximally entangled states,which is derived from the above entangler.In this scheme,all of the 2nn-photon GHZ states can,nearly deterministically,be discriminated.